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Q9FPS0

- UBP27_ARATH

UniProt

Q9FPS0 - UBP27_ARATH

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Protein

Ubiquitin carboxyl-terminal hydrolase 27

Gene

UBP27

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei83 – 831NucleophilePROSITE-ProRule annotation
Active sitei440 – 4401Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. ubiquitinyl hydrolase activity Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciARA:AT4G39370-MONOMER.
ARA:GQT-496-MONOMER.
ARA:GQT-497-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 27 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 27
Short name:
AtUBP27
Ubiquitin thioesterase 27
Ubiquitin-specific-processing protease 27
Gene namesi
Name:UBP27
Ordered Locus Names:At4g39370
ORF Names:F23K16.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G39370.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei30 – 5021HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Ubiquitin carboxyl-terminal hydrolase 27PRO_0000313052Add
BLAST

Proteomic databases

PaxDbiQ9FPS0.
PRIDEiQ9FPS0.

Expressioni

Gene expression databases

ExpressionAtlasiQ9FPS0. baseline and differential.
GenevestigatoriQ9FPS0.

Interactioni

Protein-protein interaction databases

BioGridi15372. 1 interaction.
STRINGi3702.AT4G39370.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FPS0.
SMRiQ9FPS0. Positions 75-492.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini74 – 494421USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5533.
HOGENOMiHOG000070653.
InParanoidiQ9FPS0.
KOiK11851.
PhylomeDBiQ9FPS0.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9FPS0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVSRRGSETK AIVCVLTDRI RISNQWVSHL SFAGLLGVAG FVFAQQHGLF
60 70 80 90 100
RNLNNLKLFS GREKDSGDDS FLVPGLQNLG NNCFLNVILQ ALASCKDFRS
110 120 130 140 150
FLQWVLEDAR GSLAGEQEEQ LPLTFALSAL LQELGTVGSR RSVSNPRKVM
160 170 180 190 200
VTLTDYAKNF NLTSQQDAAE ALLHLISSLQ EEIVVCYRPS QSSNLSDILF
210 220 230 240 250
SRNLRMLAPS EGLHGLMELK RWHKHLRGPF DGILGSTLMC RTCSSQISLE
260 270 280 290 300
FQFFHTLPLS PLLHHGGYNI MSGCTLEHCL KKFLNTEKVE NYFCYRCWHG
310 320 330 340 350
AALKYLSVIG AAETEIEKLR SCGGEDQCDC KTSLHLQRMP WSNSYSHILK
360 370 380 390 400
QLIIARFPKL LCIQVQRASF NMFEEFKLSG HIAFPLVLNL SLFTPSSIGV
410 420 430 440 450
NIEERIEMSS EYQKPEASKN HGMYRLVTVV EHFGRTGSGH YTVYRSVRVF
460 470 480 490
SQEEEEEDCD EDLSWFSISD SEVCRVSESD VLGAEASLLF YERL
Length:494
Mass (Da):55,771
Last modified:March 1, 2001 - v1
Checksum:iB8CBDF5799FA9FA6
GO
Isoform 2 (identifier: Q9FPS0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     314-361: TEIEKLRSCG...LIIARFPKLL → VVNLSQLLML...AQELWRRGPM
     362-494: Missing.

Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.

Show »
Length:361
Mass (Da):40,770
Checksum:iEBD855CB345C1129
GO

Sequence cautioni

The sequence CAB52824.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB80600.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei314 – 36148TEIEK…FPKLL → VVNLSQLLMLLYFSFVHASR LYLTRVSWKRFQSDGNRKAQ ELWRRGPM in isoform 2. CuratedVSP_029992Add
BLAST
Alternative sequencei362 – 494133Missing in isoform 2. CuratedVSP_029993Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302675 mRNA. Translation: AAG42765.1.
AL078620 Genomic DNA. Translation: CAB52824.1. Sequence problems.
AL161595 Genomic DNA. Translation: CAB80600.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE87061.1.
CP002687 Genomic DNA. Translation: AEE87062.1.
AF370208 mRNA. Translation: AAK44023.1.
AY133770 mRNA. Translation: AAM91704.1.
PIRiB85466.
RefSeqiNP_568058.1. NM_120097.2. [Q9FPS0-1]
NP_974714.1. NM_202985.1. [Q9FPS0-2]
UniGeneiAt.3432.

Genome annotation databases

EnsemblPlantsiAT4G39370.1; AT4G39370.1; AT4G39370. [Q9FPS0-1]
GeneIDi830092.
KEGGiath:AT4G39370.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302675 mRNA. Translation: AAG42765.1 .
AL078620 Genomic DNA. Translation: CAB52824.1 . Sequence problems.
AL161595 Genomic DNA. Translation: CAB80600.1 . Sequence problems.
CP002687 Genomic DNA. Translation: AEE87061.1 .
CP002687 Genomic DNA. Translation: AEE87062.1 .
AF370208 mRNA. Translation: AAK44023.1 .
AY133770 mRNA. Translation: AAM91704.1 .
PIRi B85466.
RefSeqi NP_568058.1. NM_120097.2. [Q9FPS0-1 ]
NP_974714.1. NM_202985.1. [Q9FPS0-2 ]
UniGenei At.3432.

3D structure databases

ProteinModelPortali Q9FPS0.
SMRi Q9FPS0. Positions 75-492.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 15372. 1 interaction.
STRINGi 3702.AT4G39370.1-P.

Proteomic databases

PaxDbi Q9FPS0.
PRIDEi Q9FPS0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G39370.1 ; AT4G39370.1 ; AT4G39370 . [Q9FPS0-1 ]
GeneIDi 830092.
KEGGi ath:AT4G39370.

Organism-specific databases

TAIRi AT4G39370.

Phylogenomic databases

eggNOGi COG5533.
HOGENOMi HOG000070653.
InParanoidi Q9FPS0.
KOi K11851.
PhylomeDBi Q9FPS0.

Enzyme and pathway databases

BioCyci ARA:AT4G39370-MONOMER.
ARA:GQT-496-MONOMER.
ARA:GQT-497-MONOMER.

Miscellaneous databases

PROi Q9FPS0.

Gene expression databases

ExpressionAtlasi Q9FPS0. baseline and differential.
Genevestigatori Q9FPS0.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
    Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
    Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE (ISOFORM 1).
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.

Entry informationi

Entry nameiUBP27_ARATH
AccessioniPrimary (citable) accession number: Q9FPS0
Secondary accession number(s): Q3E9N3, Q9SVC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3