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Q9FND9

- RFS5_ARATH

UniProt

Q9FND9 - RFS5_ARATH

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Protein

Probable galactinol--sucrose galactosyltransferase 5

Gene

RFS5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers (By similarity).By similarity

Catalytic activityi

Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.

GO - Molecular functioni

  1. galactinol-sucrose galactosyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
  2. response to abscisic acid Source: TAIR
  3. response to oxidative stress Source: TAIR
  4. response to water deprivation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Protein family/group databases

CAZyiGH36. Glycoside Hydrolase Family 36.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable galactinol--sucrose galactosyltransferase 5 (EC:2.4.1.82)
Alternative name(s):
Protein SEED IMBIBITION 1-LIKE
Raffinose synthase 5
Gene namesi
Name:RFS5
Synonyms:RS5
Ordered Locus Names:At5g40390
ORF Names:MPO12.100, MPO12.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G40390.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Reduced drought tolerance, but no effect on freezing tolerance or on the ability to cold acclimation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 783783Probable galactinol--sucrose galactosyltransferase 5PRO_0000389258Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei9 – 91Phosphoserine1 Publication
Modified residuei11 – 111Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9FND9.
PRIDEiQ9FND9.

Expressioni

Inductioni

By oxidative stress and cold treatment.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi19288. 1 interaction.
STRINGi3702.AT5G40390.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9FND9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolases 36 family.Curated

Phylogenomic databases

eggNOGiNOG287560.
HOGENOMiHOG000237551.
InParanoidiQ9FND9.
KOiK06617.
OMAiSTHPCAA.
PhylomeDBiQ9FND9.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR008811. Glycosyl_hydrolases_36.
[Graphical view]
PfamiPF05691. Raffinose_syn. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 3 hits.

Sequencei

Sequence statusi: Complete.

Q9FND9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASPCLTKSD SGINGVDFTE KFRLEDSTLL ANGQVVLTDV PVNVTLTSSP
60 70 80 90 100
YLVDKDGVPL DVSAGSFIGF NLDGEPKSHH VASIGKLKNI RFMSIFRFKV
110 120 130 140 150
WWTTHWVGSN GRDIENETQI IILDQSGSDS GPGSGSGRPY VLLLPLLEGS
160 170 180 190 200
FRSSFQSGED DDVAVCVESG STEVTGSEFR QIVYVHAGDD PFKLVKDAMK
210 220 230 240 250
VIRVHMNTFK LLEEKSPPGI VDKFGWCTWD AFYLTVNPDG VHKGVKCLVD
260 270 280 290 300
GGCPPGLVLI DDGWQSIGHD SDGIDVEGMN ITVAGEQMPC RLLKFEENHK
310 320 330 340 350
FKDYVSPKDQ NDVGMKAFVR DLKDEFSTVD YIYVWHALCG YWGGLRPEAP
360 370 380 390 400
ALPPSTIIRP ELSPGLKLTM EDLAVDKIIE TGIGFASPDL AKEFYEGLHS
410 420 430 440 450
HLQNAGIDGV KVDVIHILEM LCQKYGGRVD LAKAYFKALT SSVNKHFNGN
460 470 480 490 500
GVIASMEHCN DFMFLGTEAI SLGRVGDDFW CTDPSGDPNG TFWLQGCHMV
510 520 530 540 550
HCAYNSLWMG NFIQPDWDMF QSTHPCAEFH AASRAISGGP IYISDCVGKH
560 570 580 590 600
DFDLLKRLVL PNGSILRCEY YALPTRDRLF EDPLHDGKTM LKIWNLNKYT
610 620 630 640 650
GVIGAFNCQG GGWCRETRRN QCFSECVNTL TATTSPKDVE WNSGSSPISI
660 670 680 690 700
ANVEEFALFL SQSKKLLLSG LNDDLELTLE PFKFELITVS PVVTIEGNSV
710 720 730 740 750
RFAPIGLVNM LNTSGAIRSL VYNDESVEVG VFGAGEFRVY ASKKPVSCLI
760 770 780
DGEVVEFGYE DSMVMVQVPW SGPDGLSSIQ YLF
Length:783
Mass (Da):86,237
Last modified:March 1, 2001 - v1
Checksum:i3C37D1D7871888AC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB006702 Genomic DNA. Translation: BAB11595.1.
CP002688 Genomic DNA. Translation: AED94542.1.
AY062781 mRNA. Translation: AAL32859.1.
AY081645 mRNA. Translation: AAM10207.1.
RefSeqiNP_198855.1. NM_123403.3.
UniGeneiAt.8441.

Genome annotation databases

EnsemblPlantsiAT5G40390.1; AT5G40390.1; AT5G40390.
GeneIDi834037.
KEGGiath:AT5G40390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB006702 Genomic DNA. Translation: BAB11595.1 .
CP002688 Genomic DNA. Translation: AED94542.1 .
AY062781 mRNA. Translation: AAL32859.1 .
AY081645 mRNA. Translation: AAM10207.1 .
RefSeqi NP_198855.1. NM_123403.3.
UniGenei At.8441.

3D structure databases

ProteinModelPortali Q9FND9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 19288. 1 interaction.
STRINGi 3702.AT5G40390.1-P.

Protein family/group databases

CAZyi GH36. Glycoside Hydrolase Family 36.

Proteomic databases

PaxDbi Q9FND9.
PRIDEi Q9FND9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G40390.1 ; AT5G40390.1 ; AT5G40390 .
GeneIDi 834037.
KEGGi ath:AT5G40390.

Organism-specific databases

TAIRi AT5G40390.

Phylogenomic databases

eggNOGi NOG287560.
HOGENOMi HOG000237551.
InParanoidi Q9FND9.
KOi K06617.
OMAi STHPCAA.
PhylomeDBi Q9FND9.

Family and domain databases

Gene3Di 3.20.20.70. 2 hits.
InterProi IPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR008811. Glycosyl_hydrolases_36.
[Graphical view ]
Pfami PF05691. Raffinose_syn. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones."
    Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 4:291-300(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Inactivation of Arabidopsis SIP1 leads to reduced levels of sugars and drought tolerance."
    Anderson C.M., Kohorn B.D.
    J. Plant Physiol. 158:1215-1219(2001)
    Cited for: DISRUPTION PHENOTYPE.
  5. "The role of raffinose in the cold acclimation response of Arabidopsis thaliana."
    Zuther E., Buechel K., Hundertmark M., Stitt M., Hincha D.K., Heyer A.G.
    FEBS Lett. 576:169-173(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  6. "Transcript and metabolite profiling during cold acclimation of Arabidopsis reveals an intricate relationship of cold-regulated gene expression with modifications in metabolite content."
    Kaplan F., Kopka J., Sung D.Y., Zhao W., Popp M., Porat R., Guy C.L.
    Plant J. 50:967-981(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY COLD.
  7. "Galactinol and raffinose constitute a novel function to protect plants from oxidative damage."
    Nishizawa A., Yabuta Y., Shigeoka S.
    Plant Physiol. 147:1251-1263(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, NOMENCLATURE.
  8. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
  9. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9 AND SER-11, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRFS5_ARATH
AccessioniPrimary (citable) accession number: Q9FND9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: March 1, 2001
Last modified: October 1, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3