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Protein

ATP-dependent Clp protease proteolytic subunit 2, mitochondrial

Gene

CLPP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the mitochondrial ATP-dependent Clp protease (PubMed:11352464). Cleaves peptides in various proteins in a process that requires ATP hydrolysis (By similarity). Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity).By similarity1 Publication

Catalytic activityi

Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei127NucleophileBy similarity1
Active sitei152By similarity1

GO - Molecular functioni

  • cobalt ion binding Source: TAIR
  • serine-type endopeptidase activity Source: InterPro
  • zinc ion binding Source: TAIR

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS14.A02.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent Clp protease proteolytic subunit 2, mitochondrial1 Publication (EC:3.4.21.92)
Alternative name(s):
ATP-dependent Clp protease proteolytic subunit 1, mitochondrial
Endopeptidase ClpP21 Publication
nClpP7
Gene namesi
Name:CLPP21 Publication
Synonyms:NCLPP7
Ordered Locus Names:At5g23140Imported
ORF Names:MYJ24.13Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G23140.
TAIRilocus:2178282. AT5G23140.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 30Mitochondrion1 PublicationAdd BLAST30
ChainiPRO_000030897731 – 241ATP-dependent Clp protease proteolytic subunit 2, mitochondrialAdd BLAST211

Proteomic databases

PaxDbiQ9FN42.
PRIDEiQ9FN42.

Expressioni

Tissue specificityi

Constitutively expressed in leaves, shoots, roots and flowers.1 Publication

Gene expression databases

ExpressionAtlasiQ9FN42. baseline and differential.
GenevisibleiQ9FN42. AT.

Interactioni

Subunit structurei

Homotetradecamer.1 Publication

Protein-protein interaction databases

STRINGi3702.AT5G23140.1.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R8Vmodel-A1-241[»]
ProteinModelPortaliQ9FN42.
SMRiQ9FN42.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S14 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0840. Eukaryota.
COG0740. LUCA.
HOGENOMiHOG000285833.
InParanoidiQ9FN42.
KOiK01358.
OMAiLFLQSEN.
OrthoDBiEOG09360LJE.
PhylomeDBiQ9FN42.

Family and domain databases

CDDicd07017. S14_ClpP_2. 1 hit.
HAMAPiMF_00444. ClpP. 1 hit.
InterProiView protein in InterPro
IPR001907. ClpP.
IPR029045. ClpP/crotonase-like_dom_sf.
IPR023562. ClpP/TepA.
IPR033135. ClpP_His_AS.
IPR018215. ClpP_Ser_AS.
PANTHERiPTHR10381. PTHR10381. 1 hit.
PfamiView protein in Pfam
PF00574. CLP_protease. 1 hit.
PRINTSiPR00127. CLPPROTEASEP.
SUPFAMiSSF52096. SSF52096. 1 hit.
PROSITEiView protein in PROSITE
PS00382. CLP_PROTEASE_HIS. 1 hit.
PS00381. CLP_PROTEASE_SER. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FN42-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMRGLVSGAK MLSSTPSSMA TSIATGRRSY SLIPMVIEHS SRGERAYDIF
60 70 80 90 100
SRLLKERIIC INGPINDDTS HVVVAQLLYL ESENPSKPIH MYLNSPGGHV
110 120 130 140 150
TAGLAIYDTM QYIRSPISTI CLGQAASMAS LLLAAGAKGQ RRSLPNATVM
160 170 180 190 200
IHQPSGGYSG QAKDITIHTK QIVRVWDALN ELYVKHTGQP LDVVANNMDR
210 220 230 240
DHFMTPEEAK AFGIIDEVID ERPLELVKDA VGNESKDKSS S
Length:241
Mass (Da):26,283
Last modified:March 1, 2001 - v1
Checksum:iDFC93372387E542A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006708 Genomic DNA. Translation: BAB09831.1.
CP002688 Genomic DNA. Translation: AED93126.1.
AK118523 mRNA. Translation: BAC43126.1.
BT005261 mRNA. Translation: AAO63325.1.
RefSeqiNP_568427.1. NM_122220.5.
UniGeneiAt.31024.

Genome annotation databases

EnsemblPlantsiAT5G23140.1; AT5G23140.1; AT5G23140.
GeneIDi832378.
GrameneiAT5G23140.1; AT5G23140.1; AT5G23140.
KEGGiath:AT5G23140.

Similar proteinsi

Entry informationi

Entry nameiCLPP2_ARATH
AccessioniPrimary (citable) accession number: Q9FN42
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: March 1, 2001
Last modified: November 22, 2017
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families