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Q9FND9

- RFS5_ARATH

UniProt

Q9FND9 - RFS5_ARATH

Protein

Probable galactinol--sucrose galactosyltransferase 5

Gene

RFS5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers By similarity.By similarity

    Catalytic activityi

    Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.

    GO - Molecular functioni

    1. galactinol-sucrose galactosyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW
    2. response to abscisic acid Source: TAIR
    3. response to oxidative stress Source: TAIR
    4. response to water deprivation Source: TAIR

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism

    Protein family/group databases

    CAZyiGH36. Glycoside Hydrolase Family 36.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable galactinol--sucrose galactosyltransferase 5 (EC:2.4.1.82)
    Alternative name(s):
    Protein SEED IMBIBITION 1-LIKE
    Raffinose synthase 5
    Gene namesi
    Name:RFS5
    Synonyms:RS5
    Ordered Locus Names:At5g40390
    ORF Names:MPO12.100, MPO12.13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G40390.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR

    Pathology & Biotechi

    Disruption phenotypei

    Reduced drought tolerance, but no effect on freezing tolerance or on the ability to cold acclimation.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 783783Probable galactinol--sucrose galactosyltransferase 5PRO_0000389258Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei9 – 91Phosphoserine1 Publication
    Modified residuei11 – 111Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9FND9.
    PRIDEiQ9FND9.

    Expressioni

    Inductioni

    By oxidative stress and cold treatment.2 Publications

    Interactioni

    Protein-protein interaction databases

    BioGridi19288. 1 interaction.
    STRINGi3702.AT5G40390.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FND9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolases 36 family.Curated

    Phylogenomic databases

    eggNOGiNOG287560.
    HOGENOMiHOG000237551.
    InParanoidiQ9FND9.
    KOiK06617.
    OMAiSTHPCAA.
    PhylomeDBiQ9FND9.

    Family and domain databases

    Gene3Di3.20.20.70. 2 hits.
    InterProiIPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR008811. Glycosyl_hydrolases_36.
    [Graphical view]
    PfamiPF05691. Raffinose_syn. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    Q9FND9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASPCLTKSD SGINGVDFTE KFRLEDSTLL ANGQVVLTDV PVNVTLTSSP    50
    YLVDKDGVPL DVSAGSFIGF NLDGEPKSHH VASIGKLKNI RFMSIFRFKV 100
    WWTTHWVGSN GRDIENETQI IILDQSGSDS GPGSGSGRPY VLLLPLLEGS 150
    FRSSFQSGED DDVAVCVESG STEVTGSEFR QIVYVHAGDD PFKLVKDAMK 200
    VIRVHMNTFK LLEEKSPPGI VDKFGWCTWD AFYLTVNPDG VHKGVKCLVD 250
    GGCPPGLVLI DDGWQSIGHD SDGIDVEGMN ITVAGEQMPC RLLKFEENHK 300
    FKDYVSPKDQ NDVGMKAFVR DLKDEFSTVD YIYVWHALCG YWGGLRPEAP 350
    ALPPSTIIRP ELSPGLKLTM EDLAVDKIIE TGIGFASPDL AKEFYEGLHS 400
    HLQNAGIDGV KVDVIHILEM LCQKYGGRVD LAKAYFKALT SSVNKHFNGN 450
    GVIASMEHCN DFMFLGTEAI SLGRVGDDFW CTDPSGDPNG TFWLQGCHMV 500
    HCAYNSLWMG NFIQPDWDMF QSTHPCAEFH AASRAISGGP IYISDCVGKH 550
    DFDLLKRLVL PNGSILRCEY YALPTRDRLF EDPLHDGKTM LKIWNLNKYT 600
    GVIGAFNCQG GGWCRETRRN QCFSECVNTL TATTSPKDVE WNSGSSPISI 650
    ANVEEFALFL SQSKKLLLSG LNDDLELTLE PFKFELITVS PVVTIEGNSV 700
    RFAPIGLVNM LNTSGAIRSL VYNDESVEVG VFGAGEFRVY ASKKPVSCLI 750
    DGEVVEFGYE DSMVMVQVPW SGPDGLSSIQ YLF 783
    Length:783
    Mass (Da):86,237
    Last modified:March 1, 2001 - v1
    Checksum:i3C37D1D7871888AC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB006702 Genomic DNA. Translation: BAB11595.1.
    CP002688 Genomic DNA. Translation: AED94542.1.
    AY062781 mRNA. Translation: AAL32859.1.
    AY081645 mRNA. Translation: AAM10207.1.
    RefSeqiNP_198855.1. NM_123403.3.
    UniGeneiAt.8441.

    Genome annotation databases

    EnsemblPlantsiAT5G40390.1; AT5G40390.1; AT5G40390.
    GeneIDi834037.
    KEGGiath:AT5G40390.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB006702 Genomic DNA. Translation: BAB11595.1 .
    CP002688 Genomic DNA. Translation: AED94542.1 .
    AY062781 mRNA. Translation: AAL32859.1 .
    AY081645 mRNA. Translation: AAM10207.1 .
    RefSeqi NP_198855.1. NM_123403.3.
    UniGenei At.8441.

    3D structure databases

    ProteinModelPortali Q9FND9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 19288. 1 interaction.
    STRINGi 3702.AT5G40390.1-P.

    Protein family/group databases

    CAZyi GH36. Glycoside Hydrolase Family 36.

    Proteomic databases

    PaxDbi Q9FND9.
    PRIDEi Q9FND9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G40390.1 ; AT5G40390.1 ; AT5G40390 .
    GeneIDi 834037.
    KEGGi ath:AT5G40390.

    Organism-specific databases

    TAIRi AT5G40390.

    Phylogenomic databases

    eggNOGi NOG287560.
    HOGENOMi HOG000237551.
    InParanoidi Q9FND9.
    KOi K06617.
    OMAi STHPCAA.
    PhylomeDBi Q9FND9.

    Family and domain databases

    Gene3Di 3.20.20.70. 2 hits.
    InterProi IPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR008811. Glycosyl_hydrolases_36.
    [Graphical view ]
    Pfami PF05691. Raffinose_syn. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones."
      Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 4:291-300(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Inactivation of Arabidopsis SIP1 leads to reduced levels of sugars and drought tolerance."
      Anderson C.M., Kohorn B.D.
      J. Plant Physiol. 158:1215-1219(2001)
      Cited for: DISRUPTION PHENOTYPE.
    5. "The role of raffinose in the cold acclimation response of Arabidopsis thaliana."
      Zuther E., Buechel K., Hundertmark M., Stitt M., Hincha D.K., Heyer A.G.
      FEBS Lett. 576:169-173(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    6. "Transcript and metabolite profiling during cold acclimation of Arabidopsis reveals an intricate relationship of cold-regulated gene expression with modifications in metabolite content."
      Kaplan F., Kopka J., Sung D.Y., Zhao W., Popp M., Porat R., Guy C.L.
      Plant J. 50:967-981(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY COLD.
    7. "Galactinol and raffinose constitute a novel function to protect plants from oxidative damage."
      Nishizawa A., Yabuta Y., Shigeoka S.
      Plant Physiol. 147:1251-1263(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, NOMENCLATURE.
    8. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
      Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
      J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.
    9. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
      Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
      Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9 AND SER-11, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRFS5_ARATH
    AccessioniPrimary (citable) accession number: Q9FND9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3