Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin carboxyl-terminal hydrolase 23

Gene

UBP23

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei116 – 1161NucleophilePROSITE-ProRule annotation
Active sitei369 – 3691Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciARA:AT5G57990-MONOMER.

Protein family/group databases

MEROPSiC19.A12.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 23 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 23
Short name:
AtUBP23
Ubiquitin thioesterase 23
Ubiquitin-specific-processing protease 23
Gene namesi
Name:UBP23
Ordered Locus Names:At5g57990
ORF Names:MTI20.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G57990.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 859859Ubiquitin carboxyl-terminal hydrolase 23PRO_0000313049Add
BLAST

Proteomic databases

PaxDbiQ9FPS4.
PRIDEiQ9FPS4.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G57990.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FPS4.
SMRiQ9FPS4. Positions 108-407.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini107 – 410304USPAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi814 – 8174Poly-Lys

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5077.
HOGENOMiHOG000078724.
InParanoidiQ9FPS4.
KOiK11855.
OMAiAHEYMIN.
PhylomeDBiQ9FPS4.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FPS4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVATSSTEI TIQTDRDPSS NNNGSCAVAS STASAVFRKI EFHPARKPFN
60 70 80 90 100
GFSNGRSDFK IETLNPCSSN QRLLSAPSAK KPDSSDLLEH GFEPDLTFSI
110 120 130 140 150
TFRKIGAGLQ NLGNTCFLNS VLQCLTYTEP LAATLQTAAH QKYCHVAGFC
160 170 180 190 200
ALCAIQKHVR TARQANGRIL APKDLVSNLR CISRNFRNCR QEDAHEYMIN
210 220 230 240 250
LLECMHKCSL PSGVPSESSD AYRRSLVHKI FGGSLRSQVK CEQCSHCSNK
260 270 280 290 300
FDPFLDLSLD ISKADSLQRA LSRFTAVELL DNGAKVYQCE RCKQKVKAKK
310 320 330 340 350
QLTVSKAPYV LTVHLKRFEA HRSEKIDRKV DFTSAIDMKP FVSGPHEGNL
360 370 380 390 400
KYTLYGVLVH YGRSSHSGHY ACFVRTSSGM WYSLDDNRVV QVSEKTVFNQ
410 420 430 440 450
KAYMLFYVRD RQNAVPKNSV PVVKKESFAT NRASLIVASN IKDQVNGSTV
460 470 480 490 500
IKECGFGALV ANGLAPLKSC GPSTPAVLTQ KDLNAKETQN NAISNVEAKE
510 520 530 540 550
ILETENGSAP VKTCDLAAPT VLVQKDLNTK EIFQKEVPLP QANGEGSLVK
560 570 580 590 600
EDSKAACLIL PEKVSPHLDG SANAQTLVKL PTLGPKAENS VEEKNSLNNL
610 620 630 640 650
NEPANSLKVI NVSVGNPPVE KAVLIDQTMG HHLEESATSI ESLKLTSERE
660 670 680 690 700
TLTTPKKTRK PKTKTLKVEF KFFKLALGLR KKKVQRRERL STTVAGEIIS
710 720 730 740 750
EELLSKKRVK YQDTSLIAPS KMISSSDGAV TSDQQQPVGS SDLSEASQNA
760 770 780 790 800
KRKRESVLLQ KEAVNILTRG VPETVVAKWD EEISASQKRG SKSEGASSIG
810 820 830 840 850
YVADEWDEEY DRGKKKKIRI KEESYRGPNP FQMLASKRQK ETKKKWTQSI

TDAKTAYRI
Length:859
Mass (Da):94,903
Last modified:January 15, 2008 - v2
Checksum:i046FE8764DBB4609
GO

Sequence cautioni

The sequence BAB08869.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti657 – 6571K → N in AAG42761 (PubMed:11115897).Curated
Sequence conflicti660 – 6601K → E in AAG42761 (PubMed:11115897).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302671 mRNA. Translation: AAG42761.1.
AB013396 Genomic DNA. Translation: BAB08869.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED96982.1.
RefSeqiNP_568873.1. NM_125184.2.
UniGeneiAt.22531.

Genome annotation databases

EnsemblPlantsiAT5G57990.1; AT5G57990.1; AT5G57990.
GeneIDi835910.
KEGGiath:AT5G57990.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302671 mRNA. Translation: AAG42761.1.
AB013396 Genomic DNA. Translation: BAB08869.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED96982.1.
RefSeqiNP_568873.1. NM_125184.2.
UniGeneiAt.22531.

3D structure databases

ProteinModelPortaliQ9FPS4.
SMRiQ9FPS4. Positions 108-407.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G57990.1.

Protein family/group databases

MEROPSiC19.A12.

Proteomic databases

PaxDbiQ9FPS4.
PRIDEiQ9FPS4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G57990.1; AT5G57990.1; AT5G57990.
GeneIDi835910.
KEGGiath:AT5G57990.

Organism-specific databases

GeneFarmi1994. 220.
TAIRiAT5G57990.

Phylogenomic databases

eggNOGiCOG5077.
HOGENOMiHOG000078724.
InParanoidiQ9FPS4.
KOiK11855.
OMAiAHEYMIN.
PhylomeDBiQ9FPS4.

Enzyme and pathway databases

BioCyciARA:AT5G57990-MONOMER.

Miscellaneous databases

PROiQ9FPS4.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
    Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
    Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
    Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
    DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiUBP23_ARATH
AccessioniPrimary (citable) accession number: Q9FPS4
Secondary accession number(s): Q9FJL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: June 24, 2015
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.