Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

Gene

GNT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes an essential step in the conversion of oligo-mannose and hybrid to complex N-glycans.1 Publication

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 6-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • oligosaccharide biosynthetic process Source: UniProtKB
  • protein glycosylation Source: UniProtKB-UniPathway

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-ATH-975578. Reactions specific to the complex N-glycan synthesis pathway.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT16. Glycosyltransferase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferaseCurated (EC:2.4.1.1431 Publication)
Alternative name(s):
Beta-1,2-N-acetylglucosaminyltransferase II1 Publication
GlcNAc-T II1 Publication
Short name:
GNT-II1 Publication
Mannoside acetylglucosaminyltransferase 2Curated
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II1 Publication
Gene namesi
Name:GNT21 Publication
Ordered Locus Names:At2g05320Imported
ORF Names:F5G3.22Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G05320.
TAIRilocus:2051279. AT2G05320.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicCuratedAdd BLAST12
Transmembranei13 – 35Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST23
Topological domaini36 – 430LumenalCuratedAdd BLAST395

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004398321 – 430Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferaseAdd BLAST430

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi41N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi61N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi295N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G05320.1.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2791. Eukaryota.
ENOG410XPYF. LUCA.
HOGENOMiHOG000239578.
KOiK00736.
OMAiRDHELCK.
OrthoDBiEOG09360FIH.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiView protein in InterPro
IPR007754. GlcNAc_II.
IPR029044. Nucleotide-diphossugar_trans.
PANTHERiPTHR12871. PTHR12871. 1 hit.
PfamiView protein in Pfam
PF05060. MGAT2. 1 hit.
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9FT88-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANLWKKQRL RDTGLCRLGI LFAVTLSIVL MLVSVPRTAL NGSSIDDDLD
60 70 80 90 100
GLDKDLEAKL NASLLSVARG NRMSLRLHRR NHFSPRNTDL FPDLAKDRVV
110 120 130 140 150
IVLYVHNRAQ YFRVTVESLS KVKGISETLL IVSHDGYFEE MNRIVESIKF
160 170 180 190 200
CQVKQIFSPY SPHIYRTSFP GVTLNDCKNK GDEAKGHCEG NPDQYGNHRS
210 220 230 240 250
PKIVSLKHHW WWMMNTVWDG LEETKGHEGH ILFIEEDHFL FPNAYRNIQT
260 270 280 290 300
LTRLKPAKCP DCFAANLAPS DVKSRGEGLE SLVAERMGNV GYSFNRSVWE
310 320 330 340 350
NIHQKAREFC FFDDYNWDIT MWATVFPSFG SPVYTLRGPR TSAVHFGKCG
360 370 380 390 400
LHQGRGDEGD CIDNGVVNIE VKETDKVVNI KEGWGVRVYK HQAGYKAGFE
410 420 430
GWGGWGDDRD RHLCLDFATM YRYSSSSASP
Length:430
Mass (Da):48,947
Last modified:March 1, 2001 - v1
Checksum:i4CFF491AD490199D
GO

Sequence cautioni

The sequence AAD29068 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ249274 mRNA. Translation: CAC08806.1.
KJ138918 mRNA. Translation: AHL38858.1.
AC007018 Genomic DNA. Translation: AAD29068.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC05916.1.
PIRiB84467.
RefSeqiNP_178601.2. NM_126556.3.
UniGeneiAt.10148.

Genome annotation databases

EnsemblPlantsiAT2G05320.1; AT2G05320.1; AT2G05320.
GeneIDi815080.
GrameneiAT2G05320.1; AT2G05320.1; AT2G05320.
KEGGiath:AT2G05320.

Similar proteinsi

Entry informationi

Entry nameiMGAT2_ARATH
AccessioniPrimary (citable) accession number: Q9FT88
Secondary accession number(s): Q9SJ30
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2017
Last sequence update: March 1, 2001
Last modified: October 25, 2017
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families