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Q9FPT5

- UBP1_ARATH

UniProt

Q9FPT5 - UBP1_ARATH

Protein

Ubiquitin carboxyl-terminal hydrolase 1

Gene

UBP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 2 (26 Apr 2005)
      Previous versions | rss
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    Functioni

    Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Is involved in resistance to the arginine analog canavanine (CAN).1 Publication

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei211 – 2111NucleophilePROSITE-ProRule annotation
    Active sitei1029 – 10291Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri79 – 14870UBP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitinyl hydrolase activity Source: InterPro
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:AT2G32780-MONOMER.

    Protein family/group databases

    MEROPSiC19.091.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 1 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 1
    Short name:
    AtUBP1
    Ubiquitin thioesterase 1
    Ubiquitin-specific-processing protease 1
    Gene namesi
    Name:UBP1
    Ordered Locus Names:At2g32780
    ORF Names:F24L7.8
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G32780.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi211 – 2111C → S: Confers CAN sensitivity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10831083Ubiquitin carboxyl-terminal hydrolase 1PRO_0000080692Add
    BLAST

    Proteomic databases

    PRIDEiQ9FPT5.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9FPT5.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FPT5.
    SMRiQ9FPT5. Positions 91-364, 951-1080.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini202 – 1083882USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri79 – 14870UBP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5560.
    HOGENOMiHOG000090316.
    InParanoidiQ9FPT5.
    KOiK11844.
    OMAiSDHEQNI.
    PhylomeDBiQ9FPT5.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    SMARTiSM00290. ZnF_UBP. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9FPT5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRFAATVSEK QSEQSVEVTE EFAVTMTEKR SCVHFDKYVD LDKLLKIIKS     50
    YQQIKCGECN ERVHVKRGSR WGASNRRDWY SSSDQNCARN AIWLCLECGY 100
    YVCGDVGLPT EAQSHVMGHN RLTRHRLVIQ CKNPQLRWCF SCQSLLPFDN 150
    EENGEKKDLL LEVVKLIRER SPNTYSASFE TEFSCSGSIY GGIEARDGYA 200
    VRGLVNLGNT CFFNSVMQNL LSLDQLREHF LKEDLSVSGP LVSSLKELFA 250
    ESNSEASVFR NEINPRDLFF SVCSQAPQFR GYQQHDSHEL LRCLLDGLSI 300
    EESSLRKKLG VSDSNDSSTY QKPTLIDSVF GGEISSTVSC LECGHFSKVY 350
    EPFMDLSLPV PSMKLPPKKQ QILSQAKEVL KNGAVSKDSE VVSAKPASDH 400
    NSTVPLFPSD HKIQSRPETS DNETDLVLLS DVSDTAPSTE AKGVNQILVG 450
    STETLMHDND RTGKTVPDKE DVRATQSNEE TSASGISAVI DEAQVCGCPD 500
    LEQSSSSANQ GADEELALMV ADSQVLYMPY KDHLFYDDYM VAEASSSFVS 550
    GDHEPKKDYF DFSSFLDEPE IREGPVFRPL SKSEVYEAGF KADCSDDKTV 600
    SAGKGEASSS FISSDHEQNI DYVDFSSFFD EPEISERPFF RPLSKSEVSE 650
    AGFKADCSDD KTVSAGKGEA SSSFVSSDHE QNIDYVDFSS FFDEPEISER 700
    PFFRPLSKSE VSEAGFMAVS GNDKTVRAGK GETFSSFMSG DNERNIDYVE 750
    FTNRIFDDRG TSERPVFGPP SKAKVSEAGF VAVSSDSDPA VLDESDSPVS 800
    VDRCLAQFTK HEILSEDNAW HCENCSKNLK LQRLREKRRT KEGLSNRWVN 850
    ENGASSAFDE CRDSSLNQSC IDLENGYKAA PPITKLPNCK EEESAIDDGF 900
    VGEENTKQAP ITSVTETPLL GGETISSQPA SDNECENWED LAVDSEEVIV 950
    KRDARKKVLI NKAPPVLTIH LKRFSQDARG RVSKLSGHVD FQEFIDLSKY 1000
    MDTRCSEEDE PVYRLAGLVE HLGAMSRGHY VSYIRGGHKE RRDSDTKEPN 1050
    SSIWYHASDS QVRPASLEEV LRSEAYILFY ERI 1083
    Length:1,083
    Mass (Da):120,771
    Last modified:April 26, 2005 - v2
    Checksum:i55B881F9971199D9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC003974 Genomic DNA. Translation: AAC04485.1.
    CP002685 Genomic DNA. Translation: AEC08739.1.
    AF302658 mRNA. Translation: AAG42749.1.
    PIRiT00790.
    RefSeqiNP_565753.1. NM_128838.1.
    UniGeneiAt.38022.

    Genome annotation databases

    EnsemblPlantsiAT2G32780.1; AT2G32780.1; AT2G32780.
    GeneIDi817839.
    KEGGiath:AT2G32780.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC003974 Genomic DNA. Translation: AAC04485.1 .
    CP002685 Genomic DNA. Translation: AEC08739.1 .
    AF302658 mRNA. Translation: AAG42749.1 .
    PIRi T00790.
    RefSeqi NP_565753.1. NM_128838.1.
    UniGenei At.38022.

    3D structure databases

    ProteinModelPortali Q9FPT5.
    SMRi Q9FPT5. Positions 91-364, 951-1080.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C19.091.

    Proteomic databases

    PRIDEi Q9FPT5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G32780.1 ; AT2G32780.1 ; AT2G32780 .
    GeneIDi 817839.
    KEGGi ath:AT2G32780.

    Organism-specific databases

    TAIRi AT2G32780.

    Phylogenomic databases

    eggNOGi COG5560.
    HOGENOMi HOG000090316.
    InParanoidi Q9FPT5.
    KOi K11844.
    OMAi SDHEQNI.
    PhylomeDBi Q9FPT5.

    Enzyme and pathway databases

    BioCyci ARA:AT2G32780-MONOMER.

    Gene expression databases

    Genevestigatori Q9FPT5.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    SMARTi SM00290. ZnF_UBP. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
      Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
      Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 872-1025, GENE FAMILY ORGANIZATION, NOMENCLATURE, FUNCTION, MUTAGENESIS OF CYS-211.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiUBP1_ARATH
    AccessioniPrimary (citable) accession number: Q9FPT5
    Secondary accession number(s): O48839
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 84 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3