Q9FV51 (AMP1C_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine aminopeptidase 1C, chloroplastic/mitochondrial
Short name=MAP 1C
Short name=MetAP 1C
EC=3.4.11.18

Alternative name(s):
Peptidase M 1C

Gene names

Name:	MAP1C
Ordered Locus Names:	At3g25740
ORF Names:	K13N2.8

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity.
Subcellular location	Plastid › chloroplast. Mitochondrion Ref.1.
Tissue specificity	Ubiquitous. Ref.1
Sequence similarities	Belongs to the peptidase M24A family.
Sequence caution	The sequence BAA95761.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Chloroplast Mitochondrion Plastid
Domain	Transit peptide
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	N-terminal protein amino acid modification Traceable author statement. Source: TAIR proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Traceable author statement. Source: TAIR mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast and mitochondrion Potential
	Chain	? – 344		Methionine aminopeptidase 1C, chloroplastic/mitochondrial		PRO_0000045806
Sites
	Metal binding	189	1	Cobalt 1 By similarity
	Metal binding	200	1	Cobalt 1 By similarity
	Metal binding	200	1	Cobalt 2 By similarity
	Metal binding	262	1	Cobalt 2 By similarity
	Metal binding	296	1	Cobalt 2 By similarity
	Metal binding	327	1	Cobalt 1 By similarity
	Metal binding	327	1	Cobalt 2 By similarity
	Binding site	172	1	Substrate By similarity
	Binding site	269	1	Substrate By similarity
Experimental info
	Sequence conflict	264	1	V → L in AAG33976. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9FV51 [UniParc]. Last modified January 24, 2006. Version 2. Checksum: ABD84A7FF64D70BA	FASTA	344	37,679
	10 20 30 40 50 60 MLQKISQSIS LCNGDQFKPL IYLAGAPTNF ISSPLSGKKK SSSLRIKRIQ QLQSTLEDRI 70 80 90 100 110 120 NPPLVCGTVS PRLSVPDHIL KPLYVESSKV PEISSELQIP DSIGIVKMKK ACELAARVLD 130 140 150 160 170 180 YAGTLVRPFV TTDEIDKAVH QMVIEFGAYP SPLGYGGFPK SVCTSVNECM FHGIPDSRPL 190 200 210 220 230 240 QNGDIINIDV AVYLDGYHGD TSKTFLCGDV NGSLKQLVKV TEECLEKGIS VCKDGASFKQ 250 260 270 280 290 300 IGKIISEHAA KYGYNMERFI GHGVGTVLHS EPLIYLHSNY DYELEYMIEG QTFTLEPILT 310 320 330 340 IGTTEFVTWP DKWTIVTADG GPAAQFEHTI LITTTGAEIL TISS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of eukaryotic peptide deformylases reveals universality of N-terminal protein processing mechanisms." Giglione C., Serero A., Pierre M., Boisson B., Meinnel T. EMBO J. 19:5916-5929(2000) [PubMed: 11060042] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF250962 mRNA. Translation: AAG33976.1. AB028607 Genomic DNA. Translation: BAA95761.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE77063.1.
IPI	IPI00533123.
RefSeq	NP_189202.1. NM_113473.2.
UniGene	At.16911.
3D structure databases
ProteinModelPortal	Q9FV51.
SMR	Q9FV51. Positions 16-343.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9FV51.
Protein family/group databases
MEROPS	M24.A07.
Proteomic databases
PRIDE	Q9FV51.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G25740.1; AT3G25740.1; AT3G25740.
GeneID	822165.
GenomeReviews	Gene locus AT3G25740 in contig BA000014_GR.
KEGG	ath:AT3G25740.
NMPDR	fig|3702.1.peg.14832.
Organism-specific databases
GeneFarm	2287. 184.
TAIR	At3g25740.
Phylogenomic databases
GeneTree	EPGT00050000011147.
HOGENOM	HBG299384.
InParanoid	Q9FV51.
OMA	MERFIGH.
PhylomeDB	Q9FV51.
ProtClustDB	CLSN2915435.
Gene expression databases
Genevestigator	Q9FV51.
GermOnline	AT3G25740. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
TIGRFAMs	TIGR00500. Met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMP1C_ARATH

Accession

Primary (citable) accession number: Q9FV51
Secondary accession number(s): Q9LS05

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	January 24, 2006
Last modified:	November 16, 2011
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program