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Q9FPT5 (UBP1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 1

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 1
Short name=AtUBP1
Ubiquitin thioesterase 1
Ubiquitin-specific-processing protease 1
Gene names
Name:UBP1
Ordered Locus Names:At2g32780
ORF Names:F24L7.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length1083 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Is involved in resistance to the arginine analog canavanine (CAN). Ref.3

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 UBP-type zinc finger.

Contains 1 USP domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10831083Ubiquitin carboxyl-terminal hydrolase 1
PRO_0000080692

Regions

Domain202 – 1083882USP
Zinc finger79 – 14870UBP-type

Sites

Active site2111Nucleophile By similarity
Active site10291Proton acceptor By similarity

Experimental info

Mutagenesis2111C → S: Confers CAN sensitivity. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9FPT5 [UniParc].

Last modified April 26, 2005. Version 2.
Checksum: 55B881F9971199D9

FASTA1,083120,771
        10         20         30         40         50         60 
MRFAATVSEK QSEQSVEVTE EFAVTMTEKR SCVHFDKYVD LDKLLKIIKS YQQIKCGECN 

        70         80         90        100        110        120 
ERVHVKRGSR WGASNRRDWY SSSDQNCARN AIWLCLECGY YVCGDVGLPT EAQSHVMGHN 

       130        140        150        160        170        180 
RLTRHRLVIQ CKNPQLRWCF SCQSLLPFDN EENGEKKDLL LEVVKLIRER SPNTYSASFE 

       190        200        210        220        230        240 
TEFSCSGSIY GGIEARDGYA VRGLVNLGNT CFFNSVMQNL LSLDQLREHF LKEDLSVSGP 

       250        260        270        280        290        300 
LVSSLKELFA ESNSEASVFR NEINPRDLFF SVCSQAPQFR GYQQHDSHEL LRCLLDGLSI 

       310        320        330        340        350        360 
EESSLRKKLG VSDSNDSSTY QKPTLIDSVF GGEISSTVSC LECGHFSKVY EPFMDLSLPV 

       370        380        390        400        410        420 
PSMKLPPKKQ QILSQAKEVL KNGAVSKDSE VVSAKPASDH NSTVPLFPSD HKIQSRPETS 

       430        440        450        460        470        480 
DNETDLVLLS DVSDTAPSTE AKGVNQILVG STETLMHDND RTGKTVPDKE DVRATQSNEE 

       490        500        510        520        530        540 
TSASGISAVI DEAQVCGCPD LEQSSSSANQ GADEELALMV ADSQVLYMPY KDHLFYDDYM 

       550        560        570        580        590        600 
VAEASSSFVS GDHEPKKDYF DFSSFLDEPE IREGPVFRPL SKSEVYEAGF KADCSDDKTV 

       610        620        630        640        650        660 
SAGKGEASSS FISSDHEQNI DYVDFSSFFD EPEISERPFF RPLSKSEVSE AGFKADCSDD 

       670        680        690        700        710        720 
KTVSAGKGEA SSSFVSSDHE QNIDYVDFSS FFDEPEISER PFFRPLSKSE VSEAGFMAVS 

       730        740        750        760        770        780 
GNDKTVRAGK GETFSSFMSG DNERNIDYVE FTNRIFDDRG TSERPVFGPP SKAKVSEAGF 

       790        800        810        820        830        840 
VAVSSDSDPA VLDESDSPVS VDRCLAQFTK HEILSEDNAW HCENCSKNLK LQRLREKRRT 

       850        860        870        880        890        900 
KEGLSNRWVN ENGASSAFDE CRDSSLNQSC IDLENGYKAA PPITKLPNCK EEESAIDDGF 

       910        920        930        940        950        960 
VGEENTKQAP ITSVTETPLL GGETISSQPA SDNECENWED LAVDSEEVIV KRDARKKVLI 

       970        980        990       1000       1010       1020 
NKAPPVLTIH LKRFSQDARG RVSKLSGHVD FQEFIDLSKY MDTRCSEEDE PVYRLAGLVE 

      1030       1040       1050       1060       1070       1080 
HLGAMSRGHY VSYIRGGHKE RRDSDTKEPN SSIWYHASDS QVRPASLEEV LRSEAYILFY 


ERI 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 872-1025, GENE FAMILY ORGANIZATION, NOMENCLATURE, FUNCTION, MUTAGENESIS OF CYS-211.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC003974 Genomic DNA. Translation: AAC04485.1.
CP002685 Genomic DNA. Translation: AEC08739.1.
AF302658 mRNA. Translation: AAG42749.1.
PIRT00790.
RefSeqNP_565753.1. NM_128838.1.
UniGeneAt.38022.

3D structure databases

ProteinModelPortalQ9FPT5.
SMRQ9FPT5. Positions 91-364, 951-1080.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC19.091.

Proteomic databases

PRIDEQ9FPT5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G32780.1; AT2G32780.1; AT2G32780.
GeneID817839.
KEGGath:AT2G32780.

Organism-specific databases

TAIRAT2G32780.

Phylogenomic databases

eggNOGCOG5560.
HOGENOMHOG000090316.
InParanoidQ9FPT5.
KOK11844.
OMASDHEQNI.
PhylomeDBQ9FPT5.

Enzyme and pathway databases

BioCycARA:AT2G32780-MONOMER.

Gene expression databases

GenevestigatorQ9FPT5.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTSM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP1_ARATH
AccessionPrimary (citable) accession number: Q9FPT5
Secondary accession number(s): O48839
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: June 11, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names