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Protein

Glutathione S-transferase U13

Gene

GSTU13

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

In vitro, possesses glutathione S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB) and benzyl isothiocyanate (BITC). May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides.1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

GO - Molecular functioni

  1. glutathione transferase activity Source: UniProtKB-EC

GO - Biological processi

  1. response to cadmium ion Source: TAIR
  2. response to toxic substance Source: UniProtKB-KW
  3. toxin catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Detoxification, Stress response

Enzyme and pathway databases

BioCyciARA:AT1G27130-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase U13 (EC:2.5.1.18)
Short name:
AtGSTU13
Alternative name(s):
GST class-tau member 13
Glutathione S-transferase 12
Gene namesi
Name:GSTU13
Synonyms:GST12
Ordered Locus Names:At1g27130
ORF Names:T7N9.190
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G27130.

Subcellular locationi

  1. Cytoplasmcytosol Curated

GO - Cellular componenti

  1. cytoplasm Source: TAIR
  2. cytosol Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227Glutathione S-transferase U13PRO_0000413559Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei158 – 1581PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9FUS6.

Expressioni

Inductioni

By Hyaloperonospora parasitica. Down-regulated by salicylic acid, ethylene and methyl jasmonate.1 Publication

Gene expression databases

ExpressionAtlasiQ9FUS6. baseline and differential.
GenevestigatoriQ9FUS6.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G27130.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FUS6.
SMRiQ9FUS6. Positions 5-223.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 8682GST N-terminalAdd
BLAST
Domaini92 – 224133GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni15 – 162Glutathione bindingBy similarity
Regioni43 – 442Glutathione bindingBy similarity
Regioni57 – 582Glutathione bindingBy similarity
Regioni70 – 712Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Tau family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

InParanoidiQ9FUS6.
KOiK00799.
OMAiMECLAIL.
PhylomeDBiQ9FUS6.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FUS6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQNDTVKLI GSWSSPYSLR ARVALHLKSV KYEYLDEPDV LKEKSELLLK
60 70 80 90 100
SNPIHKKVPV LLHGDLSISE SLNVVQYVDE AWPSVPSILP SDAYDRASAR
110 120 130 140 150
FWAQYIDDKC FAAVDAVVGA KDDEGKMAAV GKLMECLAIL EETFQKSSKG
160 170 180 190 200
LGFFGGETIG YLDIACSALL GPISVIEAFS GVKFLRQETT PGLIKWAERF
210 220
RAHEAVKPYM PTVEEVVAFA KQKFNVQ
Length:227
Mass (Da):25,131
Last modified:March 1, 2001 - v1
Checksum:i2D0EB66EF50A690E
GO

Sequence cautioni

The sequence AAF79859.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At1g27130 has been split into 2 genes: At1g27130 and At1g27140.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41N → K in AAM64510 (Ref. 5) Curated
Sequence conflicti69 – 691S → C in AAM64510 (Ref. 5) Curated
Sequence conflicti130 – 1301V → A in AAM64510 (Ref. 5) Curated
Sequence conflicti142 – 1421E → D in AAM64510 (Ref. 5) Curated
Sequence conflicti147 – 1471S → T in AAM64510 (Ref. 5) Curated
Sequence conflicti187 – 1871Q → L in AAM64510 (Ref. 5) Curated
Sequence conflicti199 – 1991R → S in AAM64510 (Ref. 5) Curated
Sequence conflicti212 – 2121T → S in AAM64510 (Ref. 5) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF288193 mRNA. Translation: AAG30142.1.
AC000348 Genomic DNA. Translation: AAF79859.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE30784.1.
AY044324 mRNA. Translation: AAK73265.1.
AY050343 mRNA. Translation: AAK91360.1.
AY094051 mRNA. Translation: AAM16207.1.
AY086946 mRNA. Translation: AAM64510.1.
PIRiH86397.
RefSeqiNP_174033.1. NM_102475.2.
UniGeneiAt.16269.

Genome annotation databases

EnsemblPlantsiAT1G27130.1; AT1G27130.1; AT1G27130.
GeneIDi839602.
KEGGiath:AT1G27130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF288193 mRNA. Translation: AAG30142.1.
AC000348 Genomic DNA. Translation: AAF79859.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE30784.1.
AY044324 mRNA. Translation: AAK73265.1.
AY050343 mRNA. Translation: AAK91360.1.
AY094051 mRNA. Translation: AAM16207.1.
AY086946 mRNA. Translation: AAM64510.1.
PIRiH86397.
RefSeqiNP_174033.1. NM_102475.2.
UniGeneiAt.16269.

3D structure databases

ProteinModelPortaliQ9FUS6.
SMRiQ9FUS6. Positions 5-223.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G27130.1-P.

Proteomic databases

PRIDEiQ9FUS6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G27130.1; AT1G27130.1; AT1G27130.
GeneIDi839602.
KEGGiath:AT1G27130.

Organism-specific databases

TAIRiAT1G27130.

Phylogenomic databases

InParanoidiQ9FUS6.
KOiK00799.
OMAiMECLAIL.
PhylomeDBiQ9FUS6.

Enzyme and pathway databases

BioCyciARA:AT1G27130-MONOMER.

Miscellaneous databases

PROiQ9FUS6.

Gene expression databases

ExpressionAtlasiQ9FUS6. baseline and differential.
GenevestigatoriQ9FUS6.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Probing the diversity of the Arabidopsis glutathione S-transferase gene family."
    Wagner U., Edwards R., Dixon D.P., Mauch F.
    Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, GENE FAMILY, NOMENCLATURE.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiGSTUD_ARATH
AccessioniPrimary (citable) accession number: Q9FUS6
Secondary accession number(s): Q8LBX2, Q9LFX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: March 1, 2001
Last modified: April 29, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.