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Protein

Methionine aminopeptidase 1D, chloroplastic/mitochondrial

Gene

MAP1D

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei180SubstrateUniRule annotation1
Metal bindingi197Divalent metal cation 1UniRule annotation1
Metal bindingi208Divalent metal cation 1UniRule annotation1
Metal bindingi208Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi271Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei278SubstrateUniRule annotation1
Metal bindingi303Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi334Divalent metal cation 1UniRule annotation1
Metal bindingi334Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • N-terminal protein amino acid modification Source: TAIR
  • response to abscisic acid Source: TAIR

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandMetal-binding

Protein family/group databases

MEROPSiM24.A06

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase 1D, chloroplastic/mitochondrialUniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAP 1DUniRule annotation
Short name:
MetAP 1DUniRule annotation
Alternative name(s):
Peptidase M 1DUniRule annotation
Gene namesi
Name:MAP1D
Ordered Locus Names:At4g37040
ORF Names:C7A10.320
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G37040
TAIRilocus:2115125 AT4G37040

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 49Chloroplast and mitochondrionUniRule annotationCombined sourcesAdd BLAST49
ChainiPRO_000004580750 – 350Methionine aminopeptidase 1D, chloroplastic/mitochondrialAdd BLAST301

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei50N-acetylglycineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9FV50
PRIDEiQ9FV50

PTM databases

iPTMnetiQ9FV50

Expressioni

Tissue specificityi

Ubiquitous. Preferentially expressed in green tissues.1 Publication

Gene expression databases

ExpressionAtlasiQ9FV50 baseline and differential
GenevisibleiQ9FV50 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G37040.1

Structurei

3D structure databases

ProteinModelPortaliQ9FV50
SMRiQ9FV50
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2738 Eukaryota
COG0024 LUCA
HOGENOMiHOG000030427
InParanoidiQ9FV50
KOiK01265
OMAiSYFHGPP
OrthoDBiEOG09360EGZ
PhylomeDBiQ9FV50

Family and domain databases

CDDicd01086 MetAP1, 1 hit
HAMAPiMF_01974 MetAP_1, 1 hit
InterProiView protein in InterPro
IPR036005 Creatinase/aminopeptidase-like
IPR000994 Pept_M24
IPR001714 Pept_M24_MAP
IPR002467 Pept_M24A_MAP1
PfamiView protein in Pfam
PF00557 Peptidase_M24, 1 hit
PRINTSiPR00599 MAPEPTIDASE
SUPFAMiSSF55920 SSF55920, 1 hit
TIGRFAMsiTIGR00500 met_pdase_I, 1 hit
PROSITEiView protein in PROSITE
PS00680 MAP_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FV50-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGVKSLQPR LISSFLGNNS IRSTQPLIHL FRFDLGRRHV SMQLSRTFSG
60 70 80 90 100
LTDLLFNRRN EDEVIDGKRK RLRPGNVSPR RPVPGHITKP PYVDSLQAPG
110 120 130 140 150
ISSGLEVHDK KGIECMRASG ILAARVRDYA GTLVKPGVTT DEIDEAVHNM
160 170 180 190 200
IIENGAYPSP LGYGGFPKSV CTSVNECICH GIPDSRPLED GDIINIDVTV
210 220 230 240 250
YLNGYHGDTS ATFFCGNVDE KAKKLVEVTK ESLDKAISIC GPGVEYKKIG
260 270 280 290 300
KVIHDLADKH KYGVVRQFVG HGVGSVFHAD PVVLHFRNNE AGRMVLNQTF
310 320 330 340 350
TIEPMLTIGS RNPIMWDDNW TVVTEDASLS AQFEHTILIT KDGAEILTKC
Length:350
Mass (Da):38,532
Last modified:March 1, 2001 - v1
Checksum:i0BB60261008FF99A
GO

Sequence cautioni

The sequence CAB16790 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80370 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF250963 mRNA Translation: AAG33977.1
Z99707 Genomic DNA Translation: CAB16790.1 Sequence problems.
AL161590 Genomic DNA Translation: CAB80370.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE86740.1
AK118314 mRNA Translation: BAC42930.1
BT005680 mRNA Translation: AAO64100.1
AY085839 mRNA Translation: AAM63054.1
PIRiE85437
RefSeqiNP_568014.1, NM_119867.3
UniGeneiAt.4646

Genome annotation databases

EnsemblPlantsiAT4G37040.1; AT4G37040.1; AT4G37040
GeneIDi829858
GrameneiAT4G37040.1; AT4G37040.1; AT4G37040
KEGGiath:AT4G37040

Similar proteinsi

Entry informationi

Entry nameiMAP1D_ARATH
AccessioniPrimary (citable) accession number: Q9FV50
Secondary accession number(s): Q9SW64
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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