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Protein

Polyamine oxidase 1

Gene

PAO1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Flavoenzyme that catalyzes the oxidation of the secondary amino group of spermine, norspermine and N1-acetylspermine. Substrate preference is norspermine > spermine > N1-acetylspermine. No activity detected when putrescine, spermidine or N1-acetylspermidine are used as substrates. Plays an important role in the regulation of polyamine intracellular concentration.1 Publication

Catalytic activityi

Spermine + O2 + H2O = spermidine + 3-aminopropanal + H2O2.
Spermidine + O2 + H2O = putrescine + 3-aminopropanal + H2O2.
N1-acetylspermine + O2 + H2O = spermidine + 3-acetamidopropanal + H2O2.
Norspermine + O2 + H2O = norspermidine + 3-aminopropanal + H2O2.

Cofactori

FADNote: Binds 1 FAD per subunit.

Enzyme regulationi

Inhibited by guazatine, N-prenylagmatine and 1,12-diaminododecane.

Kineticsi

  1. KM=0.11 mM for spermine1 Publication
  2. KM=0.09 mM for norspermine1 Publication
  3. KM=0.2 mM for N1-acetylspermine1 Publication

    pH dependencei

    Optimum pH is 8.0.1 Publication

    Pathwayi: spermine degradation

    This protein is involved in the pathway spermine degradation, which is part of Amine and polyamine degradation.
    View all proteins of this organism that are known to be involved in the pathway spermine degradation and in Amine and polyamine degradation.

    GO - Molecular functioni

    GO - Biological processi

    • polyamine catabolic process Source: TAIR
    • spermine catabolic process Source: UniProtKB-UniPathway

    Keywordsi

    Molecular functionOxidoreductase
    LigandFAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciARA:AT5G13700-MONOMER
    MetaCyc:AT5G13700-MONOMER
    BRENDAi1.5.3.16 399
    UniPathwayiUPA00211

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyamine oxidase 1 (EC:1.5.3.16)
    Short name:
    AtPAO1
    Alternative name(s):
    N(1)-acetylpolyamine oxidase
    Spermine oxidase
    Gene namesi
    Name:PAO1
    Synonyms:PAO
    Ordered Locus Names:At5g13700
    ORF Names:MSH12.17
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    AraportiAT5G13700
    TAIRilocus:2173219 AT5G13700

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003525071 – 472Polyamine oxidase 1Add BLAST472

    Proteomic databases

    PaxDbiQ9FNA2
    PRIDEiQ9FNA2

    Expressioni

    Tissue specificityi

    Expressed at very low levels in leaves, stems and inflorescences.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9FNA2 baseline and differential
    GenevisibleiQ9FNA2 AT

    Interactioni

    Protein-protein interaction databases

    BioGridi16493, 1 interactor
    STRINGi3702.AT5G13700.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FNA2
    SMRiQ9FNA2
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the flavin monoamine oxidase family.Curated

    Phylogenomic databases

    eggNOGiKOG0029 Eukaryota
    ENOG410XSNC LUCA
    HOGENOMiHOG000174927
    InParanoidiQ9FNA2
    KOiK13366
    OMAiMGAQFIN
    OrthoDBiEOG09360ACK
    PhylomeDBiQ9FNA2

    Family and domain databases

    Gene3Di3.50.50.60, 3 hits
    InterProiView protein in InterPro
    IPR002937 Amino_oxidase
    IPR036188 FAD/NAD-bd_sf
    IPR001613 Flavin_amine_oxidase
    PfamiView protein in Pfam
    PF01593 Amino_oxidase, 2 hits
    PRINTSiPR00757 AMINEOXDASEF
    SUPFAMiSSF51905 SSF51905, 2 hits

    Sequencei

    Sequence statusi: Complete.

    Q9FNA2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSTASVIIIG AGISGISAAK VLVENGVEDV LILEATDRIG GRIHKQNFGD
    60 70 80 90 100
    VPVELGAGWI AGVGGKESNP VWELASRFNL RTCFSDYTNA RFNIYDRSGK
    110 120 130 140 150
    IFPTGIASDS YKKAVDSAIL KLKSLEAQCS GQVAEEAPSS PKTPIELAID
    160 170 180 190 200
    FILHDFEMAE VEPISTYVDF GEREFLVADE RGYECLLYKM AEEFLVTSHG
    210 220 230 240 250
    NILDYRLKLN QVVREVQQSR NGVVVKTEDG SVYEANYVIV SASIGVLQSD
    260 270 280 290 300
    LLSFQPLLPR WKTEAIQKCD VMVYTKIFLK FPQCFWPCGP GQEFFIYAHE
    310 320 330 340 350
    QRGYFTFWQH MENAYPGSNI LVVTLTNEQS KRVEAQSDQE TMKEAMSVLR
    360 370 380 390 400
    DMFGATIPYA TDILVPRWWN NRFQRGSYSN YPMISDNQLL QNIKAPVGRI
    410 420 430 440 450
    FFTGEHTSEK FSGYVHGGYL AGIDTSKSLL EEMKQSLLLQ PLLAFTESLT
    460 470
    LTHQKPNNSQ IYTNVKFISG TS
    Length:472
    Mass (Da):52,866
    Last modified:March 1, 2001 - v1
    Checksum:i139925C472DA57C6
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti21V → I in BAC43225 (PubMed:11910074).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB006704 Genomic DNA Translation: BAB08697.1
    CP002688 Genomic DNA Translation: AED91929.1
    AK118627 mRNA Translation: BAC43225.2
    BT026370 mRNA Translation: ABH04477.1
    RefSeqiNP_196874.1, NM_121373.4
    UniGeneiAt.49017
    At.6477

    Genome annotation databases

    EnsemblPlantsiAT5G13700.1; AT5G13700.1; AT5G13700
    GeneIDi831215
    GrameneiAT5G13700.1; AT5G13700.1; AT5G13700
    KEGGiath:AT5G13700

    Similar proteinsi

    Entry informationi

    Entry nameiPAO1_ARATH
    AccessioniPrimary (citable) accession number: Q9FNA2
    Secondary accession number(s): Q7FL79
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 14, 2008
    Last sequence update: March 1, 2001
    Last modified: April 25, 2018
    This is version 106 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health