ID   MAN3_SOLLC              Reviewed;         401 AA.
AC   Q9FUQ6;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   27-MAR-2024, entry version 79.
DE   RecName: Full=Mannan endo-1,4-beta-mannosidase 3;
DE            EC=3.2.1.78;
DE   AltName: Full=Beta-mannanase 3;
DE   AltName: Full=Endo-beta-1,4-mannanase 3;
DE   AltName: Full=LeMAN3;
DE   Flags: Precursor;
GN   Name=MAN3;
OS   Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC   Solanum subgen. Lycopersicon.
OX   NCBI_TaxID=4081;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Banik M., Bewley D.;
RT   "Endo-beta-mannanase gene sequence.";
RL   Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC         mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC       {ECO:0000305}.
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DR   EMBL; AF290893; AAG14352.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9FUQ6; -.
DR   SMR; Q9FUQ6; -.
DR   STRING; 4081.Q9FUQ6; -.
DR   CAZy; GH5; Glycoside Hydrolase Family 5.
DR   InParanoid; Q9FUQ6; -.
DR   Proteomes; UP000004994; Unplaced.
DR   ExpressionAtlas; Q9FUQ6; baseline and differential.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IBA:GO_Central.
DR   GO; GO:0071704; P:organic substance metabolic process; IEA:InterPro.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR001547; Glyco_hydro_5.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR045053; MAN-like.
DR   PANTHER; PTHR31451:SF55; MANNAN ENDO-1,4-BETA-MANNOSIDASE 1; 1.
DR   PANTHER; PTHR31451; MANNAN ENDO-1,4-BETA-MANNOSIDASE 7; 1.
DR   Pfam; PF00150; Cellulase; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE   3: Inferred from homology;
KW   Glycosidase; Hydrolase; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..401
FT                   /note="Mannan endo-1,4-beta-mannosidase 3"
FT                   /id="PRO_5000058494"
FT   ACT_SITE        199
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:Q99036"
FT   ACT_SITE        317
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q99036"
FT   BINDING         84
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:B4XC07"
FT   BINDING         198
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:B4XC07"
FT   BINDING         277
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:B4XC07"
FT   BINDING         356
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:B4XC07"
SQ   SEQUENCE   401 AA;  45206 MW;  C1BC41AA2D90A775 CRC64;
     MSYTHRRSCI SGLFLLLLAL SCEANSGFIG VKDSHFELNG SPFLFNGFNS YWLMHVAADP
     AERYKVTEVL KDASTAGLSV CRTWAFSDGG DRALQISPGV YDERVFQGLD FVIAEAKKYG
     VRLILSFVNQ WNDFGGKAEY VWWARNAGVQ ISNDDEFYTH PILKKYLKNH IEVVTRLNSI
     TKVAYKDDAT IMAWELMNEP RDQADYSGKT VNVGWVQEMA SFVKSLDNKH LLEVGMEGFY
     GDSIPERKLV NPGYQVGTDF ISNHLINEID FATIHAYTDQ WLSGQSDEAQ LAWMEKWIRS
     HWEDARNILK KPLVLAEFGK SSRSGEGSRD IFMSSVYRNV YNLAKEGGTM GGSLVWQLMA
     HGMENYDDGY SIVLGLNPST TQIISNQAHI MTALAHSLNH E
//