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Q9FPS2

- UBP25_ARATH

UniProt

Q9FPS2 - UBP25_ARATH

Protein

Ubiquitin carboxyl-terminal hydrolase 25

Gene

UBP25

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei33 – 331NucleophilePROSITE-ProRule annotation
    Active sitei294 – 2941Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitinyl hydrolase activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciARA:AT3G14400-MONOMER.

    Protein family/group databases

    MEROPSiC19.A13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 25 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 25
    Short name:
    AtUBP25
    Ubiquitin thioesterase 25
    Ubiquitin-specific-processing protease 25
    Gene namesi
    Name:UBP25
    Ordered Locus Names:At3g14400
    ORF Names:MLN21.18
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G14400.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 661661Ubiquitin carboxyl-terminal hydrolase 25PRO_0000313051Add
    BLAST

    Proteomic databases

    PRIDEiQ9FPS2.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9FPS2.

    Interactioni

    Protein-protein interaction databases

    IntActiQ9FPS2. 1 interaction.
    STRINGi3702.AT3G14400.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FPS2.
    SMRiQ9FPS2. Positions 15-333.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini24 – 335312USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5077.
    HOGENOMiHOG000084095.
    InParanoidiQ9FPS2.
    KOiK11855.
    OMAiKAYILFF.
    PhylomeDBiQ9FPS2.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9FPS2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGFKLQMSWM PSLLSQKRRN GPPLGLRNLG NTCYLNSVLQ CLTFTPPLAN    50
    FCLTHKHSSH CDTYVDGERK RDCPFCIVEK RIARSLSVDL TTDAPNKISS 100
    CLKIFAEHFK LGRQEDAHEF LRYVIDACHN TSLRLKKLRY NGNEPFNGNS 150
    VVKEIFGGAL QSQVKCLSCG AESNKADEIM DISLEILQSS SVKESLQKFF 200
    QSEILDGNNK YRCESCEKLV TARKQMSILQ APNILVIQLK RFGGIFGGKI 250
    DKAISFGEIL VLSNFMSKAS KDPQPEYKLF GIIVHSGFSP ESGHYYAYVK 300
    DSLGRWYCCN DSFVSLSTLQ EVLSEKAYIL FFSRSNQRPA SAKTLVTSNG 350
    TTSHEVNGCE TSNPQKFIGP LNGFNMKPQA EQSFQKGNLA SSKPHKFIRP 400
    KPRAEQAPLE DNLLSSKVEK APLRPHAKVS ISVNLGAKRV SPVNGRLSFH 450
    QDENIAPKAN KENSVSVLPT KVNSGTERKF GTENGGNGVK ENGSAPGSSN 500
    HKVALHPHER SNGSSNGGDH HKDNLHPCGS NGSQNGTAHP ETERNGVSTT 550
    QSKGLCSSTK EDPCILLRKD ESSRNELEAI KESLKKDALS HLRSCGWYDK 600
    VLISMHAKKR LRTEQSGGED GSDLKRRLIE DVKSSLKSQI PEELKADLVN 650
    RIWEISKKKY S 661
    Length:661
    Mass (Da):73,293
    Last modified:March 1, 2001 - v1
    Checksum:iD47DEAD85E8D9B8E
    GO

    Sequence cautioni

    The sequence BAB01045.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti114 – 1141Q → E in AAL57643. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302673 mRNA. Translation: AAG42763.1.
    AB022220 Genomic DNA. Translation: BAB01045.1. Sequence problems.
    CP002686 Genomic DNA. Translation: AEE75513.1.
    AY064992 mRNA. Translation: AAL57643.1.
    BT021128 mRNA. Translation: AAX22263.1.
    RefSeqiNP_566486.1. NM_112299.3.
    UniGeneiAt.6074.

    Genome annotation databases

    EnsemblPlantsiAT3G14400.1; AT3G14400.1; AT3G14400.
    GeneIDi820662.
    KEGGiath:AT3G14400.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302673 mRNA. Translation: AAG42763.1 .
    AB022220 Genomic DNA. Translation: BAB01045.1 . Sequence problems.
    CP002686 Genomic DNA. Translation: AEE75513.1 .
    AY064992 mRNA. Translation: AAL57643.1 .
    BT021128 mRNA. Translation: AAX22263.1 .
    RefSeqi NP_566486.1. NM_112299.3.
    UniGenei At.6074.

    3D structure databases

    ProteinModelPortali Q9FPS2.
    SMRi Q9FPS2. Positions 15-333.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9FPS2. 1 interaction.
    STRINGi 3702.AT3G14400.1-P.

    Protein family/group databases

    MEROPSi C19.A13.

    Proteomic databases

    PRIDEi Q9FPS2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G14400.1 ; AT3G14400.1 ; AT3G14400 .
    GeneIDi 820662.
    KEGGi ath:AT3G14400.

    Organism-specific databases

    TAIRi AT3G14400.

    Phylogenomic databases

    eggNOGi COG5077.
    HOGENOMi HOG000084095.
    InParanoidi Q9FPS2.
    KOi K11855.
    OMAi KAYILFF.
    PhylomeDBi Q9FPS2.

    Enzyme and pathway databases

    BioCyci ARA:AT3G14400-MONOMER.

    Gene expression databases

    Genevestigatori Q9FPS2.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
      Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
      Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
      Strain: cv. Columbia.
    2. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
      DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiUBP25_ARATH
    AccessioniPrimary (citable) accession number: Q9FPS2
    Secondary accession number(s): Q8VZF5, Q9LUK9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3