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Protein

Ubiquitin carboxyl-terminal hydrolase 27

Gene

UBP27

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei83NucleophilePROSITE-ProRule annotation1
Active sitei440Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

BioCyciARA:AT4G39370-MONOMER.
ReactomeiR-ATH-5689880. Ub-specific processing proteases.

Protein family/group databases

MEROPSiC19.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 27 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 27
Short name:
AtUBP27
Ubiquitin thioesterase 27
Ubiquitin-specific-processing protease 27
Gene namesi
Name:UBP27
Ordered Locus Names:At4g39370
ORF Names:F23K16.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G39370.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei30 – 50HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003130521 – 494Ubiquitin carboxyl-terminal hydrolase 27Add BLAST494

Proteomic databases

PaxDbiQ9FPS0.

PTM databases

iPTMnetiQ9FPS0.

Expressioni

Gene expression databases

ExpressionAtlasiQ9FPS0. baseline and differential.
GenevisibleiQ9FPS0. AT.

Interactioni

Protein-protein interaction databases

BioGridi15372. 1 interactor.
STRINGi3702.AT4G39370.3.

Structurei

3D structure databases

ProteinModelPortaliQ9FPS0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini74 – 494USPAdd BLAST421

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1868. Eukaryota.
ENOG410XP8T. LUCA.
HOGENOMiHOG000070653.
InParanoidiQ9FPS0.
KOiK11851.
PhylomeDBiQ9FPS0.

Family and domain databases

InterProiView protein in InterPro
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
PfamiView protein in Pfam
PF00443. UCH. 1 hit.
PROSITEiView protein in PROSITE
PS00972. USP_1. 1 hit.
PS50235. USP_3. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9FPS0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVSRRGSETK AIVCVLTDRI RISNQWVSHL SFAGLLGVAG FVFAQQHGLF
60 70 80 90 100
RNLNNLKLFS GREKDSGDDS FLVPGLQNLG NNCFLNVILQ ALASCKDFRS
110 120 130 140 150
FLQWVLEDAR GSLAGEQEEQ LPLTFALSAL LQELGTVGSR RSVSNPRKVM
160 170 180 190 200
VTLTDYAKNF NLTSQQDAAE ALLHLISSLQ EEIVVCYRPS QSSNLSDILF
210 220 230 240 250
SRNLRMLAPS EGLHGLMELK RWHKHLRGPF DGILGSTLMC RTCSSQISLE
260 270 280 290 300
FQFFHTLPLS PLLHHGGYNI MSGCTLEHCL KKFLNTEKVE NYFCYRCWHG
310 320 330 340 350
AALKYLSVIG AAETEIEKLR SCGGEDQCDC KTSLHLQRMP WSNSYSHILK
360 370 380 390 400
QLIIARFPKL LCIQVQRASF NMFEEFKLSG HIAFPLVLNL SLFTPSSIGV
410 420 430 440 450
NIEERIEMSS EYQKPEASKN HGMYRLVTVV EHFGRTGSGH YTVYRSVRVF
460 470 480 490
SQEEEEEDCD EDLSWFSISD SEVCRVSESD VLGAEASLLF YERL
Length:494
Mass (Da):55,771
Last modified:March 1, 2001 - v1
Checksum:iB8CBDF5799FA9FA6
GO
Isoform 2 (identifier: Q9FPS0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     314-361: TEIEKLRSCG...LIIARFPKLL → VVNLSQLLML...AQELWRRGPM
     362-494: Missing.

Note: Derived from EST data. May be due to an intron retention. No experimental confirmation available.
Show »
Length:361
Mass (Da):40,770
Checksum:iEBD855CB345C1129
GO

Sequence cautioni

The sequence CAB52824 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80600 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_029992314 – 361TEIEK…FPKLL → VVNLSQLLMLLYFSFVHASR LYLTRVSWKRFQSDGNRKAQ ELWRRGPM in isoform 2. CuratedAdd BLAST48
Alternative sequenceiVSP_029993362 – 494Missing in isoform 2. CuratedAdd BLAST133

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302675 mRNA. Translation: AAG42765.1.
AL078620 Genomic DNA. Translation: CAB52824.1. Sequence problems.
AL161595 Genomic DNA. Translation: CAB80600.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE87061.1.
CP002687 Genomic DNA. Translation: AEE87062.1.
AF370208 mRNA. Translation: AAK44023.1.
AY133770 mRNA. Translation: AAM91704.1.
PIRiB85466.
RefSeqiNP_001320167.1. NM_001342538.1.
NP_568058.1. NM_120097.3. [Q9FPS0-1]
UniGeneiAt.3432.

Genome annotation databases

EnsemblPlantsiAT4G39370.1; AT4G39370.1; AT4G39370. [Q9FPS0-1]
GeneIDi830092.
GrameneiAT4G39370.1; AT4G39370.1; AT4G39370.
KEGGiath:AT4G39370.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBP27_ARATH
AccessioniPrimary (citable) accession number: Q9FPS0
Secondary accession number(s): Q3E9N3, Q9SVC0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2001
Last modified: August 30, 2017
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families