Reviewed, UniProtKB/Swiss-Prot Q9FTZ2 (EBP_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
    EC=5.3.3.5
Alternative name(s):
    Cholestenol Delta-isomerase
    Delta(8)-Delta(7) sterol isomerase
      Short name=D8-D7 sterol isomerase

Gene names

Ordered Locus Names:	Os01g0103600, LOC_Os01g01369
ORF Names:	P0436E04.5, P0455C04.26

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the conversion of Delta(8)-sterols to their corresponding Delta(7)-isomers By similarity.
Catalytic activity	5-alpha-cholest-7-en-3-beta-ol = 5-alpha-cholest-8-en-3-beta-ol.
Pathway	Steroid biosynthesis; sterol biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the EBP family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cholestenol delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9FTZ2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9FTZ2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     137-204: AIASGKSYSH...IPTMIAIRSW → SSFLPFHLTM...ILLFFFPLSS

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase		PRO_0000174346
Regions
	Transmembrane	29 – 49	21	Potential
	Transmembrane	62 – 82	21	Potential
	Transmembrane	119 – 139	21	Potential
	Transmembrane	181 – 201	21	Potential
Natural variations
	Alternative sequence	137 – 204	68	AIASG…AIRSW → SSFLPFHLTMRTLPLSLSLS LSLSLSLSLSLSLSFPFLTY LTIFSLQMHFSHVFLPFVIL LFFFPLSS in isoform 2.		VSP_017657

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 5D4A60D361FCA071	FASTA	219	24,758
	10 20 30 40 50 60 MGHPHPHPYA PAELHLPGFV PLQLSQAQIL VPYLATSLFL LLAVWLISGR CSRRLSDTDR 70 80 90 100 110 120 WLMCWWAFTG LTHIIIEGTF VFAPNFFSNQ NPSYFDEVWK EYSKGDSRYV ARDPATVTVE 130 140 150 160 170 180 GITAVLEGPA SLLAVYAIAS GKSYSHILQF TVCLGQLYGC LVYFITAYLD GFNFWTSPFY 190 200 210 FWAYFIGANS SWVVIPTMIA IRSWKKICAA FQGEKVKTK
	Isoform 2. Checksum: 73EC5ACE3DBC1428	FASTA	219	24,682
	10 20 30 40 50 60 MGHPHPHPYA PAELHLPGFV PLQLSQAQIL VPYLATSLFL LLAVWLISGR CSRRLSDTDR 70 80 90 100 110 120 WLMCWWAFTG LTHIIIEGTF VFAPNFFSNQ NPSYFDEVWK EYSKGDSRYV ARDPATVTVE 130 140 150 160 170 180 GITAVLEGPA SLLAVYSSFL PFHLTMRTLP LSLSLSLSLS LSLSLSLSLS FPFLTYLTIF 190 200 210 SLQMHFSHVF LPFVILLFFF PLSSKKICAA FQGEKVKTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP002818 Genomic DNA. Translation: BAB16323.1. AP002969 Genomic DNA. Translation: BAB92148.1. AP008207 Genomic DNA. Translation: BAF03671.1. AK059848 mRNA. No translation available. AK102848 mRNA. No translation available.
RefSeq	NP_001041757.1.
UniGene	Os.91508
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4326449.
KEGG	osa:4326449.
NMPDR	fig|39947.1.peg.764.
Organism-specific databases
Gramene	Q9FTZ2.
Phylogenomic databases
HOGENOM	HBG590959.
Family and domain databases
InterPro	IPR007905. EBP. [Graphical view]
PANTHER	PTHR14207. EBP. 1 hit.
Pfam	PF05241. EBP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EBP_ORYSJ

Accession

Primary (citable) accession number: Q9FTZ2
Secondary accession number(s): Q0JRF7, Q7F734

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	March 1, 2001
Last modified:	December 15, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)