Reviewed, UniProtKB/Swiss-Prot Q9FNQ2 (E13L2_ARATH)

Names and origin

Protein names

Recommended name:
    Glucan endo-1,3-beta-glucosidase-like protein 2

Gene names

Ordered Locus Names:	At5g61130
ORF Names:	MAF19.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location

Cell membrane; Lipid-anchor › GPI-anchor. Ref.3 Ref.4

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	callose deposition in cell wall Inferred from mutant phenotype. Source: TAIR
Cellular component	anchored to plasma membrane Ref.3 Inferred from direct assay. Source: TAIR plant-type cell wall Inferred from direct assay. Source: TAIR plasmodesma Inferred from direct assay. Source: TAIR
Molecular function	polysaccharide binding Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 172	153	Glucan endo-1,3-beta-glucosidase-like protein 2		PRO_0000254001
	Propeptide	173 – 201	29	Removed in mature form Potential		PRO_0000254002
Regions
	Compositional bias	89 – 170	82	Thr-rich
Amino acid modifications
	Lipidation	172	1	GPI-anchor amidated serine Potential
	Glycosylation	154	1	N-linked (GlcNAc...) Potential
	Glycosylation	179	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9FNQ2-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: D7DD73D40B9F0930	FASTA	201	20,365
	10 20 30 40 50 60 MAALVLSLLL LSLAGHSSAS WCVCKTGLSD TVLQATLDYA CGNGADCNPT KPKQSCFNPD 70 80 90 100 110 120 NVRSHCNYAV NSFFQKKGQS PGSCNFDGTA TPTNSDPSYT GCAFPTSASG SSGSTTVTPG 130 140 150 160 170 180 TTNPKGSPTT TTLPGSGTNS PYSGNPTNGV FGGNSTGGTT GTGINPDYTT DSSAFALKNS 190 200 SKLFICLLLI ASSGFCSFLM L

References

[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones." Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:291-300(1997) [PubMed: 9405937] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins." Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N. Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed: 14517339] [Abstract] Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[4]	"Modification-specific proteomics of plasma membrane proteins: identification and characterization of glycosylphosphatidylinositol-anchored proteins released upon phospholipase D treatment." Elortza F., Mohammed S., Bunkenborg J., Foster L.J., Nuehse T.S., Brodbeck U., Peck S.C., Jensen O.N. J. Proteome Res. 5:935-943(2006) [PubMed: 16602701] [Abstract] Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB006696 Genomic DNA. Translation: BAB10375.1. BT005195 mRNA. Translation: AAO50728.1. BT003876 mRNA. Translation: AAO41925.1.
IPI	IPI00528246.
RefSeq	NP_200921.2.
UniGene	At.29122
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	CBM43. Carbohydrate-Binding Module Family 43.
Proteomic databases
PRIDE	Q9FNQ2.
Genome annotation databases
GeneID	836234.
GenomeReviews	Gene locus AT5G61130 in contig BA000015_GR.
KEGG	ath:AT5G61130.
NMPDR	fig|3702.1.peg.28131.
Organism-specific databases
TAIR	At5g61130.
Phylogenomic databases
OMA	Q9FNQ2. PDSSEAP.
Gene expression databases
ArrayExpress	Q9FNQ2.
GermOnline	AT5G61130. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR012946. X8. [Graphical view]
Pfam	PF07983. X8. 1 hit. [Graphical view]
SMART	SM00768. X8. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13L2_ARATH

Accession

Primary (citable) accession number: Q9FNQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)