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Protein

Transcription factor E2FB

Gene

E2FB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The binding of retinoblastoma-related proteins represses transactivation. Involved in the control of cell-cycle progression from G1 to S phase and from G2 to M phase. Stimulates cell proliferation and delays differentiation. Represses cell enlargement and endoreduplication in auxin-free conditions.7 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi129 – 19466Sequence analysisAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • positive regulation of cell proliferation Source: UniProtKB
  • positive regulation of meiotic cell cycle Source: TAIR
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of cell division Source: TAIR
  • response to auxin Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
  • trichome morphogenesis Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-ATH-113510. E2F mediated regulation of DNA replication.
R-ATH-1538133. G0 and Early G1.
R-ATH-68689. CDC6 association with the ORC:origin complex.
R-ATH-68911. G2 Phase.
R-ATH-69205. G1/S-Specific Transcription.
R-ATH-69298. Association of licensing factors with the pre-replicative complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2FB
Alternative name(s):
E2F transcription factor-1
Short name:
AtE2F1
Gene namesi
Name:E2FB
Synonyms:E2F1
Ordered Locus Names:At5g22220
ORF Names:T6G21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G22220.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Interaction with DPA induces an exclusive nuclear localization, but an interaction with DPB has no effect.

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • nucleus Source: TAIR
  • transcription factor complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Transcription factor E2FBPRO_0000406290Add
BLAST

Post-translational modificationi

Phosphorylated.1 Publication

Proteomic databases

PaxDbiQ9FV71.
PRIDEiQ9FV71.

PTM databases

iPTMnetiQ9FV71.

Expressioni

Tissue specificityi

Expressed in proliferating cells and several differentiated tissues. Detected in inflorescence and shoot apical meristems, cotyledonary vascular tissues, leaf primordia, young leaves, base of trichomes, central cylinder and elongation zone of roots, lateral root primordia, flowers, pistils of immature flowers and pollen grains.1 Publication

Developmental stagei

Expressed in a cell cycle-dependent manner. Most abundant at the G1/S transition. Lower but constant level in the following phases.3 Publications

Inductioni

Up-regulated by light and by auxin. May be up-regulated by E2FA.3 Publications

Gene expression databases

GenevisibleiQ9FV71. AT.

Interactioni

Subunit structurei

Heterodimer with DP proteins. Interacts (via dimerization domain) preferentially with DPA, but also with DPB. Interacts with PURA1 and retinoblastoma-related protein RBR1.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ATPK1P428183EBI-1774719,EBI-8107038
DPAQ9FNY310EBI-1774719,EBI-1774763
DPBQ9FNY26EBI-1774719,EBI-1774876
RBR1Q9LKZ34EBI-1774719,EBI-398590

Protein-protein interaction databases

BioGridi17558. 8 interactions.
IntActiQ9FV71. 11 interactions.
MINTiMINT-8050696.
STRINGi3702.AT5G22220.2.

Structurei

3D structure databases

ProteinModelPortaliQ9FV71.
SMRiQ9FV71. Positions 129-193, 207-310.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni210 – 23829Leucine-zipperSequence analysisAdd
BLAST
Regioni403 – 41917Retinoblastoma protein bindingSequence analysisAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili202 – 24645Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2577. Eukaryota.
ENOG410XNYI. LUCA.
HOGENOMiHOG000232044.
KOiK06620.
OMAiTERNMET.
OrthoDBiEOG09360AY6.
PhylomeDBiQ9FV71.

Family and domain databases

CDDicd14660. E2F_DD. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR015633. E2F.
IPR032198. E2F_CC-MB.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12081. PTHR12081. 1 hit.
PfamiPF16421. E2F_CC-MB. 1 hit.
PF02319. E2F_TDP. 1 hit.
[Graphical view]
SMARTiSM01372. E2F_TDP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9FV71-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEEVPQQFP SSKRQLHPSL SSMKPPLVAP GEYHRFDAAE TRGGGAVADQ
60 70 80 90 100
VVSDAIVIKS TLKRKTDLVN QIVEVNELNT GVLQTPVSGK GGKAKKTSRS
110 120 130 140 150
AKSNKSGTLA SGSNAGSPGN NFAQAGTCRY DSSLGLLTKK FINLIKQAED
160 170 180 190 200
GILDLNKAAD TLEVQKRRIY DITNVLEGIG LIEKTLKNRI QWKGLDVSKP
210 220 230 240 250
GETIESIANL QDEVQNLAAE EARLDDQIRE SQERLTSLSE DENNKRLLFV
260 270 280 290 300
TENDIKNLPC FQNKTLIAVK APHGTTLEVP DPDEAGGYQR RYRIILRSTM
310 320 330 340 350
GPIDVYLVSQ FEESFEDIPQ ADEPSNVPDE PSNVPDVPSN LPSTSGLPEN
360 370 380 390 400
HDVSMPMKEE STERNMETQE VDDTQRVYSD IESHDFVDGI MKIVPPDLDM
410 420 430 440 450
GVDYWFRSEV GEVSITDMWP DESGPDWNQM ITFDQDHAGP SDNKILEQPQ
460
TPSSPTPEES TATRSPTGS
Length:469
Mass (Da):51,653
Last modified:March 1, 2001 - v1
Checksum:iADE1104B34651B7C
GO
Isoform 2 (identifier: Q9FV71-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     423-426: SGPD → Y

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:466
Mass (Da):51,460
Checksum:i8AEE8FFCBA700052
GO

Sequence cautioni

The sequence BAB17029 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti337 – 3371V → E in CAC15485 (PubMed:11108847).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei423 – 4264SGPD → Y in isoform 2. CuratedVSP_040804

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ294533 mRNA. Translation: CAC15485.1.
AF242580 mRNA. Translation: AAG17608.1.
AL589883 Genomic DNA. Translation: CAC34515.1.
AB007651 Genomic DNA. Translation: BAB17029.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92998.1.
CP002688 Genomic DNA. Translation: AED92999.1.
AY136479 mRNA. Translation: AAM97144.1.
BT006610 mRNA. Translation: AAP31954.1.
RefSeqiNP_001031921.3. NM_001036844.3. [Q9FV71-2]
NP_568413.1. NM_122128.2. [Q9FV71-1]
UniGeneiAt.10189.

Genome annotation databases

EnsemblPlantsiAT5G22220.2; AT5G22220.2; AT5G22220. [Q9FV71-1]
GeneIDi832283.
KEGGiath:AT5G22220.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ294533 mRNA. Translation: CAC15485.1.
AF242580 mRNA. Translation: AAG17608.1.
AL589883 Genomic DNA. Translation: CAC34515.1.
AB007651 Genomic DNA. Translation: BAB17029.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92998.1.
CP002688 Genomic DNA. Translation: AED92999.1.
AY136479 mRNA. Translation: AAM97144.1.
BT006610 mRNA. Translation: AAP31954.1.
RefSeqiNP_001031921.3. NM_001036844.3. [Q9FV71-2]
NP_568413.1. NM_122128.2. [Q9FV71-1]
UniGeneiAt.10189.

3D structure databases

ProteinModelPortaliQ9FV71.
SMRiQ9FV71. Positions 129-193, 207-310.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17558. 8 interactions.
IntActiQ9FV71. 11 interactions.
MINTiMINT-8050696.
STRINGi3702.AT5G22220.2.

PTM databases

iPTMnetiQ9FV71.

Proteomic databases

PaxDbiQ9FV71.
PRIDEiQ9FV71.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G22220.2; AT5G22220.2; AT5G22220. [Q9FV71-1]
GeneIDi832283.
KEGGiath:AT5G22220.

Organism-specific databases

TAIRiAT5G22220.

Phylogenomic databases

eggNOGiKOG2577. Eukaryota.
ENOG410XNYI. LUCA.
HOGENOMiHOG000232044.
KOiK06620.
OMAiTERNMET.
OrthoDBiEOG09360AY6.
PhylomeDBiQ9FV71.

Enzyme and pathway databases

ReactomeiR-ATH-113510. E2F mediated regulation of DNA replication.
R-ATH-1538133. G0 and Early G1.
R-ATH-68689. CDC6 association with the ORC:origin complex.
R-ATH-68911. G2 Phase.
R-ATH-69205. G1/S-Specific Transcription.
R-ATH-69298. Association of licensing factors with the pre-replicative complex.

Miscellaneous databases

PROiQ9FV71.

Gene expression databases

GenevisibleiQ9FV71. AT.

Family and domain databases

CDDicd14660. E2F_DD. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR015633. E2F.
IPR032198. E2F_CC-MB.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12081. PTHR12081. 1 hit.
PfamiPF16421. E2F_CC-MB. 1 hit.
PF02319. E2F_TDP. 1 hit.
[Graphical view]
SMARTiSM01372. E2F_TDP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiE2FB_ARATH
AccessioniPrimary (citable) accession number: Q9FV71
Secondary accession number(s): Q9FMS8, Q9FNY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.