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Q9FUB7

- CHSY_HYPAN

UniProt

Q9FUB7 - CHSY_HYPAN

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Protein

Chalcone synthase

Gene
N/A
Organism
Hypericum androsaemum (Tutsan)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which undergoes enzyme-catalyzed or spontaneous isomerization into naringenin.

Catalytic activityi

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.1 Publication

Kineticsi

  1. KM=10.8 µM for malonyl-CoA1 Publication
  2. KM=4.9 µM for 4-coumaroyl-CoA1 Publication
  3. KM=6.8 µM for benzoyl-CoA1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Temperature dependencei

Optimum temperature is 35 degrees Celsius.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei164 – 1641 By similarityBy similarity

GO - Molecular functioni

  1. naringenin-chalcone synthase activity Source: UniProtKB

GO - Biological processi

  1. flavonoid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Flavonoid biosynthesis

Enzyme and pathway databases

BRENDAi2.3.1.74. 2742.
UniPathwayiUPA00154.

Names & Taxonomyi

Protein namesi
Recommended name:
Chalcone synthase (EC:2.3.1.74)
Alternative name(s):
Naringenin-chalcone synthase
OrganismiHypericum androsaemum (Tutsan)
Taxonomic identifieri140968 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesHypericaceaeHypericeaeHypericum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi256 – 2561G → A: 63% active. 1 Publication
Mutagenesisi263 – 2631L → M: No effect. 1 Publication
Mutagenesisi265 – 2651F → Y: 67% active. 1 Publication
Mutagenesisi338 – 3381S → G: No effect. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390Chalcone synthasePRO_0000215982Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ9FUB7.
SMRiQ9FUB7. Positions 3-389.

Family & Domainsi

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.1 Publication

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FUB7-1 [UniParc]FASTAAdd to Basket

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MVTVEEVRKA QRAEGPATVM AIGTAVPPNC VDQATYPDYY FRITNSEHKA    50
ELKEKFQRMC DKSQIKKRYM YLNEEVLKEN PNMCAYMAPS LDARQDIVVV 100
EVPKLGKEAA VKAIKEWGQP KSKITHLVFC TTSGVDMPGA DYQLTKLLGL 150
RPSVKRLMMY QQGCFAGGTV LRLAKDLAEN NKGARVLVVC SEITAVTFRG 200
PTDTHLDSLV GQALFGDGAA AIIIGSDPIP EVEKPLFELV SAAQTILPDS 250
EGAIDGHLRE VGLTFHLLKD VPGLISKNVE KSLTEAFKPL GISDWNSLFW 300
IAHPGGPAIL DQVEAKLSLK PEKLRATRHV LSEYGNMSSA CVLFILDEMR 350
RKSKEDGLKT TGEGIEWGVL FGFGPGLTVE TVVLHSVAIN 390
Length:390
Mass (Da):42,713
Last modified:March 1, 2001 - v1
Checksum:i023326D7DFC44327
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF315345 mRNA. Translation: AAG30295.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF315345 mRNA. Translation: AAG30295.1 .

3D structure databases

ProteinModelPortali Q9FUB7.
SMRi Q9FUB7. Positions 3-389.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00154 .
BRENDAi 2.3.1.74. 2742.

Family and domain databases

Gene3Di 3.40.47.10. 2 hits.
InterProi IPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000451. PKS_III. 1 hit.
SUPFAMi SSF53901. SSF53901. 2 hits.
PROSITEi PS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Benzophenone synthase and chalcone synthase from Hypericum androsaemum cell cultures: cDNA cloning, functional expression, and site-directed mutagenesis of two polyketide synthases."
    Liu B., Falkenstein-Paul H., Schmidt W., Beerhues L.
    Plant J. 34:847-855(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF GLY-256; LEU-263; PHE-265 AND SER-338.

Entry informationi

Entry nameiCHSY_HYPAN
AccessioniPrimary (citable) accession number: Q9FUB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: March 1, 2001
Last modified: February 19, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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