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Protein

Ornithine aminotransferase, mitochondrial

Gene

DELTA-OAT

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates degradation of arginine for nitrogen recycling. Plays a role in non-host disease resistance by regulating pyrroline-5-carboxylate metabolism-induced hypersensitive response.2 Publications

Catalytic activityi

L-ornithine + a 2-oxo acid = L-glutamate 5-semialdehyde + an L-amino acid.

Cofactori

Kineticsi

The activity was enhanced in young plants under salt-stress conditions.

  1. KM=91 mM for L-ornithine1 Publication
  1. Vmax=28 µmol/h/mg enzyme toward L-glutamate 5-semialdehyde1 Publication

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-ornithine.
Proteins known to be involved in this subpathway in this organism are:
  1. Ornithine aminotransferase, mitochondrial (DELTA-OAT)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-ornithine, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • identical protein binding Source: GO_Central
  • ornithine-oxo-acid transaminase activity Source: UniProtKB
  • pyridoxal phosphate binding Source: GO_Central
  • zinc ion binding Source: TAIR

GO - Biological processi

  • arginine catabolic process to glutamate Source: TAIR
  • arginine catabolic process to proline via ornithine Source: GO_Central
  • defense response to bacterium, incompatible interaction Source: UniProtKB
  • hyperosmotic salinity response Source: TAIR
  • mitochondrion localization Source: CACAO
  • ornithine catabolic process Source: UniProtKB
  • plant-type hypersensitive response Source: UniProtKB
  • proline biosynthetic process Source: TAIR
  • response to salt stress Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Plant defense

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT5G46180-MONOMER.
MetaCyc:AT5G46180-MONOMER.
BRENDAi2.6.1.13. 399.
ReactomeiR-ATH-70614. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00098; UER00358.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine aminotransferase, mitochondrial (EC:2.6.1.13)
Alternative name(s):
Ornithine delta-aminotransferase
Ornithine--oxo-acid aminotransferase
Gene namesi
Name:DELTA-OAT
Ordered Locus Names:At5g46180
ORF Names:MCL19.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G46180.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Unable to use arginine or ornithine as nitrogen source. Displays sensitivity against type II non-host pathogen infection.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1616MitochondrionSequence analysisAdd
BLAST
Chaini17 – 475459Ornithine aminotransferase, mitochondrialPRO_0000421298Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei294 – 2941N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

PaxDbiQ9FNK4.
PRIDEiQ9FNK4.

PTM databases

iPTMnetiQ9FNK4.
SwissPalmiQ9FNK4.

Expressioni

Inductioni

By salt stress in young plants. Up-regulated during low water potential. Induced upon non-host pathogen infection.3 Publications

Gene expression databases

GenevisibleiQ9FNK4. AT.

Interactioni

Subunit structurei

Homotetramer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT5G46180.1.

Structurei

3D structure databases

ProteinModelPortaliQ9FNK4.
SMRiQ9FNK4. Positions 37-443.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1402. Eukaryota.
COG4992. LUCA.
HOGENOMiHOG000020206.
InParanoidiQ9FNK4.
KOiK00819.
OMAiTHDHTIR.
OrthoDBiEOG093609WJ.
PhylomeDBiQ9FNK4.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR005814. Aminotrans_3.
IPR010164. Orn_aminotrans.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PTHR11986:SF18. PTHR11986:SF18. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01885. Orn_aminotrans. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FNK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATTRRLLY YVSKRFSTAG VRRSYGGLPQ SNSKSPPSSS QRLMELESEF
60 70 80 90 100
SAHNYHPVPV VFSRANGSTI WDPEGKRYID FLAAYSAVNQ GHCHPKIMKA
110 120 130 140 150
LQEQVEKLTL SSRAFYNDKF PVFAERLTNM FGYDMVLPMN TGAEGVETAL
160 170 180 190 200
KLARKWGHEK KNIPKDEAII VSCCGCFHGR TLAIVSMSCD NDATRGFGPL
210 220 230 240 250
LPGNLKVDFG DADSLEKIFK EKGDRIAGFL FEPIQGEAGV IIPPDGYLKA
260 270 280 290 300
VRELCTKYNV LMIADEVQSG LARSGKMLAC DWEEIRPDMV ILGKALGGGV
310 320 330 340 350
IPVSAVLADK DVMLHIKPGQ HGSTFGGNPL ASAVAMASLD VIVEEKLVER
360 370 380 390 400
SASLGEELRI QLNEIKKQFP KYIKEVRGRG LFNAIEFNSE SLSPVSAYDI
410 420 430 440 450
CLSLKERGVL AKPTHNTIVR LTPPLSISSD ELRDGSEALH DVLELDLPNL
460 470
LKINSGKTPV SHITECDRCG RNLYA
Length:475
Mass (Da):52,178
Last modified:March 1, 2001 - v1
Checksum:iA886C999AB0B68CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006698 Genomic DNA. Translation: BAB08263.1.
CP002688 Genomic DNA. Translation: AED95350.1.
BT023421 mRNA. Translation: AAY56412.1.
BT029160 mRNA. Translation: ABJ17095.1.
RefSeqiNP_199430.1. NM_123987.3.
UniGeneiAt.28104.

Genome annotation databases

EnsemblPlantsiAT5G46180.1; AT5G46180.1; AT5G46180.
GeneIDi834660.
GrameneiAT5G46180.1; AT5G46180.1; AT5G46180.
KEGGiath:AT5G46180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006698 Genomic DNA. Translation: BAB08263.1.
CP002688 Genomic DNA. Translation: AED95350.1.
BT023421 mRNA. Translation: AAY56412.1.
BT029160 mRNA. Translation: ABJ17095.1.
RefSeqiNP_199430.1. NM_123987.3.
UniGeneiAt.28104.

3D structure databases

ProteinModelPortaliQ9FNK4.
SMRiQ9FNK4. Positions 37-443.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G46180.1.

PTM databases

iPTMnetiQ9FNK4.
SwissPalmiQ9FNK4.

Proteomic databases

PaxDbiQ9FNK4.
PRIDEiQ9FNK4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G46180.1; AT5G46180.1; AT5G46180.
GeneIDi834660.
GrameneiAT5G46180.1; AT5G46180.1; AT5G46180.
KEGGiath:AT5G46180.

Organism-specific databases

TAIRiAT5G46180.

Phylogenomic databases

eggNOGiKOG1402. Eukaryota.
COG4992. LUCA.
HOGENOMiHOG000020206.
InParanoidiQ9FNK4.
KOiK00819.
OMAiTHDHTIR.
OrthoDBiEOG093609WJ.
PhylomeDBiQ9FNK4.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00358.
BioCyciARA:AT5G46180-MONOMER.
MetaCyc:AT5G46180-MONOMER.
BRENDAi2.6.1.13. 399.
ReactomeiR-ATH-70614. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

PROiQ9FNK4.

Gene expression databases

GenevisibleiQ9FNK4. AT.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR005814. Aminotrans_3.
IPR010164. Orn_aminotrans.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PTHR11986:SF18. PTHR11986:SF18. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01885. Orn_aminotrans. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOAT_ARATH
AccessioniPrimary (citable) accession number: Q9FNK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.