Reviewed, UniProtKB/Swiss-Prot Q9FVC8 (DAPA2_ARATH)

Names and origin

Protein names

Recommended name:
    Dihydrodipicolinate synthase 2, chloroplastic
      Short name=DHDPS 2
    EC=4.2.1.52

Gene names

Name:	DHDPS2
Ordered Locus Names:	At2g45440
ORF Names:	F4L23.5

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Schiff base
Molecular function	Lyase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from direct assay. Source: TAIR
Molecular function	dihydrodipicolinate synthase activity Ref.1 Inferred from mutant phenotype. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 39	39	Chloroplast Potential
	Chain	40 – 365	326	Dihydrodipicolinate synthase 2, chloroplastic		PRO_0000007198
Sites
	Active site	222	1	Schiff-base intermediate with substrate By similarity
	Binding site	169	1	Pyruvate By similarity
	Site	194	1	Involved in proton transfer during cleavage By similarity
Experimental info
	Sequence conflict	26	1	S → G in AAG28565. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9FVC8-1 [UniParc]. Last modified June 20, 2001. Version 2. Checksum: 43BECEF1FC3E557A	FASTA	365	40,292
	10 20 30 40 50 60 MAALKGYGLC SMDSALQFPC PKLFNSYKRR SSKWVSPKAA VVPNFHLPMR SLEVKNRTNT 70 80 90 100 110 120 DDIKALRVIT AIKTPYLPDG RFDLEAYDDL VNIQIQNGAE GVIVGGTTGE GQLMSWDEHI 130 140 150 160 170 180 MLIGHTVNCF GGSIKVIGNT GSNSTREAIH ATEQGFAVGM HAALHINPYY GKTSIEGLIA 190 200 210 220 230 240 HFQSVLHMGP TIIYNVPGRT GQDIPPRAIF KLSQNPNLAG VKECVGNKRV EEYTENGVVV 250 260 270 280 290 300 WSGNDDECHD SRWDYGATGV ISVTSNLVPG LMRKLMFEGR NSSLNSKLLP LMAWLFHEPN 310 320 330 340 350 360 PIGINTALAQ LGVSRPVFRL PYVPLPLSKR LEFVKLVKEI GREHFVGEKD VQALDDDDFI LIGRY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of an Arabidopsis thaliana mutant accumulating threonine resulting from mutation in a new dihydrodipicolinate synthase gene." Sarrobert C., Thibaud M.-C., Contard-David P., Gineste S., Bechtold N., Robaglia C., Nussaume L. Plant J. 24:357-367(2000) [PubMed: 11069709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Wassilewskija.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF200325 Genomic DNA. Translation: AAG28565.1. AC002387 Genomic DNA. Translation: AAB82620.1. BT004823 mRNA. Translation: AAO44089.1.
IPI	IPI00541031.
PIR	E84890.
RefSeq	NP_182068.1.
UniGene	At.12311
3D structure databases
HSSP	HSSP built from PDB template 1DHP based on UniProtKB P05640.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9FVC8.
Proteomic databases
PRIDE	Q9FVC8.
Genome annotation databases
GeneID	819152.
GenomeReviews	Gene locus AT2G45440 in contig CT485783_GR.
KEGG	ath:AT2G45440.
NMPDR	fig|3702.1.peg.11720.
Organism-specific databases
TAIR	At2g45440.
Phylogenomic databases
OMA	QGWQVGS.
Enzyme and pathway databases
BRENDA	4.2.1.52. 302.
Gene expression databases
ArrayExpress	Q9FVC8.
Genevestigator	Q9FVC8.
GermOnline	AT2G45440. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR005263. DapA_synth. IPR002220. DHDPS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PRINTS	PR00146. DHPICSNTHASE.
ProDom	PD001859. DHDPS. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00674. dapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA2_ARATH

Accession

Primary (citable) accession number: Q9FVC8
Secondary accession number(s): O22129

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	June 20, 2001
Last modified:	November 3, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)