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Protein

Chalcone synthase

Gene
N/A
Organism
Hypericum androsaemum (Tutsan)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which undergoes enzyme-catalyzed or spontaneous isomerization into naringenin.Curated

Catalytic activityi

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.PROSITE-ProRule annotation1 Publication

Kineticsi

  1. KM=10.8 µM for malonyl-CoA1 Publication
  2. KM=4.9 µM for 4-coumaroyl-CoA1 Publication
  3. KM=6.8 µM for benzoyl-CoA1 Publication

    pH dependencei

    Optimum pH is 7.0.1 Publication

    Temperature dependencei

    Optimum temperature is 35 degrees Celsius.1 Publication

    Pathway: flavonoid biosynthesis

    This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei164 – 1641PROSITE-ProRule annotationBy similarity

    GO - Molecular functioni

    • naringenin-chalcone synthase activity Source: UniProtKB

    GO - Biological processi

    • flavonoid biosynthetic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Flavonoid biosynthesis

    Enzyme and pathway databases

    BRENDAi2.3.1.74. 2742.
    UniPathwayiUPA00154.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chalcone synthase (EC:2.3.1.74)
    Alternative name(s):
    Naringenin-chalcone synthase
    OrganismiHypericum androsaemum (Tutsan)
    Taxonomic identifieri140968 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesHypericaceaeHypericeaeHypericum

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi256 – 2561G → A: 63% active. 1 Publication
    Mutagenesisi263 – 2631L → M: No effect. 1 Publication
    Mutagenesisi265 – 2651F → Y: 67% active. 1 Publication
    Mutagenesisi338 – 3381S → G: No effect. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 390390Chalcone synthasePRO_0000215982Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FUB7.
    SMRiQ9FUB7. Positions 3-389.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the chalcone/stilbene synthases family.1 Publication

    Family and domain databases

    Gene3Di3.40.47.10. 2 hits.
    InterProiIPR012328. Chalcone/stilbene_synth_C.
    IPR018088. Chalcone/stilbene_synthase_AS.
    IPR001099. Chalcone/stilbene_synthase_N.
    IPR011141. Polyketide_synthase_type-III.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF02797. Chal_sti_synt_C. 1 hit.
    PF00195. Chal_sti_synt_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000451. PKS_III. 1 hit.
    SUPFAMiSSF53901. SSF53901. 2 hits.
    PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9FUB7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVTVEEVRKA QRAEGPATVM AIGTAVPPNC VDQATYPDYY FRITNSEHKA
    60 70 80 90 100
    ELKEKFQRMC DKSQIKKRYM YLNEEVLKEN PNMCAYMAPS LDARQDIVVV
    110 120 130 140 150
    EVPKLGKEAA VKAIKEWGQP KSKITHLVFC TTSGVDMPGA DYQLTKLLGL
    160 170 180 190 200
    RPSVKRLMMY QQGCFAGGTV LRLAKDLAEN NKGARVLVVC SEITAVTFRG
    210 220 230 240 250
    PTDTHLDSLV GQALFGDGAA AIIIGSDPIP EVEKPLFELV SAAQTILPDS
    260 270 280 290 300
    EGAIDGHLRE VGLTFHLLKD VPGLISKNVE KSLTEAFKPL GISDWNSLFW
    310 320 330 340 350
    IAHPGGPAIL DQVEAKLSLK PEKLRATRHV LSEYGNMSSA CVLFILDEMR
    360 370 380 390
    RKSKEDGLKT TGEGIEWGVL FGFGPGLTVE TVVLHSVAIN
    Length:390
    Mass (Da):42,713
    Last modified:March 1, 2001 - v1
    Checksum:i023326D7DFC44327
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF315345 mRNA. Translation: AAG30295.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF315345 mRNA. Translation: AAG30295.1.

    3D structure databases

    ProteinModelPortaliQ9FUB7.
    SMRiQ9FUB7. Positions 3-389.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    UniPathwayiUPA00154.
    BRENDAi2.3.1.74. 2742.

    Family and domain databases

    Gene3Di3.40.47.10. 2 hits.
    InterProiIPR012328. Chalcone/stilbene_synth_C.
    IPR018088. Chalcone/stilbene_synthase_AS.
    IPR001099. Chalcone/stilbene_synthase_N.
    IPR011141. Polyketide_synthase_type-III.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF02797. Chal_sti_synt_C. 1 hit.
    PF00195. Chal_sti_synt_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000451. PKS_III. 1 hit.
    SUPFAMiSSF53901. SSF53901. 2 hits.
    PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    1. "Benzophenone synthase and chalcone synthase from Hypericum androsaemum cell cultures: cDNA cloning, functional expression, and site-directed mutagenesis of two polyketide synthases."
      Liu B., Falkenstein-Paul H., Schmidt W., Beerhues L.
      Plant J. 34:847-855(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF GLY-256; LEU-263; PHE-265 AND SER-338.

    Entry informationi

    Entry nameiCHSY_HYPAN
    AccessioniPrimary (citable) accession number: Q9FUB7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.