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Protein

Lipoxygenase 4, chloroplastic

Gene

LOX4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Plant lipoxygenases may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure (By similarity). 13S-lipoxygenase that can use linolenic acid as substrates.PROSITE-ProRule annotation1 Publication

Catalytic activityi

Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit.PROSITE-ProRule annotation

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi585Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi590Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi777Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi781Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi926Iron; via carboxylate; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • linoleate 13S-lipoxygenase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • anther dehiscence Source: TAIR
  • anther development Source: TAIR
  • lipid oxidation Source: TAIR
  • oxylipin biosynthetic process Source: UniProtKB-UniPathway
  • pollen development Source: TAIR
  • response to bacterium Source: UniProtKB
  • response to ozone Source: UniProtKB
  • response to wounding Source: UniProtKB
  • stamen filament development Source: TAIR

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.11.12 399
ReactomeiR-ATH-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
R-ATH-2142696 Synthesis of Hepoxilins (HX) and Trioxilins (TrX)
R-ATH-2142700 Synthesis of Lipoxins (LX)
R-ATH-2142712 Synthesis of 12-eicosatetraenoic acid derivatives
R-ATH-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
R-ATH-6798695 Neutrophil degranulation
R-ATH-9018676 Biosynthesis of D-series resolvins
R-ATH-9018677 Biosynthesis of DHA-derived SPMs
R-ATH-9018681 Biosynthesis of protectins
R-ATH-9018682 Biosynthesis of maresins
R-ATH-9018896 Biosynthesis of E-series 18(S)-resolvins
R-ATH-9020265 Biosynthesis of aspirin-triggered D-series resolvins
R-ATH-9023661 Biosynthesis of E-series 18(R)-resolvins
R-ATH-9025106 Biosynthesis of DPAn-6 SPMs
R-ATH-9026286 Biosynthesis of DPAn-3-derived protectins and resolvins
R-ATH-9026290 Biosynthesis of DPAn-3-derived maresins
R-ATH-9026403 Biosynthesis of DPAn-3-derived 13-series resolvins
R-ATH-9027604 Biosynthesis of electrophilic Omega-3 PUFA oxo-derivatives
UniPathwayiUPA00382

Chemistry databases

SwissLipidsiSLP:000001765

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoxygenase 4, chloroplastic (EC:1.13.11.12)
Short name:
AtLOX4
Alternative name(s):
LOX3-like protein
Gene namesi
Name:LOX4
Synonyms:LOX3
Ordered Locus Names:At1g72520
ORF Names:F28P22.29, T10D10.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G72520
TAIRilocus:2030215 AT1G72520

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 58ChloroplastSequence analysisAdd BLAST58
ChainiPRO_000038059359 – 926Lipoxygenase 4, chloroplasticAdd BLAST868

Proteomic databases

PaxDbiQ9FNX8
PRIDEiQ9FNX8

Expressioni

Tissue specificityi

Expressed in leaves.1 Publication

Developmental stagei

Expression is greatly increased in leaves during leaf senescence.1 Publication

Inductioni

Induced by bacterial pathogens (e.g. Pseudomonas syringae pv. tomato), ozone O3, and wounding.2 Publications

Gene expression databases

ExpressionAtlasiQ9FNX8 baseline and differential
GenevisibleiQ9FNX8 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G72520.1

Structurei

3D structure databases

ProteinModelPortaliQ9FNX8
SMRiQ9FNX8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini106 – 228PLATPROSITE-ProRule annotationAdd BLAST123
Domaini231 – 926LipoxygenasePROSITE-ProRule annotationAdd BLAST696

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IEKP Eukaryota
ENOG410YN4N LUCA
HOGENOMiHOG000230469
InParanoidiQ9FNX8
KOiK00454
OMAiKDHPSKR
OrthoDBiEOG0936038B
PhylomeDBiQ9FNX8

Family and domain databases

Gene3Di4.10.372.10, 1 hit
InterProiView protein in InterPro
IPR000907 LipOase
IPR013819 LipOase_C
IPR036226 LipOase_C_sf
IPR020834 LipOase_CS
IPR020833 LipOase_Fe_BS
IPR001246 LipOase_plant
IPR027433 Lipoxygenase_dom_3
IPR001024 PLAT/LH2_dom
IPR036392 PLAT/LH2_dom_sf
PANTHERiPTHR11771 PTHR11771, 1 hit
PfamiView protein in Pfam
PF00305 Lipoxygenase, 1 hit
PF01477 PLAT, 1 hit
PRINTSiPR00087 LIPOXYGENASE
PR00468 PLTLPOXGNASE
SMARTiView protein in SMART
SM00308 LH2, 1 hit
SUPFAMiSSF48484 SSF48484, 1 hit
SSF49723 SSF49723, 1 hit
PROSITEiView protein in PROSITE
PS00711 LIPOXYGENASE_1, 1 hit
PS00081 LIPOXYGENASE_2, 1 hit
PS51393 LIPOXYGENASE_3, 1 hit
PS50095 PLAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FNX8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALANEIMGS RLIFERSSSL ASPFHSRFSI KKKTQRTQFS INPFDPRPMR
60 70 80 90 100
AVNSSGVVAA ISEDLVKTLR ISTVGRKQEK EEEEEKSVKF KVRAVATVRN
110 120 130 140 150
KNKEDFKETL VKHLDAFTDK IGRNVVLELM STQVDPKTNE PKKSKAAVLK
160 170 180 190 200
DWSKKSNSKA ERVHYTAEFT VDSAFGSPGA ITVTNKHQKE FFLESITIEG
210 220 230 240 250
FACGPVHFPC NSWVQSQKDH PSKRILFTNQ PYLPSETPSG LRTLREKELE
260 270 280 290 300
NLRGNGKGER KLSDRIYDYD VYNDIGNPDI SRELARPTLG GREFPYPRRC
310 320 330 340 350
RTGRSSTDTD MMSERRVEKP LPMYVPRDEQ FEESKQNTFA ACRLKAVLHN
360 370 380 390 400
LIPSLKASIL AEDFANFGEI DSLYKEGLLL KLGFQDDMFK KFPLPKIVTT
410 420 430 440 450
LQKSSEGLLR YDTPKIVSKD KYAWLRDDEF ARQAIAGINP VNIERVTSYP
460 470 480 490 500
PVSNLDPEIY GPGLHSALTE DHIIGQLDGL TVQQALETNR LFMVDYHDIY
510 520 530 540 550
LPFLDRINAL DGRKAYATRT ILFLTRLGTL KPIAIELSLP SQSSSNQKSK
560 570 580 590 600
RVVTPPVDAT SNWMWQLAKA HVGSNDAGVH QLVNHWLRTH ACLEPFILAA
610 620 630 640 650
HRQLSAMHPI FKLLDPHMRY TLEINAVARQ TLISADGVIE SCFTAGQYGL
660 670 680 690 700
EISSAAYKNK WRFDMEGLPA DLIRRGMAVP DPTQPHGLKL LVEDYPYAND
710 720 730 740 750
GLLLWSAIQT WVRTYVERYY ANSNLIQTDT ELQAWYSESI NVGHADHRDA
760 770 780 790 800
EWWPKLSTVE DLVSVITTII WLASAQHAAL NFGQYPYGGY VPNRPPLMRR
810 820 830 840 850
LIPDESDPEF TSFIEDPQKY FFSSMPSLLQ TTKFMAVVDT LSTHSPDEEY
860 870 880 890 900
IGERQQPSIW TGDAEIVDAF YGFSAEIGRI EKEIDKRNRD PSRRNRCGAG
910 920
VLPYELMAPS SEPGVTCRGV PNSVSI
Length:926
Mass (Da):104,814
Last modified:March 1, 2001 - v1
Checksum:i3592FB6ECDCCC4A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ302042 mRNA Translation: CAC19364.1
AC010926 Genomic DNA Translation: AAG51846.1
AC016529 Genomic DNA Translation: AAG52571.1
CP002684 Genomic DNA Translation: AEE35334.1
AY056166 mRNA Translation: AAL07015.1
AY091193 mRNA Translation: AAM14132.1
PIRiE96749
RefSeqiNP_177396.1, NM_105911.5
UniGeneiAt.18241
At.67292

Genome annotation databases

EnsemblPlantsiAT1G72520.1; AT1G72520.1; AT1G72520
GeneIDi843584
GrameneiAT1G72520.1; AT1G72520.1; AT1G72520
KEGGiath:AT1G72520

Similar proteinsi

Entry informationi

Entry nameiLOX4_ARATH
AccessioniPrimary (citable) accession number: Q9FNX8
Secondary accession number(s): Q9CAG9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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