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Protein

Cytokinin dehydrogenase 7

Gene

CKX7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of cytokinins, a family of N(6)-substituted adenine derivatives that are plant hormones, where the substituent is an isopentenyl group.By similarity

Catalytic activityi

N(6)-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101FAD; via carbonyl oxygen1 Publication1
Binding sitei162FAD; via amide nitrogen1 Publication1
Binding sitei167FAD1 Publication1
Binding sitei228FAD; via amide nitrogen and carbonyl oxygen1 Publication1
Binding sitei479FAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi91 – 95FAD1 Publication5
Nucleotide bindingi96 – 97FAD1 Publication2
Nucleotide bindingi173 – 177FAD1 Publication5
Nucleotide bindingi514 – 517FAD1 Publication4

GO - Molecular functioni

GO - Biological processi

  • cytokinin catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciARA:AT5G21482-MONOMER.
BRENDAi1.5.99.12. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytokinin dehydrogenase 7 (EC:1.5.99.12)
Alternative name(s):
Cytokinin oxidase 7
Short name:
AtCKX5
Short name:
AtCKX7
Short name:
CKO7
Gene namesi
Name:CKX7
Synonyms:CKX5
Ordered Locus Names:At5g21482
ORF Names:F13M11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G21482.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001281561 – 524Cytokinin dehydrogenase 7Add BLAST524

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei96Pros-8alpha-FAD histidine1

Proteomic databases

PaxDbiQ9FUJ1.
PRIDEiQ9FUJ1.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G21482.1.

Structurei

Secondary structure

1524
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi37 – 41Combined sources5
Helixi44 – 49Combined sources6
Beta strandi63 – 66Combined sources4
Helixi71 – 83Combined sources13
Beta strandi84 – 86Combined sources3
Beta strandi89 – 95Combined sources7
Beta strandi98 – 100Combined sources3
Beta strandi107 – 111Combined sources5
Helixi112 – 118Combined sources7
Beta strandi120 – 124Combined sources5
Beta strandi127 – 130Combined sources4
Beta strandi132 – 137Combined sources6
Helixi142 – 153Combined sources12
Beta strandi154 – 156Combined sources3
Beta strandi165 – 167Combined sources3
Helixi168 – 172Combined sources5
Helixi181 – 184Combined sources4
Helixi187 – 190Combined sources4
Beta strandi191 – 198Combined sources8
Beta strandi203 – 206Combined sources4
Helixi212 – 218Combined sources7
Beta strandi224 – 237Combined sources14
Beta strandi240 – 250Combined sources11
Helixi252 – 263Combined sources12
Turni267 – 269Combined sources3
Beta strandi272 – 276Combined sources5
Beta strandi279 – 282Combined sources4
Turni285 – 287Combined sources3
Helixi289 – 291Combined sources3
Helixi302 – 304Combined sources3
Beta strandi311 – 322Combined sources12
Helixi328 – 339Combined sources12
Beta strandi349 – 355Combined sources7
Helixi356 – 360Combined sources5
Helixi363 – 373Combined sources11
Beta strandi376 – 378Combined sources3
Beta strandi383 – 388Combined sources6
Helixi389 – 398Combined sources10
Helixi399 – 403Combined sources5
Turni404 – 406Combined sources3
Beta strandi412 – 418Combined sources7
Helixi419 – 421Combined sources3
Beta strandi435 – 442Combined sources8
Helixi452 – 471Combined sources20
Beta strandi476 – 480Combined sources5
Helixi486 – 493Combined sources8
Helixi494 – 496Combined sources3
Helixi497 – 507Combined sources11
Helixi515 – 517Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EXRX-ray1.70A2-524[»]
2Q4WX-ray1.70A2-524[»]
ProteinModelPortaliQ9FUJ1.
SMRiQ9FUJ1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9FUJ1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 238FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST181

Sequence similaritiesi

Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1231. Eukaryota.
COG0277. LUCA.
HOGENOMiHOG000237593.
InParanoidiQ9FUJ1.
KOiK00279.
OMAiPVNGWPT.
OrthoDBiEOG093606YD.
PhylomeDBiQ9FUJ1.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.40.462.10. 1 hit.
InterProiIPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR015345. Cytokinin_DH_FAD/cytokin-bd.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR016164. FAD-linked_Oxase-like_C.
IPR006094. Oxid_FAD_bind_N.
IPR016170. V_Alc_oxidase/Cytok_DH_C_dom.
[Graphical view]
PfamiPF09265. Cytokin-bind. 1 hit.
PF01565. FAD_binding_4. 1 hit.
[Graphical view]
SUPFAMiSSF55103. SSF55103. 1 hit.
SSF56176. SSF56176. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FUJ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIAYIEPYFL ENDAEAASAA TAAGKSTDGV SESLNIQGEI LCGGAAADIA
60 70 80 90 100
GRDFGGMNCV KPLAVVRPVG PEDIAGAVKA ALRSDKLTVA ARGNGHSING
110 120 130 140 150
QAMAEGGLVV DMSTTAENHF EVGYLSGGDA TAFVDVSGGA LWEDVLKRCV
160 170 180 190 200
SEYGLAPRSW TDYLGLTVGG TLSNAGVSGQ AFRYGPQTSN VTELDVVTGN
210 220 230 240 250
GDVVTCSEIE NSELFFSVLG GLGQFGIITR ARVLLQPAPD MVRWIRVVYT
260 270 280 290 300
EFDEFTQDAE WLVSQKNESS FDYVEGFVFV NGADPVNGWP TVPLHPDHEF
310 320 330 340 350
DPTRLPQSCG SVLYCLELGL HYRDSDSNST IDKRVERLIG RLRFNEGLRF
360 370 380 390 400
EVDLPYVDFL LRVKRSEEIA KENGTWETPH PWLNLFVSKR DIGDFNRTVF
410 420 430 440 450
KELVKNGVNG PMLVYPLLRS RWDDRTSVVI PEEGEIFYIV ALLRFVPPCA
460 470 480 490 500
KVSSVEKMVA QNQEIVHWCV KNGIDYKLYL PHYKSQEEWI RHFGNRWSRF
510 520
VDRKAMFDPM AILSPGQKIF NRSL
Length:524
Mass (Da):57,976
Last modified:March 1, 2001 - v1
Checksum:i9ADB3BE97A7F063C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF303981 mRNA. Translation: AAG30908.1.
AC140977 Genomic DNA. Translation: AAO73882.1.
CP002688 Genomic DNA. Translation: AED92951.1.
RefSeqiNP_850863.1. NM_180532.3.
UniGeneiAt.16886.

Genome annotation databases

EnsemblPlantsiAT5G21482.1; AT5G21482.1; AT5G21482.
GeneIDi832248.
GrameneiAT5G21482.1; AT5G21482.1; AT5G21482.
KEGGiath:AT5G21482.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF303981 mRNA. Translation: AAG30908.1.
AC140977 Genomic DNA. Translation: AAO73882.1.
CP002688 Genomic DNA. Translation: AED92951.1.
RefSeqiNP_850863.1. NM_180532.3.
UniGeneiAt.16886.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EXRX-ray1.70A2-524[»]
2Q4WX-ray1.70A2-524[»]
ProteinModelPortaliQ9FUJ1.
SMRiQ9FUJ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G21482.1.

Proteomic databases

PaxDbiQ9FUJ1.
PRIDEiQ9FUJ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G21482.1; AT5G21482.1; AT5G21482.
GeneIDi832248.
GrameneiAT5G21482.1; AT5G21482.1; AT5G21482.
KEGGiath:AT5G21482.

Organism-specific databases

TAIRiAT5G21482.

Phylogenomic databases

eggNOGiKOG1231. Eukaryota.
COG0277. LUCA.
HOGENOMiHOG000237593.
InParanoidiQ9FUJ1.
KOiK00279.
OMAiPVNGWPT.
OrthoDBiEOG093606YD.
PhylomeDBiQ9FUJ1.

Enzyme and pathway databases

BioCyciARA:AT5G21482-MONOMER.
BRENDAi1.5.99.12. 399.

Miscellaneous databases

EvolutionaryTraceiQ9FUJ1.
PROiQ9FUJ1.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.40.462.10. 1 hit.
InterProiIPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR015345. Cytokinin_DH_FAD/cytokin-bd.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR016164. FAD-linked_Oxase-like_C.
IPR006094. Oxid_FAD_bind_N.
IPR016170. V_Alc_oxidase/Cytok_DH_C_dom.
[Graphical view]
PfamiPF09265. Cytokin-bind. 1 hit.
PF01565. FAD_binding_4. 1 hit.
[Graphical view]
SUPFAMiSSF55103. SSF55103. 1 hit.
SSF56176. SSF56176. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCKX7_ARATH
AccessioniPrimary (citable) accession number: Q9FUJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.