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Protein

ATP-dependent DNA helicase 2 subunit KU70

Gene

KU70

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Single-stranded DNA-dependent ATP-dependent helicase. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair. When associated with KU80, binds to double-stranded telomeric and non-telomeric DNA sequences, but not to single-stranded DNA. Plays a role in maintaining telomere length. Acts as a negative regulator of telomerase. Required for maintenance of the telomeric C-rich strand.5 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

GO - Molecular functioni

GO - Biological processi

  • DNA recombination Source: UniProtKB-KW
  • DNA repair Source: TAIR
  • double-strand break repair via nonhomologous end joining Source: TAIR
  • response to heat Source: TAIR
  • telomere maintenance Source: TAIR

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA recombination, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-5693571 Nonhomologous End-Joining (NHEJ)
R-ATH-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase 2 subunit KU70 (EC:3.6.4.12)
Alternative name(s):
ATP-dependent DNA helicase 2 subunit 1
ATP-dependent DNA helicase II 70 kDa subunit
Gene namesi
Name:KU70
Ordered Locus Names:At1g16970
ORF Names:F6I1.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G16970
TAIRilocus:2035495 AT1G16970

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype when grown under normal conditions. Hypersensitivity to ionising radiation (IR) and to methylmethane sulfonate (MMS). Longer telomeres.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi117R → K: Loss of interactions with TRP1 and KU80; when associated with R-319. 1 Publication1
Mutagenesisi319G → R: Loss of interactions with TRP1 and KU80; when associated with K-117. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003941301 – 621ATP-dependent DNA helicase 2 subunit KU70Add BLAST621

Proteomic databases

PaxDbiQ9FQ08
PRIDEiQ9FQ08

PTM databases

iPTMnetiQ9FQ08

Expressioni

Tissue specificityi

Expressed ubiquitously.2 Publications

Inductioni

Up-regulated in response to induction of double-strand breaks. Down-regulated by heat shock.2 Publications

Gene expression databases

ExpressionAtlasiQ9FQ08 baseline and differential
GenevisibleiQ9FQ08 AT

Interactioni

Subunit structurei

Heterodimer with KU80. Interacts with TRP1. Interacts with WEX. Interacts with OFP1.6 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi23508, 4 interactors
IntActiQ9FQ08, 3 interactors
STRINGi3702.AT1G16970.1

Structurei

3D structure databases

ProteinModelPortaliQ9FQ08
SMRiQ9FQ08
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini273 – 471KuAdd BLAST199
Domaini585 – 619SAPPROSITE-ProRule annotationAdd BLAST35

Sequence similaritiesi

Belongs to the ku70 family.Curated

Phylogenomic databases

eggNOGiKOG2327 Eukaryota
ENOG410XNXU LUCA
HOGENOMiHOG000030793
InParanoidiQ9FQ08
KOiK10884
OMAiDVFTSCN
OrthoDBiEOG0936071J
PhylomeDBiQ9FQ08

Family and domain databases

Gene3Di1.10.720.30, 1 hit
2.40.290.10, 2 hits
3.40.50.410, 1 hit
4.10.970.10, 1 hit
InterProiView protein in InterPro
IPR006165 Ku70
IPR006164 Ku70/Ku80_beta-barrel_dom
IPR027388 Ku70_bridge/pillars_dom_sf
IPR005160 Ku_C
IPR005161 Ku_N
IPR003034 SAP_dom
IPR036361 SAP_dom_sf
IPR016194 SPOC-like_C_dom_sf
IPR036465 vWFA_dom_sf
PANTHERiPTHR12604:SF2 PTHR12604:SF2, 1 hit
PfamiView protein in Pfam
PF02735 Ku, 1 hit
PF03730 Ku_C, 1 hit
PF03731 Ku_N, 1 hit
PF02037 SAP, 1 hit
PIRSFiPIRSF003033 Ku70, 1 hit
SMARTiView protein in SMART
SM00559 Ku78, 1 hit
SM00513 SAP, 1 hit
SUPFAMiSSF100939 SSF100939, 1 hit
SSF53300 SSF53300, 1 hit
SSF68906 SSF68906, 1 hit
TIGRFAMsiTIGR00578 ku70, 1 hit
PROSITEiView protein in PROSITE
PS50800 SAP, 1 hit

Sequencei

Sequence statusi: Complete.

Q9FQ08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELDPDDVFR DEDEDPENDF FQEKEASKEF VVYLIDASPK MFCSTCPSEE
60 70 80 90 100
EDKQESHFHI AVSCIAQSLK AHIINRSNDE IAICFFNTRE KKNLQDLNGV
110 120 130 140 150
YVFNVPERDS IDRPTARLIK EFDLIEESFD KEIGSQTGIV SDSRENSLYS
160 170 180 190 200
ALWVAQALLR KGSLKTADKR MFLFTNEDDP FGSMRISVKE DMTRTTLQRA
210 220 230 240 250
KDAQDLGISI ELLPLSQPDK QFNITLFYKD LIGLNSDELT EFMPSVGQKL
260 270 280 290 300
EDMKDQLKKR VLAKRIAKRI TFVICDGLSI ELNGYALLRP AIPGSITWLD
310 320 330 340 350
STTNLPVKVE RSYICTDTGA IMQDPIQRIQ PYKNQNIMFT VEELSQVKRI
360 370 380 390 400
STGHLRLLGF KPLSCLKDYH NLKPSTFLYP SDKEVIGSTR AFIALHRSMI
410 420 430 440 450
QLERFAVAFY GGTTPPRLVA LVAQDEIESD GGQVEPPGIN MIYLPYANDI
460 470 480 490 500
RDIDELHSKP GVAAPRASDD QLKKASALMR RLELKDFSVC QFANPALQRH
510 520 530 540 550
YAILQAIALD ENELRETRDE TLPDEEGMNR PAVVKAIEQF KQSIYGDDPD
560 570 580 590 600
EESDSGAKEK SKKRKAGDAD DGKYDYIELA KTGKLKDLTV VELKTYLTAN
610 620
NLLVSGKKEV LINRILTHIG K
Length:621
Mass (Da):70,291
Last modified:March 1, 2001 - v1
Checksum:iAF1173E3DE189396
GO

Sequence cautioni

The sequence AAF99835 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF283759 mRNA Translation: AAG44852.1
AC051629 Genomic DNA Translation: AAF99835.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE29527.1
AK221642 mRNA Translation: BAD95294.1
PIRiD86305
RefSeqiNP_564012.1, NM_101558.4
UniGeneiAt.22247

Genome annotation databases

EnsemblPlantsiAT1G16970.1; AT1G16970.1; AT1G16970
GeneIDi838268
GrameneiAT1G16970.1; AT1G16970.1; AT1G16970
KEGGiath:AT1G16970

Similar proteinsi

Entry informationi

Entry nameiKU70_ARATH
AccessioniPrimary (citable) accession number: Q9FQ08
Secondary accession number(s): Q9FZ56
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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