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Q9FPS9

- UBP15_ARATH

UniProt

Q9FPS9 - UBP15_ARATH

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Protein

Ubiquitin carboxyl-terminal hydrolase 15

Gene

UBP15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei447 – 4471NucleophilePROSITE-ProRule annotation
Active sitei703 – 7031Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri130 – 16738MYND-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. ubiquitinyl hydrolase activity Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT1G17110-MONOMER.
ARA:GQT-1818-MONOMER.

Protein family/group databases

MEROPSiC19.096.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 15 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 15
Short name:
AtUBP15
Ubiquitin thioesterase 15
Ubiquitin-specific-processing protease 15
Gene namesi
Name:UBP15
Ordered Locus Names:At1g17110
ORF Names:F20D23.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G17110.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 924924Ubiquitin carboxyl-terminal hydrolase 15PRO_0000313041Add
BLAST

Proteomic databases

PaxDbiQ9FPS9.
PRIDEiQ9FPS9.

Expressioni

Gene expression databases

ExpressionAtlasiQ9FPS9. baseline and differential.
GenevestigatoriQ9FPS9.

Interactioni

Protein-protein interaction databases

BioGridi23521. 3 interactions.
STRINGi3702.AT1G17110.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FPS9.
SMRiQ9FPS9. Positions 439-741.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2721HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini438 – 744307USPAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi172 – 1776Poly-Ser

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 MYND-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri130 – 16738MYND-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiCOG5533.
HOGENOMiHOG000242869.
InParanoidiQ9FPS9.
KOiK11855.
PhylomeDBiQ9FPS9.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR002893. Znf_MYND.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF01753. zf-MYND. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS50235. USP_3. 1 hit.
PS01360. ZF_MYND_1. 1 hit.
PS50865. ZF_MYND_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9FPS9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLEPRGADIP ILFLVLVVLP VVAYILLGKW SNISEKRVRA NLLAQMAAEE
60 70 80 90 100
ALRAETVVNA DRGVRFESVA TENRAQRTRT KTVSAGGGAV RAEFDAGARE
110 120 130 140 150
TVAEQRSDSV TATCGVTVVA PVNNNELHVC ARCFGPAKTR CSRCKSVRYC
160 170 180 190 200
SGKCQIIHWR VAHKDECVPV ESCSSSSERV SFEKDSVLYD HGMDSTMYSN
210 220 230 240 250
NTTQAAKGKT SKSSVDFASL GISQNDITPQ INTQGRKSVG KQHSSKANRE
260 270 280 290 300
SCRRDSATVF DSSDEAASAG GDNKTSHIKH KSRGNSYAAE TNPRRHSVDS
310 320 330 340 350
SAVQMNGQSF VSGMQESHKH ENNLGVRSSF GCPNTQYPSN GTRTATLPRT
360 370 380 390 400
GINKSGEQSC TETSKKGQVA AVSKTVRSKD TGISEESNGI SSTMGIMKMM
410 420 430 440 450
GLRNSTKHDD RYKNLKMLFP YEEFLKFFQC EVFDLSPRGL VNCGNSCYAN
460 470 480 490 500
AVLQSLTCTK PLVAYLLRRS HSRSCSGKDW CLMCELEQHV MMLRESGGPL
510 520 530 540 550
SASRILSHMR SINCQIGDGS QEDAHEFLRL LVASMQSICL ERLGGETKVD
560 570 580 590 600
PRLQETTLVQ HMFGGRLRSK VKCLRCDHES ERYENIMDLT LEIYGWVESL
610 620 630 640 650
QDALTQFTRP EDLDGENMYR CSRCAGYVRA RKELSIHEAP NILTIVLKRF
660 670 680 690 700
QEGRYGKINK CISFPEMLDM IPFMTRTGDV PPLYMLYAVI VHLDTLNASF
710 720 730 740 750
SGHYISYVKD LRGNWYRIDD SEIHPVPMTQ VMSEGAYMLF YMRSYPRPQR
760 770 780 790 800
GEHNGKAPVH HSQPRNEMKE QRKPVNRFKP RADHKNTESS SSEWSLFTSS
810 820 830 840 850
DEASFTTEST RDSFSTIDYT DVCHVVDSSS PFAIFNNVYH NVEPSPHNTV
860 870 880 890 900
ACRMFSGTKP ETRYFVEQET NHNNTVVLDA TPSLYPIPAP YPPHDYYDQS
910 920
MYVNYETNPE FNNGQDQDRT YSYW
Length:924
Mass (Da):103,677
Last modified:January 15, 2008 - v2
Checksum:i32BD93822243271C
GO

Sequence cautioni

The sequence AAD50020.1 differs from that shown. Reason: Erroneous gene model prediction.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti106 – 1061R → K in AAG42756. (PubMed:11115897)Curated
Sequence conflicti390 – 3901I → V in AAG42756. (PubMed:11115897)Curated
Sequence conflicti536 – 5361Q → P in AAG42756. (PubMed:11115897)Curated
Sequence conflicti568 – 5681R → H in AAG42756. (PubMed:11115897)Curated
Sequence conflicti599 – 6002SL → FF in AAG42756. (PubMed:11115897)Curated
Sequence conflicti605 – 6051T → I in AAG42756. (PubMed:11115897)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF302665 mRNA. Translation: AAG42756.1.
AC007651 Genomic DNA. Translation: AAD50020.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29542.1.
PIRiH86306.
RefSeqiNP_564014.1. NM_101571.2. [Q9FPS9-1]
UniGeneiAt.48199.

Genome annotation databases

EnsemblPlantsiAT1G17110.1; AT1G17110.1; AT1G17110. [Q9FPS9-1]
GeneIDi838281.
KEGGiath:AT1G17110.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF302665 mRNA. Translation: AAG42756.1 .
AC007651 Genomic DNA. Translation: AAD50020.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE29542.1 .
PIRi H86306.
RefSeqi NP_564014.1. NM_101571.2. [Q9FPS9-1 ]
UniGenei At.48199.

3D structure databases

ProteinModelPortali Q9FPS9.
SMRi Q9FPS9. Positions 439-741.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 23521. 3 interactions.
STRINGi 3702.AT1G17110.1-P.

Protein family/group databases

MEROPSi C19.096.

Proteomic databases

PaxDbi Q9FPS9.
PRIDEi Q9FPS9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G17110.1 ; AT1G17110.1 ; AT1G17110 . [Q9FPS9-1 ]
GeneIDi 838281.
KEGGi ath:AT1G17110.

Organism-specific databases

TAIRi AT1G17110.

Phylogenomic databases

eggNOGi COG5533.
HOGENOMi HOG000242869.
InParanoidi Q9FPS9.
KOi K11855.
PhylomeDBi Q9FPS9.

Enzyme and pathway databases

BioCyci ARA:AT1G17110-MONOMER.
ARA:GQT-1818-MONOMER.

Gene expression databases

ExpressionAtlasi Q9FPS9. baseline and differential.
Genevestigatori Q9FPS9.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR002893. Znf_MYND.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
PF01753. zf-MYND. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS50235. USP_3. 1 hit.
PS01360. ZF_MYND_1. 1 hit.
PS50865. ZF_MYND_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
    Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
    Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiUBP15_ARATH
AccessioniPrimary (citable) accession number: Q9FPS9
Secondary accession number(s): Q9SHG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: October 29, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3