UniProtKB - Q9FRL8 (DHAR2_ARATH)
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Protein
Glutathione S-transferase DHAR2
Gene
DHAR2
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Displays a dual function. As a soluble protein, exhibits glutathione-dependent thiol transferase and dehydroascorbate (DHA) reductase activities (PubMed:12077129). Exhibits glutathione-dependent thiol transferase and dehydroascorbate (DHA) reductase activities. Key component of the ascorbate recycling system. Involved in the redox homeostasis, especially in scavenging of ROS under oxidative stresses. Plays a role in ozone tolerance.2 Publications
Catalytic activityi
RX + glutathione = HX + R-S-glutathione.1 Publication
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 8 | AscorbateBy similarity | 1 | |
Binding sitei | 8 | GlutathioneBy similarity | 1 | |
Binding sitei | 19 | AscorbateBy similarity | 1 | |
Binding sitei | 19 | GlutathioneBy similarity | 1 | |
Active sitei | 20 | NucleophileBy similarity | 1 | |
Binding sitei | 47 | GlutathioneCombined sources1 Publication | 1 | |
Binding sitei | 60 | Glutathione; via amide nitrogen and carbonyl oxygenCombined sources1 Publication | 1 | |
Binding sitei | 73 | GlutathioneCombined sources1 Publication | 1 | |
Binding sitei | 160 | GlutathioneBy similarity | 1 | |
Binding sitei | 207 | GlutathioneBy similarity | 1 | |
Binding sitei | 210 | AscorbateBy similarity | 1 |
GO - Molecular functioni
- glutathione binding Source: TAIR
- glutathione dehydrogenase (ascorbate) activity Source: TAIR
- glutathione transferase activity Source: GO_Central
GO - Biological processi
- glutathione metabolic process Source: GO_Central
- positive regulation of salicylic acid mediated signaling pathway Source: TAIR
- protein glutathionylation Source: TAIR
Keywordsi
Molecular function | Oxidoreductase, Transferase |
Biological process | Detoxification, Stress response |
Enzyme and pathway databases
BioCyci | ARA:AT1G75270-MONOMER. |
Names & Taxonomyi
Protein namesi | Recommended name: Glutathione S-transferase DHAR2 (EC:2.5.1.181 Publication)Alternative name(s): Chloride intracellular channel homolog 2 Short name: CLIC homolog 2 Glutathione-dependent dehydroascorbate reductase 21 Publication (EC:1.8.5.11 Publication) Short name: AtDHAR21 Publication Short name: CytDHAR Short name: GSH-dependent dehydroascorbate reductase 2 |
Gene namesi | Name:DHAR2 Synonyms:DHAR Ordered Locus Names:At1g75270 ORF Names:F22H5.1 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G75270. |
TAIRi | locus:2025162. AT1G75270. |
Pathology & Biotechi
Disruption phenotypei
Mutant develops distinct foliar lesions under ozone exposure.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000395482 | 1 – 213 | Glutathione S-transferase DHAR2Add BLAST | 213 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 6 | S-glutathionyl cysteine1 Publication | 1 | |
Modified residuei | 20 | S-glutathionyl cysteine1 Publication | 1 |
Post-translational modificationi
Spontaneous S-glutathionylation in the presence of oxidized glutathione (GSSG).1 Publication
Keywords - PTMi
GlutathionylationProteomic databases
PaxDbi | Q9FRL8. |
PRIDEi | Q9FRL8. |
PTM databases
iPTMneti | Q9FRL8. |
SwissPalmi | Q9FRL8. |
Expressioni
Inductioni
By ozone. By salicylic acid (SA) (at protein level).2 Publications
Gene expression databases
ExpressionAtlasi | Q9FRL8. baseline and differential. |
Genevisiblei | Q9FRL8. AT. |
Interactioni
Subunit structurei
Monomer.By similarity
Protein-protein interaction databases
IntActi | Q9FRL8. 1 interactor. |
STRINGi | 3702.AT1G75270.1. |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Beta strandi | 7 – 12 | Combined sources | 6 | |
Helixi | 14 – 17 | Combined sources | 4 | |
Helixi | 21 – 32 | Combined sources | 12 | |
Beta strandi | 37 – 42 | Combined sources | 6 | |
Helixi | 49 – 54 | Combined sources | 6 | |
Beta strandi | 62 – 65 | Combined sources | 4 | |
Beta strandi | 68 – 71 | Combined sources | 4 | |
Helixi | 73 – 83 | Combined sources | 11 | |
Helixi | 93 – 95 | Combined sources | 3 | |
Turni | 96 – 101 | Combined sources | 6 | |
Helixi | 102 – 111 | Combined sources | 10 | |
Helixi | 119 – 136 | Combined sources | 18 | |
Beta strandi | 139 – 141 | Combined sources | 3 | |
Beta strandi | 144 – 146 | Combined sources | 3 | |
Helixi | 149 – 169 | Combined sources | 21 | |
Helixi | 178 – 188 | Combined sources | 11 | |
Helixi | 191 – 196 | Combined sources | 6 | |
Helixi | 200 – 207 | Combined sources | 8 | |
Helixi | 208 – 210 | Combined sources | 3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5LOL | X-ray | 2.30 | A | 1-213 | [»] | |
ProteinModelPortali | Q9FRL8. | |||||
SMRi | Q9FRL8. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 10 – 83 | GST N-terminalAdd BLAST | 74 | |
Domaini | 84 – 213 | GST C-terminalAdd BLAST | 130 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 20 – 25 | Glutathione-bindingSequence analysis | 6 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG410INGW. Eukaryota. ENOG411205E. LUCA. |
HOGENOMi | HOG000272670. |
InParanoidi | Q9FRL8. |
KOi | K21888. |
OMAi | SHTCPAD. |
OrthoDBi | EOG09360IQR. |
PhylomeDBi | Q9FRL8. |
Family and domain databases
InterProi | View protein in InterPro IPR010987. Glutathione-S-Trfase_C-like. IPR036282. Glutathione-S-Trfase_C_sf. IPR004045. Glutathione_S-Trfase_N. IPR036249. Thioredoxin-like_sf. |
Pfami | View protein in Pfam PF13417. GST_N_3. 1 hit. |
SUPFAMi | SSF47616. SSF47616. 1 hit. SSF52833. SSF52833. 1 hit. |
PROSITEi | View protein in PROSITE PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q9FRL8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALDICVKVA VGAPDVLGDC PFSQRVLLTL EEKKLPYKTH LINVSDKPQW
60 70 80 90 100
FLDISPEGKV PVVKLDGKWV ADSDVIVGLL EEKYPEPSLK TPPEFASVGS
110 120 130 140 150
KIFGAFVTFL KSKDANDGSE KALVDELEAL ENHLKTHSGP FVAGEKITAV
160 170 180 190 200
DLSLAPKLYH LEVALGHYKN WSVPESLTSV RNYAKALFSR ESFENTKAKK
210
EIVVAGWESK VNA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 195 | N → K in AAM65005 (Ref. 5) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY074785 mRNA. Translation: AAL71855.1. AC025814 Genomic DNA. Translation: AAG12679.1. CP002684 Genomic DNA. Translation: AEE35696.1. AY140019 mRNA. Translation: AAM98161.1. BT006257 mRNA. Translation: AAP13365.1. AY087460 mRNA. Translation: AAM65005.1. |
PIRi | B96783. |
RefSeqi | NP_177662.1. NM_106182.4. |
UniGenei | At.27979. |
Genome annotation databases
EnsemblPlantsi | AT1G75270.1; AT1G75270.1; AT1G75270. |
GeneIDi | 843864. |
Gramenei | AT1G75270.1; AT1G75270.1; AT1G75270. |
KEGGi | ath:AT1G75270. |
Similar proteinsi
Entry informationi
Entry namei | DHAR2_ARATH | |
Accessioni | Q9FRL8Primary (citable) accession number: Q9FRL8 Secondary accession number(s): Q8LB28 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 13, 2010 |
Last sequence update: | March 1, 2001 | |
Last modified: | March 28, 2018 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |