Q9FVG8 (METK1_PINCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-adenosylmethionine synthase 1
Short name=AdoMet synthase 1
EC=2.5.1.6

Alternative name(s):
Methionine adenosyltransferase 1
Short name=MAT 1

Gene names

Name:

SAMS1

Organism

Pinus contorta (Shore pine) (Lodgepole pine)

Taxonomic identifier

3339 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Coniferopsida › Coniferales › Pinaceae › Pinus › Pinus

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity.
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Mostly in Roots. Ref.1
Developmental stage	Induced in the cells forming the adventitious root primordium in connection to the primary xylem. Accumulates in the protruding adventitious roots. Ref.1
Sequence similarities	Belongs to the AdoMet synthase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Gene Ontology (GO)
Biological process	S-adenosylmethionine biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine adenosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	S-adenosylmethionine synthase 1		PRO_0000363040
Regions
	Nucleotide binding	120 – 125	6	ATP Potential
	Nucleotide binding	265 – 272	8	ATP Potential
Sites
	Metal binding	19	1	Magnesium By similarity
	Metal binding	45	1	Potassium By similarity
	Metal binding	269	1	Potassium By similarity
	Metal binding	277	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FVG8 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 43645A8BF4FF9816	FASTA	388	42,315
	10 20 30 40 50 60 MFIMTKYFTS ESVSAGHPDK IADQIADAIL DAVLAQDPYA RSAVEVTTST GDVSIFGELS 70 80 90 100 110 120 TSAYVNVRQI AMDTIREIGY NQAELGFTAD SVNVSNRIVE QSGDIAQAVD SADDDPEQLG 130 140 150 160 170 180 AGDQGMVFGY ATNETDNYLP LTLALSHRLM RQIRDVREAG TLNYLRPDAK AEVSVELDDN 190 200 210 220 230 240 NQVSRIAAVV LSTQHDETVT LDQLREDVRR LVIDPVLPQE LVDDETIYYI NPSGRFVLGG 250 260 270 280 290 300 PQADSGLTGR KIIVDTYGGA AHHGGGAFSG KDATKVDRSA AYFARYVAKN LVAAGVADKL 310 320 330 340 350 360 ELQVAYAIGV AQPVSLNIET FGTAKIDEDK IRDITAQLFD FRPLAIINHL DLRRPIYKQT 370 380 AAFGHFGRTD IDLPWEALDQ VDKIKALM

References

[1]	"Two S-adenosylmethionine synthetase-encoding genes differentially expressed during adventitious root development in Pinus contorta." Lindroth A.M., Saarikoski P., Flygh G., Clapham D., Groenroos R., Thelander M., Ronne H., von Arnold S. Plant Mol. Biol. 46:335-346(2001) [PubMed: 11488480] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Root.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF187820 mRNA. Translation: AAG17035.1.
3D structure databases
HSSP	HSSP built from PDB template 1RG9 based on UniProtKB P0A817.
ProteinModelPortal	Q9FVG8.
SMR	Q9FVG8. Positions 6-387.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR022631. ADOMET_SYNTHASE_CS. IPR022630. S-AdoMet_synt_C. IPR022629. S-AdoMet_synt_central. IPR022628. S-AdoMet_synt_N. IPR002133. S-AdoMet_synthetase. IPR022636. S-AdoMet_synthetase_sfam. [Graphical view]
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
SUPFAM	SSF55973. S-AdoMet_synt. 3 hits.
TIGRFAMs	TIGR01034. MetK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHASE_1. 1 hit. PS00377. ADOMET_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METK1_PINCO

Accession

Primary (citable) accession number: Q9FVG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	March 1, 2001
Last modified:	June 28, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program