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Q9FN02

- PPP7_ARATH

UniProt

Q9FN02 - PPP7_ARATH

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Protein

Serine/threonine-protein phosphatase 7

Gene

PP7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Phosphatase active on para-nitrophenylphosphate (pNPP) and on various phosphoproteins such as myelin basic protein. Seems to act as a positive regulator of cryptochrome signaling involved in hypocotyl growth inhibition and cotyledon expansion under white and blue light conditions. Confers thermotolerance. Required for heat shock mediated-signaling pathway that leads to the expression of heat shock proteins (HSPs).3 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+1 PublicationNote: Binds 2 manganese ions per subunit.1 Publication

Enzyme regulationi

Inhibited by NaF and orthovanadate, as well as by divalent cations such as Ni2+ and Zn2+. Inhibited by polylysine with myelin basic protein as substrate, but activated by polylysine with pNPP as substrate. Reversibly regulated by redox agents. Inhibited by submillimolar Pi concentrations. Slightly repressed by calmodulin (CaM).1 Publication

Kineticsi

  1. KM=1.7 mM for pNPP (at pH 7.8 and 25 degrees Celsius)2 Publications

pH dependencei

Optimum pH is around 7.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi84 – 841Manganese 1By similarity
Metal bindingi86 – 861Manganese 1By similarity
Metal bindingi113 – 1131Manganese 1By similarity
Metal bindingi113 – 1131Manganese 2By similarity
Metal bindingi145 – 1451Manganese 2By similarity
Active sitei146 – 1461Proton donorBy similarity
Metal bindingi197 – 1971Manganese 2By similarity
Metal bindingi303 – 3031Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: TAIR

GO - Biological processi

  1. blue light signaling pathway Source: TAIR
  2. dephosphorylation Source: GOC
  3. response to heat Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G63870-MONOMER.
ARA:GQT-397-MONOMER.
ARA:GQT-398-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 7 (EC:3.1.3.16)
Gene namesi
Name:PP7
Ordered Locus Names:At5g63870
ORF Names:MGI19.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G63870.

Subcellular locationi

Nucleusnucleoplasm 1 Publication

GO - Cellular componenti

  1. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi207 – 23125PKRTT…SSMKL → GGGGGGGGGGGGGGGGGGGG GGGGG: Normal subcellular location. 1 PublicationAdd
BLAST
Mutagenesisi209 – 23123Missing: Reduced activity, but enhanced inducibility by polylysine with pNPP as substrate; reduced binding with calmodulin. 1 PublicationAdd
BLAST
Mutagenesisi369 – 41345Missing: Loss of activity; normal binding with calmodulin; cytoplasmic instead of nuclear subcellular location. 2 PublicationsAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 413413Serine/threonine-protein phosphatase 7PRO_0000308991Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 671 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9FN02.
PRIDEiQ9FN02.

Expressioni

Tissue specificityi

Expressed in leaves, and, to a lower extent, in stems and flowers.1 Publication

Inductioni

By heat shock.1 Publication

Gene expression databases

GenevestigatoriQ9FN02.

Interactioni

Subunit structurei

Monomer, homodimer, and heteropolymer. Interacts with calmodulin (CaM3 and CaM4) and HSFA1A/HSF1.3 Publications

Protein-protein interaction databases

BioGridi21749. 4 interactions.
MINTiMINT-222502.

Structurei

3D structure databases

ProteinModelPortaliQ9FN02.
SMRiQ9FN02. Positions 52-385.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-7 subfamily.Curated

Phylogenomic databases

eggNOGiCOG0639.
HOGENOMiHOG000242900.
KOiK15423.
OMAiTIIAGCV.
PhylomeDBiQ9FN02.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 2 hits.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9FN02-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METVPPSPIT WPDGGALTND WVHGLMSCFE WSSWNLPPSQ LPSLLPVNVF
60 70 80 90 100
DSLVLTAHKI LHKERNCVHI DDLDSVSNVV VVGDIHGQLH DLLFLLKDTG
110 120 130 140 150
FPCQNRCYVF NGDYVDRGAW GLETFLVLLS WKVLMPDRVY LLRGNHESKY
160 170 180 190 200
CTSMYGFEKE VLTKYGDKGK HVYRKCLGCF EGLPLASIIS GRVYTAHGGL
210 220 230 240 250
FRSPVLPKRT TRGKKNRRVV LLEPEPSSMK LGTLDELMQA RRSVLDPPWE
260 270 280 290 300
GSNLIPGDVL WSDPSMTPGL SPNEQRGIGL LWGPDCTEDF LKKYELKLII
310 320 330 340 350
RSHEGPDARE KRTGLGGMDN GYTIDHNVES GKLITIFSAP DYPQFQATEE
360 370 380 390 400
RYKNKGAYII LQAPDFSDPQ FHSFEAVKPR PKAHPYYDFE NVIDSDDEMD
410
KSAMDTNNEQ PNS
Length:413
Mass (Da):46,619
Last modified:March 1, 2001 - v1
Checksum:i111A9E01A222A058
GO
Isoform 2 (identifier: Q9FN02-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     298-301: LIIR → VTPK
     302-413: Missing.

Note: May be due to an intron retention. No experimental confirmation available.

Show »
Length:301
Mass (Da):33,880
Checksum:iA8D09318702B0C62
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti266 – 2661M → I in CAA03886. (PubMed:9584984)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei298 – 3014LIIR → VTPK in isoform 2. 1 PublicationVSP_029088
Alternative sequencei302 – 413112Missing in isoform 2. 1 PublicationVSP_029089Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000057 mRNA. Translation: CAA03886.1.
AB007646 Genomic DNA. Translation: BAB11035.1.
CP002688 Genomic DNA. Translation: AED97806.1.
CP002688 Genomic DNA. Translation: AED97807.1.
CP002688 Genomic DNA. Translation: AED97808.1.
AY050898 mRNA. No translation available.
PIRiT51611.
RefSeqiNP_568979.1. NM_125782.3. [Q9FN02-2]
NP_851258.2. NM_180927.3. [Q9FN02-1]
NP_851259.1. NM_180928.1. [Q9FN02-1]
UniGeneiAt.20661.

Genome annotation databases

EnsemblPlantsiAT5G63870.1; AT5G63870.1; AT5G63870. [Q9FN02-1]
AT5G63870.2; AT5G63870.2; AT5G63870. [Q9FN02-1]
GeneIDi836507.
KEGGiath:AT5G63870.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000057 mRNA. Translation: CAA03886.1 .
AB007646 Genomic DNA. Translation: BAB11035.1 .
CP002688 Genomic DNA. Translation: AED97806.1 .
CP002688 Genomic DNA. Translation: AED97807.1 .
CP002688 Genomic DNA. Translation: AED97808.1 .
AY050898 mRNA. No translation available.
PIRi T51611.
RefSeqi NP_568979.1. NM_125782.3. [Q9FN02-2 ]
NP_851258.2. NM_180927.3. [Q9FN02-1 ]
NP_851259.1. NM_180928.1. [Q9FN02-1 ]
UniGenei At.20661.

3D structure databases

ProteinModelPortali Q9FN02.
SMRi Q9FN02. Positions 52-385.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 21749. 4 interactions.
MINTi MINT-222502.

Proteomic databases

PaxDbi Q9FN02.
PRIDEi Q9FN02.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G63870.1 ; AT5G63870.1 ; AT5G63870 . [Q9FN02-1 ]
AT5G63870.2 ; AT5G63870.2 ; AT5G63870 . [Q9FN02-1 ]
GeneIDi 836507.
KEGGi ath:AT5G63870.

Organism-specific databases

TAIRi AT5G63870.

Phylogenomic databases

eggNOGi COG0639.
HOGENOMi HOG000242900.
KOi K15423.
OMAi TIIAGCV.
PhylomeDBi Q9FN02.

Enzyme and pathway databases

BioCyci ARA:AT5G63870-MONOMER.
ARA:GQT-397-MONOMER.
ARA:GQT-398-MONOMER.

Gene expression databases

Genevestigatori Q9FN02.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 2 hits.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "PP7, a plant phosphatase representing a novel evolutionary branch of eukaryotic protein Ser/Thr phosphatases."
    Andreeva A.V., Evans D.E., Hawes C.R., Bennett N., Kutuzov M.A.
    Biochem. Mol. Biol. Int. 44:703-715(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
    Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  5. "Expression and characterization of PP7, a novel plant protein Ser/Thr phosphatase distantly related to RdgC/PPEF and PP5."
    Kutuzov M.A., Evans D.E., Andreeva A.V.
    FEBS Lett. 440:147-152(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INHIBITION, DISULFIDE BOND, COFACTOR.
  6. "Purification of plant protein phosphatase PP7 and evidence for its redox regulation."
    Andreeva A.V., Solov'eva O.V., Kakuev D.L., Kutuzov M.A.
    Arch. Biochem. Biophys. 396:65-70(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION, SUBUNIT.
  7. "Noncompetitive inhibition of plant protein Ser/Thr phosphatase PP7 by phosphate."
    Kutuzov M.A., Andreeva A.V.
    Biochem. Biophys. Res. Commun. 283:93-96(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, INHIBITION BY INORGANIC PHOSPHATE.
  8. "Interaction of plant protein Ser/Thr phosphatase PP7 with calmodulin."
    Kutuzov M.A., Bennett N., Andreeva A.V.
    Biochem. Biophys. Res. Commun. 289:634-640(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CALMODULIN, MUTAGENESIS OF 209-ARG--LEU-231 AND 369-PRO--SER-413.
  9. "Nuclear localization of the plant protein Ser/Thr phosphatase PP7."
    Andreeva A.V., Kutuzov M.A.
    Mol. Cell Biol. Res. Commun. 4:345-352(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF 207-PRO--LEU-231 AND 369-PRO--SER-413, SUBCELLULAR LOCATION.
  10. "PP7 is a positive regulator of blue light signaling in Arabidopsis."
    Moeller S.G., Kim Y.-S., Kunkel T., Chua N.-H.
    Plant Cell 15:1111-1119(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  11. "Calmodulin-binding protein phosphatase PP7 is involved in thermotolerance in Arabidopsis."
    Liu H.-T., Li G.-L., Chang H., Sun D.-Y., Zhou R.-G., Li B.
    Plant Cell Environ. 30:156-164(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CALMODULIN AND HSFA1A, INDUCTION BY HEAT SHOCK.
  12. "Arabidopsis PPP family of serine/threonine phosphatases."
    Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C.
    Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPPP7_ARATH
AccessioniPrimary (citable) accession number: Q9FN02
Secondary accession number(s): O49346, Q3E865
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: March 1, 2001
Last modified: November 26, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3