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Protein

Malate dehydrogenase

Gene

MD1

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of the carbonyl group of oxalacetic acid. No activity with pulegone.1 Publication

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei106NADBy similarity1
Binding sitei113NADBy similarity1
Binding sitei132SubstratePROSITE-ProRule annotation1
Active sitei160Proton relayPROSITE-ProRule annotation1
Binding sitei163SubstratePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 18NADBy similarity7
Nucleotide bindingi130 – 132NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase (EC:1.1.1.37)
Short name:
NtRed-2
Gene namesi
Name:MD1
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004230251 – 332Malate dehydrogenaseAdd BLAST332

Proteomic databases

PRIDEiQ9FSF0.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9FSF0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Family and domain databases

CDDicd01336. MDH_cytoplasmic_cytosolic. 1 hit.
Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FSF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKDPVRVLV TGAAGQIGYA LVPMIARGVM LGADQPVILH MLDIPPAAEA
60 70 80 90 100
LNGVKMELVD AAFPLLKGVV ATTDAVEACT GVNVAVMVGG FPRKEGMERK
110 120 130 140 150
DVMSKNVSIY KSQASALEKH AAPNCKVLVV ANPANTNALI LKEYAPSIPE
160 170 180 190 200
KNISCLTRLD HNRALGQISE RLNVQVSDVK NVIIWGNHSS SQYPDVNHAT
210 220 230 240 250
VATPAGEKPV RELVADDAWL NGEFISTVQQ RGAAIIKARK LSSALSAASS
260 270 280 290 300
ACDHIRDWVL GTPEGTWVSM GVYSDGSYNV PAGLIYSFPV ACKNGEWSIV
310 320 330
QGLPIDEFSR KKLDATAEEL SEEKALAYSC LT
Length:332
Mass (Da):35,408
Last modified:March 1, 2001 - v1
Checksum:i726816BDB9049A9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ299256 mRNA. Translation: CAC12826.1.
RefSeqiNP_001312078.1. NM_001325149.1.
UniGeneiNta.6957.

Genome annotation databases

GeneIDi107772922.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ299256 mRNA. Translation: CAC12826.1.
RefSeqiNP_001312078.1. NM_001325149.1.
UniGeneiNta.6957.

3D structure databases

ProteinModelPortaliQ9FSF0.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ9FSF0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi107772922.

Family and domain databases

CDDicd01336. MDH_cytoplasmic_cytosolic. 1 hit.
Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDH_TOBAC
AccessioniPrimary (citable) accession number: Q9FSF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2013
Last sequence update: March 1, 2001
Last modified: October 5, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.