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Q9FUB7

- CHSY_HYPAN

UniProt

Q9FUB7 - CHSY_HYPAN

Protein

Chalcone synthase

Gene
N/A
Organism
Hypericum androsaemum (Tutsan)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which undergoes enzyme-catalyzed or spontaneous isomerization into naringenin.Curated

    Catalytic activityi

    3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.1 PublicationPROSITE-ProRule annotation

    Kineticsi

    1. KM=10.8 µM for malonyl-CoA1 Publication
    2. KM=4.9 µM for 4-coumaroyl-CoA1 Publication
    3. KM=6.8 µM for benzoyl-CoA1 Publication

    pH dependencei

    Optimum pH is 7.0.1 Publication

    Temperature dependencei

    Optimum temperature is 35 degrees Celsius.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei164 – 1641By similarityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. naringenin-chalcone synthase activity Source: UniProtKB

    GO - Biological processi

    1. flavonoid biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Flavonoid biosynthesis

    Enzyme and pathway databases

    BRENDAi2.3.1.74. 2742.
    UniPathwayiUPA00154.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chalcone synthase (EC:2.3.1.74)
    Alternative name(s):
    Naringenin-chalcone synthase
    OrganismiHypericum androsaemum (Tutsan)
    Taxonomic identifieri140968 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesHypericaceaeHypericeaeHypericum

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi256 – 2561G → A: 63% active. 1 Publication
    Mutagenesisi263 – 2631L → M: No effect. 1 Publication
    Mutagenesisi265 – 2651F → Y: 67% active. 1 Publication
    Mutagenesisi338 – 3381S → G: No effect. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 390390Chalcone synthasePRO_0000215982Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FUB7.
    SMRiQ9FUB7. Positions 3-389.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the chalcone/stilbene synthases family.1 Publication

    Family and domain databases

    Gene3Di3.40.47.10. 2 hits.
    InterProiIPR012328. Chalcone/stilbene_synth_C.
    IPR018088. Chalcone/stilbene_synthase_AS.
    IPR001099. Chalcone/stilbene_synthase_N.
    IPR011141. Polyketide_synthase_type-III.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF02797. Chal_sti_synt_C. 1 hit.
    PF00195. Chal_sti_synt_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000451. PKS_III. 1 hit.
    SUPFAMiSSF53901. SSF53901. 2 hits.
    PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9FUB7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVTVEEVRKA QRAEGPATVM AIGTAVPPNC VDQATYPDYY FRITNSEHKA    50
    ELKEKFQRMC DKSQIKKRYM YLNEEVLKEN PNMCAYMAPS LDARQDIVVV 100
    EVPKLGKEAA VKAIKEWGQP KSKITHLVFC TTSGVDMPGA DYQLTKLLGL 150
    RPSVKRLMMY QQGCFAGGTV LRLAKDLAEN NKGARVLVVC SEITAVTFRG 200
    PTDTHLDSLV GQALFGDGAA AIIIGSDPIP EVEKPLFELV SAAQTILPDS 250
    EGAIDGHLRE VGLTFHLLKD VPGLISKNVE KSLTEAFKPL GISDWNSLFW 300
    IAHPGGPAIL DQVEAKLSLK PEKLRATRHV LSEYGNMSSA CVLFILDEMR 350
    RKSKEDGLKT TGEGIEWGVL FGFGPGLTVE TVVLHSVAIN 390
    Length:390
    Mass (Da):42,713
    Last modified:March 1, 2001 - v1
    Checksum:i023326D7DFC44327
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF315345 mRNA. Translation: AAG30295.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF315345 mRNA. Translation: AAG30295.1 .

    3D structure databases

    ProteinModelPortali Q9FUB7.
    SMRi Q9FUB7. Positions 3-389.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00154 .
    BRENDAi 2.3.1.74. 2742.

    Family and domain databases

    Gene3Di 3.40.47.10. 2 hits.
    InterProi IPR012328. Chalcone/stilbene_synth_C.
    IPR018088. Chalcone/stilbene_synthase_AS.
    IPR001099. Chalcone/stilbene_synthase_N.
    IPR011141. Polyketide_synthase_type-III.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF02797. Chal_sti_synt_C. 1 hit.
    PF00195. Chal_sti_synt_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000451. PKS_III. 1 hit.
    SUPFAMi SSF53901. SSF53901. 2 hits.
    PROSITEi PS00441. CHALCONE_SYNTH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Benzophenone synthase and chalcone synthase from Hypericum androsaemum cell cultures: cDNA cloning, functional expression, and site-directed mutagenesis of two polyketide synthases."
      Liu B., Falkenstein-Paul H., Schmidt W., Beerhues L.
      Plant J. 34:847-855(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF GLY-256; LEU-263; PHE-265 AND SER-338.

    Entry informationi

    Entry nameiCHSY_HYPAN
    AccessioniPrimary (citable) accession number: Q9FUB7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3