Q9FPT1 (UBP12_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 73.
History...
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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Ubiquitin carboxyl-terminal hydrolase 12 EC=3.4.19.12 Alternative name(s): Deubiquitinating enzyme 12 Short name=AtUBP12 Ubiquitin thioesterase 12 Ubiquitin-specific-processing protease 12 | ||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Reference proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Protein attributes
| Sequence length | 1116 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity. |
| Catalytic activity | Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). |
| Post-translational modification | Ubiquitinated. |
| Sequence similarities | Belongs to the peptidase C19 family. Contains 1 MATH domain. |
| Sequence caution | The sequence BAB11409.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Ubl conjugation pathway |
| Coding sequence diversity | Alternative splicing |
| Molecular function | Hydrolase Protease Thiol protease |
| PTM | Ubl conjugation |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro |
| Cellular_component | cytosol Inferred from direct assay PubMed 21166475. Source: TAIR |
| Molecular_function | cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q9FPT1-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q9FPT1-2) The sequence of this isoform differs from the canonical sequence as follows: 11-11: Missing. | ||||||
| Note: May be due to a competing acceptor splice site. No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1116 | 1116 | Ubiquitin carboxyl-terminal hydrolase 12 | PRO_0000313039 | |||||
Regions | |||||||||
| Domain | 54 – 179 | 126 | MATH | ||||||
Sites | |||||||||
| Active site | 208 | 1 | Nucleophile By similarity | ||||||
| Active site | 455 | 1 | Proton acceptor By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 11 | 1 | Missing in isoform 2. | VSP_029989 | |||||
Experimental info | |||||||||
| Sequence conflict | 840 | 1 | L → F in AAG42754. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine." Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D. Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY ORGANIZATION, NOMENCLATURE. Strain: cv. Columbia. |
| [2] | "Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia. |
| [4] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.  Ecker J.R.Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia. |
| [5] | "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis." Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D. Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF302663 mRNA. Translation: AAG42754.1. AP002543 Genomic DNA. Translation: BAB11409.1. Sequence problems. CP002688 Genomic DNA. Translation: AED91039.1. CP002688 Genomic DNA. Translation: AED91040.1. AF360198 mRNA. Translation: AAK25908.1. AY142616 mRNA. Translation: AAN13185.1. |
| IPI | IPI00540940. IPI00547717. |
| RefSeq | NP_568171.1. NM_120743.2. NP_850783.1. NM_180452.1. |
| UniGene | At.22362. At.67352. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1NBF based on UniProtKB Q93009. |
| ProteinModelPortal | Q9FPT1. |
| SMR | Q9FPT1. Positions 57-640. |
| ModBase | Search... |
Proteomic databases | |
| PaxDb | Q9FPT1. |
| PRIDE | Q9FPT1. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | AT5G06600.1; AT5G06600.1; AT5G06600. |
| GeneID | 830548. |
| KEGG | ath:AT5G06600. |
Organism-specific databases | |
| TAIR | At5g06600. |
Phylogenomic databases | |
| eggNOG | COG5077. |
| HOGENOM | HOG000160240. |
| InParanoid | Q9FPT1. |
| KO | K11838. |
| OMA | RLFVKSS. |
| PhylomeDB | Q9FPT1. |
| ProtClustDB | CLSN2689510. |
Gene expression databases | |
| ArrayExpress | Q9FPT1. |
| Genevestigator | Q9FPT1. |
Family and domain databases | |
| InterPro | IPR002083. MATH. IPR024729. Pept_C19_dom. IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR008974. TRAF-like. [Graphical view] |
| Pfam | PF00917. MATH. 1 hit. PF00443. UCH. 1 hit. PF12436. USP7. 1 hit. [Graphical view] |
| SMART | SM00061. MATH. 1 hit. [Graphical view] |
| SUPFAM | SSF49599. Traf_like. 1 hit. |
| PROSITE | PS50144. MATH. 1 hit. PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | UBP12_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q9FPT1 Secondary accession number(s): Q9C5K1, Q9FG10 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |