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Protein

Probable galactinol--sucrose galactosyltransferase 5

Gene

RFS5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers (By similarity).By similarity

Catalytic activityi

Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: UniProtKB-KW
  • response to abscisic acid Source: TAIR
  • response to oxidative stress Source: TAIR
  • response to water deprivation Source: TAIR

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCarbohydrate metabolism

Enzyme and pathway databases

BioCyciARA:AT5G40390-MONOMER
BRENDAi2.4.1.82 399

Protein family/group databases

CAZyiGH36 Glycoside Hydrolase Family 36

Names & Taxonomyi

Protein namesi
Recommended name:
Probable galactinol--sucrose galactosyltransferase 5 (EC:2.4.1.82)
Alternative name(s):
Protein SEED IMBIBITION 1-LIKE
Raffinose synthase 5
Gene namesi
Name:RFS5
Synonyms:RS5
Ordered Locus Names:At5g40390
ORF Names:MPO12.100, MPO12.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G40390
TAIRilocus:2170528 AT5G40390

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Reduced drought tolerance, but no effect on freezing tolerance or on the ability to cold acclimation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003892581 – 783Probable galactinol--sucrose galactosyltransferase 5Add BLAST783

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineCombined sources1
Modified residuei11PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9FND9
PRIDEiQ9FND9

PTM databases

iPTMnetiQ9FND9

Expressioni

Inductioni

By oxidative stress and cold treatment.2 Publications

Gene expression databases

ExpressionAtlasiQ9FND9 baseline and differential
GenevisibleiQ9FND9 AT

Interactioni

Protein-protein interaction databases

BioGridi19288, 1 interactor
STRINGi3702.AT5G40390.1

Structurei

3D structure databases

ProteinModelPortaliQ9FND9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolases 36 family.Curated

Phylogenomic databases

eggNOGiENOG410IHR2 Eukaryota
ENOG410YBUA LUCA
HOGENOMiHOG000237551
InParanoidiQ9FND9
KOiK06617
OMAiSIGHDSD
OrthoDBiEOG09360287
PhylomeDBiQ9FND9

Family and domain databases

InterProiView protein in InterPro
IPR017853 Glycoside_hydrolase_SF
IPR008811 Glycosyl_hydrolases_36
PANTHERiPTHR31268 PTHR31268, 1 hit
PfamiView protein in Pfam
PF05691 Raffinose_syn, 1 hit
SUPFAMiSSF51445 SSF51445, 3 hits

Sequencei

Sequence statusi: Complete.

Q9FND9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPCLTKSD SGINGVDFTE KFRLEDSTLL ANGQVVLTDV PVNVTLTSSP
60 70 80 90 100
YLVDKDGVPL DVSAGSFIGF NLDGEPKSHH VASIGKLKNI RFMSIFRFKV
110 120 130 140 150
WWTTHWVGSN GRDIENETQI IILDQSGSDS GPGSGSGRPY VLLLPLLEGS
160 170 180 190 200
FRSSFQSGED DDVAVCVESG STEVTGSEFR QIVYVHAGDD PFKLVKDAMK
210 220 230 240 250
VIRVHMNTFK LLEEKSPPGI VDKFGWCTWD AFYLTVNPDG VHKGVKCLVD
260 270 280 290 300
GGCPPGLVLI DDGWQSIGHD SDGIDVEGMN ITVAGEQMPC RLLKFEENHK
310 320 330 340 350
FKDYVSPKDQ NDVGMKAFVR DLKDEFSTVD YIYVWHALCG YWGGLRPEAP
360 370 380 390 400
ALPPSTIIRP ELSPGLKLTM EDLAVDKIIE TGIGFASPDL AKEFYEGLHS
410 420 430 440 450
HLQNAGIDGV KVDVIHILEM LCQKYGGRVD LAKAYFKALT SSVNKHFNGN
460 470 480 490 500
GVIASMEHCN DFMFLGTEAI SLGRVGDDFW CTDPSGDPNG TFWLQGCHMV
510 520 530 540 550
HCAYNSLWMG NFIQPDWDMF QSTHPCAEFH AASRAISGGP IYISDCVGKH
560 570 580 590 600
DFDLLKRLVL PNGSILRCEY YALPTRDRLF EDPLHDGKTM LKIWNLNKYT
610 620 630 640 650
GVIGAFNCQG GGWCRETRRN QCFSECVNTL TATTSPKDVE WNSGSSPISI
660 670 680 690 700
ANVEEFALFL SQSKKLLLSG LNDDLELTLE PFKFELITVS PVVTIEGNSV
710 720 730 740 750
RFAPIGLVNM LNTSGAIRSL VYNDESVEVG VFGAGEFRVY ASKKPVSCLI
760 770 780
DGEVVEFGYE DSMVMVQVPW SGPDGLSSIQ YLF
Length:783
Mass (Da):86,237
Last modified:March 1, 2001 - v1
Checksum:i3C37D1D7871888AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006702 Genomic DNA Translation: BAB11595.1
CP002688 Genomic DNA Translation: AED94542.1
AY062781 mRNA Translation: AAL32859.1
AY081645 mRNA Translation: AAM10207.1
RefSeqiNP_198855.1, NM_123403.4
UniGeneiAt.8441

Genome annotation databases

EnsemblPlantsiAT5G40390.1; AT5G40390.1; AT5G40390
GeneIDi834037
GrameneiAT5G40390.1; AT5G40390.1; AT5G40390
KEGGiath:AT5G40390

Similar proteinsi

Entry informationi

Entry nameiRFS5_ARATH
AccessioniPrimary (citable) accession number: Q9FND9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health