Q9FUT0 (GSTU9_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase U9
Short name=AtGSTU9
EC=2.5.1.18

Alternative name(s):
GST class-tau member 9
Glutathione S-transferase 14

Gene names

Name:	GSTU9
Synonyms:	GST14, GST14B
Ordered Locus Names:	At5g62480
ORF Names:	K19B1.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subcellular location	Cytoplasm › cytosol Probable.
Sequence similarities	Belongs to the GST superfamily. Tau family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Biological process	Detoxification
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to toxic substance Inferred from electronic annotation. Source: UniProtKB-KW toxin catabolic process Traceable author statement Ref.1. Source: TAIR
Cellular_component	cytoplasm Non-traceable author statement Ref.1. Source: TAIR cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9FUT0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9FUT0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     65-90: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	Glutathione S-transferase U9		PRO_0000413555
Regions
	Domain	7 – 86	80	GST N-terminal
	Domain	92 – 226	135	GST C-terminal
	Region	17 – 18	2	Glutathione binding By similarity
	Region	43 – 44	2	Glutathione binding By similarity
	Region	57 – 58	2	Glutathione binding By similarity
	Region	70 – 71	2	Glutathione binding By similarity
Natural variations
	Alternative sequence	65 – 90	26	Missing in isoform 2.		VSP_041940

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: D0872464356EA2FB	FASTA	240	27,612
	10 20 30 40 50 60 MDEEVENKVI LHGSFASPYS KRIELALRLK SIPYQFVQED LQNKSQTLLR YNPVHKKIPV 70 80 90 100 110 120 LVHNGKPISE SLFIIEYIDE TWSNGPHILP EDPYRRSKVR FWANYIQLHL YDLVIKVVKS 130 140 150 160 170 180 EGEEQKKALT EVKEKLSVIE KEGLKEIFSD TDGEPTVTNE TMSLVDIVMC TLLSPYKAHE 190 200 210 220 230 240 EVLGLKIIDP EIVPGVYGWI NAINETSVVK DLSPPYEQIL EILRAFRQMS LSPVLETYQS
	Isoform 2 [UniParc]. Checksum: 644A7B5E201D30D3	FASTA	214	24,673
	10 20 30 40 50 60 MDEEVENKVI LHGSFASPYS KRIELALRLK SIPYQFVQED LQNKSQTLLR YNPVHKKIPV 70 80 90 100 110 120 LVHNEDPYRR SKVRFWANYI QLHLYDLVIK VVKSEGEEQK KALTEVKEKL SVIEKEGLKE 130 140 150 160 170 180 IFSDTDGEPT VTNETMSLVD IVMCTLLSPY KAHEEVLGLK IIDPEIVPGV YGWINAINET 190 200 210 SVVKDLSPPY EQILEILRAF RQMSLSPVLE TYQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Probing the diversity of the Arabidopsis glutathione S-transferase gene family." Wagner U., Edwards R., Dixon D.P., Mauch F. Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), GENE FAMILY, NOMENCLATURE. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones." Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S. DNA Res. 5:297-308(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF288179 mRNA. Translation: AAG30128.1. AF288180 mRNA. Translation: AAG30129.1. AB015469 Genomic DNA. Translation: BAB11498.1. CP002688 Genomic DNA. Translation: AED97612.1. CP002688 Genomic DNA. Translation: AED97613.1. AK176211 mRNA. Translation: BAD43974.1.
IPI	IPI00536701.
RefSeq	NP_568954.2. NM_125642.2. NP_851249.1. NM_180918.2.
UniGene	At.9304.
3D structure databases
ProteinModelPortal	Q9FUT0.
SMR	Q9FUT0. Positions 8-228.
ModBase	Search...
Proteomic databases
PRIDE	Q9FUT0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G62480.1; AT5G62480.1; AT5G62480.
GeneID	836368.
KEGG	ath:AT5G62480.
Organism-specific databases
TAIR	At5g62480.
Phylogenomic databases
HOGENOM	HOG000125749.
InParanoid	Q9FUT0.
KO	K00799.
OMA	WINAINE.
PhylomeDB	Q9FUT0.
ProtClustDB	CLSN2680591.
Gene expression databases
ArrayExpress	Q9FUT0.
Genevestigator	Q9FUT0.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTU9_ARATH

Accession

Primary (citable) accession number: Q9FUT0
Secondary accession number(s): Q9FE97

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	March 1, 2001
Last modified:	May 29, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program