Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9FND9 (RFS5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable galactinol--sucrose galactosyltransferase 5

EC=2.4.1.82
Alternative name(s):
Protein SEED IMBIBITION 1-LIKE
Raffinose synthase 5
Gene names
Name:RFS5
Synonyms:RS5
Ordered Locus Names:At5g40390
ORF Names:MPO12.100, MPO12.13
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length783 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers By similarity.

Catalytic activity

Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.

Induction

By oxidative stress and cold treatment. Ref.6 Ref.7

Disruption phenotype

Reduced drought tolerance, but no effect on freezing tolerance or on the ability to cold acclimation. Ref.4 Ref.5

Sequence similarities

Belongs to the glycosyl hydrolases 36 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 783783Probable galactinol--sucrose galactosyltransferase 5
PRO_0000389258

Amino acid modifications

Modified residue91Phosphoserine Ref.9
Modified residue111Phosphoserine Ref.9

Sequences

Sequence LengthMass (Da)Tools
Q9FND9 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 3C37D1D7871888AC

FASTA78386,237
        10         20         30         40         50         60 
MASPCLTKSD SGINGVDFTE KFRLEDSTLL ANGQVVLTDV PVNVTLTSSP YLVDKDGVPL 

        70         80         90        100        110        120 
DVSAGSFIGF NLDGEPKSHH VASIGKLKNI RFMSIFRFKV WWTTHWVGSN GRDIENETQI 

       130        140        150        160        170        180 
IILDQSGSDS GPGSGSGRPY VLLLPLLEGS FRSSFQSGED DDVAVCVESG STEVTGSEFR 

       190        200        210        220        230        240 
QIVYVHAGDD PFKLVKDAMK VIRVHMNTFK LLEEKSPPGI VDKFGWCTWD AFYLTVNPDG 

       250        260        270        280        290        300 
VHKGVKCLVD GGCPPGLVLI DDGWQSIGHD SDGIDVEGMN ITVAGEQMPC RLLKFEENHK 

       310        320        330        340        350        360 
FKDYVSPKDQ NDVGMKAFVR DLKDEFSTVD YIYVWHALCG YWGGLRPEAP ALPPSTIIRP 

       370        380        390        400        410        420 
ELSPGLKLTM EDLAVDKIIE TGIGFASPDL AKEFYEGLHS HLQNAGIDGV KVDVIHILEM 

       430        440        450        460        470        480 
LCQKYGGRVD LAKAYFKALT SSVNKHFNGN GVIASMEHCN DFMFLGTEAI SLGRVGDDFW 

       490        500        510        520        530        540 
CTDPSGDPNG TFWLQGCHMV HCAYNSLWMG NFIQPDWDMF QSTHPCAEFH AASRAISGGP 

       550        560        570        580        590        600 
IYISDCVGKH DFDLLKRLVL PNGSILRCEY YALPTRDRLF EDPLHDGKTM LKIWNLNKYT 

       610        620        630        640        650        660 
GVIGAFNCQG GGWCRETRRN QCFSECVNTL TATTSPKDVE WNSGSSPISI ANVEEFALFL 

       670        680        690        700        710        720 
SQSKKLLLSG LNDDLELTLE PFKFELITVS PVVTIEGNSV RFAPIGLVNM LNTSGAIRSL 

       730        740        750        760        770        780 
VYNDESVEVG VFGAGEFRVY ASKKPVSCLI DGEVVEFGYE DSMVMVQVPW SGPDGLSSIQ 


YLF 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones."
Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S.
DNA Res. 4:291-300(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Inactivation of Arabidopsis SIP1 leads to reduced levels of sugars and drought tolerance."
Anderson C.M., Kohorn B.D.
J. Plant Physiol. 158:1215-1219(2001)
Cited for: DISRUPTION PHENOTYPE.
[5]"The role of raffinose in the cold acclimation response of Arabidopsis thaliana."
Zuther E., Buechel K., Hundertmark M., Stitt M., Hincha D.K., Heyer A.G.
FEBS Lett. 576:169-173(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[6]"Transcript and metabolite profiling during cold acclimation of Arabidopsis reveals an intricate relationship of cold-regulated gene expression with modifications in metabolite content."
Kaplan F., Kopka J., Sung D.Y., Zhao W., Popp M., Porat R., Guy C.L.
Plant J. 50:967-981(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY COLD.
[7]"Galactinol and raffinose constitute a novel function to protect plants from oxidative damage."
Nishizawa A., Yabuta Y., Shigeoka S.
Plant Physiol. 147:1251-1263(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, NOMENCLATURE.
[8]"Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: cv. Columbia.
[9]"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9 AND SER-11, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB006702 Genomic DNA. Translation: BAB11595.1.
CP002688 Genomic DNA. Translation: AED94542.1.
AY062781 mRNA. Translation: AAL32859.1.
AY081645 mRNA. Translation: AAM10207.1.
RefSeqNP_198855.1. NM_123403.3.
UniGeneAt.8441.

3D structure databases

ProteinModelPortalQ9FND9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid19288. 1 interaction.
STRING3702.AT5G40390.1-P.

Protein family/group databases

CAZyGH36. Glycoside Hydrolase Family 36.

Proteomic databases

PaxDbQ9FND9.
PRIDEQ9FND9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G40390.1; AT5G40390.1; AT5G40390.
GeneID834037.
KEGGath:AT5G40390.

Organism-specific databases

TAIRAT5G40390.

Phylogenomic databases

eggNOGNOG287560.
HOGENOMHOG000237551.
InParanoidQ9FND9.
KOK06617.
OMALIKFQEN.
PhylomeDBQ9FND9.
ProtClustDBPLN02711.

Family and domain databases

Gene3D3.20.20.70. 2 hits.
InterProIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR008811. Glycosyl_hydrolases_36.
[Graphical view]
PfamPF05691. Raffinose_syn. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 3 hits.
ProtoNetSearch...

Entry information

Entry nameRFS5_ARATH
AccessionPrimary (citable) accession number: Q9FND9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: March 1, 2001
Last modified: March 19, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names