Q9FNJ8 (AROD5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arogenate dehydratase 5, chloroplastic
Short name=AtADT5
EC=4.2.1.91

Gene names

Name:	ADT5
Ordered Locus Names:	At5g22630
ORF Names:	MDJ22.5

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts the prephenate produced from the shikimate-chorismate pathway into phenylalanine. Ref.6
Catalytic activity	L-arogenate = L-phenylalanine + H2O + CO2.
Pathway	Amino-acid biosynthesis; L-phenylalanine biosynthesis; L-phenylalanine from L-arogenate: step 1/1.
Subcellular location	Plastid › chloroplast stroma Ref.7.
Tissue specificity	Expressed in roots, leaves, stems, flowers and siliques. More abundant in stems and roots. Ref.6
Induction	Strongly up-regulated during stem elongation. Ref.5
Miscellaneous	Has no detectable prehenate dehydratase activity.
Sequence similarities	Contains 1 prephenate dehydratase domain.
Biophysicochemical properties	Kinetic parameters: KM=1.72 mM for arogenate Ref.6 Vmax=11.26 pmol/sec/µg enzyme with arogenate as substrate

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Phenylalanine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-phenylalanine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast Inferred from direct assay Ref.7. Source: TAIR chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	arogenate dehydratase activity Inferred from direct assay Ref.6. Source: TAIR prephenate dehydratase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 38	38	Chloroplast Potential
	Chain	39 – 425	387	Arogenate dehydratase 5, chloroplastic		PRO_0000373794
Regions
	Domain	127 – 304	178	Prephenate dehydratase
	Compositional bias	36 – 99	64	Ser-rich
	Compositional bias	245 – 277	33	Ala-rich

Sequences

	Sequence		Length	Mass (Da)
	Q9FNJ8 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 7B6AD16F3DE48B3F	FASTA	425	45,928
	10 20 30 40 50 60 MQTISPAFSC DLKSVIQPNL TAKKARYSHV NGKRVSVRCS YRSESFSFPN GVGSSRADWQ 70 80 90 100 110 120 SSCAILASKV VSAENSSSVA VVNGHSNGSV DLSLVPSKSQ HNGKPGLIQP LTITDLSPAP 130 140 150 160 170 180 SHGSTLRVAY QGVPGAYSEA AAGKAYPNSE AIPCDQFDVA FQAVELWIAD RAVLPVENSL 190 200 210 220 230 240 GGSIHRNYDL LLRHRLHIVG EVQIPVHHCL LALPGVRTDC ITRVISHPQA LAQTEGSLNK 250 260 270 280 290 300 LTPKAAIEAF HDTAAAAEYI AANNLHDTAA VASARAAELY GLQILADGIQ DDAGNVTRFL 310 320 330 340 350 360 MLARDPIIPR TDRPFKTSIV FAAQEHKGTS VLFKVLSAFA FRNISLTKIE SRPHQNCPVR 370 380 390 400 410 420 VVGDENVGTS KHFEYTFYVD FEASMAEARA QNALAEVQEY TSFLRVLGSY PMDMTPWSTL PSEDV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Matringe M., Grisollet D., Rippert P. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones." Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:291-300(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Global transcript profiling of primary stems from Arabidopsis thaliana identifies candidate genes for missing links in lignin biosynthesis and transcriptional regulators of fiber differentiation." Ehlting J., Mattheus N., Aeschliman D.S., Li E., Hamberger B., Cullis I.F., Zhuang J., Kaneda M., Mansfield S.D., Samuels L., Ritland K., Ellis B.E., Bohlmann J., Douglas C.J. Plant J. 42:618-640(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[6]	"Phenylalanine biosynthesis in Arabidopsis thaliana. Identification and characterization of arogenate dehydratases." Cho M.-H., Corea O.R.A., Yang H., Bedgar D.L., Laskar D.D., Anterola A.M., Moog-Anterola F.A., Hood R.L., Kohalmi S.E., Bernards M.A., Kang C., Davin L.B., Lewis N.G. J. Biol. Chem. 282:30827-30835(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES.
[7]	"Tyrosine and phenylalanine are synthesized within the plastids in Arabidopsis." Rippert P., Puyaubert J., Grisollet D., Derrier L., Matringe M. Plant Physiol. 149:1251-1260(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ411469 mRNA. Translation: ABD67755.1. AB006699 Genomic DNA. Translation: BAB11669.1. CP002688 Genomic DNA. Translation: AED93055.1. AY058097 mRNA. Translation: AAL24205.1. AY090235 mRNA. Translation: AAL90899.1. AY149958 mRNA. Translation: AAN31112.1.
IPI	IPI00528682.
RefSeq	NP_197655.1. NM_122169.2.
UniGene	At.20326. At.71496.
3D structure databases
ProteinModelPortal	Q9FNJ8.
SMR	Q9FNJ8. Positions 128-410.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9FNJ8. 5 interactions.
STRING	3702.AT5G22630.1-P.
Proteomic databases
PaxDb	Q9FNJ8.
PRIDE	Q9FNJ8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G22630.1; AT5G22630.1; AT5G22630.
GeneID	832326.
KEGG	ath:AT5G22630.
Organism-specific databases
TAIR	At5g22630.
Phylogenomic databases
eggNOG	COG0077.
HOGENOM	HOG000018970.
InParanoid	Q9FNJ8.
KO	K05359.
OMA	CRKWLDA.
PhylomeDB	Q9FNJ8.
ProtClustDB	PLN02317.
Enzyme and pathway databases
BRENDA	4.2.1.91. 399.
SABIO-RK	Q9FNJ8.
UniPathway	UPA00121; UER00344.
Gene expression databases
Genevestigator	Q9FNJ8.
Family and domain databases
InterPro	IPR001086. Preph_deHydtase. IPR018528. Preph_deHydtase_CS. [Graphical view]
Pfam	PF00800. PDT. 1 hit. [Graphical view]
PROSITE	PS00857. PREPHENATE_DEHYDR_1. 1 hit. PS00858. PREPHENATE_DEHYDR_2. 1 hit. PS51171. PREPHENATE_DEHYDR_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROD5_ARATH

Accession

Primary (citable) accession number: Q9FNJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program