Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable low-specificity L-threonine aldolase 2

Gene

THA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Threonine aldolase involved in threonine degradation to glycine. May play a role in the removal of L-allo-threonine.1 Publication

Catalytic activityi

L-threonine = glycine + acetaldehyde.
L-allo-threonine = glycine + acetaldehyde.

Cofactori

Kineticsi

  1. KM=3.8 mM for L-threonine1 Publication

    Pathway: L-threonine degradation via aldolase pathway

    This protein is involved in step 1 of the subpathway that synthesizes acetaldehyde and glycine from L-threonine.
    Proteins known to be involved in this subpathway in this organism are:
    1. Probable low-specificity L-threonine aldolase 2 (THA2), Probable low-specificity L-threonine aldolase 1 (THA1)
    This subpathway is part of the pathway L-threonine degradation via aldolase pathway, which is itself part of Amino-acid degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetaldehyde and glycine from L-threonine, the pathway L-threonine degradation via aldolase pathway and in Amino-acid degradation.

    GO - Molecular functioni

    GO - Biological processi

    • glycine biosynthetic process Source: GO_Central
    • threonine catabolic process Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciARA:GQT-2165-MONOMER.
    UniPathwayiUPA00044; UER00429.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable low-specificity L-threonine aldolase 2 (EC:4.1.2.48)
    Alternative name(s):
    Threonine aldolase 2
    Gene namesi
    Name:THA2
    Ordered Locus Names:At3g04520
    ORF Names:T27C4.17
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548 Componenti: Chromosome 3

    Organism-specific databases

    TAIRiAT3G04520.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Pathology & Biotechi

    Disruption phenotypei

    Lethal albino phenotype when homozygous.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 355355Probable low-specificity L-threonine aldolase 2PRO_0000428660Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei211 – 2111N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    PRIDEiQ9FPH3.

    Expressioni

    Tissue specificityi

    Expressed in roots, leaf vasculature and flowers.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9FPH3. baseline and differential.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT3G04520.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FPH3.
    SMRiQ9FPH3. Positions 10-353.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the threonine aldolase family.Curated

    Phylogenomic databases

    eggNOGiCOG2008.
    HOGENOMiHOG000219824.
    InParanoidiQ9FPH3.
    KOiK01620.
    OMAiEVGDEQI.
    PhylomeDBiQ9FPH3.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR001597. ArAA_b-elim_lyase/Thr_aldolase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR023603. Threonine_aldolase.
    [Graphical view]
    PfamiPF01212. Beta_elim_lyase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017617. Thr_aldolase. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q9FPH3-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MVTPTTIRTV DLRSDTVTKP TESMRSAMAN AEVDDDVLGN DPTALRLEKE
    60 70 80 90 100
    VAEIAGKEAA MFVPSGTMGN LISVLVHCDE RGSEVILGDD SHIHIYENGG
    110 120 130 140 150
    VSSLGGVHPR TVKNEEDGTM EIGAIEAAVR SPKGDLHHPV TKLICLENTQ
    160 170 180 190 200
    ANCGGRCLPI EYIDKVGELA KKHGLKLHID GARIFNASVA LGVPVKRIVQ
    210 220 230 240 250
    AADSVSICLS KGIGAPVGSV IVGSKKFITK ARWLRKTLGG GMRQIGVLCA
    260 270 280 290 300
    AALVALHENV AKLEDDHKKA RVLAEGLNRI ERLRVNVAAV ETNIIYVDIP
    310 320 330 340 350
    EDPKFGAEEA CKSLEDVGVL VIPQATFRIR IVLHHQISDV DVEYVLSCFE

    KIFHS
    Length:355
    Mass (Da):38,252
    Last modified:March 1, 2001 - v1
    Checksum:iD7D1EFDBA18B517E
    GO

    Sequence cautioni

    The sequence AAF63783.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC022287 Genomic DNA. Translation: AAF63783.1. Sequence problems.
    CP002686 Genomic DNA. Translation: AEE74091.1.
    AF325033 mRNA. Translation: AAG40385.1.
    AY091093 mRNA. Translation: AAM14044.1.
    AY123033 mRNA. Translation: AAM67566.1.
    AK175345 mRNA. Translation: BAD43108.1.
    AY085456 mRNA. Translation: AAM62682.1.
    RefSeqiNP_566228.1. NM_111323.3. [Q9FPH3-1]
    UniGeneiAt.19145.

    Genome annotation databases

    EnsemblPlantsiAT3G04520.1; AT3G04520.1; AT3G04520. [Q9FPH3-1]
    GeneIDi819608.
    KEGGiath:AT3G04520.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC022287 Genomic DNA. Translation: AAF63783.1. Sequence problems.
    CP002686 Genomic DNA. Translation: AEE74091.1.
    AF325033 mRNA. Translation: AAG40385.1.
    AY091093 mRNA. Translation: AAM14044.1.
    AY123033 mRNA. Translation: AAM67566.1.
    AK175345 mRNA. Translation: BAD43108.1.
    AY085456 mRNA. Translation: AAM62682.1.
    RefSeqiNP_566228.1. NM_111323.3. [Q9FPH3-1]
    UniGeneiAt.19145.

    3D structure databases

    ProteinModelPortaliQ9FPH3.
    SMRiQ9FPH3. Positions 10-353.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT3G04520.1.

    Proteomic databases

    PRIDEiQ9FPH3.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT3G04520.1; AT3G04520.1; AT3G04520. [Q9FPH3-1]
    GeneIDi819608.
    KEGGiath:AT3G04520.

    Organism-specific databases

    TAIRiAT3G04520.

    Phylogenomic databases

    eggNOGiCOG2008.
    HOGENOMiHOG000219824.
    InParanoidiQ9FPH3.
    KOiK01620.
    OMAiEVGDEQI.
    PhylomeDBiQ9FPH3.

    Enzyme and pathway databases

    UniPathwayiUPA00044; UER00429.
    BioCyciARA:GQT-2165-MONOMER.

    Miscellaneous databases

    PROiQ9FPH3.

    Gene expression databases

    ExpressionAtlasiQ9FPH3. baseline and differential.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR001597. ArAA_b-elim_lyase/Thr_aldolase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR023603. Threonine_aldolase.
    [Graphical view]
    PfamiPF01212. Beta_elim_lyase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017617. Thr_aldolase. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Two Arabidopsis threonine aldolases are nonredundant and compete with threonine deaminase for a common substrate pool."
      Joshi V., Laubengayer K.M., Schauer N., Fernie A.R., Jander G.
      Plant Cell 18:3564-3575(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiTHA2_ARATH
    AccessioniPrimary (citable) accession number: Q9FPH3
    Secondary accession number(s): Q9M835
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: March 1, 2001
    Last modified: June 24, 2015
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.