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Q9FN08 (BGA10_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase 10

Short name=Lactase 10
EC=3.2.1.23
Gene names
Name:BGAL10
Ordered Locus Names:At5g63810
ORF Names:MGI19.1
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length741 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Secretedextracellular spaceapoplast Potential.

Tissue specificity

Ubiquitous. Ref.5 Ref.6

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Potential
Chain30 – 741712Beta-galactosidase 10
PRO_5000065884

Sites

Active site1881Proton donor Potential
Active site2571Nucleophile Potential

Amino acid modifications

Glycosylation311N-linked (GlcNAc...) Potential
Glycosylation3581N-linked (GlcNAc...) Potential
Glycosylation3961N-linked (GlcNAc...) Potential
Glycosylation4691N-linked (GlcNAc...) Potential
Glycosylation5251N-linked (GlcNAc...) Potential
Glycosylation5831N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict5441N → T in CAB64746. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9FN08 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 6BEE67970375CE98

FASTA74183,102
        10         20         30         40         50         60 
MNRVTTESIA STAILVVMVF LFSWRSIEAA NVSYDHRSLT IGNRRQLIIS AAIHYPRSVP 

        70         80         90        100        110        120 
AMWPSLVQTA KEGGCNAIES YVFWNGHEPS PGKYYFGGRY NIVKFIKIVQ QAGMHMILRI 

       130        140        150        160        170        180 
GPFVAAEWNY GGVPVWLHYV PGTVFRADNE PWKHYMESFT TYIVNLLKQE KLFAPQGGPI 

       190        200        210        220        230        240 
ILSQVENEYG YYEKDYGEGG KRYAQWSASM AVSQNIGVPW MMCQQWDAPP TVISTCNGFY 

       250        260        270        280        290        300 
CDQFTPNTPD KPKIWTENWP GWFKTFGGRD PHRPAEDVAY SVARFFGKGG SVHNYYMYHG 

       310        320        330        340        350        360 
GTNFGRTSGG PFITTSYDYE APIDEYGLPR LPKWGHLKDL HKAIMLSENL LISGEHQNFT 

       370        380        390        400        410        420 
LGHSLEADVY TDSSGTCAAF LSNLDDKNDK AVMFRNTSYH LPAWSVSILP DCKTEVFNTA 

       430        440        450        460        470        480 
KVTSKSSKVE MLPEDLKSSS GLKWEVFSEK PGIWGAADFV KNELVDHINT TKDTTDYLWY 

       490        500        510        520        530        540 
TTSITVSENE AFLKKGSSPV LFIESKGHTL HVFINKEYLG TATGNGTHVP FKLKKPVALK 

       550        560        570        580        590        600 
AGENNIDLLS MTVGLANAGS FYEWVGAGLT SVSIKGFNKG TLNLTNSKWS YKLGVEGEHL 

       610        620        630        640        650        660 
ELFKPGNSGA VKWTVTTKPP KKQPLTWYKV VIEPPSGSEP VGLDMISMGK GMAWLNGEEI 

       670        680        690        700        710        720 
GRYWPRIARK NSPNDECVKE CDYRGKFMPD KCLTGCGEPS QRWYHVPRSW FKSSGNELVI 

       730        740 
FEEKGGNPMK IKLSKRKVSV V 

« Hide

References

« Hide 'large scale' references
[1]"The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana."
Gy I., Kreis M., Lecharny A.
Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana."
Iglesias N., Abelenda J.A., Rodino M., Sampedro J., Revilla G., Zarra I.
Plant Cell Physiol. 47:55-63(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35."
Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.
Phytochemistry 68:1510-1520(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ270306 mRNA. Translation: CAB64746.1.
AB007646 Genomic DNA. Translation: BAB11029.1.
CP002688 Genomic DNA. Translation: AED97800.1.
AY093118 mRNA. Translation: AAM13117.1.
BT010338 mRNA. Translation: AAQ56781.1.
RefSeqNP_201186.1. NM_125776.2.
UniGeneAt.705.

3D structure databases

ProteinModelPortalQ9FN08.
SMRQ9FN08. Positions 32-399, 501-548.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

Proteomic databases

PaxDbQ9FN08.
PRIDEQ9FN08.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G63810.1; AT5G63810.1; AT5G63810.
GeneID836501.
KEGGath:AT5G63810.

Organism-specific databases

GeneFarm512. 90.
TAIRAT5G63810.

Phylogenomic databases

eggNOGCOG1874.
HOGENOMHOG000239919.
InParanoidQ9FN08.
OMAHINTTKD.
PhylomeDBQ9FN08.
ProtClustDBCLSN2916932.

Enzyme and pathway databases

BioCycARA:AT5G63810-MONOMER.

Gene expression databases

GenevestigatorQ9FN08.

Family and domain databases

Gene3D2.60.120.260. 3 hits.
3.20.20.80. 1 hit.
InterProIPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF49785. SSF49785. 3 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGA10_ARATH
AccessionPrimary (citable) accession number: Q9FN08
Secondary accession number(s): Q9SCV2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names