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Protein

NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

Gene

At5g08530

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:
  • FMNCuratedNote: Binds 1 FMN.Curated
  • [4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi402 – 4021Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi405 – 4051Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi408 – 4081Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi448 – 4481Iron-sulfur (4Fe-4S)Sequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi110 – 11910NAD(H)By similarity
Nucleotide bindingi222 – 27049FMNBy similarityAdd
BLAST

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. FMN binding Source: InterPro
  3. metal ion binding Source: UniProtKB-KW
  4. NAD binding Source: InterPro
  5. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BioCyciARA:AT5G08530-MONOMER.
MetaCyc:AT5G08530-MONOMER.

Protein family/group databases

TCDBi3.D.1.6.3. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Gene namesi
Ordered Locus Names:At5g08530
ORF Names:MAH20.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G08530.

Subcellular locationi

Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Matrix side By similarity

GO - Cellular componenti

  1. mitochondrial respiratory chain complex I Source: TAIR
  2. mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3030MitochondrionSequence AnalysisAdd
BLAST
Chaini31 – 486456NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrialPRO_0000410789Add
BLAST

Proteomic databases

PRIDEiQ9FNN5.
ProMEXiQ9FNN5.

Expressioni

Gene expression databases

GenevestigatoriQ9FNN5.

Interactioni

Subunit structurei

Complex I is composed of at least 49 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.

Protein-protein interaction databases

IntActiQ9FNN5. 2 interactions.
STRINGi3702.AT5G08530.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FNN5.
SMRiQ9FNN5. Positions 80-476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi41 – 5010Pro-rich

Sequence similaritiesi

Belongs to the complex I 51 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1894.
HOGENOMiHOG000251534.
InParanoidiQ9FNN5.
KOiK03942.
OMAiNEPCTVE.
PhylomeDBiQ9FNN5.

Family and domain databases

InterProiIPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
IPR011537. NADH-UbQ_OxRdtase_suF.
IPR011538. Nuo51_FMN-bd.
IPR019575. Nuop51_4Fe4S-bd.
IPR019554. Soluble_ligand-bd.
[Graphical view]
PfamiPF01512. Complex1_51K. 1 hit.
PF10589. NADH_4Fe-4S. 1 hit.
PF10531. SLBB. 1 hit.
[Graphical view]
SMARTiSM00928. NADH_4Fe-4S. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01959. nuoF_fam. 1 hit.
PROSITEiPS00644. COMPLEX1_51K_1. 1 hit.
PS00645. COMPLEX1_51K_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FNN5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPVRGILGL QRAVSIWKES NRLTPALRSF STQAASTSTT PQPPPPPPPP
60 70 80 90 100
EKTHFGGLKD EDRIFTNLYG LHDPFLKGAM KRGDWHRTKD LVLKGTDWIV
110 120 130 140 150
NEMKKSGLRG RGGAGFPSGL KWSFMPKVSD GRPSYLVVNA DESEPGTCKD
160 170 180 190 200
REIMRHDPHK LLEGCLIAGV GMRASAAYIY IRGEYVNERL NLEKARREAY
210 220 230 240 250
AAGLLGKNAC GSGYDFEVYI HFGAGAYICG EETALLESLE GKQGKPRLKP
260 270 280 290 300
PFPANAGLYG CPTTVTNVET VAVSPTILRR GPEWFSSFGR KNNAGTKLFC
310 320 330 340 350
ISGHVNKPCT VEEEMSIPLK ELIERHCGGV RGGWDNLLAI IPGGSSVPLI
360 370 380 390 400
PKNICEDVLM DFDALKAVQS GLGTAAVIVM DKSTDVVDAI ARLSYFYKHE
410 420 430 440 450
SCGQCTPCRE GTGWLWMIME RMKVGNAKLE EIDMLQEVTK QIEGHTICAL
460 470 480
GDAAAWPVQG LIRHFRPELE RRIRERAERE LLQAAA
Length:486
Mass (Da):53,449
Last modified:March 1, 2001 - v1
Checksum:iDC751ADE75F7E9CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006697 Genomic DNA. Translation: BAB10002.1.
CP002688 Genomic DNA. Translation: AED91315.1.
AY092971 mRNA. Translation: AAM12970.1.
BT006620 mRNA. Translation: AAP31964.1.
AY087737 mRNA. Translation: AAM65274.1.
RefSeqiNP_196470.1. NM_120938.3.
UniGeneiAt.8763.

Genome annotation databases

EnsemblPlantsiAT5G08530.1; AT5G08530.1; AT5G08530.
GeneIDi830752.
KEGGiath:AT5G08530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006697 Genomic DNA. Translation: BAB10002.1.
CP002688 Genomic DNA. Translation: AED91315.1.
AY092971 mRNA. Translation: AAM12970.1.
BT006620 mRNA. Translation: AAP31964.1.
AY087737 mRNA. Translation: AAM65274.1.
RefSeqiNP_196470.1. NM_120938.3.
UniGeneiAt.8763.

3D structure databases

ProteinModelPortaliQ9FNN5.
SMRiQ9FNN5. Positions 80-476.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9FNN5. 2 interactions.
STRINGi3702.AT5G08530.1-P.

Protein family/group databases

TCDBi3.D.1.6.3. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PRIDEiQ9FNN5.
ProMEXiQ9FNN5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G08530.1; AT5G08530.1; AT5G08530.
GeneIDi830752.
KEGGiath:AT5G08530.

Organism-specific databases

GeneFarmi1810.
TAIRiAT5G08530.

Phylogenomic databases

eggNOGiCOG1894.
HOGENOMiHOG000251534.
InParanoidiQ9FNN5.
KOiK03942.
OMAiNEPCTVE.
PhylomeDBiQ9FNN5.

Enzyme and pathway databases

BioCyciARA:AT5G08530-MONOMER.
MetaCyc:AT5G08530-MONOMER.

Miscellaneous databases

PROiQ9FNN5.

Gene expression databases

GenevestigatoriQ9FNN5.

Family and domain databases

InterProiIPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
IPR011537. NADH-UbQ_OxRdtase_suF.
IPR011538. Nuo51_FMN-bd.
IPR019575. Nuop51_4Fe4S-bd.
IPR019554. Soluble_ligand-bd.
[Graphical view]
PfamiPF01512. Complex1_51K. 1 hit.
PF10589. NADH_4Fe-4S. 1 hit.
PF10531. SLBB. 1 hit.
[Graphical view]
SMARTiSM00928. NADH_4Fe-4S. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01959. nuoF_fam. 1 hit.
PROSITEiPS00644. COMPLEX1_51K_1. 1 hit.
PS00645. COMPLEX1_51K_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones."
    Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 4:291-300(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiNDUV1_ARATH
AccessioniPrimary (citable) accession number: Q9FNN5
Secondary accession number(s): Q8LAL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: March 1, 2001
Last modified: January 7, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.