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Protein

Alpha-galactosidase 1

Gene

AGAL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May regulate leaf (and possibly other organ) development by functioning in cell wall loosening and cell wall expansion.By similarity

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei173SubstrateBy similarity1
Active sitei175NucleophileBy similarity1
Binding sitei226SubstrateBy similarity1
Active sitei230Proton donorBy similarity1
Binding sitei230SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT5G08380-MONOMER.
ReactomeiR-ATH-1660662. Glycosphingolipid metabolism.
R-ATH-6798695. Neutrophil degranulation.

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidase 11 Publication (EC:3.2.1.22By similarity)
Short name:
AtAGAL11 Publication
Alternative name(s):
Alpha-D-galactoside galactohydrolase 1Curated
Melibiase 1Curated
Gene namesi
Name:AGAL11 Publication
Ordered Locus Names:At5g08380Imported
ORF Names:F8L15.110Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G08380.

Subcellular locationi

  • Secretedcell wall By similarity
  • Secretedextracellular spaceapoplast By similarity

GO - Cellular componenti

  • apoplast Source: TAIR
  • cell wall Source: TAIR
  • plant-type cell wall Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000043184528 – 410Alpha-galactosidase 1Sequence analysisAdd BLAST383

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi66 ↔ 98By similarity
Disulfide bondi146 ↔ 177By similarity
Glycosylationi297N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi391N-linked (GlcNAc...)PROSITE-ProRule annotation1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9FT97.
PRIDEiQ9FT97.
ProMEXiQ9FT97.

Expressioni

Gene expression databases

GenevisibleiQ9FT97. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G08380.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FT97.
SMRiQ9FT97.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni96 – 97Substrate bindingBy similarity2
Regioni208 – 212Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2366. Eukaryota.
ENOG410XPF1. LUCA.
HOGENOMiHOG000161224.
KOiK07407.
OMAiVAPHSCR.
OrthoDBiEOG09360A7S.
PhylomeDBiQ9FT97.

Family and domain databases

CDDicd14792. GH27. 1 hit.
Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_27/36_CS.
IPR013780. Glyco_hydro_b.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF16499. Melibiase_2. 1 hit.
[Graphical view]
PRINTSiPR00740. GLHYDRLASE27.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FT97-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRAMVIKM PILMILISSM VMTMVESSRS VNNGHDDSEI LRRHLLTNGL
60 70 80 90 100
GVTPPMGWNS WNHFSCNIDE KMIKETADAL VTTGLSKLGY NYVNIDDCWA
110 120 130 140 150
EISRDSKGSL VPKKSTFPSG IKAVADYVHS KGLKLGIYSD AGYFTCSKTM
160 170 180 190 200
PGSLGYEEHD AKTFAEWGID YLKYDNCNSD GSKPTVRYPV MTRALMKSGR
210 220 230 240 250
PIFHSLCEWG DMHPALWGSP VGNSWRTTND IKDTWLSMIS IADMNEVYAE
260 270 280 290 300
HARPGGWNDP DMLEVGNGGM TKDEYIVHFS IWAISKAPLL LGCDIRNMTK
310 320 330 340 350
ETMEIVANKE VIAINQDPHG VQAKKVRMEG DLEVWAGPLS GYRVALLLLN
360 370 380 390 400
RGPSRTSITA LWEDIEIPAN SIVEARDLWE HQTLKQKFVG NLTATVDSHA
410
CKLYVLKPVA
Length:410
Mass (Da):45,709
Last modified:March 1, 2001 - v1
Checksum:iF68C52E35FC11F0A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78D → N in AAM13199 (PubMed:14593172).Curated1
Sequence conflicti78D → N in AAP37856 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL392174 Genomic DNA. Translation: CAC08338.1.
CP002688 Genomic DNA. Translation: AED91292.1.
AY093200 mRNA. Translation: AAM13199.1.
BT008497 mRNA. Translation: AAP37856.1.
RefSeqiNP_196455.1. NM_120922.5.
UniGeneiAt.10139.

Genome annotation databases

EnsemblPlantsiAT5G08380.1; AT5G08380.1; AT5G08380.
GeneIDi830736.
GrameneiAT5G08380.1; AT5G08380.1; AT5G08380.
KEGGiath:AT5G08380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL392174 Genomic DNA. Translation: CAC08338.1.
CP002688 Genomic DNA. Translation: AED91292.1.
AY093200 mRNA. Translation: AAM13199.1.
BT008497 mRNA. Translation: AAP37856.1.
RefSeqiNP_196455.1. NM_120922.5.
UniGeneiAt.10139.

3D structure databases

ProteinModelPortaliQ9FT97.
SMRiQ9FT97.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G08380.1.

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.

Proteomic databases

PaxDbiQ9FT97.
PRIDEiQ9FT97.
ProMEXiQ9FT97.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G08380.1; AT5G08380.1; AT5G08380.
GeneIDi830736.
GrameneiAT5G08380.1; AT5G08380.1; AT5G08380.
KEGGiath:AT5G08380.

Organism-specific databases

TAIRiAT5G08380.

Phylogenomic databases

eggNOGiKOG2366. Eukaryota.
ENOG410XPF1. LUCA.
HOGENOMiHOG000161224.
KOiK07407.
OMAiVAPHSCR.
OrthoDBiEOG09360A7S.
PhylomeDBiQ9FT97.

Enzyme and pathway databases

BioCyciARA:AT5G08380-MONOMER.
ReactomeiR-ATH-1660662. Glycosphingolipid metabolism.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ9FT97.

Gene expression databases

GenevisibleiQ9FT97. AT.

Family and domain databases

CDDicd14792. GH27. 1 hit.
Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_27/36_CS.
IPR013780. Glyco_hydro_b.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF16499. Melibiase_2. 1 hit.
[Graphical view]
PRINTSiPR00740. GLHYDRLASE27.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGAL1_ARATH
AccessioniPrimary (citable) accession number: Q9FT97
Secondary accession number(s): Q8RWB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 4, 2015
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.