Q9FT37 (TASY_TAXWC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Taxadiene synthase
EC=4.2.3.17

Alternative name(s):
Taxa-4(5),11(12)-diene synthase

Gene names

Name:

TDC1

Organism

Taxus wallichiana var. chinensis (Chinese yew) (Taxus chinensis)

Taxonomic identifier

29808 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Coniferophyta › Coniferopsida › Coniferales › Taxaceae › Taxus › Taxus wallichiana

Protein attributes

Sequence length	862 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the cyclization of the ubiquitous isoprenoid intermediate geranylgeranyl diphosphate to taxa-4,11-diene, the parent olefin with a taxane skeleton.
Catalytic activity	Geranylgeranyl diphosphate = taxa-4,11-diene + diphosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Pathway	Alkaloid biosynthesis; taxol biosynthesis; taxa-4(20),11-dien-5alpha-ol from geranylgeranyl diphosphate: step 1/2.
Domain	The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.
Sequence similarities	Belongs to the terpene synthase family.

Ontologies

Keywords
Biological process	Taxol biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Gene Ontology (GO)
Biological_process	paclitaxel biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: InterPro taxadiene synthase activity Inferred from electronic annotation. Source: EC terpene synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 862	862	Taxadiene synthase		PRO_0000186451
Regions
	Motif	613 – 617	5	DDXXD motif
Sites
	Metal binding	613	1	Magnesium 1 By similarity
	Metal binding	613	1	Magnesium 2 By similarity
	Metal binding	617	1	Magnesium 1 By similarity
	Metal binding	617	1	Magnesium 2 By similarity
	Metal binding	757	1	Magnesium 3 By similarity
	Metal binding	761	1	Magnesium 3 By similarity
	Metal binding	765	1	Magnesium 3 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9FT37 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 3A597BAF722BF679	FASTA	862	98,070
	10 20 30 40 50 60 MAQLSFNAAL KMNALGNKAI HDPTNCRAKS EGQMMWVCSK SGRTRVKMSR GSGGPGPVVM 70 80 90 100 110 120 MSSSTGTSKV VSETSSTIVD DIPRLSANYH GDLWHHNVIQ TLETPFRESS TYQERADELV 130 140 150 160 170 180 VKIKDMFNAL GDGDISPSAY DTAWVARVAT ISSDGSEKPR FPQALNWVFN NQLQDGSWGI 190 200 210 220 230 240 ESHFSLCDRL LNTTNSVIAL SVWKTGHSQV EQGTEFIAEN LRLLNEEDEL SPDFEIIFPA 250 260 270 280 290 300 LLQKAKALGI NLPYDLPFIK YLSTTREARL TDVSAAADNI PANMLNALEG LEEVMDWKKI 310 320 330 340 350 360 MRFQSKDGSF LSSPASTACV LMNTGDEKCF TFLNNLLVKF GGCVPCMYSI DLLERLSLVD 370 380 390 400 410 420 NIEHLGIGRH FKQEIKVALD YVYRHWSERG IGWGRDSLVP DLNTTALGLR TLRTHGYDVS 430 440 450 460 470 480 SDVLNNFKDE NGRFFSSAGQ THVELRSVVI LFRASDLAFP DEGAMDDARK FAEPYLRDAL 490 500 510 520 530 540 ATKISTNTKL FKEIEYVVEY PWHMSIPRSE ARSYIDSYDD DYVWERKTLY RMPSLSNSKC 550 560 570 580 590 600 LELAKLDFNI VQSLHQEELK LLTRWWKESG MADINFTRHR VAEVYFSSAT FEPEYSATRI 610 620 630 640 650 660 AFTKIGCLQV LFDDMADIFA TLDELKSFTE GVKRWDTSLL HEIPECMQTC FKVWFKLIEE 670 680 690 700 710 720 VNNDVVKVQG RDMLAHIRKP WELYFNCYVQ EREWLDAGYI PTFEEYLKTY AISVGLGPCT 730 740 750 760 770 780 LQPILLMGEL VKDDVVEKVH YPSNMFELVS LSWRLTNDTK TYQAEKARGQ QASGIACYMK 790 800 810 820 830 840 DNLGATEEDA IKHICRVVDR ALKEASFEYF KPSNDIPMGC KSFIFNLRLC VQIFYKFIDG 850 860 YGIANEEIKD YIRKVYIDPI QV

References

[1]	"Cloning, expression, and characterization of taxadiene synthase, a diteroene cyclase from Taxus chinensis." Wang W., Shi Q., Ouyang T., Zhu P., Cheng K. Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Callus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY007207 mRNA. Translation: AAG02257.1.
3D structure databases
ProteinModelPortal	Q9FT37.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14814.
BRENDA	4.2.3.17. 9720.
UniPathway	UPA00842; UER00806.
Family and domain databases
Gene3D	1.10.600.10. 1 hit. 1.50.30.10. 1 hit.
InterPro	IPR001906. Terpene_synth_N. IPR005630. Terpene_synthase_metal-bd. IPR008930. Terpenoid_cyclase/PrenylTrfase. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF01397. Terpene_synth. 1 hit. PF03936. Terpene_synth_C. 1 hit. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 2 hits. SSF48576. Terpenoid_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TASY_TAXWC

Accession

Primary (citable) accession number: Q9FT37

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 22, 2003
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program