Reviewed, UniProtKB/Swiss-Prot Q9FRX4 (RIP1_CUCFI)

Names and origin

Protein names	Recommended name:     Putative ribosome-inactivating protein     EC=3.2.2.22 Alternative name(s):     rRNA N-glycosidase
Organism	Cucumis figarei
Taxonomic identifier	131071 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Cucumis

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Antiviral defense Plant defense
Domain	Signal
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW response to virus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 286	265	Putative ribosome-inactivating protein		PRO_0000030757
Sites
	Active site	185	1	By similarity
Amino acid modifications
	Glycosylation	103	1	N-linked (GlcNAc...) Potential
	Glycosylation	110	1	N-linked (GlcNAc...) Potential
	Glycosylation	252	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9FRX4-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 4EFD4966E604DA41	FASTA	286	31,772
	10 20 30 40 50 60 MNRFSVLMCL VILSIFHGVP TAEGDVTVKF SLLGSNHKSY SKFITSMRNA LPNAGDIYNI 70 80 90 100 110 120 PLLVPSISGS RRYILMQLSN YEGNTITMAV DVTNVYIMGY LVNGTSYFFN ETDAQLASKF 130 140 150 160 170 180 VFQGTKSITL PYSGNYQKLQ SVARKERDSI PLGFMALDSA ISTLYYYDSR SAPIAFLVLI 190 200 210 220 230 240 QTTAEAARYK YIEKQIIDRI SVSKVPDLAA ISLENEWSLL SKQIQIAKSN NGQFQTPVKI 250 260 270 280 INDKGILTEV TNVSSLVVTK NIMLLLNKLN IASFEDHVIS TTMPQA

References

[1]	"Cloning and analysis of a cDNA coding a putative ribosome-inactivating protein from Cucumis figarei." Yamada T., Ohki S.T., Osaki T. Plant Biotechnol. 17:337-340(2000) Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB045560 mRNA. Translation: BAB19677.1.
3D structure databases
HSSP	HSSP built from PDB template 1AHC based on UniProtKB P16094.
ModBase	Search...
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. IPR016138. Ribosome_inactivat_prot_sub1. IPR016139. Ribosome_inactivat_prot_sub2. IPR017989. Ribosome_inactivat_prot_subgr. [Graphical view]
Gene3D	G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit. G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PRINTS	PR00396. SHIGARICIN.
PROSITE	PS00275. SHIGA_RICIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIP1_CUCFI

Accession

Primary (citable) accession number: Q9FRX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)