ID   CHSY_HYPAN              Reviewed;         390 AA.
AC   Q9FUB7;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   27-MAR-2024, entry version 71.
DE   RecName: Full=Chalcone synthase;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase;
OS   Hypericum androsaemum (Tutsan).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Malpighiales; Hypericaceae; Hypericeae; Hypericum.
OX   NCBI_TaxID=140968;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAG30295.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL
RP   PROPERTIES, AND MUTAGENESIS OF GLY-256; LEU-263; PHE-265 AND SER-338.
RX   PubMed=12795704; DOI=10.1046/j.1365-313x.2003.01771.x;
RA   Liu B., Falkenstein-Paul H., Schmidt W., Beerhues L.;
RT   "Benzophenone synthase and chalcone synthase from Hypericum androsaemum
RT   cell cultures: cDNA cloning, functional expression, and site-directed
RT   mutagenesis of two polyketide synthases.";
RL   Plant J. 34:847-855(2003).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which undergoes enzyme-catalyzed or spontaneous isomerization into
CC       naringenin. {ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023,
CC         ECO:0000269|PubMed:12795704};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=10.8 uM for malonyl-CoA {ECO:0000269|PubMed:12795704};
CC         KM=4.9 uM for 4-coumaroyl-CoA {ECO:0000269|PubMed:12795704};
CC         KM=6.8 uM for benzoyl-CoA {ECO:0000269|PubMed:12795704};
CC       pH dependence:
CC         Optimum pH is 7.0. {ECO:0000269|PubMed:12795704};
CC       Temperature dependence:
CC         Optimum temperature is 35 degrees Celsius.
CC         {ECO:0000269|PubMed:12795704};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000269|PubMed:12795704}.
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DR   EMBL; AF315345; AAG30295.1; -; mRNA.
DR   AlphaFoldDB; Q9FUB7; -.
DR   SMR; Q9FUB7; -.
DR   BRENDA; 2.3.1.74; 2742.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IDA:UniProtKB.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IDA:UniProtKB.
DR   CDD; cd00831; CHS_like; 1.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877:SF14; CHALCONE SYNTHASE; 1.
DR   PANTHER; PTHR11877; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; Thiolase-like; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   1: Evidence at protein level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..390
FT                   /note="Chalcone synthase"
FT                   /id="PRO_0000215982"
FT   ACT_SITE        164
FT                   /evidence="ECO:0000250|UniProtKB:P30074,
FT                   ECO:0000255|PROSITE-ProRule:PRU10023"
FT   MUTAGEN         256
FT                   /note="G->A: 63% active."
FT                   /evidence="ECO:0000269|PubMed:12795704"
FT   MUTAGEN         263
FT                   /note="L->M: No effect."
FT                   /evidence="ECO:0000269|PubMed:12795704"
FT   MUTAGEN         265
FT                   /note="F->Y: 67% active."
FT                   /evidence="ECO:0000269|PubMed:12795704"
FT   MUTAGEN         338
FT                   /note="S->G: No effect."
FT                   /evidence="ECO:0000269|PubMed:12795704"
SQ   SEQUENCE   390 AA;  42713 MW;  023326D7DFC44327 CRC64;
     MVTVEEVRKA QRAEGPATVM AIGTAVPPNC VDQATYPDYY FRITNSEHKA ELKEKFQRMC
     DKSQIKKRYM YLNEEVLKEN PNMCAYMAPS LDARQDIVVV EVPKLGKEAA VKAIKEWGQP
     KSKITHLVFC TTSGVDMPGA DYQLTKLLGL RPSVKRLMMY QQGCFAGGTV LRLAKDLAEN
     NKGARVLVVC SEITAVTFRG PTDTHLDSLV GQALFGDGAA AIIIGSDPIP EVEKPLFELV
     SAAQTILPDS EGAIDGHLRE VGLTFHLLKD VPGLISKNVE KSLTEAFKPL GISDWNSLFW
     IAHPGGPAIL DQVEAKLSLK PEKLRATRHV LSEYGNMSSA CVLFILDEMR RKSKEDGLKT
     TGEGIEWGVL FGFGPGLTVE TVVLHSVAIN
//