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Protein

Transcription factor E2FB

Gene

E2FB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The binding of retinoblastoma-related proteins represses transactivation. Involved in the control of cell-cycle progression from G1 to S phase and from G2 to M phase. Stimulates cell proliferation and delays differentiation. Represses cell enlargement and endoreduplication in auxin-free conditions.7 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi129 – 194Sequence analysisAdd BLAST66

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • DNA binding transcription factor activity Source: TAIR
  • protein dimerization activity Source: InterPro

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • positive regulation of cell proliferation Source: UniProtKB
  • positive regulation of meiotic cell cycle Source: TAIR
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of cell division Source: TAIR
  • response to auxin Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
  • trichome morphogenesis Source: TAIR

Keywordsi

Molecular functionActivator, DNA-binding
Biological processCell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-ATH-1538133 G0 and Early G1
R-ATH-68689 CDC6 association with the ORC:origin complex
R-ATH-68911 G2 Phase
R-ATH-69231 Cyclin D associated events in G1
R-ATH-8953750 Transcriptional Regulation by E2F6

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2FB
Alternative name(s):
E2F transcription factor-1
Short name:
AtE2F1
Gene namesi
Name:E2FB
Synonyms:E2F1
Ordered Locus Names:At5g22220
ORF Names:T6G21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G22220
TAIRilocus:3685148 AT5G22220

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004062901 – 469Transcription factor E2FBAdd BLAST469

Post-translational modificationi

Phosphorylated.1 Publication

Proteomic databases

PaxDbiQ9FV71

PTM databases

iPTMnetiQ9FV71

Expressioni

Tissue specificityi

Expressed in proliferating cells and several differentiated tissues. Detected in inflorescence and shoot apical meristems, cotyledonary vascular tissues, leaf primordia, young leaves, base of trichomes, central cylinder and elongation zone of roots, lateral root primordia, flowers, pistils of immature flowers and pollen grains.1 Publication

Developmental stagei

Expressed in a cell cycle-dependent manner. Most abundant at the G1/S transition. Lower but constant level in the following phases.3 Publications

Inductioni

Up-regulated by light and by auxin. May be up-regulated by E2FA.3 Publications

Gene expression databases

ExpressionAtlasiQ9FV71 baseline and differential
GenevisibleiQ9FV71 AT

Interactioni

Subunit structurei

Heterodimer with DP proteins. Interacts (via dimerization domain) preferentially with DPA, but also with DPB. Interacts with PURA1 and retinoblastoma-related protein RBR1. Component of a DREAM-like complex which modulates a variety of developmentally regulated genes and of the mitotic genes in proliferating and differentiated cells. Interacts with MYB3R4 only at early stages of leaves development (PubMed:26069325).7 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi17558, 9 interactors
IntActiQ9FV71, 11 interactors
MINTiQ9FV71
STRINGi3702.AT5G22220.2

Structurei

3D structure databases

ProteinModelPortaliQ9FV71
SMRiQ9FV71
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni210 – 238Leucine-zipperSequence analysisAdd BLAST29
Regioni403 – 419Retinoblastoma protein bindingSequence analysisAdd BLAST17

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili202 – 246Sequence analysisAdd BLAST45

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2577 Eukaryota
ENOG410XNYI LUCA
HOGENOMiHOG000232044
KOiK06620
OMAiTRRISWK
OrthoDBiEOG09360AY6
PhylomeDBiQ9FV71

Family and domain databases

CDDicd14660 E2F_DD, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR015633 E2F
IPR037241 E2F-DP_heterodim
IPR032198 E2F_CC-MB
IPR003316 E2F_WHTH_DNA-bd_dom
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12081 PTHR12081, 1 hit
PfamiView protein in Pfam
PF16421 E2F_CC-MB, 1 hit
PF02319 E2F_TDP, 1 hit
SMARTiView protein in SMART
SM01372 E2F_TDP, 1 hit
SUPFAMiSSF144074 SSF144074, 1 hit
SSF46785 SSF46785, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9FV71-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEEVPQQFP SSKRQLHPSL SSMKPPLVAP GEYHRFDAAE TRGGGAVADQ
60 70 80 90 100
VVSDAIVIKS TLKRKTDLVN QIVEVNELNT GVLQTPVSGK GGKAKKTSRS
110 120 130 140 150
AKSNKSGTLA SGSNAGSPGN NFAQAGTCRY DSSLGLLTKK FINLIKQAED
160 170 180 190 200
GILDLNKAAD TLEVQKRRIY DITNVLEGIG LIEKTLKNRI QWKGLDVSKP
210 220 230 240 250
GETIESIANL QDEVQNLAAE EARLDDQIRE SQERLTSLSE DENNKRLLFV
260 270 280 290 300
TENDIKNLPC FQNKTLIAVK APHGTTLEVP DPDEAGGYQR RYRIILRSTM
310 320 330 340 350
GPIDVYLVSQ FEESFEDIPQ ADEPSNVPDE PSNVPDVPSN LPSTSGLPEN
360 370 380 390 400
HDVSMPMKEE STERNMETQE VDDTQRVYSD IESHDFVDGI MKIVPPDLDM
410 420 430 440 450
GVDYWFRSEV GEVSITDMWP DESGPDWNQM ITFDQDHAGP SDNKILEQPQ
460
TPSSPTPEES TATRSPTGS
Length:469
Mass (Da):51,653
Last modified:March 1, 2001 - v1
Checksum:iADE1104B34651B7C
GO
Isoform 2 (identifier: Q9FV71-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     423-426: SGPD → Y

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:466
Mass (Da):51,460
Checksum:i8AEE8FFCBA700052
GO

Sequence cautioni

The sequence BAB17029 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti337V → E in CAC15485 (PubMed:11108847).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040804423 – 426SGPD → Y in isoform 2. Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ294533 mRNA Translation: CAC15485.1
AF242580 mRNA Translation: AAG17608.1
AL589883 Genomic DNA Translation: CAC34515.1
AB007651 Genomic DNA Translation: BAB17029.1 Sequence problems.
CP002688 Genomic DNA Translation: AED92998.1
CP002688 Genomic DNA Translation: AED92999.1
AY136479 mRNA Translation: AAM97144.1
BT006610 mRNA Translation: AAP31954.1
RefSeqiNP_001031921.3, NM_001036844.3 [Q9FV71-2]
NP_568413.1, NM_122128.3 [Q9FV71-1]
UniGeneiAt.10189

Genome annotation databases

EnsemblPlantsiAT5G22220.2; AT5G22220.2; AT5G22220 [Q9FV71-1]
AT5G22220.3; AT5G22220.3; AT5G22220 [Q9FV71-2]
GeneIDi832283
GrameneiAT5G22220.2; AT5G22220.2; AT5G22220 [Q9FV71-1]
AT5G22220.3; AT5G22220.3; AT5G22220 [Q9FV71-2]
KEGGiath:AT5G22220

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiE2FB_ARATH
AccessioniPrimary (citable) accession number: Q9FV71
Secondary accession number(s): Q9FMS8, Q9FNY1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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