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Q9FPS4

- UBP23_ARATH

UniProt

Q9FPS4 - UBP23_ARATH

Protein

Ubiquitin carboxyl-terminal hydrolase 23

Gene

UBP23

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 2 (15 Jan 2008)
      Previous versions | rss
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    Functioni

    Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei116 – 1161NucleophilePROSITE-ProRule annotation
    Active sitei369 – 3691Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitinyl hydrolase activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciARA:AT5G57990-MONOMER.

    Protein family/group databases

    MEROPSiC19.A12.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 23 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 23
    Short name:
    AtUBP23
    Ubiquitin thioesterase 23
    Ubiquitin-specific-processing protease 23
    Gene namesi
    Name:UBP23
    Ordered Locus Names:At5g57990
    ORF Names:MTI20.25
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G57990.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 859859Ubiquitin carboxyl-terminal hydrolase 23PRO_0000313049Add
    BLAST

    Proteomic databases

    PaxDbiQ9FPS4.
    PRIDEiQ9FPS4.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9FPS4.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FPS4.
    SMRiQ9FPS4. Positions 108-407.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini107 – 410304USPAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi814 – 8174Poly-Lys

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5077.
    HOGENOMiHOG000078724.
    InParanoidiQ9FPS4.
    KOiK11855.
    OMAiAHEYMIN.
    PhylomeDBiQ9FPS4.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9FPS4-1 [UniParc]FASTAAdd to Basket

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    MEVATSSTEI TIQTDRDPSS NNNGSCAVAS STASAVFRKI EFHPARKPFN    50
    GFSNGRSDFK IETLNPCSSN QRLLSAPSAK KPDSSDLLEH GFEPDLTFSI 100
    TFRKIGAGLQ NLGNTCFLNS VLQCLTYTEP LAATLQTAAH QKYCHVAGFC 150
    ALCAIQKHVR TARQANGRIL APKDLVSNLR CISRNFRNCR QEDAHEYMIN 200
    LLECMHKCSL PSGVPSESSD AYRRSLVHKI FGGSLRSQVK CEQCSHCSNK 250
    FDPFLDLSLD ISKADSLQRA LSRFTAVELL DNGAKVYQCE RCKQKVKAKK 300
    QLTVSKAPYV LTVHLKRFEA HRSEKIDRKV DFTSAIDMKP FVSGPHEGNL 350
    KYTLYGVLVH YGRSSHSGHY ACFVRTSSGM WYSLDDNRVV QVSEKTVFNQ 400
    KAYMLFYVRD RQNAVPKNSV PVVKKESFAT NRASLIVASN IKDQVNGSTV 450
    IKECGFGALV ANGLAPLKSC GPSTPAVLTQ KDLNAKETQN NAISNVEAKE 500
    ILETENGSAP VKTCDLAAPT VLVQKDLNTK EIFQKEVPLP QANGEGSLVK 550
    EDSKAACLIL PEKVSPHLDG SANAQTLVKL PTLGPKAENS VEEKNSLNNL 600
    NEPANSLKVI NVSVGNPPVE KAVLIDQTMG HHLEESATSI ESLKLTSERE 650
    TLTTPKKTRK PKTKTLKVEF KFFKLALGLR KKKVQRRERL STTVAGEIIS 700
    EELLSKKRVK YQDTSLIAPS KMISSSDGAV TSDQQQPVGS SDLSEASQNA 750
    KRKRESVLLQ KEAVNILTRG VPETVVAKWD EEISASQKRG SKSEGASSIG 800
    YVADEWDEEY DRGKKKKIRI KEESYRGPNP FQMLASKRQK ETKKKWTQSI 850
    TDAKTAYRI 859
    Length:859
    Mass (Da):94,903
    Last modified:January 15, 2008 - v2
    Checksum:i046FE8764DBB4609
    GO

    Sequence cautioni

    The sequence BAB08869.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti657 – 6571K → N in AAG42761. (PubMed:11115897)Curated
    Sequence conflicti660 – 6601K → E in AAG42761. (PubMed:11115897)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302671 mRNA. Translation: AAG42761.1.
    AB013396 Genomic DNA. Translation: BAB08869.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED96982.1.
    RefSeqiNP_568873.1. NM_125184.2.
    UniGeneiAt.22531.

    Genome annotation databases

    EnsemblPlantsiAT5G57990.1; AT5G57990.1; AT5G57990.
    GeneIDi835910.
    KEGGiath:AT5G57990.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302671 mRNA. Translation: AAG42761.1 .
    AB013396 Genomic DNA. Translation: BAB08869.1 . Sequence problems.
    CP002688 Genomic DNA. Translation: AED96982.1 .
    RefSeqi NP_568873.1. NM_125184.2.
    UniGenei At.22531.

    3D structure databases

    ProteinModelPortali Q9FPS4.
    SMRi Q9FPS4. Positions 108-407.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C19.A12.

    Proteomic databases

    PaxDbi Q9FPS4.
    PRIDEi Q9FPS4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G57990.1 ; AT5G57990.1 ; AT5G57990 .
    GeneIDi 835910.
    KEGGi ath:AT5G57990.

    Organism-specific databases

    GeneFarmi 1994. 220.
    TAIRi AT5G57990.

    Phylogenomic databases

    eggNOGi COG5077.
    HOGENOMi HOG000078724.
    InParanoidi Q9FPS4.
    KOi K11855.
    OMAi AHEYMIN.
    PhylomeDBi Q9FPS4.

    Enzyme and pathway databases

    BioCyci ARA:AT5G57990-MONOMER.

    Gene expression databases

    Genevestigatori Q9FPS4.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
      Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
      Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
      Strain: cv. Columbia.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
      Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
      DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiUBP23_ARATH
    AccessioniPrimary (citable) accession number: Q9FPS4
    Secondary accession number(s): Q9FJL7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: January 15, 2008
    Last modified: October 1, 2014
    This is version 71 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3