Q9FPS9 (UBP15_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 15
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 15
Short name=AtUBP15
Ubiquitin thiolesterase 15
Ubiquitin-specific-processing protease 15

Gene names

Name:	UBP15
Ordered Locus Names:	At1g17110
ORF Names:	F20D23.20

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	924 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the peptidase C19 family. Contains 1 MYND-type zinc finger.
Sequence caution	The sequence AAD50020.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell proliferation Inferred from mutant phenotype. Source: TAIR flower development Inferred from mutant phenotype. Source: TAIR fruit development Inferred from mutant phenotype. Source: TAIR leaf development Inferred from mutant phenotype. Source: TAIR protein deubiquitination Inferred from direct assay. Source: TAIR root development Inferred from mutant phenotype. Source: TAIR ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from direct assay. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay. Source: TAIR
Molecular function	ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro ubiquitin-specific protease activity Inferred from direct assay. Source: TAIR zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9FPS9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 924	924	Ubiquitin carboxyl-terminal hydrolase 15		PRO_0000313041
Regions
	Transmembrane	7 – 27	21	Helical; Potential
	Zinc finger	130 – 167	38	MYND-type
	Compositional bias	172 – 177	6	Poly-Ser
Sites
	Active site	447	1	Nucleophile By similarity
	Active site	703	1	Proton acceptor By similarity
Experimental info
	Sequence conflict	106	1	R → K in AAG42756. Ref.1
	Sequence conflict	390	1	I → V in AAG42756. Ref.1
	Sequence conflict	536	1	Q → P in AAG42756. Ref.1
	Sequence conflict	568	1	R → H in AAG42756. Ref.1
	Sequence conflict	599 – 600	2	SL → FF in AAG42756. Ref.1
	Sequence conflict	605	1	T → I in AAG42756. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 15, 2008. Version 2. Checksum: 32BD93822243271C	FASTA	924	103,677
	10 20 30 40 50 60 MLEPRGADIP ILFLVLVVLP VVAYILLGKW SNISEKRVRA NLLAQMAAEE ALRAETVVNA 70 80 90 100 110 120 DRGVRFESVA TENRAQRTRT KTVSAGGGAV RAEFDAGARE TVAEQRSDSV TATCGVTVVA 130 140 150 160 170 180 PVNNNELHVC ARCFGPAKTR CSRCKSVRYC SGKCQIIHWR VAHKDECVPV ESCSSSSERV 190 200 210 220 230 240 SFEKDSVLYD HGMDSTMYSN NTTQAAKGKT SKSSVDFASL GISQNDITPQ INTQGRKSVG 250 260 270 280 290 300 KQHSSKANRE SCRRDSATVF DSSDEAASAG GDNKTSHIKH KSRGNSYAAE TNPRRHSVDS 310 320 330 340 350 360 SAVQMNGQSF VSGMQESHKH ENNLGVRSSF GCPNTQYPSN GTRTATLPRT GINKSGEQSC 370 380 390 400 410 420 TETSKKGQVA AVSKTVRSKD TGISEESNGI SSTMGIMKMM GLRNSTKHDD RYKNLKMLFP 430 440 450 460 470 480 YEEFLKFFQC EVFDLSPRGL VNCGNSCYAN AVLQSLTCTK PLVAYLLRRS HSRSCSGKDW 490 500 510 520 530 540 CLMCELEQHV MMLRESGGPL SASRILSHMR SINCQIGDGS QEDAHEFLRL LVASMQSICL 550 560 570 580 590 600 ERLGGETKVD PRLQETTLVQ HMFGGRLRSK VKCLRCDHES ERYENIMDLT LEIYGWVESL 610 620 630 640 650 660 QDALTQFTRP EDLDGENMYR CSRCAGYVRA RKELSIHEAP NILTIVLKRF QEGRYGKINK 670 680 690 700 710 720 CISFPEMLDM IPFMTRTGDV PPLYMLYAVI VHLDTLNASF SGHYISYVKD LRGNWYRIDD 730 740 750 760 770 780 SEIHPVPMTQ VMSEGAYMLF YMRSYPRPQR GEHNGKAPVH HSQPRNEMKE QRKPVNRFKP 790 800 810 820 830 840 RADHKNTESS SSEWSLFTSS DEASFTTEST RDSFSTIDYT DVCHVVDSSS PFAIFNNVYH 850 860 870 880 890 900 NVEPSPHNTV ACRMFSGTKP ETRYFVEQET NHNNTVVLDA TPSLYPIPAP YPPHDYYDQS 910 920 MYVNYETNPE FNNGQDQDRT YSYW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine." Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D. Plant Physiol. 124:1828-1843(2000) [PubMed: 11115897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF302665 mRNA. Translation: AAG42756.1. AC007651 Genomic DNA. Translation: AAD50020.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE29542.1.
IPI	IPI00524333.
PIR	H86306.
RefSeq	NP_564014.1. NM_101571.2.
UniGene	At.48199.
3D structure databases
HSSP	HSSP built from PDB template 2DAN based on UniProtKB O75800.
ProteinModelPortal	Q9FPS9.
SMR	Q9FPS9. Positions 126-172, 438-744.
ModBase	Search...
Protein family/group databases
MEROPS	C19.096.
Proteomic databases
PRIDE	Q9FPS9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G17110.1; AT1G17110.1; AT1G17110.
GeneID	838281.
GenomeReviews	Gene locus AT1G17110 in contig CT485782_GR.
KEGG	ath:AT1G17110.
Organism-specific databases
TAIR	At1g17110.
Phylogenomic databases
eggNOG	KOG1865.
GeneTree	EPGT00070000028444.
HOGENOM	HBG319780.
InParanoid	Q9FPS9.
OMA	VMSEGAY.
PhylomeDB	Q9FPS9.
ProtClustDB	CLSN2917013.
Gene expression databases
Genevestigator	Q9FPS9.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR002893. Znf_MYND. [Graphical view]
Pfam	PF00443. UCH. 1 hit. PF01753. zf-MYND. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. False negative. PS50235. UCH_2_3. 1 hit. PS01360. ZF_MYND_1. 1 hit. PS50865. ZF_MYND_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP15_ARATH

Accession

Primary (citable) accession number: Q9FPS9
Secondary accession number(s): Q9SHG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 15, 2008
Last modified:	December 14, 2011
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program