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Protein

Polyamine oxidase 1

Gene

PAO1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Flavoenzyme that catalyzes the oxidation of the secondary amino group of spermine, norspermine and N(1)-acetylspermine. Substrate preference is norspermine > spermine > N(1)-acetylspermine. No activity detected when putrescine, spermidine or N(1)-acetylspermidine are used as substrates. Plays an important role in the regulation of polyamine intracellular concentration.1 Publication

Catalytic activityi

Spermine + O2 + H2O = spermidine + 3-aminopropanal + H2O2.
Spermidine + O2 + H2O = putrescine + 3-aminopropanal + H2O2.
N(1)-acetylspermine + O2 + H2O = spermidine + 3-acetamidopropanal + H2O2.
Norspermine + O2 + H2O = norspermidine + 3-aminopropanal + H2O2.

Cofactori

FADNote: Binds 1 FAD per subunit.

Enzyme regulationi

Inhibited by guazatine, N-prenylagmatine and 1,12-diaminododecane.

Kineticsi

  1. KM=0.11 mM for spermine1 Publication
  2. KM=0.09 mM for norspermine1 Publication
  3. KM=0.2 mM for N(1)-acetylspermine1 Publication

    pH dependencei

    Optimum pH is 8.0.1 Publication

    Pathwayi: spermine degradation

    This protein is involved in the pathway spermine degradation, which is part of Amine and polyamine degradation.
    View all proteins of this organism that are known to be involved in the pathway spermine degradation and in Amine and polyamine degradation.

    GO - Molecular functioni

    GO - Biological processi

    • polyamine catabolic process Source: TAIR
    • spermine catabolic process Source: UniProtKB-UniPathway
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciARA:AT5G13700-MONOMER.
    MetaCyc:AT5G13700-MONOMER.
    BRENDAi1.5.3.16. 399.
    UniPathwayiUPA00211.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyamine oxidase 1 (EC:1.5.3.16)
    Short name:
    AtPAO1
    Alternative name(s):
    N(1)-acetylpolyamine oxidase
    Spermine oxidase
    Gene namesi
    Name:PAO1
    Synonyms:PAO
    Ordered Locus Names:At5g13700
    ORF Names:MSH12.17
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    TAIRiAT5G13700.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 472472Polyamine oxidase 1PRO_0000352507Add
    BLAST

    Proteomic databases

    PaxDbiQ9FNA2.
    PRIDEiQ9FNA2.

    Expressioni

    Tissue specificityi

    Expressed at very low levels in leaves, stems and inflorescences.1 Publication

    Gene expression databases

    GenevisibleiQ9FNA2. AT.

    Interactioni

    Protein-protein interaction databases

    BioGridi16493. 1 interaction.
    STRINGi3702.AT5G13700.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FNA2.
    SMRiQ9FNA2. Positions 3-430.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the flavin monoamine oxidase family.Curated

    Phylogenomic databases

    eggNOGiKOG0029. Eukaryota.
    ENOG410XSNC. LUCA.
    HOGENOMiHOG000174927.
    InParanoidiQ9FNA2.
    KOiK13366.
    OMAiETCERTK.
    OrthoDBiEOG09360ACK.
    PhylomeDBiQ9FNA2.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002937. Amino_oxidase.
    IPR023753. FAD/NAD-binding_dom.
    IPR001613. Flavin_amine_oxidase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 2 hits.
    [Graphical view]
    PRINTSiPR00757. AMINEOXDASEF.
    SUPFAMiSSF51905. SSF51905. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q9FNA2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSTASVIIIG AGISGISAAK VLVENGVEDV LILEATDRIG GRIHKQNFGD
    60 70 80 90 100
    VPVELGAGWI AGVGGKESNP VWELASRFNL RTCFSDYTNA RFNIYDRSGK
    110 120 130 140 150
    IFPTGIASDS YKKAVDSAIL KLKSLEAQCS GQVAEEAPSS PKTPIELAID
    160 170 180 190 200
    FILHDFEMAE VEPISTYVDF GEREFLVADE RGYECLLYKM AEEFLVTSHG
    210 220 230 240 250
    NILDYRLKLN QVVREVQQSR NGVVVKTEDG SVYEANYVIV SASIGVLQSD
    260 270 280 290 300
    LLSFQPLLPR WKTEAIQKCD VMVYTKIFLK FPQCFWPCGP GQEFFIYAHE
    310 320 330 340 350
    QRGYFTFWQH MENAYPGSNI LVVTLTNEQS KRVEAQSDQE TMKEAMSVLR
    360 370 380 390 400
    DMFGATIPYA TDILVPRWWN NRFQRGSYSN YPMISDNQLL QNIKAPVGRI
    410 420 430 440 450
    FFTGEHTSEK FSGYVHGGYL AGIDTSKSLL EEMKQSLLLQ PLLAFTESLT
    460 470
    LTHQKPNNSQ IYTNVKFISG TS
    Length:472
    Mass (Da):52,866
    Last modified:March 1, 2001 - v1
    Checksum:i139925C472DA57C6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti21 – 211V → I in BAC43225 (PubMed:11910074).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB006704 Genomic DNA. Translation: BAB08697.1.
    CP002688 Genomic DNA. Translation: AED91929.1.
    AK118627 mRNA. Translation: BAC43225.2.
    BT026370 mRNA. Translation: ABH04477.1.
    RefSeqiNP_196874.1. NM_121373.3.
    UniGeneiAt.49017.
    At.6477.

    Genome annotation databases

    EnsemblPlantsiAT5G13700.1; AT5G13700.1; AT5G13700.
    GeneIDi831215.
    GrameneiAT5G13700.1; AT5G13700.1; AT5G13700.
    KEGGiath:AT5G13700.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB006704 Genomic DNA. Translation: BAB08697.1.
    CP002688 Genomic DNA. Translation: AED91929.1.
    AK118627 mRNA. Translation: BAC43225.2.
    BT026370 mRNA. Translation: ABH04477.1.
    RefSeqiNP_196874.1. NM_121373.3.
    UniGeneiAt.49017.
    At.6477.

    3D structure databases

    ProteinModelPortaliQ9FNA2.
    SMRiQ9FNA2. Positions 3-430.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi16493. 1 interaction.
    STRINGi3702.AT5G13700.1.

    Proteomic databases

    PaxDbiQ9FNA2.
    PRIDEiQ9FNA2.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT5G13700.1; AT5G13700.1; AT5G13700.
    GeneIDi831215.
    GrameneiAT5G13700.1; AT5G13700.1; AT5G13700.
    KEGGiath:AT5G13700.

    Organism-specific databases

    TAIRiAT5G13700.

    Phylogenomic databases

    eggNOGiKOG0029. Eukaryota.
    ENOG410XSNC. LUCA.
    HOGENOMiHOG000174927.
    InParanoidiQ9FNA2.
    KOiK13366.
    OMAiETCERTK.
    OrthoDBiEOG09360ACK.
    PhylomeDBiQ9FNA2.

    Enzyme and pathway databases

    UniPathwayiUPA00211.
    BioCyciARA:AT5G13700-MONOMER.
    MetaCyc:AT5G13700-MONOMER.
    BRENDAi1.5.3.16. 399.

    Miscellaneous databases

    PROiQ9FNA2.

    Gene expression databases

    GenevisibleiQ9FNA2. AT.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002937. Amino_oxidase.
    IPR023753. FAD/NAD-binding_dom.
    IPR001613. Flavin_amine_oxidase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 2 hits.
    [Graphical view]
    PRINTSiPR00757. AMINEOXDASEF.
    SUPFAMiSSF51905. SSF51905. 2 hits.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPAO1_ARATH
    AccessioniPrimary (citable) accession number: Q9FNA2
    Secondary accession number(s): Q7FL79
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 14, 2008
    Last sequence update: March 1, 2001
    Last modified: September 7, 2016
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.