Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin carboxyl-terminal hydrolase 20

Gene

UBP20

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei185NucleophilePROSITE-ProRule annotation1
Active sitei435Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciARA:AT4G17895-MONOMER.

Protein family/group databases

MEROPSiC19.A16.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 20 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 20
Short name:
AtUBP20
Ubiquitin thioesterase 20
Ubiquitin-specific-processing protease 20
Gene namesi
Name:UBP20
Ordered Locus Names:At4g17895
ORF Names:T6K21.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G17895.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003130461 – 695Ubiquitin carboxyl-terminal hydrolase 20Add BLAST695

Proteomic databases

PaxDbiQ9FPS7.
PRIDEiQ9FPS7.

Expressioni

Gene expression databases

GenevisibleiQ9FPS7. AT.

Interactioni

Protein-protein interaction databases

BioGridi12806. 1 interactor.
STRINGi3702.AT4G17895.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FPS7.
SMRiQ9FPS7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini176 – 476USPAdd BLAST301

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi51 – 150Asp-richAdd BLAST100

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiKOG0706. Eukaryota.
KOG1865. Eukaryota.
ENOG410XQ92. LUCA.
HOGENOMiHOG000154757.
InParanoidiQ9FPS7.
KOiK11855.
OMAiWNLGNSC.
OrthoDBiEOG093604UC.
PhylomeDBiQ9FPS7.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FPS7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLMAKPDVPS SILPRSSSIL PNSIETLDEN ESIEAQVNNV QSLALSSPNR
60 70 80 90 100
DRSDDDDNNN NHDSVSIPPP IYDGYSSSSS DESQSVPSPP INLDHDDDEC
110 120 130 140 150
QIPIRNTSQA LDDIDDDIWG DDDLPETRRP WTPNVSPGFG SDDDDDNDDD
160 170 180 190 200
NSKNEPRKSL FYGFRQEPEP VTGVGAGLWN LGNSCFLNSV FQCFTHTVPL
210 220 230 240 250
IESLLSFRYE VPCHCGNEFF CVIRAIRYHI EAALRPERCP IAPYFFFDNL
260 270 280 290 300
NYFSPDFQRY QQEDAHEFLQ AFLEKLEICG SDRTSFRGDI TSQDVFSGRL
310 320 330 340 350
ISGLRCCNCD YVSETYEKSV GLSLEIEDVD TLGSALESFT RVEKLDEQLT
360 370 380 390 400
CDNCNEKVSK EKQLLLDKLP LVATFHLKRF KNNGLYMEKI YKHVKIPLEI
410 420 430 440 450
DLQPYMRNIQ ENEVSTKYHL YALVEHFGYS VAYGHYSSYV RSAPKIWHHF
460 470 480 490 500
DDSKVTRIDE DMVLSQDSYI LFYAREGTRW FSSVYEEMQP LVEASLLNSS
510 520 530 540 550
PKSVLDSSTN GECLSEISYE NGDKASKPCD SAGVCNQHVK TKEDFVSLSN
560 570 580 590 600
DDVFLSAESS SGEESPMGEL LDPLDPDDSY SPCTEKESDS CLAIERATIR
610 620 630 640 650
DDFFPLLLDQ NQESSTSSPK LQERTFEMQL LQMEETTKSQ EPWKQPLSSI
660 670 680 690
SNIADSMEAE FVYGDLMKKP SPRARELLDQ AISTNGSPPK KLKTT
Length:695
Mass (Da):78,633
Last modified:March 1, 2001 - v1
Checksum:i8FD6265E169892D2
GO

Sequence cautioni

The sequence CAA17132 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At4g17890 has been split into 2 genes: At4g17890 and At4g17895.Curated
The sequence CAB78791 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At4g17890 has been split into 2 genes: At4g17890 and At4g17895.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302668 mRNA. Translation: AAG42758.1.
AL021889 Genomic DNA. Translation: CAA17132.1. Sequence problems.
AL161547 Genomic DNA. Translation: CAB78791.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE83963.1.
PIRiT05075.
RefSeqiNP_567544.1. NM_117899.3.
UniGeneiAt.21461.

Genome annotation databases

EnsemblPlantsiAT4G17895.1; AT4G17895.1; AT4G17895.
GeneIDi827513.
GrameneiAT4G17895.1; AT4G17895.1; AT4G17895.
KEGGiath:AT4G17895.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302668 mRNA. Translation: AAG42758.1.
AL021889 Genomic DNA. Translation: CAA17132.1. Sequence problems.
AL161547 Genomic DNA. Translation: CAB78791.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE83963.1.
PIRiT05075.
RefSeqiNP_567544.1. NM_117899.3.
UniGeneiAt.21461.

3D structure databases

ProteinModelPortaliQ9FPS7.
SMRiQ9FPS7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi12806. 1 interactor.
STRINGi3702.AT4G17895.1.

Protein family/group databases

MEROPSiC19.A16.

Proteomic databases

PaxDbiQ9FPS7.
PRIDEiQ9FPS7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G17895.1; AT4G17895.1; AT4G17895.
GeneIDi827513.
GrameneiAT4G17895.1; AT4G17895.1; AT4G17895.
KEGGiath:AT4G17895.

Organism-specific databases

TAIRiAT4G17895.

Phylogenomic databases

eggNOGiKOG0706. Eukaryota.
KOG1865. Eukaryota.
ENOG410XQ92. LUCA.
HOGENOMiHOG000154757.
InParanoidiQ9FPS7.
KOiK11855.
OMAiWNLGNSC.
OrthoDBiEOG093604UC.
PhylomeDBiQ9FPS7.

Enzyme and pathway databases

BioCyciARA:AT4G17895-MONOMER.

Miscellaneous databases

PROiQ9FPS7.

Gene expression databases

GenevisibleiQ9FPS7. AT.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP20_ARATH
AccessioniPrimary (citable) accession number: Q9FPS7
Secondary accession number(s): O49688
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.