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Protein

Ubiquitin carboxyl-terminal hydrolase 12

Gene

UBP12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei208NucleophilePROSITE-ProRule annotation1
Active sitei455Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • thiol-dependent ubiquitin-specific protease activity Source: TAIR

GO - Biological processi

  • jasmonic acid mediated signaling pathway Source: TAIR
  • protein deubiquitination Source: InterPro
  • ubiquitin-dependent protein catabolic process Source: InterPro

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-ATH-6781823 Formation of TC-NER Pre-Incision Complex
R-ATH-6782135 Dual incision in TC-NER
R-ATH-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-ATH-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes

Protein family/group databases

MEROPSiC19.A73

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 12 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 12
Short name:
AtUBP12
Ubiquitin thioesterase 12
Ubiquitin-specific-processing protease 12
Gene namesi
Name:UBP12
Ordered Locus Names:At5g06600
ORF Names:F15M7.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G06600
TAIRilocus:2144118 AT5G06600

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003130391 – 1116Ubiquitin carboxyl-terminal hydrolase 12Add BLAST1116

Proteomic databases

PaxDbiQ9FPT1
PRIDEiQ9FPT1

PTM databases

iPTMnetiQ9FPT1

Expressioni

Gene expression databases

ExpressionAtlasiQ9FPT1 baseline and differential
GenevisibleiQ9FPT1 AT

Interactioni

Subunit structurei

Interacts with SIC/RON3.1 Publication

Protein-protein interaction databases

BioGridi15827, 4 interactors
STRINGi3702.AT5G06600.1

Structurei

3D structure databases

ProteinModelPortaliQ9FPT1
SMRiQ9FPT1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 179MATHPROSITE-ProRule annotationAdd BLAST126
Domaini199 – 524USPAdd BLAST326

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Phylogenomic databases

eggNOGiKOG1863 Eukaryota
COG5077 LUCA
HOGENOMiHOG000160240
InParanoidiQ9FPT1
KOiK11838
OMAiINAKKHY
OrthoDBiEOG093600LZ
PhylomeDBiQ9FPT1

Family and domain databases

CDDicd00121 MATH, 1 hit
Gene3Di2.60.210.10, 1 hit
InterProiView protein in InterPro
IPR002083 MATH/TRAF_dom
IPR001394 Peptidase_C19_UCH
IPR008974 TRAF-like
IPR024729 USP7_ICP0-binding_dom
IPR029346 USP_C
IPR018200 USP_CS
IPR028889 USP_dom
PfamiView protein in Pfam
PF00917 MATH, 1 hit
PF00443 UCH, 1 hit
PF14533 USP7_C2, 1 hit
PF12436 USP7_ICP0_bdg, 1 hit
SMARTiView protein in SMART
SM00061 MATH, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS50144 MATH, 1 hit
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9FPT1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTMMTPPPVD QPEDEEMLVP NSDLVDGPAQ PMEVTQPETA ASTVENQPAE
60 70 80 90 100
DPPTLKFTWT IPNFSRQNTR KHYSDVFVVG GYKWRILIFP KGNNVDHLSM
110 120 130 140 150
YLDVSDAASL PYGWSRYAQF SLAVVNQIHT RYTVRKETQH QFNARESDWG
160 170 180 190 200
FTSFMPLSEL YDPSRGYLVN DTVLVEAEVA VRKVLDYWSY DSKKETGFVG
210 220 230 240 250
LKNQGATCYM NSLLQTLYHI PYFRKAVYHM PTTENDAPTA SIPLALQSLF
260 270 280 290 300
YKLQYNDTSV ATKELTKSFG WDTYDSFMQH DVQELNRVLC EKLEDKMKGT
310 320 330 340 350
VVEGTIQQLF EGHHMNYIEC INVDFKSTRK ESFYDLQLDV KGCKDVYASF
360 370 380 390 400
DKYVEVERLE GDNKYHAEGH GLQDAKKGVL FIDFPPVLQL QLKRFEYDFM
410 420 430 440 450
RDTMVKINDR YEFPLELDLD REDGKYLSPD ADRSVRNLYT LHSVLVHSGG
460 470 480 490 500
VHGGHYYAFI RPTLSDQWYK FDDERVTKED LKRALEEQYG GEEELPQTNP
510 520 530 540 550
GFNNNPPFKF TKYSNAYMLV YIRESDKDKI ICNVDEKDIA EHLRVRLKKE
560 570 580 590 600
QEEKEDKRRY KAQAHLYTII KVARDEDLKE QIGKDIYFDL VDHDKVRSFR
610 620 630 640 650
IQKQTPFQQF KEEVAKEFGV PVQLQRFWIW AKRQNHTYRP NRPLTPQEEL
660 670 680 690 700
QPVGQIREAS NKANTAELKL FLEVEHLDLR PIPPPEKSKE DILLFFKLYD
710 720 730 740 750
PEKAVLSYAG RLMVKSSSKP MDITGKLNEM VGFAPDEEIE LFEEIKFEPC
760 770 780 790 800
VMCEHLDKKT SFRLCQIEDG DIICFQKPLV NKEIECLYPA VPSFLEYVQN
810 820 830 840 850
RQLVRFRALE KPKEDEFVLE LSKQHTYDDV VEKVAEKLGL DDPSKLRLTS
860 870 880 890 900
HNCYSQQPKP QPIKYRGVDH LSDMLVHYNQ TSDILYYEVL DIPLPELQGL
910 920 930 940 950
KTLKVAFHHA TKEEVVIHNI RLPKQSTVGD VINELKTKVE LSHPDAELRL
960 970 980 990 1000
LEVFYHKIYK IFPSTERIEN INDQYWTLRA EEIPEEEKNI GPNDRLILVY
1010 1020 1030 1040 1050
HFAKETGQNQ QVQNFGEPFF LVIHEGETLE EIKNRIQKKL HVSDEDFAKW
1060 1070 1080 1090 1100
KFAFMSMGRP EYLQDTDVVY NRFQRRDVYG AFEQYLGLEH ADTTPKRAYA
1110
ANQNRHAYEK PVKIYN
Length:1,116
Mass (Da):130,607
Last modified:January 15, 2008 - v2
Checksum:iE1FD0B84E6D5EC68
GO
Isoform 2 (identifier: Q9FPT1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     11-11: Missing.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.
Show »
Length:1,115
Mass (Da):130,479
Checksum:i4AB4E10D836C2172
GO

Sequence cautioni

The sequence BAB11409 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti840L → F in AAG42754 (PubMed:11115897).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02998911Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302663 mRNA Translation: AAG42754.1
AP002543 Genomic DNA Translation: BAB11409.1 Sequence problems.
CP002688 Genomic DNA Translation: AED91039.1
CP002688 Genomic DNA Translation: AED91040.1
AF360198 mRNA Translation: AAK25908.1
AY142616 mRNA Translation: AAN13185.1
RefSeqiNP_568171.1, NM_120743.3 [Q9FPT1-1]
NP_850783.1, NM_180452.1 [Q9FPT1-2]
UniGeneiAt.22362
At.67352

Genome annotation databases

EnsemblPlantsiAT5G06600.1; AT5G06600.1; AT5G06600 [Q9FPT1-1]
AT5G06600.2; AT5G06600.2; AT5G06600 [Q9FPT1-2]
GeneIDi830548
GrameneiAT5G06600.1; AT5G06600.1; AT5G06600 [Q9FPT1-1]
AT5G06600.2; AT5G06600.2; AT5G06600 [Q9FPT1-2]
KEGGiath:AT5G06600

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBP12_ARATH
AccessioniPrimary (citable) accession number: Q9FPT1
Secondary accession number(s): Q9C5K1, Q9FG10
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: May 23, 2018
This is version 115 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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