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Q9FPS2

- UBP25_ARATH

UniProt

Q9FPS2 - UBP25_ARATH

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Protein

Ubiquitin carboxyl-terminal hydrolase 25

Gene

UBP25

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei33 – 331NucleophilePROSITE-ProRule annotation
Active sitei294 – 2941Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. ubiquitinyl hydrolase activity Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciARA:AT3G14400-MONOMER.

Protein family/group databases

MEROPSiC19.A13.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 25 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 25
Short name:
AtUBP25
Ubiquitin thioesterase 25
Ubiquitin-specific-processing protease 25
Gene namesi
Name:UBP25
Ordered Locus Names:At3g14400
ORF Names:MLN21.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G14400.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 661661Ubiquitin carboxyl-terminal hydrolase 25PRO_0000313051Add
BLAST

Proteomic databases

PRIDEiQ9FPS2.

Expressioni

Gene expression databases

GenevestigatoriQ9FPS2.

Interactioni

Protein-protein interaction databases

IntActiQ9FPS2. 1 interaction.
STRINGi3702.AT3G14400.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FPS2.
SMRiQ9FPS2. Positions 15-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 335312USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5077.
HOGENOMiHOG000084095.
InParanoidiQ9FPS2.
KOiK11855.
OMAiKAYILFF.
PhylomeDBiQ9FPS2.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FPS2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGFKLQMSWM PSLLSQKRRN GPPLGLRNLG NTCYLNSVLQ CLTFTPPLAN
60 70 80 90 100
FCLTHKHSSH CDTYVDGERK RDCPFCIVEK RIARSLSVDL TTDAPNKISS
110 120 130 140 150
CLKIFAEHFK LGRQEDAHEF LRYVIDACHN TSLRLKKLRY NGNEPFNGNS
160 170 180 190 200
VVKEIFGGAL QSQVKCLSCG AESNKADEIM DISLEILQSS SVKESLQKFF
210 220 230 240 250
QSEILDGNNK YRCESCEKLV TARKQMSILQ APNILVIQLK RFGGIFGGKI
260 270 280 290 300
DKAISFGEIL VLSNFMSKAS KDPQPEYKLF GIIVHSGFSP ESGHYYAYVK
310 320 330 340 350
DSLGRWYCCN DSFVSLSTLQ EVLSEKAYIL FFSRSNQRPA SAKTLVTSNG
360 370 380 390 400
TTSHEVNGCE TSNPQKFIGP LNGFNMKPQA EQSFQKGNLA SSKPHKFIRP
410 420 430 440 450
KPRAEQAPLE DNLLSSKVEK APLRPHAKVS ISVNLGAKRV SPVNGRLSFH
460 470 480 490 500
QDENIAPKAN KENSVSVLPT KVNSGTERKF GTENGGNGVK ENGSAPGSSN
510 520 530 540 550
HKVALHPHER SNGSSNGGDH HKDNLHPCGS NGSQNGTAHP ETERNGVSTT
560 570 580 590 600
QSKGLCSSTK EDPCILLRKD ESSRNELEAI KESLKKDALS HLRSCGWYDK
610 620 630 640 650
VLISMHAKKR LRTEQSGGED GSDLKRRLIE DVKSSLKSQI PEELKADLVN
660
RIWEISKKKY S
Length:661
Mass (Da):73,293
Last modified:March 1, 2001 - v1
Checksum:iD47DEAD85E8D9B8E
GO

Sequence cautioni

The sequence BAB01045.1 differs from that shown. Reason: Erroneous gene model prediction.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti114 – 1141Q → E in AAL57643. (PubMed:14593172)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF302673 mRNA. Translation: AAG42763.1.
AB022220 Genomic DNA. Translation: BAB01045.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75513.1.
AY064992 mRNA. Translation: AAL57643.1.
BT021128 mRNA. Translation: AAX22263.1.
RefSeqiNP_566486.1. NM_112299.3.
UniGeneiAt.6074.

Genome annotation databases

EnsemblPlantsiAT3G14400.1; AT3G14400.1; AT3G14400.
GeneIDi820662.
KEGGiath:AT3G14400.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF302673 mRNA. Translation: AAG42763.1 .
AB022220 Genomic DNA. Translation: BAB01045.1 . Sequence problems.
CP002686 Genomic DNA. Translation: AEE75513.1 .
AY064992 mRNA. Translation: AAL57643.1 .
BT021128 mRNA. Translation: AAX22263.1 .
RefSeqi NP_566486.1. NM_112299.3.
UniGenei At.6074.

3D structure databases

ProteinModelPortali Q9FPS2.
SMRi Q9FPS2. Positions 15-333.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9FPS2. 1 interaction.
STRINGi 3702.AT3G14400.1-P.

Protein family/group databases

MEROPSi C19.A13.

Proteomic databases

PRIDEi Q9FPS2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G14400.1 ; AT3G14400.1 ; AT3G14400 .
GeneIDi 820662.
KEGGi ath:AT3G14400.

Organism-specific databases

TAIRi AT3G14400.

Phylogenomic databases

eggNOGi COG5077.
HOGENOMi HOG000084095.
InParanoidi Q9FPS2.
KOi K11855.
OMAi KAYILFF.
PhylomeDBi Q9FPS2.

Enzyme and pathway databases

BioCyci ARA:AT3G14400-MONOMER.

Gene expression databases

Genevestigatori Q9FPS2.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
    Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
    Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiUBP25_ARATH
AccessioniPrimary (citable) accession number: Q9FPS2
Secondary accession number(s): Q8VZF5, Q9LUK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2001
Last modified: October 1, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3