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Protein

Beta-galactosidase 10

Gene

BGAL10

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei188Proton donorSequence analysis1
Active sitei257NucleophileSequence analysis1

GO - Molecular functioni

  • beta-galactosidase activity Source: CACAO

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • response to karrikin Source: TAIR

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT5G63810-MONOMER

Protein family/group databases

CAZyiGH35 Glycoside Hydrolase Family 35

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 10 (EC:3.2.1.23)
Short name:
Lactase 10
Gene namesi
Name:BGAL10
Ordered Locus Names:At5g63810
ORF Names:MGI19.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G63810
TAIRilocus:2163951 AT5G63810

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_500006588430 – 741Beta-galactosidase 10Add BLAST712

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi31N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi358N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi396N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi469N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi525N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi583N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9FN08
PRIDEiQ9FN08

Expressioni

Tissue specificityi

Ubiquitous.2 Publications

Gene expression databases

ExpressionAtlasiQ9FN08 baseline and differential
GenevisibleiQ9FN08 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G63810.1

Structurei

3D structure databases

ProteinModelPortaliQ9FN08
SMRiQ9FN08
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0496 Eukaryota
COG1874 LUCA
HOGENOMiHOG000239919
InParanoidiQ9FN08
OMAiHKSIKLG
OrthoDBiEOG093602V4
PhylomeDBiQ9FN08

Family and domain databases

Gene3Di2.60.120.260, 2 hits
InterProiView protein in InterPro
IPR008979 Galactose-bd-like_sf
IPR031330 Gly_Hdrlase_35_cat
IPR019801 Glyco_hydro_35_CS
IPR001944 Glycoside_Hdrlase_35
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR23421 PTHR23421, 1 hit
PfamiView protein in Pfam
PF01301 Glyco_hydro_35, 1 hit
PRINTSiPR00742 GLHYDRLASE35
SUPFAMiSSF49785 SSF49785, 3 hits
SSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS01182 GLYCOSYL_HYDROL_F35, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9FN08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRVTTESIA STAILVVMVF LFSWRSIEAA NVSYDHRSLT IGNRRQLIIS
60 70 80 90 100
AAIHYPRSVP AMWPSLVQTA KEGGCNAIES YVFWNGHEPS PGKYYFGGRY
110 120 130 140 150
NIVKFIKIVQ QAGMHMILRI GPFVAAEWNY GGVPVWLHYV PGTVFRADNE
160 170 180 190 200
PWKHYMESFT TYIVNLLKQE KLFAPQGGPI ILSQVENEYG YYEKDYGEGG
210 220 230 240 250
KRYAQWSASM AVSQNIGVPW MMCQQWDAPP TVISTCNGFY CDQFTPNTPD
260 270 280 290 300
KPKIWTENWP GWFKTFGGRD PHRPAEDVAY SVARFFGKGG SVHNYYMYHG
310 320 330 340 350
GTNFGRTSGG PFITTSYDYE APIDEYGLPR LPKWGHLKDL HKAIMLSENL
360 370 380 390 400
LISGEHQNFT LGHSLEADVY TDSSGTCAAF LSNLDDKNDK AVMFRNTSYH
410 420 430 440 450
LPAWSVSILP DCKTEVFNTA KVTSKSSKVE MLPEDLKSSS GLKWEVFSEK
460 470 480 490 500
PGIWGAADFV KNELVDHINT TKDTTDYLWY TTSITVSENE AFLKKGSSPV
510 520 530 540 550
LFIESKGHTL HVFINKEYLG TATGNGTHVP FKLKKPVALK AGENNIDLLS
560 570 580 590 600
MTVGLANAGS FYEWVGAGLT SVSIKGFNKG TLNLTNSKWS YKLGVEGEHL
610 620 630 640 650
ELFKPGNSGA VKWTVTTKPP KKQPLTWYKV VIEPPSGSEP VGLDMISMGK
660 670 680 690 700
GMAWLNGEEI GRYWPRIARK NSPNDECVKE CDYRGKFMPD KCLTGCGEPS
710 720 730 740
QRWYHVPRSW FKSSGNELVI FEEKGGNPMK IKLSKRKVSV V
Length:741
Mass (Da):83,102
Last modified:March 1, 2001 - v1
Checksum:i6BEE67970375CE98
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti544N → T in CAB64746 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ270306 mRNA Translation: CAB64746.1
AB007646 Genomic DNA Translation: BAB11029.1
CP002688 Genomic DNA Translation: AED97800.1
AY093118 mRNA Translation: AAM13117.1
BT010338 mRNA Translation: AAQ56781.1
RefSeqiNP_201186.1, NM_125776.2
UniGeneiAt.705

Genome annotation databases

EnsemblPlantsiAT5G63810.1; AT5G63810.1; AT5G63810
GeneIDi836501
GrameneiAT5G63810.1; AT5G63810.1; AT5G63810
KEGGiath:AT5G63810

Similar proteinsi

Entry informationi

Entry nameiBGA10_ARATH
AccessioniPrimary (citable) accession number: Q9FN08
Secondary accession number(s): Q9SCV2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health