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Protein

ATP-dependent DNA helicase 2 subunit KU70

Gene

KU70

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Single-stranded DNA-dependent ATP-dependent helicase. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair. When associated with KU80, binds to double-stranded telomeric and non-telomeric DNA sequences, but not to single-stranded DNA. Plays a role in maintaining telomere length. Acts as a negative regulator of telomerase. Required for maintenance of the telomeric C-rich strand.5 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

GO - Molecular functioni

GO - Biological processi

  • cellular response to gamma radiation Source: GO_Central
  • cellular response to X-ray Source: GO_Central
  • DNA duplex unwinding Source: GO_Central
  • DNA recombination Source: GO_Central
  • DNA repair Source: TAIR
  • double-strand break repair via nonhomologous end joining Source: TAIR
  • response to heat Source: TAIR
  • telomere maintenance Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-1986-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase 2 subunit KU70 (EC:3.6.4.12)
Alternative name(s):
ATP-dependent DNA helicase 2 subunit 1
ATP-dependent DNA helicase II 70 kDa subunit
Gene namesi
Name:KU70
Ordered Locus Names:At1g16970
ORF Names:F6I1.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G16970.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

  • Note: Predominantly in the nucleus.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype when grown under normal conditions. Hypersensitivity to ionising radiation (IR) and to methylmethane sulfonate (MMS). Longer telomeres.3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi117 – 1171R → K: Loss of interactions with TRP1 and KU80; when associated with R-319. 1 Publication
Mutagenesisi319 – 3191G → R: Loss of interactions with TRP1 and KU80; when associated with K-117. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 621621ATP-dependent DNA helicase 2 subunit KU70PRO_0000394130Add
BLAST

Proteomic databases

PaxDbiQ9FQ08.
PRIDEiQ9FQ08.

PTM databases

iPTMnetiQ9FQ08.

Expressioni

Tissue specificityi

Expressed ubiquitously.2 Publications

Inductioni

Up-regulated in response to induction of double-strand breaks. Down-regulated by heat shock.2 Publications

Gene expression databases

GenevisibleiQ9FQ08. AT.

Interactioni

Subunit structurei

Heterodimer with KU80. Interacts with TRP1. Interacts with WEX. Interacts with OFP1.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TRP1Q8L7L82EBI-476083,EBI-476071
WEXQ84LH32EBI-476083,EBI-926580

Protein-protein interaction databases

BioGridi23508. 3 interactions.
IntActiQ9FQ08. 3 interactions.
MINTiMINT-1212489.
STRINGi3702.AT1G16970.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FQ08.
SMRiQ9FQ08. Positions 28-618.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini273 – 471199KuAdd
BLAST
Domaini585 – 61935SAPPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ku70 family.Curated
Contains 1 Ku domain.Curated
Contains 1 SAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2327. Eukaryota.
ENOG410XNXU. LUCA.
HOGENOMiHOG000030793.
InParanoidiQ9FQ08.
KOiK10884.
OMAiRKAYKFG.
PhylomeDBiQ9FQ08.

Family and domain databases

Gene3Di1.10.1600.10. 1 hit.
1.10.720.30. 1 hit.
2.40.290.10. 1 hit.
3.40.50.410. 1 hit.
InterProiIPR006165. Ku70.
IPR006164. Ku70/Ku80_beta-barrel_dom.
IPR005160. Ku_C.
IPR005161. Ku_N.
IPR003034. SAP_dom.
IPR016194. SPOC-like_C_dom.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR12604:SF2. PTHR12604:SF2. 1 hit.
PfamiPF02735. Ku. 1 hit.
PF03730. Ku_C. 1 hit.
PF03731. Ku_N. 1 hit.
PF02037. SAP. 1 hit.
[Graphical view]
PIRSFiPIRSF003033. Ku70. 1 hit.
SMARTiSM00559. Ku78. 1 hit.
SM00513. SAP. 1 hit.
[Graphical view]
SUPFAMiSSF100939. SSF100939. 1 hit.
SSF53300. SSF53300. 1 hit.
TIGRFAMsiTIGR00578. ku70. 1 hit.
PROSITEiPS50800. SAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FQ08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELDPDDVFR DEDEDPENDF FQEKEASKEF VVYLIDASPK MFCSTCPSEE
60 70 80 90 100
EDKQESHFHI AVSCIAQSLK AHIINRSNDE IAICFFNTRE KKNLQDLNGV
110 120 130 140 150
YVFNVPERDS IDRPTARLIK EFDLIEESFD KEIGSQTGIV SDSRENSLYS
160 170 180 190 200
ALWVAQALLR KGSLKTADKR MFLFTNEDDP FGSMRISVKE DMTRTTLQRA
210 220 230 240 250
KDAQDLGISI ELLPLSQPDK QFNITLFYKD LIGLNSDELT EFMPSVGQKL
260 270 280 290 300
EDMKDQLKKR VLAKRIAKRI TFVICDGLSI ELNGYALLRP AIPGSITWLD
310 320 330 340 350
STTNLPVKVE RSYICTDTGA IMQDPIQRIQ PYKNQNIMFT VEELSQVKRI
360 370 380 390 400
STGHLRLLGF KPLSCLKDYH NLKPSTFLYP SDKEVIGSTR AFIALHRSMI
410 420 430 440 450
QLERFAVAFY GGTTPPRLVA LVAQDEIESD GGQVEPPGIN MIYLPYANDI
460 470 480 490 500
RDIDELHSKP GVAAPRASDD QLKKASALMR RLELKDFSVC QFANPALQRH
510 520 530 540 550
YAILQAIALD ENELRETRDE TLPDEEGMNR PAVVKAIEQF KQSIYGDDPD
560 570 580 590 600
EESDSGAKEK SKKRKAGDAD DGKYDYIELA KTGKLKDLTV VELKTYLTAN
610 620
NLLVSGKKEV LINRILTHIG K
Length:621
Mass (Da):70,291
Last modified:March 1, 2001 - v1
Checksum:iAF1173E3DE189396
GO

Sequence cautioni

The sequence AAF99835.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF283759 mRNA. Translation: AAG44852.1.
AC051629 Genomic DNA. Translation: AAF99835.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29527.1.
AK221642 mRNA. Translation: BAD95294.1.
PIRiD86305.
RefSeqiNP_564012.1. NM_101558.3.
UniGeneiAt.22247.

Genome annotation databases

EnsemblPlantsiAT1G16970.1; AT1G16970.1; AT1G16970.
GeneIDi838268.
GrameneiAT1G16970.1; AT1G16970.1; AT1G16970.
KEGGiath:AT1G16970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF283759 mRNA. Translation: AAG44852.1.
AC051629 Genomic DNA. Translation: AAF99835.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29527.1.
AK221642 mRNA. Translation: BAD95294.1.
PIRiD86305.
RefSeqiNP_564012.1. NM_101558.3.
UniGeneiAt.22247.

3D structure databases

ProteinModelPortaliQ9FQ08.
SMRiQ9FQ08. Positions 28-618.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23508. 3 interactions.
IntActiQ9FQ08. 3 interactions.
MINTiMINT-1212489.
STRINGi3702.AT1G16970.1.

PTM databases

iPTMnetiQ9FQ08.

Proteomic databases

PaxDbiQ9FQ08.
PRIDEiQ9FQ08.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G16970.1; AT1G16970.1; AT1G16970.
GeneIDi838268.
GrameneiAT1G16970.1; AT1G16970.1; AT1G16970.
KEGGiath:AT1G16970.

Organism-specific databases

TAIRiAT1G16970.

Phylogenomic databases

eggNOGiKOG2327. Eukaryota.
ENOG410XNXU. LUCA.
HOGENOMiHOG000030793.
InParanoidiQ9FQ08.
KOiK10884.
OMAiRKAYKFG.
PhylomeDBiQ9FQ08.

Enzyme and pathway databases

BioCyciARA:GQT-1986-MONOMER.

Miscellaneous databases

PROiQ9FQ08.

Gene expression databases

GenevisibleiQ9FQ08. AT.

Family and domain databases

Gene3Di1.10.1600.10. 1 hit.
1.10.720.30. 1 hit.
2.40.290.10. 1 hit.
3.40.50.410. 1 hit.
InterProiIPR006165. Ku70.
IPR006164. Ku70/Ku80_beta-barrel_dom.
IPR005160. Ku_C.
IPR005161. Ku_N.
IPR003034. SAP_dom.
IPR016194. SPOC-like_C_dom.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR12604:SF2. PTHR12604:SF2. 1 hit.
PfamiPF02735. Ku. 1 hit.
PF03730. Ku_C. 1 hit.
PF03731. Ku_N. 1 hit.
PF02037. SAP. 1 hit.
[Graphical view]
PIRSFiPIRSF003033. Ku70. 1 hit.
SMARTiSM00559. Ku78. 1 hit.
SM00513. SAP. 1 hit.
[Graphical view]
SUPFAMiSSF100939. SSF100939. 1 hit.
SSF53300. SSF53300. 1 hit.
TIGRFAMsiTIGR00578. ku70. 1 hit.
PROSITEiPS50800. SAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of Ku70 and Ku80 homologues in Arabidopsis thaliana: evidence for a role in the repair of DNA double-strand breaks."
    Tamura K., Adachi Y., Chiba K., Oguchi K., Takahashi H.
    Plant J. 29:771-781(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH KU80.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Telomere length deregulation and enhanced sensitivity to genotoxic stress in Arabidopsis mutants deficient in Ku70."
    Riha K., Watson J.M., Parkey J., Shippen D.E.
    EMBO J. 21:2819-2826(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH KU80, DISRUPTION PHENOTYPE.
  6. "Increased telomere length and hypersensitivity to DNA damaging agents in an Arabidopsis KU70 mutant."
    Bundock P., van Attikum H., Hooykaas P.
    Nucleic Acids Res. 30:3395-3400(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  7. "Disruption of the Arabidopsis AtKu80 gene demonstrates an essential role for AtKu80 protein in efficient repair of DNA double-strand breaks in vivo."
    West C.E., Waterworth W.M., Story G.W., Sunderland P.A., Jiang Q., Bray C.M.
    Plant J. 31:517-528(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH KU80.
  8. "Ku is required for telomeric C-rich strand maintenance but not for end-to-end chromosome fusions in Arabidopsis."
    Riha K., Shippen D.E.
    Proc. Natl. Acad. Sci. U.S.A. 100:611-615(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  9. "Interactions of putative telomere-binding proteins in Arabidopsis thaliana: identification of functional TRF2 homolog in plants."
    Kuchar M., Fajkus J.
    FEBS Lett. 578:311-315(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TRP1 AND KU80, MUTAGENESIS OF ARG-117 AND GLY-319.
  10. "A conserved and species-specific functional interaction between the Werner syndrome-like exonuclease atWEX and the Ku heterodimer in Arabidopsis."
    Li B., Conway N., Navarro S., Comai L., Comai L.
    Nucleic Acids Res. 33:6861-6867(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH WEX.
  11. "Regulation of Arabidopsis thaliana Ku genes at different developmental stages under heat stress."
    Liu P.F., Wang Y.K., Chang W.C., Chang H.Y., Pan R.L.
    Biochim. Biophys. Acta 1779:402-407(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY HEAT SHOCK.
  12. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
  13. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Ovate family protein 1 as a plant Ku70 interacting protein involving in DNA double-strand break repair."
    Wang Y.K., Chang W.C., Liu P.F., Hsiao M.K., Lin C.T., Lin S.M., Pan R.L.
    Plant Mol. Biol. 74:453-466(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH OFP1.

Entry informationi

Entry nameiKU70_ARATH
AccessioniPrimary (citable) accession number: Q9FQ08
Secondary accession number(s): Q9FZ56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: March 1, 2001
Last modified: February 17, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.