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Protein

Alpha-galactosidase 1

Gene

AGAL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

May regulate leaf (and possibly other organ) development by functioning in cell wall loosening and cell wall expansion.By similarity

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei173SubstrateBy similarity1
Active sitei175NucleophileBy similarity1
Binding sitei226SubstrateBy similarity1
Active sitei230Proton donorBy similarity1
Binding sitei230SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT5G08380-MONOMER
ReactomeiR-ATH-1660662 Glycosphingolipid metabolism
R-ATH-6798695 Neutrophil degranulation

Protein family/group databases

CAZyiGH27 Glycoside Hydrolase Family 27

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidase 11 Publication (EC:3.2.1.22By similarity)
Short name:
AtAGAL11 Publication
Alternative name(s):
Alpha-D-galactoside galactohydrolase 1Curated
Melibiase 1Curated
Gene namesi
Name:AGAL11 Publication
Ordered Locus Names:At5g08380Imported
ORF Names:F8L15.110Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G08380
TAIRilocus:2150778 AT5G08380

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000043184528 – 410Alpha-galactosidase 1Sequence analysisAdd BLAST383

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi66 ↔ 98By similarity
Disulfide bondi146 ↔ 177By similarity
Glycosylationi297N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi391N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9FT97
PRIDEiQ9FT97
ProMEXiQ9FT97

Expressioni

Gene expression databases

ExpressionAtlasiQ9FT97 baseline and differential
GenevisibleiQ9FT97 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G08380.1

Structurei

3D structure databases

ProteinModelPortaliQ9FT97
SMRiQ9FT97
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni96 – 97Substrate bindingBy similarity2
Regioni208 – 212Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2366 Eukaryota
ENOG410XPF1 LUCA
HOGENOMiHOG000161224
KOiK07407
OMAiLAMTPTM
OrthoDBiEOG09360A7S
PhylomeDBiQ9FT97

Family and domain databases

CDDicd14792 GH27, 1 hit
Gene3Di2.60.40.1180, 1 hit
3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR002241 Glyco_hydro_27
IPR000111 Glyco_hydro_27/36_CS
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF16499 Melibiase_2, 1 hit
PRINTSiPR00740 GLHYDRLASE27
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00512 ALPHA_GALACTOSIDASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FT97-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRAMVIKM PILMILISSM VMTMVESSRS VNNGHDDSEI LRRHLLTNGL
60 70 80 90 100
GVTPPMGWNS WNHFSCNIDE KMIKETADAL VTTGLSKLGY NYVNIDDCWA
110 120 130 140 150
EISRDSKGSL VPKKSTFPSG IKAVADYVHS KGLKLGIYSD AGYFTCSKTM
160 170 180 190 200
PGSLGYEEHD AKTFAEWGID YLKYDNCNSD GSKPTVRYPV MTRALMKSGR
210 220 230 240 250
PIFHSLCEWG DMHPALWGSP VGNSWRTTND IKDTWLSMIS IADMNEVYAE
260 270 280 290 300
HARPGGWNDP DMLEVGNGGM TKDEYIVHFS IWAISKAPLL LGCDIRNMTK
310 320 330 340 350
ETMEIVANKE VIAINQDPHG VQAKKVRMEG DLEVWAGPLS GYRVALLLLN
360 370 380 390 400
RGPSRTSITA LWEDIEIPAN SIVEARDLWE HQTLKQKFVG NLTATVDSHA
410
CKLYVLKPVA
Length:410
Mass (Da):45,709
Last modified:March 1, 2001 - v1
Checksum:iF68C52E35FC11F0A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78D → N in AAM13199 (PubMed:14593172).Curated1
Sequence conflicti78D → N in AAP37856 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL392174 Genomic DNA Translation: CAC08338.1
CP002688 Genomic DNA Translation: AED91292.1
AY093200 mRNA Translation: AAM13199.1
BT008497 mRNA Translation: AAP37856.1
RefSeqiNP_196455.1, NM_120922.5
UniGeneiAt.10139

Genome annotation databases

EnsemblPlantsiAT5G08380.1; AT5G08380.1; AT5G08380
GeneIDi830736
GrameneiAT5G08380.1; AT5G08380.1; AT5G08380
KEGGiath:AT5G08380

Similar proteinsi

Entry informationi

Entry nameiAGAL1_ARATH
AccessioniPrimary (citable) accession number: Q9FT97
Secondary accession number(s): Q8RWB9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 4, 2015
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health