Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutathione S-transferase DHAR2

Gene

DHAR2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Displays a dual function. As a soluble protein, exhibits glutathione-dependent thiol transferase and dehydroascorbate (DHA) reductase activities (PubMed:12077129). Exhibits glutathione-dependent thiol transferase and dehydroascorbate (DHA) reductase activities. Key component of the ascorbate recycling system. Involved in the redox homeostasis, especially in scavenging of ROS under oxidative stresses. Plays a role in ozone tolerance.2 Publications

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei8AscorbateBy similarity1
Binding sitei8GlutathioneBy similarity1
Binding sitei19AscorbateBy similarity1
Binding sitei19GlutathioneBy similarity1
Active sitei20NucleophileBy similarity1
Binding sitei47GlutathioneCombined sources1 Publication1
Binding sitei60Glutathione; via amide nitrogen and carbonyl oxygenCombined sources1 Publication1
Binding sitei73GlutathioneCombined sources1 Publication1
Binding sitei160GlutathioneBy similarity1
Binding sitei207GlutathioneBy similarity1
Binding sitei210AscorbateBy similarity1

GO - Molecular functioni

  • glutathione binding Source: TAIR
  • glutathione dehydrogenase (ascorbate) activity Source: TAIR
  • glutathione transferase activity Source: GO_Central

GO - Biological processi

  • glutathione metabolic process Source: GO_Central
  • protein glutathionylation Source: TAIR

Keywordsi

Molecular functionOxidoreductase, Transferase
Biological processDetoxification, Stress response

Enzyme and pathway databases

BioCyciARA:AT1G75270-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase DHAR2 (EC:2.5.1.181 Publication)
Alternative name(s):
Chloride intracellular channel homolog 2
Short name:
CLIC homolog 2
Glutathione-dependent dehydroascorbate reductase 21 Publication (EC:1.8.5.11 Publication)
Short name:
AtDHAR21 Publication
Short name:
CytDHAR
Short name:
GSH-dependent dehydroascorbate reductase 2
Gene namesi
Name:DHAR2
Synonyms:DHAR
Ordered Locus Names:At1g75270
ORF Names:F22H5.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G75270.
TAIRilocus:2025162. AT1G75270.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mutant develops distinct foliar lesions under ozone exposure.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003954821 – 213Glutathione S-transferase DHAR2Add BLAST213

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6S-glutathionyl cysteine1 Publication1
Modified residuei20S-glutathionyl cysteine1 Publication1

Post-translational modificationi

Spontaneous S-glutathionylation in the presence of oxidized glutathione (GSSG).1 Publication

Keywords - PTMi

Glutathionylation

Proteomic databases

PaxDbiQ9FRL8.
PRIDEiQ9FRL8.

PTM databases

iPTMnetiQ9FRL8.
SwissPalmiQ9FRL8.

Expressioni

Inductioni

By ozone. By salicylic acid (SA) (at protein level).2 Publications

Gene expression databases

GenevisibleiQ9FRL8. AT.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiQ9FRL8. 1 interactor.
STRINGi3702.AT1G75270.1.

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 12Combined sources6
Helixi14 – 17Combined sources4
Helixi21 – 32Combined sources12
Beta strandi37 – 42Combined sources6
Helixi49 – 54Combined sources6
Beta strandi62 – 65Combined sources4
Beta strandi68 – 71Combined sources4
Helixi73 – 83Combined sources11
Helixi93 – 95Combined sources3
Turni96 – 101Combined sources6
Helixi102 – 111Combined sources10
Helixi119 – 136Combined sources18
Beta strandi139 – 141Combined sources3
Beta strandi144 – 146Combined sources3
Helixi149 – 169Combined sources21
Helixi178 – 188Combined sources11
Helixi191 – 196Combined sources6
Helixi200 – 207Combined sources8
Helixi208 – 210Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LOLX-ray2.30A1-213[»]
ProteinModelPortaliQ9FRL8.
SMRiQ9FRL8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 83GST N-terminalAdd BLAST74
Domaini84 – 213GST C-terminalAdd BLAST130

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi20 – 25Glutathione-bindingSequence analysis6

Sequence similaritiesi

Belongs to the GST superfamily. DHAR family.Curated

Phylogenomic databases

eggNOGiENOG410INGW. Eukaryota.
ENOG411205E. LUCA.
HOGENOMiHOG000272670.
InParanoidiQ9FRL8.
KOiK21888.
OMAiQDDALYQ.
OrthoDBiEOG09360IQR.
PhylomeDBiQ9FRL8.

Family and domain databases

InterProiView protein in InterPro
IPR010987. Glutathione-S-Trfase_C-like.
IPR036282. Glutathione-S-Trfase_C_sf.
IPR004045. Glutathione_S-Trfase_N.
IPR036249. Thioredoxin-like_sf.
PfamiView protein in Pfam
PF13417. GST_N_3. 1 hit.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9FRL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALDICVKVA VGAPDVLGDC PFSQRVLLTL EEKKLPYKTH LINVSDKPQW
60 70 80 90 100
FLDISPEGKV PVVKLDGKWV ADSDVIVGLL EEKYPEPSLK TPPEFASVGS
110 120 130 140 150
KIFGAFVTFL KSKDANDGSE KALVDELEAL ENHLKTHSGP FVAGEKITAV
160 170 180 190 200
DLSLAPKLYH LEVALGHYKN WSVPESLTSV RNYAKALFSR ESFENTKAKK
210
EIVVAGWESK VNA
Length:213
Mass (Da):23,407
Last modified:March 1, 2001 - v1
Checksum:i23543B0F81F96041
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti195N → K in AAM65005 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY074785 mRNA. Translation: AAL71855.1.
AC025814 Genomic DNA. Translation: AAG12679.1.
CP002684 Genomic DNA. Translation: AEE35696.1.
AY140019 mRNA. Translation: AAM98161.1.
BT006257 mRNA. Translation: AAP13365.1.
AY087460 mRNA. Translation: AAM65005.1.
PIRiB96783.
RefSeqiNP_177662.1. NM_106182.4.
UniGeneiAt.27979.

Genome annotation databases

EnsemblPlantsiAT1G75270.1; AT1G75270.1; AT1G75270.
GeneIDi843864.
GrameneiAT1G75270.1; AT1G75270.1; AT1G75270.
KEGGiath:AT1G75270.

Similar proteinsi

Entry informationi

Entry nameiDHAR2_ARATH
AccessioniPrimary (citable) accession number: Q9FRL8
Secondary accession number(s): Q8LB28
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: March 1, 2001
Last modified: October 25, 2017
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families