Q9FRH7 (TLP10_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tubby-like F-box protein 10
Short name=AtTLP10

Alternative name(s):
SKP1-interacting partner 26

Gene names

Name:	TULP10
Synonyms:	SKIP26
Ordered Locus Names:	At1g25280
ORF Names:	F4F7.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (ASK-cullin-F-box) protein ligase complex By similarity. Interacts with SKP1A/ASK1. Ref.7
Subcellular location	Nucleus By similarity.
Tissue specificity	Ubiquitous. Ref.1
Domain	The F-box is necessary for the interaction with ASK proteins By similarity.
Sequence similarities	Belongs to the TUB family. Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway response to fungus Inferred from mutant phenotype PubMed 22751378. Source: TAIR
Cellular_component	cytosol Inferred from direct assay PubMed 22751378. Source: TAIR nucleus Inferred from direct assay PubMed 22751378. Source: TAIR plasma membrane Inferred from direct assay PubMed 17317660PubMed 22751378. Source: TAIR plastid Inferred from direct assay PubMed 22751378. Source: TAIR
Molecular_function	sequence-specific DNA binding transcription factor activity Inferred from sequence or structural similarity PubMed 11118137. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9FRH7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	Tubby-like F-box protein 10		PRO_0000272238
Regions
	Domain	57 – 112	56	F-box
Natural variations
	Natural variant	114	1	L → I in strain: cv. Lisse. Ref.4
	Natural variant	244	1	Y → F in strain: cv. Bretagny. Ref.4
Experimental info
	Sequence conflict	404	1	K → E in BAH19883. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: C77BEA9ED18B9D21	FASTA	445	50,010
	10 20 30 40 50 60 MSFRGIVQDL RDGFGSLSRR SFDFRLSSLH KGKAQGSSFR EYSSSRDLLS PVIVQTSRWA 70 80 90 100 110 120 NLPPELLFDV IKRLEESESN WPARKHVVAC ASVCRSWRAM CQEIVLGPEI CGKLTFPVSL 130 140 150 160 170 180 KQPGPRDAMI QCFIKRDKSK LTFHLFLCLS PALLVENGKF LLSAKRTRRT TRTEYIISMD 190 200 210 220 230 240 ADNISRSSNS YLGKLRSNFL GTKFLVYDTQ PPPNTSSSAL ITDRTSRSRF HSRRVSPKVP 250 260 270 280 290 300 SGSYNIAQIT YELNVLGTRG PRRMHCIMNS IPISSLEPGG SVPNQPEKLV PAPYSLDDSF 310 320 330 340 350 360 RSNISFSKSS FDHRSLDFSS SRFSEMGISC DDNEEEASFR PLILKNKQPR WHEQLQCWCL 370 380 390 400 410 420 NFRGRVTVAS VKNFQLVAAR QPQPQGTGAA AAPTSAPAHP EQDKVILQFG KVGKDMFTMD 430 440 YRYPLSAFQA FAICLSSFDT KLACE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular analyses of the Arabidopsis TUBBY-like protein gene family." Lai C.-P., Lee C.-L., Chen P.-H., Wu S.-H., Yang C.-C., Shaw J.-F. Plant Physiol. 134:1586-1597(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Selection on rapidly evolving proteins in the Arabidopsis genome." Barrier M., Bustamante C.D., Yu J., Purugganan M.D. Genetics 163:723-733(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 18-432, VARIANTS ILE-114 AND PHE-244. Strain: cv. Bla-1, cv. Bretagny, cv. Bs-1, cv. Bu-0, cv. Chi-1, cv. Co-1, cv. Hau-0, cv. Jl-1, cv. Kas-1, cv. Kent and cv. Lisse.
[5]	"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs." Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K. DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 129-445. Strain: cv. Columbia.
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 179-445. Strain: cv. Columbia.
[7]	"Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis." Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L. Plant J. 34:753-767(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SKP1A/ASK1.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF487271 mRNA. Translation: AAQ06244.1. AC079374 Genomic DNA. Translation: AAG28805.1. CP002684 Genomic DNA. Translation: AEE30599.1. AY140459 Genomic DNA. Translation: AAN46223.1. AY140460 Genomic DNA. Translation: AAN46224.1. AY140461 Genomic DNA. Translation: AAN46225.1. AY140462 Genomic DNA. Translation: AAN46226.1. AY140463 Genomic DNA. Translation: AAN46227.1. AY140464 Genomic DNA. Translation: AAN46228.1. AY140465 Genomic DNA. Translation: AAN46229.1. AY140466 Genomic DNA. Translation: AAN46230.1. AY140467 Genomic DNA. Translation: AAN46231.1. AY140468 Genomic DNA. Translation: AAN46232.1. AY140469 Genomic DNA. Translation: AAN46233.1. AK317199 mRNA. Translation: BAH19883.1. BT001997 mRNA. Translation: AAN72008.1. BT006290 mRNA. Translation: AAP13398.1.
IPI	IPI00539910.
PIR	E86382.
RefSeq	NP_001117353.1. NM_001123881.1. NP_173899.1. NM_102338.2. NP_973909.1. NM_202180.1.
UniGene	At.23717.
3D structure databases
HSSP	HSSP built from PDB template 2FIM based on UniProtKB O00294.
ProteinModelPortal	Q9FRH7.
SMR	Q9FRH7. Positions 122-445.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9FRH7. 2 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G25280.1; AT1G25280.1; AT1G25280.
GeneID	839112.
KEGG	ath:AT1G25280.
Organism-specific databases
TAIR	At1g25280.
Phylogenomic databases
eggNOG	NOG286778.
HOGENOM	HOG000238161.
InParanoid	Q9FRH7.
OMA	IQESRWA.
PhylomeDB	Q9FRH7.
ProtClustDB	CLSN2681282.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q9FRH7.
Genevestigator	Q9FRH7.
Family and domain databases
Gene3D	3.20.90.10. 2 hits.
InterPro	IPR001810. F-box_dom_cyclin-like. IPR000007. Tubby_C. IPR025659. Tubby_C-like. IPR018066. Tubby_C_CS. [Graphical view]
Pfam	PF00646. F-box. 1 hit. PF01167. Tub. 1 hit. [Graphical view]
PRINTS	PR01573. SUPERTUBBY.
SUPFAM	SSF54518. Tubby_C. 1 hit.
PROSITE	PS50181. FBOX. False negative. PS01200. TUB_1. 1 hit. PS01201. TUB_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TLP10_ARATH

Accession

Primary (citable) accession number: Q9FRH7
Secondary accession number(s): B9DGL6 , Q84JM8, Q84UG2, Q84UG3, Q8H0W5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program