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Protein

Ubiquitin carboxyl-terminal hydrolase 1

Gene

UBP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Is involved in resistance to the arginine analog canavanine (CAN).1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei211 – 2111NucleophilePROSITE-ProRule annotation
Active sitei1029 – 10291Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri79 – 14870UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: GO_Central
  2. ubiquitin-specific protease activity Source: GO_Central
  3. ubiquitin thiolesterase activity Source: GO_Central
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  2. protein deubiquitination Source: GO_Central
  3. regulation of proteasomal protein catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT2G32780-MONOMER.

Protein family/group databases

MEROPSiC19.091.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 1 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 1
Short name:
AtUBP1
Ubiquitin thioesterase 1
Ubiquitin-specific-processing protease 1
Gene namesi
Name:UBP1
Ordered Locus Names:At2g32780
ORF Names:F24L7.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G32780.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi211 – 2111C → S: Confers CAN sensitivity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10831083Ubiquitin carboxyl-terminal hydrolase 1PRO_0000080692Add
BLAST

Proteomic databases

PRIDEiQ9FPT5.

Expressioni

Gene expression databases

GenevestigatoriQ9FPT5.

Structurei

3D structure databases

ProteinModelPortaliQ9FPT5.
SMRiQ9FPT5. Positions 91-364, 951-1080.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini202 – 1083882USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri79 – 14870UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5560.
HOGENOMiHOG000090316.
InParanoidiQ9FPT5.
KOiK11844.
OMAiSDHEQNI.
PhylomeDBiQ9FPT5.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FPT5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRFAATVSEK QSEQSVEVTE EFAVTMTEKR SCVHFDKYVD LDKLLKIIKS
60 70 80 90 100
YQQIKCGECN ERVHVKRGSR WGASNRRDWY SSSDQNCARN AIWLCLECGY
110 120 130 140 150
YVCGDVGLPT EAQSHVMGHN RLTRHRLVIQ CKNPQLRWCF SCQSLLPFDN
160 170 180 190 200
EENGEKKDLL LEVVKLIRER SPNTYSASFE TEFSCSGSIY GGIEARDGYA
210 220 230 240 250
VRGLVNLGNT CFFNSVMQNL LSLDQLREHF LKEDLSVSGP LVSSLKELFA
260 270 280 290 300
ESNSEASVFR NEINPRDLFF SVCSQAPQFR GYQQHDSHEL LRCLLDGLSI
310 320 330 340 350
EESSLRKKLG VSDSNDSSTY QKPTLIDSVF GGEISSTVSC LECGHFSKVY
360 370 380 390 400
EPFMDLSLPV PSMKLPPKKQ QILSQAKEVL KNGAVSKDSE VVSAKPASDH
410 420 430 440 450
NSTVPLFPSD HKIQSRPETS DNETDLVLLS DVSDTAPSTE AKGVNQILVG
460 470 480 490 500
STETLMHDND RTGKTVPDKE DVRATQSNEE TSASGISAVI DEAQVCGCPD
510 520 530 540 550
LEQSSSSANQ GADEELALMV ADSQVLYMPY KDHLFYDDYM VAEASSSFVS
560 570 580 590 600
GDHEPKKDYF DFSSFLDEPE IREGPVFRPL SKSEVYEAGF KADCSDDKTV
610 620 630 640 650
SAGKGEASSS FISSDHEQNI DYVDFSSFFD EPEISERPFF RPLSKSEVSE
660 670 680 690 700
AGFKADCSDD KTVSAGKGEA SSSFVSSDHE QNIDYVDFSS FFDEPEISER
710 720 730 740 750
PFFRPLSKSE VSEAGFMAVS GNDKTVRAGK GETFSSFMSG DNERNIDYVE
760 770 780 790 800
FTNRIFDDRG TSERPVFGPP SKAKVSEAGF VAVSSDSDPA VLDESDSPVS
810 820 830 840 850
VDRCLAQFTK HEILSEDNAW HCENCSKNLK LQRLREKRRT KEGLSNRWVN
860 870 880 890 900
ENGASSAFDE CRDSSLNQSC IDLENGYKAA PPITKLPNCK EEESAIDDGF
910 920 930 940 950
VGEENTKQAP ITSVTETPLL GGETISSQPA SDNECENWED LAVDSEEVIV
960 970 980 990 1000
KRDARKKVLI NKAPPVLTIH LKRFSQDARG RVSKLSGHVD FQEFIDLSKY
1010 1020 1030 1040 1050
MDTRCSEEDE PVYRLAGLVE HLGAMSRGHY VSYIRGGHKE RRDSDTKEPN
1060 1070 1080
SSIWYHASDS QVRPASLEEV LRSEAYILFY ERI
Length:1,083
Mass (Da):120,771
Last modified:April 26, 2005 - v2
Checksum:i55B881F9971199D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003974 Genomic DNA. Translation: AAC04485.1.
CP002685 Genomic DNA. Translation: AEC08739.1.
AF302658 mRNA. Translation: AAG42749.1.
PIRiT00790.
RefSeqiNP_565753.1. NM_128838.1.
UniGeneiAt.38022.

Genome annotation databases

EnsemblPlantsiAT2G32780.1; AT2G32780.1; AT2G32780.
GeneIDi817839.
KEGGiath:AT2G32780.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003974 Genomic DNA. Translation: AAC04485.1.
CP002685 Genomic DNA. Translation: AEC08739.1.
AF302658 mRNA. Translation: AAG42749.1.
PIRiT00790.
RefSeqiNP_565753.1. NM_128838.1.
UniGeneiAt.38022.

3D structure databases

ProteinModelPortaliQ9FPT5.
SMRiQ9FPT5. Positions 91-364, 951-1080.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC19.091.

Proteomic databases

PRIDEiQ9FPT5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G32780.1; AT2G32780.1; AT2G32780.
GeneIDi817839.
KEGGiath:AT2G32780.

Organism-specific databases

TAIRiAT2G32780.

Phylogenomic databases

eggNOGiCOG5560.
HOGENOMiHOG000090316.
InParanoidiQ9FPT5.
KOiK11844.
OMAiSDHEQNI.
PhylomeDBiQ9FPT5.

Enzyme and pathway databases

BioCyciARA:AT2G32780-MONOMER.

Gene expression databases

GenevestigatoriQ9FPT5.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00290. ZnF_UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
    Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
    Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 872-1025, GENE FAMILY ORGANIZATION, NOMENCLATURE, FUNCTION, MUTAGENESIS OF CYS-211.
    Strain: cv. Columbia.

Entry informationi

Entry nameiUBP1_ARATH
AccessioniPrimary (citable) accession number: Q9FPT5
Secondary accession number(s): O48839
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: February 4, 2015
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.