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Protein

Probable low-specificity L-threonine aldolase 2

Gene

THA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Threonine aldolase involved in threonine degradation to glycine. May play a role in the removal of L-allo-threonine.1 Publication

Catalytic activityi

L-threonine = glycine + acetaldehyde.
L-allo-threonine = glycine + acetaldehyde.

Cofactori

Kineticsi

  1. KM=3.8 mM for L-threonine1 Publication

    Pathwayi: L-threonine degradation via aldolase pathway

    This protein is involved in step 1 of the subpathway that synthesizes acetaldehyde and glycine from L-threonine.
    Proteins known to be involved in this subpathway in this organism are:
    1. Probable low-specificity L-threonine aldolase 2 (THA2), Probable low-specificity L-threonine aldolase 1 (THA1)
    This subpathway is part of the pathway L-threonine degradation via aldolase pathway, which is itself part of Amino-acid degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetaldehyde and glycine from L-threonine, the pathway L-threonine degradation via aldolase pathway and in Amino-acid degradation.

    GO - Molecular functioni

    • L-allo-threonine aldolase activity Source: GO_Central
    • threonine aldolase activity Source: TAIR

    GO - Biological processi

    • glycine biosynthetic process Source: GO_Central
    • threonine catabolic process Source: TAIR

    Keywordsi

    Molecular functionLyase
    LigandPyridoxal phosphate

    Enzyme and pathway databases

    UniPathwayiUPA00044; UER00429

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable low-specificity L-threonine aldolase 2 (EC:4.1.2.48)
    Alternative name(s):
    Threonine aldolase 2
    Gene namesi
    Name:THA2
    Ordered Locus Names:At3g04520
    ORF Names:T27C4.17
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 3

    Organism-specific databases

    AraportiAT3G04520
    TAIRilocus:2100860 AT3G04520

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Pathology & Biotechi

    Disruption phenotypei

    Lethal albino phenotype when homozygous.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004286601 – 355Probable low-specificity L-threonine aldolase 2Add BLAST355

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei211N6-(pyridoxal phosphate)lysineBy similarity1

    Proteomic databases

    PaxDbiQ9FPH3

    Expressioni

    Tissue specificityi

    Expressed in roots, leaf vasculature and flowers.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9FPH3 baseline and differential
    GenevisibleiQ9FPH3 AT

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT3G04520.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FPH3
    SMRiQ9FPH3
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the threonine aldolase family.Curated

    Phylogenomic databases

    eggNOGiKOG1368 Eukaryota
    COG2008 LUCA
    HOGENOMiHOG000219824
    InParanoidiQ9FPH3
    KOiK01620
    OMAiWRHRYGG
    OrthoDBiEOG09360EQZ
    PhylomeDBiQ9FPH3

    Family and domain databases

    Gene3Di3.40.640.10, 1 hit
    3.90.1150.10, 1 hit
    InterProiView protein in InterPro
    IPR001597 ArAA_b-elim_lyase/Thr_aldolase
    IPR015424 PyrdxlP-dep_Trfase
    IPR015422 PyrdxlP-dep_Trfase_dom1
    IPR015421 PyrdxlP-dep_Trfase_major
    IPR023603 Threonine_aldolase
    PANTHERiPTHR10289 PTHR10289, 1 hit
    PfamiView protein in Pfam
    PF01212 Beta_elim_lyase, 1 hit
    PIRSFiPIRSF017617 Thr_aldolase, 1 hit
    SUPFAMiSSF53383 SSF53383, 1 hit

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

    Note: A number of isoforms are produced. According to EST sequences.
    Isoform 1 (identifier: Q9FPH3-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MVTPTTIRTV DLRSDTVTKP TESMRSAMAN AEVDDDVLGN DPTALRLEKE
    60 70 80 90 100
    VAEIAGKEAA MFVPSGTMGN LISVLVHCDE RGSEVILGDD SHIHIYENGG
    110 120 130 140 150
    VSSLGGVHPR TVKNEEDGTM EIGAIEAAVR SPKGDLHHPV TKLICLENTQ
    160 170 180 190 200
    ANCGGRCLPI EYIDKVGELA KKHGLKLHID GARIFNASVA LGVPVKRIVQ
    210 220 230 240 250
    AADSVSICLS KGIGAPVGSV IVGSKKFITK ARWLRKTLGG GMRQIGVLCA
    260 270 280 290 300
    AALVALHENV AKLEDDHKKA RVLAEGLNRI ERLRVNVAAV ETNIIYVDIP
    310 320 330 340 350
    EDPKFGAEEA CKSLEDVGVL VIPQATFRIR IVLHHQISDV DVEYVLSCFE

    KIFHS
    Length:355
    Mass (Da):38,252
    Last modified:March 1, 2001 - v1
    Checksum:iD7D1EFDBA18B517E
    GO

    Sequence cautioni

    The sequence AAF63783 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC022287 Genomic DNA Translation: AAF63783.1 Sequence problems.
    CP002686 Genomic DNA Translation: AEE74091.1
    CP002686 Genomic DNA Translation: ANM65683.1
    AF325033 mRNA Translation: AAG40385.1
    AY091093 mRNA Translation: AAM14044.1
    AY123033 mRNA Translation: AAM67566.1
    AK175345 mRNA Translation: BAD43108.1
    AY085456 mRNA Translation: AAM62682.1
    RefSeqiNP_001327633.1, NM_001337534.1 [Q9FPH3-1]
    NP_566228.1, NM_111323.4 [Q9FPH3-1]
    UniGeneiAt.19145

    Genome annotation databases

    EnsemblPlantsiAT3G04520.1; AT3G04520.1; AT3G04520 [Q9FPH3-1]
    AT3G04520.3; AT3G04520.3; AT3G04520 [Q9FPH3-1]
    GeneIDi819608
    GrameneiAT3G04520.1; AT3G04520.1; AT3G04520 [Q9FPH3-1]
    AT3G04520.3; AT3G04520.3; AT3G04520 [Q9FPH3-1]
    KEGGiath:AT3G04520

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Entry informationi

    Entry nameiTHA2_ARATH
    AccessioniPrimary (citable) accession number: Q9FPH3
    Secondary accession number(s): Q9M835
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: March 1, 2001
    Last modified: April 25, 2018
    This is version 114 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health