Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9FPS7

- UBP20_ARATH

UniProt

Q9FPS7 - UBP20_ARATH

Protein

Ubiquitin carboxyl-terminal hydrolase 20

Gene

UBP20

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei185 – 1851NucleophilePROSITE-ProRule annotation
    Active sitei435 – 4351Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitinyl hydrolase activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciARA:AT4G17895-MONOMER.

    Protein family/group databases

    MEROPSiC19.A16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 20 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 20
    Short name:
    AtUBP20
    Ubiquitin thioesterase 20
    Ubiquitin-specific-processing protease 20
    Gene namesi
    Name:UBP20
    Ordered Locus Names:At4g17895
    ORF Names:T6K21.70
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G17895.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 695695Ubiquitin carboxyl-terminal hydrolase 20PRO_0000313046Add
    BLAST

    Proteomic databases

    PRIDEiQ9FPS7.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9FPS7.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FPS7.
    SMRiQ9FPS7. Positions 177-473.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini176 – 476301USPAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi51 – 150100Asp-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5533.
    HOGENOMiHOG000154757.
    InParanoidiQ9FPS7.
    KOiK11855.
    OMAiIERATIR.
    PhylomeDBiQ9FPS7.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9FPS7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLMAKPDVPS SILPRSSSIL PNSIETLDEN ESIEAQVNNV QSLALSSPNR    50
    DRSDDDDNNN NHDSVSIPPP IYDGYSSSSS DESQSVPSPP INLDHDDDEC 100
    QIPIRNTSQA LDDIDDDIWG DDDLPETRRP WTPNVSPGFG SDDDDDNDDD 150
    NSKNEPRKSL FYGFRQEPEP VTGVGAGLWN LGNSCFLNSV FQCFTHTVPL 200
    IESLLSFRYE VPCHCGNEFF CVIRAIRYHI EAALRPERCP IAPYFFFDNL 250
    NYFSPDFQRY QQEDAHEFLQ AFLEKLEICG SDRTSFRGDI TSQDVFSGRL 300
    ISGLRCCNCD YVSETYEKSV GLSLEIEDVD TLGSALESFT RVEKLDEQLT 350
    CDNCNEKVSK EKQLLLDKLP LVATFHLKRF KNNGLYMEKI YKHVKIPLEI 400
    DLQPYMRNIQ ENEVSTKYHL YALVEHFGYS VAYGHYSSYV RSAPKIWHHF 450
    DDSKVTRIDE DMVLSQDSYI LFYAREGTRW FSSVYEEMQP LVEASLLNSS 500
    PKSVLDSSTN GECLSEISYE NGDKASKPCD SAGVCNQHVK TKEDFVSLSN 550
    DDVFLSAESS SGEESPMGEL LDPLDPDDSY SPCTEKESDS CLAIERATIR 600
    DDFFPLLLDQ NQESSTSSPK LQERTFEMQL LQMEETTKSQ EPWKQPLSSI 650
    SNIADSMEAE FVYGDLMKKP SPRARELLDQ AISTNGSPPK KLKTT 695
    Length:695
    Mass (Da):78,633
    Last modified:March 1, 2001 - v1
    Checksum:i8FD6265E169892D2
    GO

    Sequence cautioni

    The sequence CAA17132.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At4g17890 has been split into 2 genes: At4g17890 and At4g17895.
    The sequence CAB78791.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At4g17890 has been split into 2 genes: At4g17890 and At4g17895.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302668 mRNA. Translation: AAG42758.1.
    AL021889 Genomic DNA. Translation: CAA17132.1. Sequence problems.
    AL161547 Genomic DNA. Translation: CAB78791.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE83963.1.
    PIRiT05075.
    RefSeqiNP_567544.1. NM_117899.2.
    UniGeneiAt.21461.

    Genome annotation databases

    EnsemblPlantsiAT4G17895.1; AT4G17895.1; AT4G17895.
    GeneIDi827513.
    KEGGiath:AT4G17895.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302668 mRNA. Translation: AAG42758.1 .
    AL021889 Genomic DNA. Translation: CAA17132.1 . Sequence problems.
    AL161547 Genomic DNA. Translation: CAB78791.1 . Sequence problems.
    CP002687 Genomic DNA. Translation: AEE83963.1 .
    PIRi T05075.
    RefSeqi NP_567544.1. NM_117899.2.
    UniGenei At.21461.

    3D structure databases

    ProteinModelPortali Q9FPS7.
    SMRi Q9FPS7. Positions 177-473.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C19.A16.

    Proteomic databases

    PRIDEi Q9FPS7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G17895.1 ; AT4G17895.1 ; AT4G17895 .
    GeneIDi 827513.
    KEGGi ath:AT4G17895.

    Organism-specific databases

    GeneFarmi 1993. 220.
    TAIRi AT4G17895.

    Phylogenomic databases

    eggNOGi COG5533.
    HOGENOMi HOG000154757.
    InParanoidi Q9FPS7.
    KOi K11855.
    OMAi IERATIR.
    PhylomeDBi Q9FPS7.

    Enzyme and pathway databases

    BioCyci ARA:AT4G17895-MONOMER.

    Gene expression databases

    Genevestigatori Q9FPS7.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
      Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
      Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiUBP20_ARATH
    AccessioniPrimary (citable) accession number: Q9FPS7
    Secondary accession number(s): O49688
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3