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Q9FNN5

- NDUV1_ARATH

UniProt

Q9FNN5 - NDUV1_ARATH

Protein

NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

Gene

At5g08530

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.By similarity

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 1 FMN.Curated
    Binds 1 4Fe-4S cluster.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi402 – 4021Iron-sulfur (4Fe-4S)Sequence Analysis
    Metal bindingi405 – 4051Iron-sulfur (4Fe-4S)Sequence Analysis
    Metal bindingi408 – 4081Iron-sulfur (4Fe-4S)Sequence Analysis
    Metal bindingi448 – 4481Iron-sulfur (4Fe-4S)Sequence Analysis

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi110 – 11910NAD(H)By similarity
    Nucleotide bindingi222 – 27049FMNBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. FMN binding Source: InterPro
    3. metal ion binding Source: UniProtKB-KW
    4. NAD binding Source: InterPro
    5. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    4Fe-4S, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Enzyme and pathway databases

    BioCyciARA:AT5G08530-MONOMER.
    MetaCyc:AT5G08530-MONOMER.

    Protein family/group databases

    TCDBi3.D.1.6.3. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Gene namesi
    Ordered Locus Names:At5g08530
    ORF Names:MAH20.9
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G08530.

    Subcellular locationi

    Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Matrix side By similarity

    GO - Cellular componenti

    1. mitochondrial respiratory chain complex I Source: TAIR
    2. mitochondrion Source: TAIR

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3030MitochondrionSequence AnalysisAdd
    BLAST
    Chaini31 – 486456NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrialPRO_0000410789Add
    BLAST

    Proteomic databases

    PRIDEiQ9FNN5.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9FNN5.

    Interactioni

    Subunit structurei

    Complex I is composed of at least 49 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.

    Protein-protein interaction databases

    IntActiQ9FNN5. 2 interactions.
    STRINGi3702.AT5G08530.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FNN5.
    SMRiQ9FNN5. Positions 80-476.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi41 – 5010Pro-rich

    Sequence similaritiesi

    Belongs to the complex I 51 kDa subunit family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1894.
    HOGENOMiHOG000251534.
    InParanoidiQ9FNN5.
    KOiK03942.
    OMAiKWSFMNP.
    PhylomeDBiQ9FNN5.

    Family and domain databases

    InterProiIPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
    IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd.
    IPR011537. NADH-UbQ_OxRdtase_suF.
    IPR011538. NADH_UbQ_OxRdtase_51kDa_su.
    IPR019554. Soluble_ligand-bd.
    [Graphical view]
    PfamiPF01512. Complex1_51K. 1 hit.
    PF10589. NADH_4Fe-4S. 1 hit.
    PF10531. SLBB. 1 hit.
    [Graphical view]
    SMARTiSM00928. NADH_4Fe-4S. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01959. nuoF_fam. 1 hit.
    PROSITEiPS00644. COMPLEX1_51K_1. 1 hit.
    PS00645. COMPLEX1_51K_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9FNN5-1 [UniParc]FASTAAdd to Basket

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    MAPVRGILGL QRAVSIWKES NRLTPALRSF STQAASTSTT PQPPPPPPPP    50
    EKTHFGGLKD EDRIFTNLYG LHDPFLKGAM KRGDWHRTKD LVLKGTDWIV 100
    NEMKKSGLRG RGGAGFPSGL KWSFMPKVSD GRPSYLVVNA DESEPGTCKD 150
    REIMRHDPHK LLEGCLIAGV GMRASAAYIY IRGEYVNERL NLEKARREAY 200
    AAGLLGKNAC GSGYDFEVYI HFGAGAYICG EETALLESLE GKQGKPRLKP 250
    PFPANAGLYG CPTTVTNVET VAVSPTILRR GPEWFSSFGR KNNAGTKLFC 300
    ISGHVNKPCT VEEEMSIPLK ELIERHCGGV RGGWDNLLAI IPGGSSVPLI 350
    PKNICEDVLM DFDALKAVQS GLGTAAVIVM DKSTDVVDAI ARLSYFYKHE 400
    SCGQCTPCRE GTGWLWMIME RMKVGNAKLE EIDMLQEVTK QIEGHTICAL 450
    GDAAAWPVQG LIRHFRPELE RRIRERAERE LLQAAA 486
    Length:486
    Mass (Da):53,449
    Last modified:March 1, 2001 - v1
    Checksum:iDC751ADE75F7E9CE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB006697 Genomic DNA. Translation: BAB10002.1.
    CP002688 Genomic DNA. Translation: AED91315.1.
    AY092971 mRNA. Translation: AAM12970.1.
    BT006620 mRNA. Translation: AAP31964.1.
    AY087737 mRNA. Translation: AAM65274.1.
    RefSeqiNP_196470.1. NM_120938.3.
    UniGeneiAt.8763.

    Genome annotation databases

    EnsemblPlantsiAT5G08530.1; AT5G08530.1; AT5G08530.
    GeneIDi830752.
    KEGGiath:AT5G08530.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB006697 Genomic DNA. Translation: BAB10002.1 .
    CP002688 Genomic DNA. Translation: AED91315.1 .
    AY092971 mRNA. Translation: AAM12970.1 .
    BT006620 mRNA. Translation: AAP31964.1 .
    AY087737 mRNA. Translation: AAM65274.1 .
    RefSeqi NP_196470.1. NM_120938.3.
    UniGenei At.8763.

    3D structure databases

    ProteinModelPortali Q9FNN5.
    SMRi Q9FNN5. Positions 80-476.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9FNN5. 2 interactions.
    STRINGi 3702.AT5G08530.1-P.

    Protein family/group databases

    TCDBi 3.D.1.6.3. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Proteomic databases

    PRIDEi Q9FNN5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G08530.1 ; AT5G08530.1 ; AT5G08530 .
    GeneIDi 830752.
    KEGGi ath:AT5G08530.

    Organism-specific databases

    GeneFarmi 1810.
    TAIRi AT5G08530.

    Phylogenomic databases

    eggNOGi COG1894.
    HOGENOMi HOG000251534.
    InParanoidi Q9FNN5.
    KOi K03942.
    OMAi KWSFMNP.
    PhylomeDBi Q9FNN5.

    Enzyme and pathway databases

    BioCyci ARA:AT5G08530-MONOMER.
    MetaCyc:AT5G08530-MONOMER.

    Miscellaneous databases

    PROi Q9FNN5.

    Gene expression databases

    Genevestigatori Q9FNN5.

    Family and domain databases

    InterProi IPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
    IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd.
    IPR011537. NADH-UbQ_OxRdtase_suF.
    IPR011538. NADH_UbQ_OxRdtase_51kDa_su.
    IPR019554. Soluble_ligand-bd.
    [Graphical view ]
    Pfami PF01512. Complex1_51K. 1 hit.
    PF10589. NADH_4Fe-4S. 1 hit.
    PF10531. SLBB. 1 hit.
    [Graphical view ]
    SMARTi SM00928. NADH_4Fe-4S. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01959. nuoF_fam. 1 hit.
    PROSITEi PS00644. COMPLEX1_51K_1. 1 hit.
    PS00645. COMPLEX1_51K_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones."
      Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 4:291-300(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiNDUV1_ARATH
    AccessioniPrimary (citable) accession number: Q9FNN5
    Secondary accession number(s): Q8LAL7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 28, 2011
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3