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Protein

Transcription factor-like protein DPB

Gene

DPB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the regulation of the G1/S transition. Increases the DNA binding activity of E2F proteins after heterodimerization. The complex DPB/E2FC restricts cell division and lateral root initiation and may function as a negative regulator of E2F-regulated genes. The interaction with SKP2A is controlled by auxin.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi101 – 184Sequence analysisAdd BLAST84

GO - Molecular functioni

GO - Biological processi

  • DNA endoreduplication Source: TAIR
  • G1/S transition of mitotic cell cycle Source: TAIR
  • regulation of transcription by RNA polymerase II Source: GO_Central
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processCell cycle, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-ATH-1538133 G0 and Early G1
R-ATH-69231 Cyclin D associated events in G1
R-ATH-8953750 Transcriptional Regulation by E2F6

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor-like protein DPB
Alternative name(s):
DP-like protein B
Short name:
AtDPbB
E2F dimerization partner protein B
Gene namesi
Name:DPB
Synonyms:DP1
Ordered Locus Names:At5g03415
ORF Names:F12E4.160
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G03415
TAIRilocus:1005716177 AT5G03415

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004058651 – 385Transcription factor-like protein DPBAdd BLAST385

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9FNY2

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Developmental stagei

Expressed during the whole cell cycle.1 Publication

Gene expression databases

ExpressionAtlasiQ9FNY2 baseline and differential
GenevisibleiQ9FNY2 AT

Interactioni

Subunit structurei

Heterodimer with non-phosphorylated E2FC. No interaction with phosphorylated E2FC. Interacts preferentially with E2FC, but also with E2FA and E2FB. Interacts with SKP2A. Targeted for proteasomal degradation by the SCF(SKP2A) E3 ubiquitin ligase complex.5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein heterodimerization activity Source: TAIR

Protein-protein interaction databases

BioGridi17123, 21 interactors
IntActiQ9FNY2, 14 interactors
STRINGi3702.AT5G03415.1

Structurei

3D structure databases

ProteinModelPortaliQ9FNY2
SMRiQ9FNY2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili185 – 234Sequence analysisAdd BLAST50

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi150 – 184DEF boxBy similarityAdd BLAST35

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi7 – 21Asn-richAdd BLAST15
Compositional biasi86 – 89Poly-Lys4

Domaini

The DIM domain (182-263) is required for heterodimerization.

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2829 Eukaryota
ENOG410Y9QP LUCA
HOGENOMiHOG000029891
InParanoidiQ9FNY2
OMAiMAMDIIC
OrthoDBiEOG09360FMZ
PhylomeDBiQ9FNY2

Family and domain databases

CDDicd14458 DP_DD, 1 hit
Gene3Di1.10.10.10, 1 hit
1.20.140.80, 1 hit
InterProiView protein in InterPro
IPR037241 E2F-DP_heterodim
IPR003316 E2F_WHTH_DNA-bd_dom
IPR038168 TF_DP_C_sf
IPR014889 Transc_factor_DP_C
IPR015648 Transcrpt_fac_DP
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12548 PTHR12548, 2 hits
PfamiView protein in Pfam
PF08781 DP, 1 hit
PF02319 E2F_TDP, 1 hit
PIRSFiPIRSF009404 Transcription_factor_DP, 1 hit
SMARTiView protein in SMART
SM01138 DP, 1 hit
SM01372 E2F_TDP, 1 hit
SUPFAMiSSF144074 SSF144074, 1 hit
SSF46785 SSF46785, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9FNY2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTTGSNSNH NHHESNNNNN NPSTRSWGTA VSGQSVSTSG SMGSPSSRSE
60 70 80 90 100
QTITVVTSTS DTTFQRLNNL DIQGDDAGSQ GASGVKKKKR GQRAAGPDKT
110 120 130 140 150
GRGLRQFSMK VCEKVESKGR TTYNEVADEL VAEFALPNND GTSPDQQQYD
160 170 180 190 200
EKNIRRRVYD ALNVLMAMDI ISKDKKEIQW RGLPRTSLSD IEELKNERLS
210 220 230 240 250
LRNRIEKKTA YSQELEEQYV GLQNLIQRNE HLYSSGNAPS GGVALPFILV
260 270 280 290 300
QTRPHATVEV EISEDMQLVH FDFNSTPFEL HDDNFVLKTM KFCDQPPQQP
310 320 330 340 350
NGRNNSQLVC HNFTPENPNK GPSTGPTPQL DMYETHLQSQ QHQQHSQLQI
360 370 380
IPMPETNNVT SSADTAPVKS PSLPGIMNSS MKPEN
Length:385
Mass (Da):42,755
Last modified:March 1, 2001 - v1
Checksum:i5DDB4ACA04C52AF8
GO
Isoform 2 (identifier: Q9FNY2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     105-111: RQFSMKV → L

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:379
Mass (Da):41,991
Checksum:i6505E31E4E1F8E1E
GO

Sequence cautioni

The sequence CAB83299 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40G → S in AAM63227 (Ref. 4) Curated1
Sequence conflicti59T → A in AAM63227 (Ref. 4) Curated1
Sequence conflicti303R → Q in AAM63227 (Ref. 4) Curated1
Sequence conflicti375G → R in AAM63227 (Ref. 4) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040734105 – 111RQFSMKV → L in isoform 2. Curated7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ294532 mRNA Translation: CAC15484.1
AL162751 Genomic DNA Translation: CAB83299.1 Sequence problems.
CP002688 Genomic DNA Translation: AED90599.1
CP002688 Genomic DNA Translation: AED90600.1
AY086018 mRNA Translation: AAM63227.1
AK228148 mRNA Translation: BAF00104.1
AB493733 mRNA Translation: BAH30571.1
PIRiT48364
RefSeqiNP_001190214.1, NM_001203285.1 [Q9FNY2-2]
NP_850757.1, NM_180426.3 [Q9FNY2-1]
UniGeneiAt.4864

Genome annotation databases

EnsemblPlantsiAT5G03415.1; AT5G03415.1; AT5G03415 [Q9FNY2-1]
AT5G03415.2; AT5G03415.2; AT5G03415 [Q9FNY2-2]
GeneIDi831847
GrameneiAT5G03415.1; AT5G03415.1; AT5G03415 [Q9FNY2-1]
AT5G03415.2; AT5G03415.2; AT5G03415 [Q9FNY2-2]
KEGGiath:AT5G03415

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDPB_ARATH
AccessioniPrimary (citable) accession number: Q9FNY2
Secondary accession number(s): Q8LDG3, Q9LZE7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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