Q9FPW3 (DBBT_TAXCU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: 2-alpha-hydroxytaxane 2-O-benzoyltransferase Short name=TBT EC=2.3.1.166 Alternative name(s): 2-debenzoyl-7,13-diacetylbaccatin III-2-O-benzoyl transferase Short name=DBBT
Organism	Taxus cuspidata (Japanese yew)
Taxonomic identifier	99806 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Coniferophyta › Coniferopsida › Coniferales › Taxaceae › Taxus

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conversion of 2-debenzoyl-7,13-diacetylbaccatin III, a semisynthetic substrate, to 7,13-diacetylbaccatin III.
Catalytic activity	Benzoyl-CoA + 10-deacetyl-2-debenzoylbaccatin III = CoA + 10-deacetylbaccatin III.
Pathway	Alkaloid biosynthesis; taxol biosynthesis; baccatin III from 10-deacetyl-2-debenzoylbaccatin III: step 1/2.
Sequence similarities	Belongs to the plant acyltransferase family.

Ontologies

Keywords
Biological process	Taxol biosynthesis
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological_process	paclitaxel biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	2-alpha-hydroxytaxane 2-O-benzoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	2-alpha-hydroxytaxane 2-O-benzoyltransferase		PRO_0000147360

Sequences

	Sequence		Length	Mass (Da)
	Q9FPW3 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: A1EC9DB403A6005A	FASTA	440	50,089
	10 20 30 40 50 60 MGRFNVDMIE RVIVAPCLQS PKNILHLSPI DNKTRGLTNI LSVYNASQRV SVSADPAKTI 70 80 90 100 110 120 REALSKVLVY YPPFAGRLRN TENGDLEVEC TGEGAVFVEA MADNDLSVLQ DFNEYDPSFQ 130 140 150 160 170 180 QLVFNLREDV NIEDLHLLTV QVTRFTCGGF VVGTRFHHSV SDGKGIGQLL KGMGEMARGE 190 200 210 220 230 240 FKPSLEPIWN REMVKPEDIM YLQFDHFDFI HPPLNLEKSI QASMVISFER INYIKRCMME 250 260 270 280 290 300 ECKEFFSAFE VVVALIWLAR TKSFRIPPNE YVKIIFPIDM RNSFDSPLPK GYYGNAIGNA 310 320 330 340 350 360 CAMDNVKDLL NGSLLYALML IKKSKFALNE NFKSRILTKP STLDANMKHE NVVGCGDWRN 370 380 390 400 410 420 LGFYEADFGW GNAVNVSPMQ QQREHELAMQ NYFLFLRSAK NMIDGIKILM FMPASMVKPF 430 440 KIEMEVTINK YVAKICNSKL

References

[1]

"Taxol biosynthesis: molecular cloning of a benzoyl-CoA:taxane 2alpha-O-benzoyltransferase cDNA from Taxus and functional expression in Escherichia coli." Walker K., Croteau R.B. Proc. Natl. Acad. Sci. U.S.A. 97:13591-13596(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF297618 mRNA. Translation: AAG38049.1.
3D structure databases
ProteinModelPortal	Q9FPW3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13413.
BRENDA	2.3.1.166. 6225.
UniPathway	UPA00842; UER00810.
Family and domain databases
Gene3D	3.30.559.10. 2 hits.
InterPro	IPR023213. CAT-like_dom. IPR003480. Transferase. [Graphical view]
Pfam	PF02458. Transferase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DBBT_TAXCU

Accession

Primary (citable) accession number: Q9FPW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 22, 2003
Last sequence update:	March 1, 2001
Last modified:	April 3, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program