Q9FND9 (RFS5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable galactinol--sucrose galactosyltransferase 5
EC=2.4.1.82

Alternative name(s):
Protein SEED IMBIBITION 1-LIKE
Raffinose synthase 5

Gene names

Name:	RFS5
Synonyms:	RS5
Ordered Locus Names:	At5g40390
ORF Names:	MPO12.100, MPO12.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	783 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers By similarity.
Catalytic activity	Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.
Induction	By oxidative stress and cold treatment. Ref.6 Ref.7
Disruption phenotype	Reduced drought tolerance, but no effect on freezing tolerance or on the ability to cold acclimation. Ref.4 Ref.5
Sequence similarities	Belongs to the glycosyl hydrolases 36 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	galactose metabolic process Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to abscisic acid stimulus Inferred from direct assay PubMed 20733066. Source: TAIR response to oxidative stress Inferred from expression pattern Ref.7. Source: TAIR response to water deprivation Inferred from expression pattern PubMed 19502356. Source: TAIR
Cellular_component	chloroplast Inferred from direct assay PubMed 18431481. Source: TAIR
Molecular_function	galactinol-sucrose galactosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 783	783	Probable galactinol--sucrose galactosyltransferase 5		PRO_0000389258
Amino acid modifications
	Modified residue	9	1	Phosphoserine Ref.8
	Modified residue	11	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q9FND9 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 3C37D1D7871888AC	FASTA	783	86,237
	10 20 30 40 50 60 MASPCLTKSD SGINGVDFTE KFRLEDSTLL ANGQVVLTDV PVNVTLTSSP YLVDKDGVPL 70 80 90 100 110 120 DVSAGSFIGF NLDGEPKSHH VASIGKLKNI RFMSIFRFKV WWTTHWVGSN GRDIENETQI 130 140 150 160 170 180 IILDQSGSDS GPGSGSGRPY VLLLPLLEGS FRSSFQSGED DDVAVCVESG STEVTGSEFR 190 200 210 220 230 240 QIVYVHAGDD PFKLVKDAMK VIRVHMNTFK LLEEKSPPGI VDKFGWCTWD AFYLTVNPDG 250 260 270 280 290 300 VHKGVKCLVD GGCPPGLVLI DDGWQSIGHD SDGIDVEGMN ITVAGEQMPC RLLKFEENHK 310 320 330 340 350 360 FKDYVSPKDQ NDVGMKAFVR DLKDEFSTVD YIYVWHALCG YWGGLRPEAP ALPPSTIIRP 370 380 390 400 410 420 ELSPGLKLTM EDLAVDKIIE TGIGFASPDL AKEFYEGLHS HLQNAGIDGV KVDVIHILEM 430 440 450 460 470 480 LCQKYGGRVD LAKAYFKALT SSVNKHFNGN GVIASMEHCN DFMFLGTEAI SLGRVGDDFW 490 500 510 520 530 540 CTDPSGDPNG TFWLQGCHMV HCAYNSLWMG NFIQPDWDMF QSTHPCAEFH AASRAISGGP 550 560 570 580 590 600 IYISDCVGKH DFDLLKRLVL PNGSILRCEY YALPTRDRLF EDPLHDGKTM LKIWNLNKYT 610 620 630 640 650 660 GVIGAFNCQG GGWCRETRRN QCFSECVNTL TATTSPKDVE WNSGSSPISI ANVEEFALFL 670 680 690 700 710 720 SQSKKLLLSG LNDDLELTLE PFKFELITVS PVVTIEGNSV RFAPIGLVNM LNTSGAIRSL 730 740 750 760 770 780 VYNDESVEVG VFGAGEFRVY ASKKPVSCLI DGEVVEFGYE DSMVMVQVPW SGPDGLSSIQ YLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones." Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:291-300(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Inactivation of Arabidopsis SIP1 leads to reduced levels of sugars and drought tolerance." Anderson C.M., Kohorn B.D. J. Plant Physiol. 158:1215-1219(2001) Cited for: DISRUPTION PHENOTYPE.
[5]	"The role of raffinose in the cold acclimation response of Arabidopsis thaliana." Zuther E., Buechel K., Hundertmark M., Stitt M., Hincha D.K., Heyer A.G. FEBS Lett. 576:169-173(2004) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[6]	"Transcript and metabolite profiling during cold acclimation of Arabidopsis reveals an intricate relationship of cold-regulated gene expression with modifications in metabolite content." Kaplan F., Kopka J., Sung D.Y., Zhao W., Popp M., Porat R., Guy C.L. Plant J. 50:967-981(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY COLD.
[7]	"Galactinol and raffinose constitute a novel function to protect plants from oxidative damage." Nishizawa A., Yabuta Y., Shigeoka S. Plant Physiol. 147:1251-1263(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, NOMENCLATURE.
[8]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9 AND SER-11, MASS SPECTROMETRY. Strain: cv. Columbia. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB006702 Genomic DNA. Translation: BAB11595.1. CP002688 Genomic DNA. Translation: AED94542.1. AY062781 mRNA. Translation: AAL32859.1. AY081645 mRNA. Translation: AAM10207.1.
IPI	IPI00530152.
RefSeq	NP_198855.1. NM_123403.3.
UniGene	At.8441.
3D structure databases
ProteinModelPortal	Q9FND9.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G40390.1-P.
Protein family/group databases
CAZy	GH36. Glycoside Hydrolase Family 36.
Proteomic databases
PaxDb	Q9FND9.
PRIDE	Q9FND9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G40390.1; AT5G40390.1; AT5G40390.
GeneID	834037.
KEGG	ath:AT5G40390.
Organism-specific databases
TAIR	At5g40390.
Phylogenomic databases
eggNOG	NOG287560.
HOGENOM	HOG000237551.
InParanoid	Q9FND9.
KO	K06617.
OMA	ETRRNQC.
PhylomeDB	Q9FND9.
ProtClustDB	PLN02711.
Family and domain databases
Gene3D	3.20.20.70. 2 hits.
InterPro	IPR013785. Aldolase_TIM. IPR017853. Glycoside_hydrolase_SF. IPR008811. Raffinose_synthase. [Graphical view]
Pfam	PF05691. Raffinose_syn. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RFS5_ARATH

Accession

Primary (citable) accession number: Q9FND9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 24, 2009
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program