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Q9FPS4 (UBP23_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 23

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 23
Short name=AtUBP23
Ubiquitin thioesterase 23
Ubiquitin-specific-processing protease 23
Gene names
Name:UBP23
Ordered Locus Names:At5g57990
ORF Names:MTI20.25
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length859 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 USP domain.

Sequence caution

The sequence BAB08869.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncysteine-type peptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 859859Ubiquitin carboxyl-terminal hydrolase 23
PRO_0000313049

Regions

Domain107 – 410304USP
Compositional bias814 – 8174Poly-Lys

Sites

Active site1161Nucleophile By similarity
Active site3691Proton acceptor By similarity

Experimental info

Sequence conflict6571K → N in AAG42761. Ref.1
Sequence conflict6601K → E in AAG42761. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9FPS4 [UniParc].

Last modified January 15, 2008. Version 2.
Checksum: 046FE8764DBB4609

FASTA85994,903
        10         20         30         40         50         60 
MEVATSSTEI TIQTDRDPSS NNNGSCAVAS STASAVFRKI EFHPARKPFN GFSNGRSDFK 

        70         80         90        100        110        120 
IETLNPCSSN QRLLSAPSAK KPDSSDLLEH GFEPDLTFSI TFRKIGAGLQ NLGNTCFLNS 

       130        140        150        160        170        180 
VLQCLTYTEP LAATLQTAAH QKYCHVAGFC ALCAIQKHVR TARQANGRIL APKDLVSNLR 

       190        200        210        220        230        240 
CISRNFRNCR QEDAHEYMIN LLECMHKCSL PSGVPSESSD AYRRSLVHKI FGGSLRSQVK 

       250        260        270        280        290        300 
CEQCSHCSNK FDPFLDLSLD ISKADSLQRA LSRFTAVELL DNGAKVYQCE RCKQKVKAKK 

       310        320        330        340        350        360 
QLTVSKAPYV LTVHLKRFEA HRSEKIDRKV DFTSAIDMKP FVSGPHEGNL KYTLYGVLVH 

       370        380        390        400        410        420 
YGRSSHSGHY ACFVRTSSGM WYSLDDNRVV QVSEKTVFNQ KAYMLFYVRD RQNAVPKNSV 

       430        440        450        460        470        480 
PVVKKESFAT NRASLIVASN IKDQVNGSTV IKECGFGALV ANGLAPLKSC GPSTPAVLTQ 

       490        500        510        520        530        540 
KDLNAKETQN NAISNVEAKE ILETENGSAP VKTCDLAAPT VLVQKDLNTK EIFQKEVPLP 

       550        560        570        580        590        600 
QANGEGSLVK EDSKAACLIL PEKVSPHLDG SANAQTLVKL PTLGPKAENS VEEKNSLNNL 

       610        620        630        640        650        660 
NEPANSLKVI NVSVGNPPVE KAVLIDQTMG HHLEESATSI ESLKLTSERE TLTTPKKTRK 

       670        680        690        700        710        720 
PKTKTLKVEF KFFKLALGLR KKKVQRRERL STTVAGEIIS EELLSKKRVK YQDTSLIAPS 

       730        740        750        760        770        780 
KMISSSDGAV TSDQQQPVGS SDLSEASQNA KRKRESVLLQ KEAVNILTRG VPETVVAKWD 

       790        800        810        820        830        840 
EEISASQKRG SKSEGASSIG YVADEWDEEY DRGKKKKIRI KEESYRGPNP FQMLASKRQK 

       850 
ETKKKWTQSI TDAKTAYRI 

« Hide

References

« Hide 'large scale' references
[1]"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF302671 mRNA. Translation: AAG42761.1.
AB013396 Genomic DNA. Translation: BAB08869.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED96982.1.
RefSeqNP_568873.1. NM_125184.2.
UniGeneAt.22531.

3D structure databases

ProteinModelPortalQ9FPS4.
SMRQ9FPS4. Positions 108-407.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC19.A12.

Proteomic databases

PaxDbQ9FPS4.
PRIDEQ9FPS4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G57990.1; AT5G57990.1; AT5G57990.
GeneID835910.
KEGGath:AT5G57990.

Organism-specific databases

GeneFarm1994. 220.
TAIRAT5G57990.

Phylogenomic databases

eggNOGCOG5077.
HOGENOMHOG000078724.
InParanoidQ9FPS4.
KOK11855.
OMAAHEYMIN.
PhylomeDBQ9FPS4.

Enzyme and pathway databases

BioCycARA:AT5G57990-MONOMER.

Gene expression databases

GenevestigatorQ9FPS4.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP23_ARATH
AccessionPrimary (citable) accession number: Q9FPS4
Secondary accession number(s): Q9FJL7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: May 14, 2014
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names