Q9FUY2 (LEUNG_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional corepressor LEUNIG

Gene names

Name:	LUG
Ordered Locus Names:	At4g32551
ORF Names:	L23H3.30

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	931 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription repressor subunit of the SEU-LUG transcriptional corepressor of the C class floral homeotic gene AGAMOUS during the early stages of floral meristem development. Is part of the A class cadastral complex that define the boundaries between the A and C class homeotic genes expression and function. Interacts together with APETALA2 and SEUSS to repress AGAMOUS expression. Also plays a role in ovule and pollen development. Ref.4 Ref.5 Ref.6
Subunit structure	Forms a corepressor complex with SEU; LUG is the transcription repressor subunit and SEU the specific DNA-binding adapter. Interacts with AGL24-AP1 and SVP-AP1 dimers, and possibly with SEP3, especially when complexed to SEU. Ref.6 Ref.7
Subcellular location	Nucleus Ref.6.
Tissue specificity	Expressed in young flower primordia, later becomes localized to petals, stamens and carpels. Is also expressed in vegetative tissues.
Developmental stage	Expressed prominently during both female and male gametes development.
Disruption phenotype	Ectopic expression of AGAMOUS, leading to the replacement of sepals by carpels and stamens and of petals by stamens. Ref.6
Sequence similarities	Contains 1 LisH domain. Contains 7 WD repeats.
Sequence caution	The sequence CAB43692.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB79972.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Differentiation Flowering Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat WD repeat
Molecular function	Developmental protein Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW flower development Inferred from mutant phenotype Ref.4. Source: TAIR mucilage extrusion from seed coat Inferred from mutant phenotype. Source: TAIR negative regulation of transcription, DNA-dependent Inferred from direct assay Ref.6. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from direct assay Ref.1. Source: TAIR
Molecular function	protein heterodimerization activity Inferred from physical interaction Ref.7. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SEU	Q8W234	6	EBI-1771115,EBI-1771131

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9FUY2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 931	931	Transcriptional corepressor LEUNIG		PRO_0000051069
Regions
	Domain	8 – 40	33	LisH
	Repeat	641 – 679	39	WD 1
	Repeat	683 – 721	39	WD 2
	Repeat	726 – 765	40	WD 3
	Repeat	769 – 804	36	WD 4
	Repeat	808 – 845	38	WD 5
	Repeat	852 – 890	39	WD 6
	Repeat	893 – 931	39	WD 7
	Region	1 – 88	88	Required for SEU-binding
	Compositional bias	89 – 184	96	Gln-rich
	Compositional bias	350 – 385	36	Gln-rich
	Compositional bias	449 – 492	44	Gln-rich
Experimental info
	Sequence conflict	404	1	T → S in AAG32022. Ref.1
	Sequence conflict	454	1	N → H in AAG32022. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 9, 2003. Version 2. Checksum: 7CB879744496A8AA	FASTA	931	102,233
	10 20 30 40 50 60 MSQTNWEADK MLDVYIHDYL VKRDLKATAQ AFQAEGKVSS DPVAIDAPGG FLFEWWSVFW 70 80 90 100 110 120 DIFIARTNEK HSEVAASYIE TQMIKAREQQ LQQSQHPQVS QQQQQQQQQQ IQMQQLLLQR 130 140 150 160 170 180 AQQQQQQQQQ QHHHHQQQQQ QQQQQQQQQQ QQQQQHQNQP PSQQQQQQST PQHQQQPTPQ 190 200 210 220 230 240 QQPQRRDGSH LANGSANGLV GNNSEPVMRQ NPGSGSSLAS KAYEERVKMP TQRESLDEAA 250 260 270 280 290 300 MKRFGDNVGQ LLDPSHASIL KSAAASGQPA GQVLHSTSGG MSPQVQTRNQ QLPGSAVDIK 310 320 330 340 350 360 SEINPVLTPR TAVPEGSLIG IPGSNQGSNN LTLKGWPLTG FDQLRSGLLQ QQKPFMQSQS 370 380 390 400 410 420 FHQLNMLTPQ HQQQLMLAQQ NLNSQSVSEE NRRLKMLLNN RSMTLGKDGL GSSVGDVLPN 430 440 450 460 470 480 VGSSLQPGGS LLPRGDTDML LKLKMALLQQ QQQNQQQGGG NPPQPQPQPQ PLNQLALTNP 490 500 510 520 530 540 QPQSSNHSIH QQEKLGGGGS ITMDGSISNS FRGNEQVLKN QSGRKRKQPV SSSGPANSSG 550 560 570 580 590 600 TANTAGPSPS SAPSTPSTHT PGDVISMPNL PHSGGSSKSM MMFGTEGTGT LTSPSNQLAD 610 620 630 640 650 660 MDRFVEDGSL DDNVESFLSQ EDGDQRDAVT RCMDVSKGFT FTEVNSVRAS TTKVTCCHFS 670 680 690 700 710 720 SDGKMLASAG HDKKAVLWYT DTMKPKTTLE EHTAMITDIR FSPSQLRLAT SSFDKTVRVW 730 740 750 760 770 780 DADNKGYSLR TFMGHSSMVT SLDFHPIKDD LICSCDNDNE IRYWSINNGS CTRVYKGGST 790 800 810 820 830 840 QIRFQPRVGK YLAASSANLV NVLDVETQAI RHSLQGHANP INSVCWDPSG DFLASVSEDM 850 860 870 880 890 900 VKVWTLGTGS EGECVHELSC NGNKFQSCVF HPAYPSLLVI GCYQSLELWN MSENKTMTLP 910 920 930 AHEGLITSLA VSTATGLVAS ASHDKLVKLW K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"LEUNIG, a putative transcriptional corepressor that regulates AGAMOUS expression during flower development." Conner J., Liu Z. Proc. Natl. Acad. Sci. U.S.A. 97:12902-12907(2000) [PubMed: 11058164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Strain: cv. Landsberg erecta.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"LEUNIG regulates AGAMOUS expression in Arabidopsis flowers." Liu Z., Meyerowitz E.M. Development 121:975-991(1995) [PubMed: 7743940] [Abstract] Cited for: FUNCTION.
[5]	"SEUSS, a member of a novel family of plant regulatory proteins, represses floral homeotic gene expression with LEUNIG." Franks R.G., Wang C., Levin J.Z., Liu Z. Development 129:253-263(2002) [PubMed: 11782418] [Abstract] Cited for: FUNCTION.
[6]	"Transcriptional repression of target genes by LEUNIG and SEUSS, two interacting regulatory proteins for Arabidopsis flower development." Sridhar V.V., Surendrarao A., Gonzalez D., Conlan R.S., Liu Z. Proc. Natl. Acad. Sci. U.S.A. 101:11494-11499(2004) [PubMed: 15277686] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, INTERACTION WITH WITH SEU.
[7]	"AGL24, SHORT VEGETATIVE PHASE, and APETALA1 redundantly control AGAMOUS during early stages of flower development in Arabidopsis." Gregis V., Sessa A., Colombo L., Kater M.M. Plant Cell 18:1373-1382(2006) [PubMed: 16679456] [Abstract] Cited for: INTERACTION WITH SEU; AGL24-AP1 AND SVP-AP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF277458 mRNA. Translation: AAG32022.1. AL050398 Genomic DNA. Translation: CAB43692.1. Sequence problems. AL161581 Genomic DNA. Translation: CAB79972.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE86076.1.
IPI	IPI00536685.
PIR	T08588.
RefSeq	NP_567896.1. NM_119407.2.
UniGene	At.22027.
3D structure databases
ProteinModelPortal	Q9FUY2.
SMR	Q9FUY2. Positions 596-931.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9FUY2. 3 interactions.
STRING	Q9FUY2.
Proteomic databases
PRIDE	Q9FUY2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G32551.1; AT4G32551.1; AT4G32551.
GeneID	829390.
GenomeReviews	Gene locus AT4G32551 in contig CT486007_GR.
KEGG	ath:AT4G32551.
NMPDR	fig|3702.1.peg.21280.
Organism-specific databases
GeneFarm	3235. 267.
TAIR	At4g32551.
Phylogenomic databases
eggNOG	KOG0266.
GeneTree	EPGT00050000004467.
HOGENOM	HBG590862.
InParanoid	Q9FUY2.
OMA	MTLPAHE.
PhylomeDB	Q9FUY2.
ProtClustDB	CLSN2691126.
Gene expression databases
ArrayExpress	Q9FUY2.
Genevestigator	Q9FUY2.
GermOnline	AT4G32551. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006594. LisH_dimerisation. IPR013720. LisH_dimerisation_subgr. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 2 hits.
Pfam	PF08513. LisH. 1 hit. PF00400. WD40. 5 hits. [Graphical view]
SMART	SM00667. LisH. 1 hit. SM00320. WD40. 6 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS50896. LISH. 1 hit. PS00678. WD_REPEATS_1. 1 hit. PS50082. WD_REPEATS_2. 5 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEUNG_ARATH

Accession

Primary (citable) accession number: Q9FUY2
Secondary accession number(s): Q9SZY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 9, 2003
Last sequence update:	May 9, 2003
Last modified:	January 25, 2012
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program