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Protein

ATP-dependent Clp protease proteolytic subunit 2, mitochondrial

Gene

CLPP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the mitochondrial ATP-dependent Clp protease (PubMed:11352464). Cleaves peptides in various proteins in a process that requires ATP hydrolysis (By similarity). Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity).By similarity1 Publication

Catalytic activityi

Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei127 – 1271NucleophileBy similarity
Active sitei152 – 1521By similarity

GO - Molecular functioni

  • cobalt ion binding Source: TAIR
  • serine-type endopeptidase activity Source: InterPro
  • zinc ion binding Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciARA:AT5G23140-MONOMER.

Protein family/group databases

MEROPSiS14.A02.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent Clp protease proteolytic subunit 2, mitochondrial1 Publication (EC:3.4.21.92)
Alternative name(s):
ATP-dependent Clp protease proteolytic subunit 1, mitochondrial
Endopeptidase ClpP21 Publication
nClpP7
Gene namesi
Name:CLPP21 Publication
Synonyms:NCLPP7
Ordered Locus Names:At5g23140Imported
ORF Names:MYJ24.13Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G23140.

Subcellular locationi

GO - Cellular componenti

  • chloroplastic endopeptidase Clp complex Source: TAIR
  • chloroplast thylakoid membrane Source: TAIR
  • mitochondrion Source: TAIR
  • plastid stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3030MitochondrionSequence analysisAdd
BLAST
Chaini31 – 241211ATP-dependent Clp protease proteolytic subunit 2, mitochondrialPRO_0000308977Add
BLAST

Proteomic databases

PaxDbiQ9FN42.
PRIDEiQ9FN42.

Expressioni

Tissue specificityi

Constitutively expressed in leaves, shoots, roots and flowers.1 Publication

Gene expression databases

GenevisibleiQ9FN42. AT.

Interactioni

Subunit structurei

Homotetradecamer.1 Publication

Protein-protein interaction databases

STRINGi3702.AT5G23140.1.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R8Vmodel-A1-241[»]
ProteinModelPortaliQ9FN42.
SMRiQ9FN42. Positions 47-222.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S14 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0840. Eukaryota.
COG0740. LUCA.
HOGENOMiHOG000285833.
InParanoidiQ9FN42.
KOiK01358.
OMAiNMDRDHF.
OrthoDBiEOG09360LJE.
PhylomeDBiQ9FN42.

Family and domain databases

Gene3Di3.90.226.10. 1 hit.
HAMAPiMF_00444. ClpP. 1 hit.
InterProiIPR001907. ClpP.
IPR029045. ClpP/crotonase-like_dom.
IPR023562. ClpP/TepA.
IPR033135. ClpP_His_AS.
IPR018215. ClpP_Ser_AS.
[Graphical view]
PANTHERiPTHR10381. PTHR10381. 1 hit.
PfamiPF00574. CLP_protease. 1 hit.
[Graphical view]
PRINTSiPR00127. CLPPROTEASEP.
SUPFAMiSSF52096. SSF52096. 1 hit.
PROSITEiPS00382. CLP_PROTEASE_HIS. 1 hit.
PS00381. CLP_PROTEASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FN42-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMRGLVSGAK MLSSTPSSMA TSIATGRRSY SLIPMVIEHS SRGERAYDIF
60 70 80 90 100
SRLLKERIIC INGPINDDTS HVVVAQLLYL ESENPSKPIH MYLNSPGGHV
110 120 130 140 150
TAGLAIYDTM QYIRSPISTI CLGQAASMAS LLLAAGAKGQ RRSLPNATVM
160 170 180 190 200
IHQPSGGYSG QAKDITIHTK QIVRVWDALN ELYVKHTGQP LDVVANNMDR
210 220 230 240
DHFMTPEEAK AFGIIDEVID ERPLELVKDA VGNESKDKSS S
Length:241
Mass (Da):26,283
Last modified:March 1, 2001 - v1
Checksum:iDFC93372387E542A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006708 Genomic DNA. Translation: BAB09831.1.
CP002688 Genomic DNA. Translation: AED93126.1.
AK118523 mRNA. Translation: BAC43126.1.
BT005261 mRNA. Translation: AAO63325.1.
RefSeqiNP_568427.1. NM_122220.4.
UniGeneiAt.31024.

Genome annotation databases

EnsemblPlantsiAT5G23140.1; AT5G23140.1; AT5G23140.
GeneIDi832378.
GrameneiAT5G23140.1; AT5G23140.1; AT5G23140.
KEGGiath:AT5G23140.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006708 Genomic DNA. Translation: BAB09831.1.
CP002688 Genomic DNA. Translation: AED93126.1.
AK118523 mRNA. Translation: BAC43126.1.
BT005261 mRNA. Translation: AAO63325.1.
RefSeqiNP_568427.1. NM_122220.4.
UniGeneiAt.31024.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R8Vmodel-A1-241[»]
ProteinModelPortaliQ9FN42.
SMRiQ9FN42. Positions 47-222.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G23140.1.

Protein family/group databases

MEROPSiS14.A02.

Proteomic databases

PaxDbiQ9FN42.
PRIDEiQ9FN42.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G23140.1; AT5G23140.1; AT5G23140.
GeneIDi832378.
GrameneiAT5G23140.1; AT5G23140.1; AT5G23140.
KEGGiath:AT5G23140.

Organism-specific databases

TAIRiAT5G23140.

Phylogenomic databases

eggNOGiKOG0840. Eukaryota.
COG0740. LUCA.
HOGENOMiHOG000285833.
InParanoidiQ9FN42.
KOiK01358.
OMAiNMDRDHF.
OrthoDBiEOG09360LJE.
PhylomeDBiQ9FN42.

Enzyme and pathway databases

BioCyciARA:AT5G23140-MONOMER.

Miscellaneous databases

PROiQ9FN42.

Gene expression databases

GenevisibleiQ9FN42. AT.

Family and domain databases

Gene3Di3.90.226.10. 1 hit.
HAMAPiMF_00444. ClpP. 1 hit.
InterProiIPR001907. ClpP.
IPR029045. ClpP/crotonase-like_dom.
IPR023562. ClpP/TepA.
IPR033135. ClpP_His_AS.
IPR018215. ClpP_Ser_AS.
[Graphical view]
PANTHERiPTHR10381. PTHR10381. 1 hit.
PfamiPF00574. CLP_protease. 1 hit.
[Graphical view]
PRINTSiPR00127. CLPPROTEASEP.
SUPFAMiSSF52096. SSF52096. 1 hit.
PROSITEiPS00382. CLP_PROTEASE_HIS. 1 hit.
PS00381. CLP_PROTEASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLPP2_ARATH
AccessioniPrimary (citable) accession number: Q9FN42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.