Q9FPT5 (UBP1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 1
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 1
Short name=AtUBP1
Ubiquitin thioesterase 1
Ubiquitin-specific-processing protease 1

Gene names

Name:	UBP1
Ordered Locus Names:	At2g32780
ORF Names:	F24L7.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1083 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Is involved in resistance to the arginine analog canavanine (CAN). Ref.3
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C19 family. Contains 1 UBP-type zinc finger.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1083	1083	Ubiquitin carboxyl-terminal hydrolase 1		PRO_0000080692
Regions
	Zinc finger	79 – 148	70	UBP-type
Sites
	Active site	211	1	Nucleophile By similarity
	Active site	1029	1	Proton acceptor By similarity
Experimental info
	Mutagenesis	211	1	C → S: Confers CAN sensitivity. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9FPT5 [UniParc]. Last modified April 26, 2005. Version 2. Checksum: 55B881F9971199D9	FASTA	1,083	120,771
	10 20 30 40 50 60 MRFAATVSEK QSEQSVEVTE EFAVTMTEKR SCVHFDKYVD LDKLLKIIKS YQQIKCGECN 70 80 90 100 110 120 ERVHVKRGSR WGASNRRDWY SSSDQNCARN AIWLCLECGY YVCGDVGLPT EAQSHVMGHN 130 140 150 160 170 180 RLTRHRLVIQ CKNPQLRWCF SCQSLLPFDN EENGEKKDLL LEVVKLIRER SPNTYSASFE 190 200 210 220 230 240 TEFSCSGSIY GGIEARDGYA VRGLVNLGNT CFFNSVMQNL LSLDQLREHF LKEDLSVSGP 250 260 270 280 290 300 LVSSLKELFA ESNSEASVFR NEINPRDLFF SVCSQAPQFR GYQQHDSHEL LRCLLDGLSI 310 320 330 340 350 360 EESSLRKKLG VSDSNDSSTY QKPTLIDSVF GGEISSTVSC LECGHFSKVY EPFMDLSLPV 370 380 390 400 410 420 PSMKLPPKKQ QILSQAKEVL KNGAVSKDSE VVSAKPASDH NSTVPLFPSD HKIQSRPETS 430 440 450 460 470 480 DNETDLVLLS DVSDTAPSTE AKGVNQILVG STETLMHDND RTGKTVPDKE DVRATQSNEE 490 500 510 520 530 540 TSASGISAVI DEAQVCGCPD LEQSSSSANQ GADEELALMV ADSQVLYMPY KDHLFYDDYM 550 560 570 580 590 600 VAEASSSFVS GDHEPKKDYF DFSSFLDEPE IREGPVFRPL SKSEVYEAGF KADCSDDKTV 610 620 630 640 650 660 SAGKGEASSS FISSDHEQNI DYVDFSSFFD EPEISERPFF RPLSKSEVSE AGFKADCSDD 670 680 690 700 710 720 KTVSAGKGEA SSSFVSSDHE QNIDYVDFSS FFDEPEISER PFFRPLSKSE VSEAGFMAVS 730 740 750 760 770 780 GNDKTVRAGK GETFSSFMSG DNERNIDYVE FTNRIFDDRG TSERPVFGPP SKAKVSEAGF 790 800 810 820 830 840 VAVSSDSDPA VLDESDSPVS VDRCLAQFTK HEILSEDNAW HCENCSKNLK LQRLREKRRT 850 860 870 880 890 900 KEGLSNRWVN ENGASSAFDE CRDSSLNQSC IDLENGYKAA PPITKLPNCK EEESAIDDGF 910 920 930 940 950 960 VGEENTKQAP ITSVTETPLL GGETISSQPA SDNECENWED LAVDSEEVIV KRDARKKVLI 970 980 990 1000 1010 1020 NKAPPVLTIH LKRFSQDARG RVSKLSGHVD FQEFIDLSKY MDTRCSEEDE PVYRLAGLVE 1030 1040 1050 1060 1070 1080 HLGAMSRGHY VSYIRGGHKE RRDSDTKEPN SSIWYHASDS QVRPASLEEV LRSEAYILFY ERI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine." Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D. Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 872-1025, GENE FAMILY ORGANIZATION, NOMENCLATURE, FUNCTION, MUTAGENESIS OF CYS-211. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC003974 Genomic DNA. Translation: AAC04485.1. CP002685 Genomic DNA. Translation: AEC08739.1. AF302658 mRNA. Translation: AAG42749.1.
IPI	IPI00548853.
PIR	T00790.
RefSeq	NP_565753.1. NM_128838.1.
UniGene	At.38022.
3D structure databases
ProteinModelPortal	Q9FPT5.
SMR	Q9FPT5. Positions 91-364, 951-1080.
ModBase	Search...
Protein family/group databases
MEROPS	C19.091.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G32780.1; AT2G32780.1; AT2G32780.
GeneID	817839.
KEGG	ath:AT2G32780.
Organism-specific databases
TAIR	At2g32780.
Phylogenomic databases
eggNOG	COG5560.
HOGENOM	HOG000090316.
InParanoid	Q9FPT5.
KO	K11844.
OMA	FFDEPEI.
PhylomeDB	Q9FPT5.
ProtClustDB	CLSN2917217.
Gene expression databases
Genevestigator	Q9FPT5.
GermOnline	AT2G32780. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR013083. Znf_RING/FYVE/PHD. IPR001607. Znf_UBP. [Graphical view]
Pfam	PF00443. UCH. 1 hit. PF02148. zf-UBP. 1 hit. [Graphical view]
SMART	SM00290. ZnF_UBP. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. PS50271. ZF_UBP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP1_ARATH

Accession

Primary (citable) accession number: Q9FPT5
Secondary accession number(s): O48839

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	April 26, 2005
Last modified:	May 1, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program