Q9FQY8 (COMT1_CAPAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Caffeic acid 3-O-methyltransferase
Short name=CAOMT
Short name=COMT
EC=2.1.1.68

Alternative name(s):
S-adenosysl-L-methionine:caffeic acid 3-O-methyltransferase

Gene names

Name:

COMT

Organism

Capsicum annuum (Bell pepper)

Taxonomic identifier

4072 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Capsiceae › Capsicum

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the conversion of caffeic acid to ferulic acid and of 5-hydroxyferulic acid to sinapic acid. The resulting products may subsequently be converted to the corresponding alcohols that are incorporated into lignins.
Catalytic activity	S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate.
Pathway	Aromatic compound metabolism; phenylpropanoid biosynthesis.
Subunit structure	Homodimer By similarity.
Tissue specificity	Fruit. Not expressed in leaf.
Developmental stage	Expression increases during fruit development but decreases during ripening.
Sequence similarities	Belongs to the methyltransferase superfamily. Type 2 family. COMT subfamily.

Ontologies

Keywords
Biological process	Lignin biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	lignin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	caffeate O-methyltransferase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Caffeic acid 3-O-methyltransferase		PRO_0000063196
Regions
	Region	126 – 132	7	Substrate binding By similarity
	Region	158 – 176	19	Substrate binding By similarity
Sites
	Active site	265	1	Proton acceptor By similarity
	Binding site	204	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	227	1	S-adenosyl-L-methionine By similarity
	Binding site	247	1	S-adenosyl-L-methionine By similarity
	Binding site	248	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	261	1	S-adenosyl-L-methionine By similarity
Experimental info
	Sequence conflict	71	1	V → I in AAG43822. Ref.2
	Sequence conflict	246	1	R → G in AAG43822. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9FQY8 [UniParc]. Last modified April 30, 2003. Version 2. Checksum: 70599AED75CE1730	FASTA	359	39,433
	10 20 30 40 50 60 MDSTNQNLTQ TEDEAFLFAM QLASASVLPM VLKSALELDL LEIMAKAGPG AAISPSELAA 70 80 90 100 110 120 QLPTKNPEAP VMLDRMLRLL ATYSVLNCTL RTLPDGRVER LYSLAPVCKL LTKNADGVSV 130 140 150 160 170 180 APLLLMNQDK VLMESWYHLT DAVLDGGVPF NKAYGMTAFE YHGTDPRFNK VFNRGMSDHS 190 200 210 220 230 240 TMTMKKILED YKGFEGLNSI VDVGGGTGAT VNMIVSKYPS IKGINFDLSH VIEDAPAYPG 250 260 270 280 290 300 VEHVGRDMFV SVPKADAIFM KWICHDWSDE HCLKFLKNCY EALPANGKVL VAECILPETP 310 320 330 340 350 DTSAATKNAV HVDIVMLAHN PGGKERTEKE FEALAKGAGF TGFRRACCAY QTWVMEFHK

References

[1]	"Isolation and characterization of o-diphenol-O-methyltransferase cDNA clone in hot pepper (Capsicum annuum L.)." Lee B.-H., Choi D., Lee K.-W. J. Plant Biol. 41:9-14(1998) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pericarp.
[2]	"Isolation and characterization of caffeic acid O-methyltrasferase cDNA from Capsicum annuum." Kim K.-W., Lee S.-W. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Chungyang. Tissue: Root.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U83789 mRNA. Translation: AAC17455.1. AF212316 mRNA. Translation: AAG43822.1.
PIR	T12259.
3D structure databases
ProteinModelPortal	Q9FQY8.
SMR	Q9FQY8. Positions 11-359.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
InterPro	IPR016461. O-MeTrfase_COMT_euk. IPR001077. O_MeTrfase_2. IPR012967. Plant_MeTrfase_dimerisation. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Pfam	PF08100. Dimerisation. 1 hit. PF00891. Methyltransf_2. 1 hit. [Graphical view]
PIRSF	PIRSF005739. O-mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COMT1_CAPAN

Accession

Primary (citable) accession number: Q9FQY8
Secondary accession number(s): P93088

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	April 30, 2003
Last modified:	April 18, 2012
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program