Reviewed, UniProtKB/Swiss-Prot Q9FUQ6 (MAN3_SOLLC)

Names and origin

Protein names

Recommended name:
    Mannan endo-1,4-beta-mannosidase 3
    EC=3.2.1.78
Alternative name(s):
    Beta-mannanase 3
    Endo-beta-1,4-mannanase 3
    LeMAN3

Gene names

Name:

MAN3

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum)

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 401	377	Mannan endo-1,4-beta-mannosidase 3		PRO_5000058494
Sites
	Active site	199	1	Proton donor By similarity
	Active site	317	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FUQ6-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: C1BC41AA2D90A775	FASTA	401	45,206
	10 20 30 40 50 60 MSYTHRRSCI SGLFLLLLAL SCEANSGFIG VKDSHFELNG SPFLFNGFNS YWLMHVAADP 70 80 90 100 110 120 AERYKVTEVL KDASTAGLSV CRTWAFSDGG DRALQISPGV YDERVFQGLD FVIAEAKKYG 130 140 150 160 170 180 VRLILSFVNQ WNDFGGKAEY VWWARNAGVQ ISNDDEFYTH PILKKYLKNH IEVVTRLNSI 190 200 210 220 230 240 TKVAYKDDAT IMAWELMNEP RDQADYSGKT VNVGWVQEMA SFVKSLDNKH LLEVGMEGFY 250 260 270 280 290 300 GDSIPERKLV NPGYQVGTDF ISNHLINEID FATIHAYTDQ WLSGQSDEAQ LAWMEKWIRS 310 320 330 340 350 360 HWEDARNILK KPLVLAEFGK SSRSGEGSRD IFMSSVYRNV YNLAKEGGTM GGSLVWQLMA 370 380 390 400 HGMENYDDGY SIVLGLNPST TQIISNQAHI MTALAHSLNH E

References

[1]	"Endo-beta-mannanase gene sequence." Banik M., Bewley D. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF290893 Genomic DNA. Translation: AAG14352.1.
3D structure databases
HSSP	HSSP built from PDB template 1QNR based on UniProtKB Q99036.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.1.78. 281054.
Family and domain databases
InterPro	IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAN3_SOLLC

Accession

Primary (citable) accession number: Q9FUQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)