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Protein

Serine/threonine-protein phosphatase 7

Gene

PP7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphatase active on para-nitrophenylphosphate (pNPP) and on various phosphoproteins such as myelin basic protein. Seems to act as a positive regulator of cryptochrome signaling involved in hypocotyl growth inhibition and cotyledon expansion under white and blue light conditions. Confers thermotolerance. Required for heat shock mediated-signaling pathway that leads to the expression of heat shock proteins (HSPs).3 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+1 PublicationNote: Binds 2 manganese ions per subunit.1 Publication

Enzyme regulationi

Inhibited by NaF and orthovanadate, as well as by divalent cations such as Ni2+ and Zn2+. Inhibited by polylysine with myelin basic protein as substrate, but activated by polylysine with pNPP as substrate. Reversibly regulated by redox agents. Inhibited by submillimolar Pi concentrations. Slightly repressed by calmodulin (CaM).1 Publication

Kineticsi

  1. KM=1.7 mM for pNPP (at pH 7.8 and 25 degrees Celsius)2 Publications

    pH dependencei

    Optimum pH is around 7.2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi84Manganese 1By similarity1
    Metal bindingi86Manganese 1By similarity1
    Metal bindingi113Manganese 1By similarity1
    Metal bindingi113Manganese 2By similarity1
    Metal bindingi145Manganese 2By similarity1
    Active sitei146Proton donorBy similarity1
    Metal bindingi197Manganese 2By similarity1
    Metal bindingi303Manganese 2By similarity1

    GO - Molecular functioni

    • metal ion binding Source: UniProtKB-KW
    • protein serine/threonine phosphatase activity Source: TAIR

    GO - Biological processi

    • blue light signaling pathway Source: TAIR
    • response to heat Source: UniProtKB

    Keywordsi

    Molecular functionHydrolase, Protein phosphatase
    LigandManganese, Metal-binding

    Enzyme and pathway databases

    BRENDAi3.1.3.16. 399.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 7 (EC:3.1.3.16)
    Gene namesi
    Name:PP7
    Ordered Locus Names:At5g63870
    ORF Names:MGI19.7
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    AraportiAT5G63870.
    TAIRilocus:2163991. AT5G63870.

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi207 – 231PKRTT…SSMKL → GGGGGGGGGGGGGGGGGGGG GGGGG: Normal subcellular location. 1 PublicationAdd BLAST25
    Mutagenesisi209 – 231Missing : Reduced activity, but enhanced inducibility by polylysine with pNPP as substrate; reduced binding with calmodulin. 1 PublicationAdd BLAST23
    Mutagenesisi369 – 413Missing : Loss of activity; normal binding with calmodulin; cytoplasmic instead of nuclear subcellular location. 2 PublicationsAdd BLAST45

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003089911 – 413Serine/threonine-protein phosphatase 7Add BLAST413

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi28 ↔ 671 Publication

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiQ9FN02.
    PRIDEiQ9FN02.

    PTM databases

    iPTMnetiQ9FN02.

    Expressioni

    Tissue specificityi

    Expressed in leaves, and, to a lower extent, in stems and flowers.1 Publication

    Inductioni

    By heat shock.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9FN02. baseline and differential.
    GenevisibleiQ9FN02. AT.

    Interactioni

    Subunit structurei

    Monomer, homodimer, and heteropolymer. Interacts with calmodulin (CaM3 and CaM4) and HSFA1A/HSF1.3 Publications

    Protein-protein interaction databases

    BioGridi21749. 5 interactors.
    IntActiQ9FN02. 1 interactor.
    MINTiMINT-222502.
    STRINGi3702.AT5G63870.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9FN02.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-7 subfamily.Curated

    Phylogenomic databases

    eggNOGiKOG0376. Eukaryota.
    COG0639. LUCA.
    HOGENOMiHOG000242900.
    KOiK15423.
    OMAiPPWEGTN.
    OrthoDBiEOG09360AIN.
    PhylomeDBiQ9FN02.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiView protein in InterPro
    IPR004843. Calcineurin-like_PHP_ApaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    PfamiView protein in Pfam
    PF00149. Metallophos. 1 hit.
    PRINTSiPR00114. STPHPHTASE.
    SMARTiView protein in SMART
    SM00156. PP2Ac. 1 hit.
    SUPFAMiSSF56300. SSF56300. 2 hits.
    PROSITEiView protein in PROSITE
    PS00125. SER_THR_PHOSPHATASE. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q9FN02-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    METVPPSPIT WPDGGALTND WVHGLMSCFE WSSWNLPPSQ LPSLLPVNVF
    60 70 80 90 100
    DSLVLTAHKI LHKERNCVHI DDLDSVSNVV VVGDIHGQLH DLLFLLKDTG
    110 120 130 140 150
    FPCQNRCYVF NGDYVDRGAW GLETFLVLLS WKVLMPDRVY LLRGNHESKY
    160 170 180 190 200
    CTSMYGFEKE VLTKYGDKGK HVYRKCLGCF EGLPLASIIS GRVYTAHGGL
    210 220 230 240 250
    FRSPVLPKRT TRGKKNRRVV LLEPEPSSMK LGTLDELMQA RRSVLDPPWE
    260 270 280 290 300
    GSNLIPGDVL WSDPSMTPGL SPNEQRGIGL LWGPDCTEDF LKKYELKLII
    310 320 330 340 350
    RSHEGPDARE KRTGLGGMDN GYTIDHNVES GKLITIFSAP DYPQFQATEE
    360 370 380 390 400
    RYKNKGAYII LQAPDFSDPQ FHSFEAVKPR PKAHPYYDFE NVIDSDDEMD
    410
    KSAMDTNNEQ PNS
    Length:413
    Mass (Da):46,619
    Last modified:March 1, 2001 - v1
    Checksum:i111A9E01A222A058
    GO
    Isoform 2 (identifier: Q9FN02-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         298-301: LIIR → VTPK
         302-413: Missing.

    Note: May be due to an intron retention. No experimental confirmation available.
    Show »
    Length:301
    Mass (Da):33,880
    Checksum:iA8D09318702B0C62
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti266M → I in CAA03886 (PubMed:9584984).Curated1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_029088298 – 301LIIR → VTPK in isoform 2. 1 Publication4
    Alternative sequenceiVSP_029089302 – 413Missing in isoform 2. 1 PublicationAdd BLAST112

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ000057 mRNA. Translation: CAA03886.1.
    AB007646 Genomic DNA. Translation: BAB11035.1.
    CP002688 Genomic DNA. Translation: AED97806.1.
    CP002688 Genomic DNA. Translation: AED97807.1.
    CP002688 Genomic DNA. Translation: AED97808.1.
    AY050898 mRNA. No translation available.
    PIRiT51611.
    RefSeqiNP_568979.1. NM_125782.3. [Q9FN02-2]
    NP_851258.2. NM_180927.4. [Q9FN02-1]
    NP_851259.1. NM_180928.2. [Q9FN02-1]
    UniGeneiAt.20661.

    Genome annotation databases

    EnsemblPlantsiAT5G63870.1; AT5G63870.1; AT5G63870. [Q9FN02-1]
    AT5G63870.2; AT5G63870.2; AT5G63870. [Q9FN02-1]
    AT5G63870.3; AT5G63870.3; AT5G63870. [Q9FN02-2]
    GeneIDi836507.
    GrameneiAT5G63870.1; AT5G63870.1; AT5G63870.
    AT5G63870.2; AT5G63870.2; AT5G63870.
    AT5G63870.3; AT5G63870.3; AT5G63870.
    KEGGiath:AT5G63870.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Entry informationi

    Entry nameiPPP7_ARATH
    AccessioniPrimary (citable) accession number: Q9FN02
    Secondary accession number(s): O49346, Q3E865
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: March 1, 2001
    Last modified: September 27, 2017
    This is version 112 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families