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Q9FPT1 (UBP12_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 12

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 12
Short name=AtUBP12
Ubiquitin thioesterase 12
Ubiquitin-specific-processing protease 12
Gene names
Name:UBP12
Ordered Locus Names:At5g06600
ORF Names:F15M7.13
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length1116 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Post-translational modification

Ubiquitinated.

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 MATH domain.

Contains 1 USP domain.

Sequence caution

The sequence BAB11409.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Coding sequence diversityAlternative splicing
   Molecular functionHydrolase
Protease
Thiol protease
   PTMUbl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytosol

Inferred from direct assay PubMed 21166475. Source: TAIR

   Molecular_functioncysteine-type peptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9FPT1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9FPT1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     11-11: Missing.
Note: May be due to a competing acceptor splice site. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11161116Ubiquitin carboxyl-terminal hydrolase 12
PRO_0000313039

Regions

Domain54 – 179126MATH
Domain199 – 524326USP

Sites

Active site2081Nucleophile By similarity
Active site4551Proton acceptor By similarity

Natural variations

Alternative sequence111Missing in isoform 2.
VSP_029989

Experimental info

Sequence conflict8401L → F in AAG42754. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 15, 2008. Version 2.
Checksum: E1FD0B84E6D5EC68

FASTA1,116130,607
        10         20         30         40         50         60 
MTMMTPPPVD QPEDEEMLVP NSDLVDGPAQ PMEVTQPETA ASTVENQPAE DPPTLKFTWT 

        70         80         90        100        110        120 
IPNFSRQNTR KHYSDVFVVG GYKWRILIFP KGNNVDHLSM YLDVSDAASL PYGWSRYAQF 

       130        140        150        160        170        180 
SLAVVNQIHT RYTVRKETQH QFNARESDWG FTSFMPLSEL YDPSRGYLVN DTVLVEAEVA 

       190        200        210        220        230        240 
VRKVLDYWSY DSKKETGFVG LKNQGATCYM NSLLQTLYHI PYFRKAVYHM PTTENDAPTA 

       250        260        270        280        290        300 
SIPLALQSLF YKLQYNDTSV ATKELTKSFG WDTYDSFMQH DVQELNRVLC EKLEDKMKGT 

       310        320        330        340        350        360 
VVEGTIQQLF EGHHMNYIEC INVDFKSTRK ESFYDLQLDV KGCKDVYASF DKYVEVERLE 

       370        380        390        400        410        420 
GDNKYHAEGH GLQDAKKGVL FIDFPPVLQL QLKRFEYDFM RDTMVKINDR YEFPLELDLD 

       430        440        450        460        470        480 
REDGKYLSPD ADRSVRNLYT LHSVLVHSGG VHGGHYYAFI RPTLSDQWYK FDDERVTKED 

       490        500        510        520        530        540 
LKRALEEQYG GEEELPQTNP GFNNNPPFKF TKYSNAYMLV YIRESDKDKI ICNVDEKDIA 

       550        560        570        580        590        600 
EHLRVRLKKE QEEKEDKRRY KAQAHLYTII KVARDEDLKE QIGKDIYFDL VDHDKVRSFR 

       610        620        630        640        650        660 
IQKQTPFQQF KEEVAKEFGV PVQLQRFWIW AKRQNHTYRP NRPLTPQEEL QPVGQIREAS 

       670        680        690        700        710        720 
NKANTAELKL FLEVEHLDLR PIPPPEKSKE DILLFFKLYD PEKAVLSYAG RLMVKSSSKP 

       730        740        750        760        770        780 
MDITGKLNEM VGFAPDEEIE LFEEIKFEPC VMCEHLDKKT SFRLCQIEDG DIICFQKPLV 

       790        800        810        820        830        840 
NKEIECLYPA VPSFLEYVQN RQLVRFRALE KPKEDEFVLE LSKQHTYDDV VEKVAEKLGL 

       850        860        870        880        890        900 
DDPSKLRLTS HNCYSQQPKP QPIKYRGVDH LSDMLVHYNQ TSDILYYEVL DIPLPELQGL 

       910        920        930        940        950        960 
KTLKVAFHHA TKEEVVIHNI RLPKQSTVGD VINELKTKVE LSHPDAELRL LEVFYHKIYK 

       970        980        990       1000       1010       1020 
IFPSTERIEN INDQYWTLRA EEIPEEEKNI GPNDRLILVY HFAKETGQNQ QVQNFGEPFF 

      1030       1040       1050       1060       1070       1080 
LVIHEGETLE EIKNRIQKKL HVSDEDFAKW KFAFMSMGRP EYLQDTDVVY NRFQRRDVYG 

      1090       1100       1110 
AFEQYLGLEH ADTTPKRAYA ANQNRHAYEK PVKIYN 

« Hide

Isoform 2 [UniParc].

Checksum: 4AB4E10D836C2172
Show »

FASTA1,115130,479

References

« Hide 'large scale' references
[1]"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY ORGANIZATION, NOMENCLATURE.
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. XI."
Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[5]"Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF302663 mRNA. Translation: AAG42754.1.
AP002543 Genomic DNA. Translation: BAB11409.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED91039.1.
CP002688 Genomic DNA. Translation: AED91040.1.
AF360198 mRNA. Translation: AAK25908.1.
AY142616 mRNA. Translation: AAN13185.1.
RefSeqNP_568171.1. NM_120743.2.
NP_850783.1. NM_180452.1.
UniGeneAt.22362.
At.67352.

3D structure databases

ProteinModelPortalQ9FPT1.
SMRQ9FPT1. Positions 57-674.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid15827. 4 interactions.

Protein family/group databases

MEROPSC19.A73.

Proteomic databases

PaxDbQ9FPT1.
PRIDEQ9FPT1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G06600.1; AT5G06600.1; AT5G06600. [Q9FPT1-1]
GeneID830548.
KEGGath:AT5G06600.

Organism-specific databases

TAIRAT5G06600.

Phylogenomic databases

eggNOGCOG5077.
HOGENOMHOG000160240.
InParanoidQ9FPT1.
KOK11838.
OMAYKLQDAN.
PhylomeDBQ9FPT1.
ProtClustDBCLSN2689510.

Enzyme and pathway databases

BioCycARA:AT5G06600-MONOMER.
ARA:GQT-714-MONOMER.
ARA:GQT-715-MONOMER.

Gene expression databases

GenevestigatorQ9FPT1.

Family and domain databases

InterProIPR002083. MATH.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR008974. TRAF-like.
IPR028889. UCH/PAN2.
IPR024729. USP7_ICP0-binding_dom.
[Graphical view]
PfamPF00917. MATH. 1 hit.
PF00443. UCH. 1 hit.
PF12436. USP7_ICP0_bdg. 1 hit.
[Graphical view]
SMARTSM00061. MATH. 1 hit.
[Graphical view]
SUPFAMSSF49599. SSF49599. 1 hit.
PROSITEPS50144. MATH. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP12_ARATH
AccessionPrimary (citable) accession number: Q9FPT1
Secondary accession number(s): Q9C5K1, Q9FG10
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: April 16, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names