Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9FPS9

- UBP15_ARATH

UniProt

Q9FPS9 - UBP15_ARATH

Protein

Ubiquitin carboxyl-terminal hydrolase 15

Gene

UBP15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 2 (15 Jan 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei447 – 4471NucleophilePROSITE-ProRule annotation
    Active sitei703 – 7031Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri130 – 16738MYND-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. ubiquitinyl hydrolase activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:AT1G17110-MONOMER.
    ARA:GQT-1818-MONOMER.

    Protein family/group databases

    MEROPSiC19.096.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 15 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 15
    Short name:
    AtUBP15
    Ubiquitin thioesterase 15
    Ubiquitin-specific-processing protease 15
    Gene namesi
    Name:UBP15
    Ordered Locus Names:At1g17110
    ORF Names:F20D23.20
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G17110.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 924924Ubiquitin carboxyl-terminal hydrolase 15PRO_0000313041Add
    BLAST

    Proteomic databases

    PaxDbiQ9FPS9.
    PRIDEiQ9FPS9.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9FPS9.

    Interactioni

    Protein-protein interaction databases

    BioGridi23521. 3 interactions.
    STRINGi3702.AT1G17110.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FPS9.
    SMRiQ9FPS9. Positions 439-741.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini438 – 744307USPAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi172 – 1776Poly-Ser

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 MYND-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri130 – 16738MYND-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Transmembrane, Transmembrane helix, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5533.
    HOGENOMiHOG000242869.
    InParanoidiQ9FPS9.
    KOiK11855.
    PhylomeDBiQ9FPS9.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR002893. Znf_MYND.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    PF01753. zf-MYND. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS50235. USP_3. 1 hit.
    PS01360. ZF_MYND_1. 1 hit.
    PS50865. ZF_MYND_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9FPS9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLEPRGADIP ILFLVLVVLP VVAYILLGKW SNISEKRVRA NLLAQMAAEE    50
    ALRAETVVNA DRGVRFESVA TENRAQRTRT KTVSAGGGAV RAEFDAGARE 100
    TVAEQRSDSV TATCGVTVVA PVNNNELHVC ARCFGPAKTR CSRCKSVRYC 150
    SGKCQIIHWR VAHKDECVPV ESCSSSSERV SFEKDSVLYD HGMDSTMYSN 200
    NTTQAAKGKT SKSSVDFASL GISQNDITPQ INTQGRKSVG KQHSSKANRE 250
    SCRRDSATVF DSSDEAASAG GDNKTSHIKH KSRGNSYAAE TNPRRHSVDS 300
    SAVQMNGQSF VSGMQESHKH ENNLGVRSSF GCPNTQYPSN GTRTATLPRT 350
    GINKSGEQSC TETSKKGQVA AVSKTVRSKD TGISEESNGI SSTMGIMKMM 400
    GLRNSTKHDD RYKNLKMLFP YEEFLKFFQC EVFDLSPRGL VNCGNSCYAN 450
    AVLQSLTCTK PLVAYLLRRS HSRSCSGKDW CLMCELEQHV MMLRESGGPL 500
    SASRILSHMR SINCQIGDGS QEDAHEFLRL LVASMQSICL ERLGGETKVD 550
    PRLQETTLVQ HMFGGRLRSK VKCLRCDHES ERYENIMDLT LEIYGWVESL 600
    QDALTQFTRP EDLDGENMYR CSRCAGYVRA RKELSIHEAP NILTIVLKRF 650
    QEGRYGKINK CISFPEMLDM IPFMTRTGDV PPLYMLYAVI VHLDTLNASF 700
    SGHYISYVKD LRGNWYRIDD SEIHPVPMTQ VMSEGAYMLF YMRSYPRPQR 750
    GEHNGKAPVH HSQPRNEMKE QRKPVNRFKP RADHKNTESS SSEWSLFTSS 800
    DEASFTTEST RDSFSTIDYT DVCHVVDSSS PFAIFNNVYH NVEPSPHNTV 850
    ACRMFSGTKP ETRYFVEQET NHNNTVVLDA TPSLYPIPAP YPPHDYYDQS 900
    MYVNYETNPE FNNGQDQDRT YSYW 924
    Length:924
    Mass (Da):103,677
    Last modified:January 15, 2008 - v2
    Checksum:i32BD93822243271C
    GO

    Sequence cautioni

    The sequence AAD50020.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti106 – 1061R → K in AAG42756. (PubMed:11115897)Curated
    Sequence conflicti390 – 3901I → V in AAG42756. (PubMed:11115897)Curated
    Sequence conflicti536 – 5361Q → P in AAG42756. (PubMed:11115897)Curated
    Sequence conflicti568 – 5681R → H in AAG42756. (PubMed:11115897)Curated
    Sequence conflicti599 – 6002SL → FF in AAG42756. (PubMed:11115897)Curated
    Sequence conflicti605 – 6051T → I in AAG42756. (PubMed:11115897)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302665 mRNA. Translation: AAG42756.1.
    AC007651 Genomic DNA. Translation: AAD50020.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE29542.1.
    PIRiH86306.
    RefSeqiNP_564014.1. NM_101571.2. [Q9FPS9-1]
    UniGeneiAt.48199.

    Genome annotation databases

    EnsemblPlantsiAT1G17110.1; AT1G17110.1; AT1G17110. [Q9FPS9-1]
    GeneIDi838281.
    KEGGiath:AT1G17110.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302665 mRNA. Translation: AAG42756.1 .
    AC007651 Genomic DNA. Translation: AAD50020.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE29542.1 .
    PIRi H86306.
    RefSeqi NP_564014.1. NM_101571.2. [Q9FPS9-1 ]
    UniGenei At.48199.

    3D structure databases

    ProteinModelPortali Q9FPS9.
    SMRi Q9FPS9. Positions 439-741.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 23521. 3 interactions.
    STRINGi 3702.AT1G17110.1-P.

    Protein family/group databases

    MEROPSi C19.096.

    Proteomic databases

    PaxDbi Q9FPS9.
    PRIDEi Q9FPS9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G17110.1 ; AT1G17110.1 ; AT1G17110 . [Q9FPS9-1 ]
    GeneIDi 838281.
    KEGGi ath:AT1G17110.

    Organism-specific databases

    TAIRi AT1G17110.

    Phylogenomic databases

    eggNOGi COG5533.
    HOGENOMi HOG000242869.
    InParanoidi Q9FPS9.
    KOi K11855.
    PhylomeDBi Q9FPS9.

    Enzyme and pathway databases

    BioCyci ARA:AT1G17110-MONOMER.
    ARA:GQT-1818-MONOMER.

    Gene expression databases

    Genevestigatori Q9FPS9.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR002893. Znf_MYND.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    PF01753. zf-MYND. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS50235. USP_3. 1 hit.
    PS01360. ZF_MYND_1. 1 hit.
    PS50865. ZF_MYND_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
      Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
      Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiUBP15_ARATH
    AccessioniPrimary (citable) accession number: Q9FPS9
    Secondary accession number(s): Q9SHG9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: January 15, 2008
    Last modified: October 1, 2014
    This is version 84 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3