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Q9FPS0

- UBP27_ARATH

UniProt

Q9FPS0 - UBP27_ARATH

Protein

Ubiquitin carboxyl-terminal hydrolase 27

Gene

UBP27

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei83 – 831NucleophilePROSITE-ProRule annotation
    Active sitei440 – 4401Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitinyl hydrolase activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciARA:AT4G39370-MONOMER.
    ARA:GQT-496-MONOMER.
    ARA:GQT-497-MONOMER.

    Protein family/group databases

    MEROPSiC19.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 27 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 27
    Short name:
    AtUBP27
    Ubiquitin thioesterase 27
    Ubiquitin-specific-processing protease 27
    Gene namesi
    Name:UBP27
    Ordered Locus Names:At4g39370
    ORF Names:F23K16.5
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G39370.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 494494Ubiquitin carboxyl-terminal hydrolase 27PRO_0000313052Add
    BLAST

    Proteomic databases

    PaxDbiQ9FPS0.
    PRIDEiQ9FPS0.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9FPS0.

    Interactioni

    Protein-protein interaction databases

    BioGridi15372. 1 interaction.
    STRINGi3702.AT4G39370.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FPS0.
    SMRiQ9FPS0. Positions 75-492.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei30 – 5021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini74 – 494421USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 USP domain.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5533.
    HOGENOMiHOG000070653.
    InParanoidiQ9FPS0.
    KOiK11851.
    PhylomeDBiQ9FPS0.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9FPS0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MVSRRGSETK AIVCVLTDRI RISNQWVSHL SFAGLLGVAG FVFAQQHGLF    50
    RNLNNLKLFS GREKDSGDDS FLVPGLQNLG NNCFLNVILQ ALASCKDFRS 100
    FLQWVLEDAR GSLAGEQEEQ LPLTFALSAL LQELGTVGSR RSVSNPRKVM 150
    VTLTDYAKNF NLTSQQDAAE ALLHLISSLQ EEIVVCYRPS QSSNLSDILF 200
    SRNLRMLAPS EGLHGLMELK RWHKHLRGPF DGILGSTLMC RTCSSQISLE 250
    FQFFHTLPLS PLLHHGGYNI MSGCTLEHCL KKFLNTEKVE NYFCYRCWHG 300
    AALKYLSVIG AAETEIEKLR SCGGEDQCDC KTSLHLQRMP WSNSYSHILK 350
    QLIIARFPKL LCIQVQRASF NMFEEFKLSG HIAFPLVLNL SLFTPSSIGV 400
    NIEERIEMSS EYQKPEASKN HGMYRLVTVV EHFGRTGSGH YTVYRSVRVF 450
    SQEEEEEDCD EDLSWFSISD SEVCRVSESD VLGAEASLLF YERL 494
    Length:494
    Mass (Da):55,771
    Last modified:March 1, 2001 - v1
    Checksum:iB8CBDF5799FA9FA6
    GO
    Isoform 2 (identifier: Q9FPS0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         314-361: TEIEKLRSCG...LIIARFPKLL → VVNLSQLLML...AQELWRRGPM
         362-494: Missing.

    Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.

    Show »
    Length:361
    Mass (Da):40,770
    Checksum:iEBD855CB345C1129
    GO

    Sequence cautioni

    The sequence CAB52824.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAB80600.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei314 – 36148TEIEK…FPKLL → VVNLSQLLMLLYFSFVHASR LYLTRVSWKRFQSDGNRKAQ ELWRRGPM in isoform 2. CuratedVSP_029992Add
    BLAST
    Alternative sequencei362 – 494133Missing in isoform 2. CuratedVSP_029993Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302675 mRNA. Translation: AAG42765.1.
    AL078620 Genomic DNA. Translation: CAB52824.1. Sequence problems.
    AL161595 Genomic DNA. Translation: CAB80600.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE87061.1.
    CP002687 Genomic DNA. Translation: AEE87062.1.
    AF370208 mRNA. Translation: AAK44023.1.
    AY133770 mRNA. Translation: AAM91704.1.
    PIRiB85466.
    RefSeqiNP_568058.1. NM_120097.2. [Q9FPS0-1]
    NP_974714.1. NM_202985.1. [Q9FPS0-2]
    UniGeneiAt.3432.

    Genome annotation databases

    EnsemblPlantsiAT4G39370.1; AT4G39370.1; AT4G39370. [Q9FPS0-1]
    GeneIDi830092.
    KEGGiath:AT4G39370.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302675 mRNA. Translation: AAG42765.1 .
    AL078620 Genomic DNA. Translation: CAB52824.1 . Sequence problems.
    AL161595 Genomic DNA. Translation: CAB80600.1 . Sequence problems.
    CP002687 Genomic DNA. Translation: AEE87061.1 .
    CP002687 Genomic DNA. Translation: AEE87062.1 .
    AF370208 mRNA. Translation: AAK44023.1 .
    AY133770 mRNA. Translation: AAM91704.1 .
    PIRi B85466.
    RefSeqi NP_568058.1. NM_120097.2. [Q9FPS0-1 ]
    NP_974714.1. NM_202985.1. [Q9FPS0-2 ]
    UniGenei At.3432.

    3D structure databases

    ProteinModelPortali Q9FPS0.
    SMRi Q9FPS0. Positions 75-492.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 15372. 1 interaction.
    STRINGi 3702.AT4G39370.1-P.

    Protein family/group databases

    MEROPSi C19.002.

    Proteomic databases

    PaxDbi Q9FPS0.
    PRIDEi Q9FPS0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G39370.1 ; AT4G39370.1 ; AT4G39370 . [Q9FPS0-1 ]
    GeneIDi 830092.
    KEGGi ath:AT4G39370.

    Organism-specific databases

    TAIRi AT4G39370.

    Phylogenomic databases

    eggNOGi COG5533.
    HOGENOMi HOG000070653.
    InParanoidi Q9FPS0.
    KOi K11851.
    PhylomeDBi Q9FPS0.

    Enzyme and pathway databases

    BioCyci ARA:AT4G39370-MONOMER.
    ARA:GQT-496-MONOMER.
    ARA:GQT-497-MONOMER.

    Miscellaneous databases

    PROi Q9FPS0.

    Gene expression databases

    Genevestigatori Q9FPS0.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
      Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
      Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE (ISOFORM 1).
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiUBP27_ARATH
    AccessioniPrimary (citable) accession number: Q9FPS0
    Secondary accession number(s): Q3E9N3, Q9SVC0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3