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Protein

Delta(8)-fatty-acid desaturase

Gene

sld1

Organism
Borago officinalis (Bourrache) (Borage)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays a major role as delta8-fatty-acid desaturase which introduces a double bond at the 8-position in the long-chain base (LCB) of ceramides with or without a hydroxy group at the 4-position. The enzyme produces both the 8E and 8Z isomers (in a 4:1 ratio). This structural modification contributes to the quantitative partitioning of ceramides between the two major sphingolipid classes, glucosylceramides and glycosylinositolphosphoryl ceramides. Sphingolipids are important membrane components involved in environmental stress responses, such as resistance to chilling, and act as cell signaling molecules.2 Publications

Catalytic activityi

A (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O.1 Publication
A (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O.1 Publication

Cofactori

Fe cationBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi63Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Lipid metabolism, Sphingolipid metabolism, Transport
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.14.19.4. 895.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta(8)-fatty-acid desaturase (EC:1.14.19.291 Publication)
Alternative name(s):
Delta(8)-sphingolipid desaturase
Sphingolipid 8-(E/Z)-desaturase
Gene namesi
Name:sld1
OrganismiBorago officinalis (Bourrache) (Borage)
Taxonomic identifieri13363 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsBoraginalesBoraginaceaeBoraginoideaeBoragineaeBoragininaeBorago

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei112 – 132HelicalSequence analysisAdd BLAST21
Transmembranei136 – 156HelicalSequence analysisAdd BLAST21
Transmembranei174 – 195HelicalSequence analysisAdd BLAST22
Transmembranei253 – 273HelicalSequence analysisAdd BLAST21
Transmembranei282 – 302HelicalSequence analysisAdd BLAST21
Transmembranei309 – 329HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004188921 – 446Delta(8)-fatty-acid desaturaseAdd BLAST446

Expressioni

Tissue specificityi

Expressed only in young leaves.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9FR82.
SMRiQ9FR82.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 89Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST85

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi158 – 162Histidine box-1Curated5
Motifi195 – 199Histidine box-2Curated5
Motifi372 – 376Histidine box-3Curated5

Domaini

The histidine box domains may contain the active site and/or be involved in metal ion binding.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiView protein in InterPro
IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR036400. Cyt_B5-like_heme/steroid_sf.
IPR005804. FA_desaturase_dom.
IPR012171. Fatty_acid_desaturase.
PfamiView protein in Pfam
PF00173. Cyt-b5. 1 hit.
PF00487. FA_desaturase. 1 hit.
PIRSFiPIRSF015921. FA_sphinglp_des. 1 hit.
SMARTiView protein in SMART
SM01117. Cyt-b5. 1 hit.
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiView protein in PROSITE
PS50255. CYTOCHROME_B5_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9FR82-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGTKKYISV GELEKHNQLG DVWISIQGKV YNVTDWIKKH PGGDVPIMNL
60 70 80 90 100
AGQDATDAFI AYHPGTAWKN LENLFTGYHL EDYLVSEISK DYRKLASEFS
110 120 130 140 150
KAGLFEKKGH TVIYCLSFIA LLLCGCVYGV LCSNSLWVHM LSGAMLGMCF
160 170 180 190 200
IQAAYLGHDS GHYTMMSSKG YNKFAQVLNG NCLTGISIAW WKWTHNAHHI
210 220 230 240 250
ACNSLDYDPD LQHLPVFAVP SSFFKSLTSR FYGRELTFDG LSRFLVSYQH
260 270 280 290 300
FTIYLVMIFG RINLYVQTFL LLFSTRKVPD RALNIIGILV YWTWFPYLVS
310 320 330 340 350
CLPNWNERVL FVLTCFSVTA LQHIQFTLNH FAADVYVGPP TGTNWFEKQA
360 370 380 390 400
AGTIDISCSS WMDWFFGGLQ FQLEHHLFPR MPRCQLRNIS PIVQDYCKKH
410 420 430 440
NLPYRSLSFF DANVATIKTL RTAALQARDL TVVPQNLLWE AFNTHG
Length:446
Mass (Da):50,927
Last modified:March 1, 2001 - v1
Checksum:iEBD579F035A3AF0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF133728 mRNA. Translation: AAG43277.1.

Similar proteinsi

Entry informationi

Entry nameiSLD1_BOROF
AccessioniPrimary (citable) accession number: Q9FR82
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: March 1, 2001
Last modified: October 25, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families