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Q9FPS2 (UBP25_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 25

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 25
Short name=AtUBP25
Ubiquitin thioesterase 25
Ubiquitin-specific-processing protease 25
Gene names
Name:UBP25
Ordered Locus Names:At3g14400
ORF Names:MLN21.18
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length661 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 USP domain.

Sequence caution

The sequence BAB01045.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncysteine-type peptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 661661Ubiquitin carboxyl-terminal hydrolase 25
PRO_0000313051

Regions

Domain24 – 335312USP

Sites

Active site331Nucleophile By similarity
Active site2941Proton acceptor By similarity

Experimental info

Sequence conflict1141Q → E in AAL57643. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9FPS2 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: D47DEAD85E8D9B8E

FASTA66173,293
        10         20         30         40         50         60 
MGFKLQMSWM PSLLSQKRRN GPPLGLRNLG NTCYLNSVLQ CLTFTPPLAN FCLTHKHSSH 

        70         80         90        100        110        120 
CDTYVDGERK RDCPFCIVEK RIARSLSVDL TTDAPNKISS CLKIFAEHFK LGRQEDAHEF 

       130        140        150        160        170        180 
LRYVIDACHN TSLRLKKLRY NGNEPFNGNS VVKEIFGGAL QSQVKCLSCG AESNKADEIM 

       190        200        210        220        230        240 
DISLEILQSS SVKESLQKFF QSEILDGNNK YRCESCEKLV TARKQMSILQ APNILVIQLK 

       250        260        270        280        290        300 
RFGGIFGGKI DKAISFGEIL VLSNFMSKAS KDPQPEYKLF GIIVHSGFSP ESGHYYAYVK 

       310        320        330        340        350        360 
DSLGRWYCCN DSFVSLSTLQ EVLSEKAYIL FFSRSNQRPA SAKTLVTSNG TTSHEVNGCE 

       370        380        390        400        410        420 
TSNPQKFIGP LNGFNMKPQA EQSFQKGNLA SSKPHKFIRP KPRAEQAPLE DNLLSSKVEK 

       430        440        450        460        470        480 
APLRPHAKVS ISVNLGAKRV SPVNGRLSFH QDENIAPKAN KENSVSVLPT KVNSGTERKF 

       490        500        510        520        530        540 
GTENGGNGVK ENGSAPGSSN HKVALHPHER SNGSSNGGDH HKDNLHPCGS NGSQNGTAHP 

       550        560        570        580        590        600 
ETERNGVSTT QSKGLCSSTK EDPCILLRKD ESSRNELEAI KESLKKDALS HLRSCGWYDK 

       610        620        630        640        650        660 
VLISMHAKKR LRTEQSGGED GSDLKRRLIE DVKSSLKSQI PEELKADLVN RIWEISKKKY 


S 

« Hide

References

« Hide 'large scale' references
[1]"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF302673 mRNA. Translation: AAG42763.1.
AB022220 Genomic DNA. Translation: BAB01045.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75513.1.
AY064992 mRNA. Translation: AAL57643.1.
BT021128 mRNA. Translation: AAX22263.1.
RefSeqNP_566486.1. NM_112299.3.
UniGeneAt.6074.

3D structure databases

ProteinModelPortalQ9FPS2.
SMRQ9FPS2. Positions 15-333.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9FPS2. 1 interaction.
STRING3702.AT3G14400.1-P.

Protein family/group databases

MEROPSC19.A13.

Proteomic databases

PRIDEQ9FPS2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G14400.1; AT3G14400.1; AT3G14400.
GeneID820662.
KEGGath:AT3G14400.

Organism-specific databases

TAIRAT3G14400.

Phylogenomic databases

eggNOGCOG5077.
HOGENOMHOG000084095.
InParanoidQ9FPS2.
KOK11855.
OMAKAYILFF.
PhylomeDBQ9FPS2.

Enzyme and pathway databases

BioCycARA:AT3G14400-MONOMER.

Gene expression databases

GenevestigatorQ9FPS2.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP25_ARATH
AccessionPrimary (citable) accession number: Q9FPS2
Secondary accession number(s): Q8VZF5, Q9LUK9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2001
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names