Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ADP-ribosylation factor GTPase-activating protein AGD12

Gene

AGD12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTPase-activating protein (GAP) for ADP ribosylation factor (ARF). Binds phosphatidylinositol 3-monophosohate (PI-3-P) and anionic phospholipids.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri30 – 53C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • GTPase activator activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • phospholipid binding Source: TAIR

GO - Biological processi

  • intracellular protein transport Source: TAIR

Keywordsi

Molecular functionGTPase activation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor GTPase-activating protein AGD12
Short name:
ARF GAP AGD12
Alternative name(s):
Protein ARF-GAP DOMAIN 12
Short name:
AtAGD12
Zinc- and calcium-binding protein
Short name:
AtZAC
Gene namesi
Name:AGD12
Synonyms:ZAC
Ordered Locus Names:At4g21160
ORF Names:F7J7.100
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G21160
TAIRilocus:2127338 AT4G21160

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi33C → S: Loss of zinc and PI-3-P binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003525031 – 337ADP-ribosylation factor GTPase-activating protein AGD12Add BLAST337

Proteomic databases

PaxDbiQ9FVJ3

PTM databases

iPTMnetiQ9FVJ3

Expressioni

Tissue specificityi

Expressed in roots, leaves, flowers and siliques. Low levels of expression in seeds and stems.1 Publication

Gene expression databases

ExpressionAtlasiQ9FVJ3 baseline and differential
GenevisibleiQ9FVJ3 AT

Interactioni

Protein-protein interaction databases

BioGridi13155, 1 interactor
IntActiQ9FVJ3, 1 interactor
STRINGi3702.AT4G21160.1

Structurei

3D structure databases

ProteinModelPortaliQ9FVJ3
SMRiQ9FVJ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 137Arf-GAPPROSITE-ProRule annotationAdd BLAST123
Domaini168 – 262C2PROSITE-ProRule annotationAdd BLAST95

Domaini

The C2 domain binds anionic phospholipids promiscuously in the presence of calcium while the N-terminal region (1-174) show a specific affinity for phosphatidylinositol 3-monophosohate.

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri30 – 53C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0703 Eukaryota
KOG1030 Eukaryota
COG5347 LUCA
HOGENOMiHOG000238659
InParanoidiQ9FVJ3
KOiK12486
OMAiHNYGSVK
OrthoDBiEOG09360GE9
PhylomeDBiQ9FVJ3

Family and domain databases

Gene3Di2.60.40.150, 1 hit
3.30.40.160, 1 hit
InterProiView protein in InterPro
IPR037278 ARFGAP/RecO
IPR001164 ArfGAP_dom
IPR038508 ArfGAP_dom_sf
IPR000008 C2_dom
IPR035892 C2_domain_sf
PfamiView protein in Pfam
PF01412 ArfGap, 1 hit
PF00168 C2, 1 hit
PRINTSiPR00405 REVINTRACTNG
SMARTiView protein in SMART
SM00105 ArfGap, 1 hit
SM00239 C2, 1 hit
SUPFAMiSSF57863 SSF57863, 1 hit
PROSITEiView protein in PROSITE
PS50115 ARFGAP, 1 hit
PS50004 C2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9FVJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYSGAGLGK PGSGKRRIRD LLTQSDNRVC ADCGAPDPKW ASANIGVFIC
60 70 80 90 100
LKCCGVHRSL GSHISKVLSV TLDEWSDEEV DSMIEIGGNA SANSIYEAFI
110 120 130 140 150
PEGSSKPGPD ASHDQRMRFI RSKYEHQEFL KPSLRITSVR GSSTKTPAFL
160 170 180 190 200
SSSLSKKIVD SFRTNSSSQQ PQLEGMVEFI GLLKVTIKKG TNMAIRDMMS
210 220 230 240 250
SDPYVVLTLG QQKAQSTVVK SNLNPVWNEE LMLSVPHNYG SVKLQVFDYD
260 270 280 290 300
TFSADDIMGE AEIDIQPLIT SAMAFGDPEM FGDMQIGKWL KSHDNALIED
310 320 330
SIINIADGKV KQEVQIKLQN VESGELELEM EWLPLEQ
Length:337
Mass (Da):37,145
Last modified:March 1, 2001 - v1
Checksum:iA2E56C4F66FE9FE1
GO

Sequence cautioni

The sequence CAA17535 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB79116 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti141G → V in AAM65970 (Ref. 5) Curated1
Sequence conflicti214A → V in BAF01268 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177381 mRNA Translation: AAG09280.1
AL021960 Genomic DNA Translation: CAA17535.1 Sequence problems.
AL161554 Genomic DNA Translation: CAB79116.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE84411.1
CP002687 Genomic DNA Translation: AEE84412.1
CP002687 Genomic DNA Translation: AEE84413.1
CP002687 Genomic DNA Translation: AEE84414.1
AY062549 mRNA Translation: AAL32627.1
BT008822 mRNA Translation: AAP68261.1
AY088434 mRNA Translation: AAM65970.1
AK229406 mRNA Translation: BAF01268.1
PIRiT04947
RefSeqiNP_567620.1, NM_118235.4
NP_849416.1, NM_179085.3
NP_974581.1, NM_202852.2
NP_974582.1, NM_202853.3
UniGeneiAt.75195

Genome annotation databases

EnsemblPlantsiAT4G21160.1; AT4G21160.1; AT4G21160
AT4G21160.2; AT4G21160.2; AT4G21160
AT4G21160.3; AT4G21160.3; AT4G21160
AT4G21160.4; AT4G21160.4; AT4G21160
GeneIDi827864
GrameneiAT4G21160.1; AT4G21160.1; AT4G21160
AT4G21160.2; AT4G21160.2; AT4G21160
AT4G21160.3; AT4G21160.3; AT4G21160
AT4G21160.4; AT4G21160.4; AT4G21160
KEGGiath:AT4G21160

Similar proteinsi

Entry informationi

Entry nameiAGD12_ARATH
AccessioniPrimary (citable) accession number: Q9FVJ3
Secondary accession number(s): O49557, Q0WNN1, Q8L9H2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health