NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial precursor

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Q9FNN5 (NDUV1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 67. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
EC=1.6.5.3
EC=1.6.99.3

Gene names

Ordered Locus Names:	At5g08530
ORF Names:	MAH20.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD⁺ + ubiquinol. NADH + acceptor = NAD⁺ + reduced acceptor.
Cofactor	Binds 1 FMN Potential. Binds 1 4Fe-4S cluster Potential.
Subunit structure	Complex I is composed of at least 49 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side By similarity.
Sequence similarities	Belongs to the complex I 51 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrial respiratory chain complex I Inferred from direct assay PubMed 18189341 PubMed 20197505. Source: TAIR
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 30

Mitochondrion Potential

Chain

31 – 486

456

NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

PRO_0000410789

Regions

Nucleotide binding

110 – 119

NAD(H) By similarity

Nucleotide binding

222 – 270

FMN By similarity

Compositional bias

41 – 50

Pro-rich

Sites

Metal binding

402

Iron-sulfur (4Fe-4S) Potential

Metal binding

405

Iron-sulfur (4Fe-4S) Potential

Metal binding

408

Iron-sulfur (4Fe-4S) Potential

Metal binding

448

Iron-sulfur (4Fe-4S) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q9FNN5 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: DC751ADE75F7E9CE
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FASTA

486

53,449

        10         20         30         40         50         60 
MAPVRGILGL QRAVSIWKES NRLTPALRSF STQAASTSTT PQPPPPPPPP EKTHFGGLKD 

        70         80         90        100        110        120 
EDRIFTNLYG LHDPFLKGAM KRGDWHRTKD LVLKGTDWIV NEMKKSGLRG RGGAGFPSGL 

       130        140        150        160        170        180 
KWSFMPKVSD GRPSYLVVNA DESEPGTCKD REIMRHDPHK LLEGCLIAGV GMRASAAYIY 

       190        200        210        220        230        240 
IRGEYVNERL NLEKARREAY AAGLLGKNAC GSGYDFEVYI HFGAGAYICG EETALLESLE 

       250        260        270        280        290        300 
GKQGKPRLKP PFPANAGLYG CPTTVTNVET VAVSPTILRR GPEWFSSFGR KNNAGTKLFC 

       310        320        330        340        350        360 
ISGHVNKPCT VEEEMSIPLK ELIERHCGGV RGGWDNLLAI IPGGSSVPLI PKNICEDVLM 

       370        380        390        400        410        420 
DFDALKAVQS GLGTAAVIVM DKSTDVVDAI ARLSYFYKHE SCGQCTPCRE GTGWLWMIME 

       430        440        450        460        470        480 
RMKVGNAKLE EIDMLQEVTK QIEGHTICAL GDAAAWPVQG LIRHFRPELE RRIRERAERE 


LLQAAA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones." Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:291-300(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB006697 Genomic DNA. Translation: BAB10002.1. CP002688 Genomic DNA. Translation: AED91315.1. AY092971 mRNA. Translation: AAM12970.1. BT006620 mRNA. Translation: AAP31964.1. AY087737 mRNA. Translation: AAM65274.1.
IPI	IPI00521150.
RefSeq	NP_196470.1. NM_120938.3.
UniGene	At.8763.
3D structure databases
ProteinModelPortal	Q9FNN5.
SMR	Q9FNN5. Positions 80-476.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9FNN5. 2 interactions.
STRING	3702.AT5G08530.1-P.
Protein family/group databases
TCDB	3.D.1.6.3. H+ or Na+-translocating NADH dehydrogenase (NDH) family.
Proteomic databases
PRIDE	Q9FNN5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	830752.
KEGG	ath:AT5G08530.
Organism-specific databases
GeneFarm	1810.
TAIR	At5g08530.
Phylogenomic databases
eggNOG	COG1894.
HOGENOM	HOG000251534.
InParanoid	Q9FNN5.
KO	K03942.
OMA	PSGMKWS.
PhylomeDB	Q9FNN5.
ProtClustDB	PLN03132.
Enzyme and pathway databases
BioCyc	ARA:AT5G08530-MONOMER. MetaCyc:AT5G08530-MONOMER.
Gene expression databases
Genevestigator	Q9FNN5.
Family and domain databases
InterPro	IPR001949. NADH-UbQ_OxRdtase_51kDa_CS. IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd. IPR011537. NADH-UbQ_OxRdtase_suF. IPR011538. NADH_UbQ_OxRdtase_51kDa_su. IPR019554. Soluble_ligand-bd. [Graphical view]
Pfam	PF01512. Complex1_51K. 1 hit. PF10589. NADH_4Fe-4S. 1 hit. PF10531. SLBB. 1 hit. [Graphical view]
SMART	SM00928. NADH_4Fe-4S. 1 hit. [Graphical view]
TIGRFAMs	TIGR01959. nuoF_fam. 1 hit.
PROSITE	PS00644. COMPLEX1_51K_1. 1 hit. PS00645. COMPLEX1_51K_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUV1_ARATH

Accession

Primary (citable) accession number: Q9FNN5
Secondary accession number(s): Q8LAL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents