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Protein

BRI1 kinase inhibitor 1

Gene

BKI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of brassinosteroid signaling. When associated to the membrane, limits the interaction of BRI1 with BAK1 by binding to the kinase-inactive form of BRI1.1 Publication

GO - Molecular functioni

  • protein heterodimerization activity Source: TAIR
  • protein kinase inhibitor activity Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Brassinosteroid signaling pathway, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
BRI1 kinase inhibitor 1
Gene namesi
Name:BKI1
Ordered Locus Names:At5g42750
ORF Names:MJB21.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G42750.

Subcellular locationi

  • Cell membrane 1 Publication
  • Cytoplasm 1 Publication

  • Note: Once phosphorylated following brassinosteroid treatment, displaced from the plasma membrane into the cytosol where it is inactive.

GO - Cellular componenti

  • cytosol Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi179 – 1802RK → AA: No effect on subcellular location. Cytosolic only; when associated with 197-A-A-198 or 197-A-A-198 and 209-A-A-210 and 218-A-A-219. 1 Publication
Mutagenesisi197 – 1982KK → AA: Reduced cell membrane localization. Cytosolic only; when associated with 179-A-A-180 or 179-A-A-180 and 209-A-A-210 and 218-A-A-219. 1 Publication
Mutagenesisi209 – 2102KK → AA: Reduced cell membrane localization. Cytosolic only; when associated with 218-A-A-219 or 179-A-A-180 and 197-A-A-198 and 218-A-A-219. 1 Publication
Mutagenesisi211 – 2111Y → D: Cytosolic. Loss of membrane localization in absence of brassinosteroid treatment. 1 Publication
Mutagenesisi211 – 2111Y → F: Loss of cytosolic localization. No dissociation from the membrane upon brassinosteroid treatment. 1 Publication
Mutagenesisi218 – 2192KR → AA: No effect on subcellular location. Cytosolic only; when associated with 209-A-A-210 or 179-A-A-180 and 197-A-A-198 and 209-A-A-210. 1 Publication
Mutagenesisi322 – 3221C → A: No effect on phosphorylation and subcellular location. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 337337BRI1 kinase inhibitor 1PRO_0000410475Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei211 – 2111Phosphotyrosine1 Publication

Post-translational modificationi

Phosphorylated on Tyr-211 in response to brassinosteroid perception, leading to its inactivation: once phosphorylated, displaced into the cytosol where it is inactive.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9FMZ0.

PTM databases

iPTMnetiQ9FMZ0.

Expressioni

Tissue specificityi

Expressed in leaves, petioles, shoot apices, hypocotyls, roots and flowers.1 Publication

Gene expression databases

GenevisibleiQ9FMZ0. AT.

Interactioni

Subunit structurei

Interacts (via C-terminus) with BRI1 (via kinase domain).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
BRL1Q9ZWC84EBI-1111615,EBI-590903

GO - Molecular functioni

  • protein heterodimerization activity Source: TAIR

Protein-protein interaction databases

BioGridi19534. 1 interaction.
IntActiQ9FMZ0. 1 interaction.
STRINGi3702.AT5G42750.1.

Structurei

Secondary structure

1
337
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi308 – 32114Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4OH4X-ray2.25E/F306-325[»]
4Q5JX-ray2.77E/F306-325[»]
ProteinModelPortaliQ9FMZ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi26 – 7045Ser-richAdd
BLAST
Compositional biasi131 – 1355Poly-Asn
Compositional biasi189 – 1946Poly-Gln
Compositional biasi232 – 30675Ser-richAdd
BLAST

Domaini

The c-terminal part (253-337) is necessary and sufficient for the interaction with BRI1.

Phylogenomic databases

eggNOGiENOG410J3Y1. Eukaryota.
ENOG410YVQJ. LUCA.
HOGENOMiHOG000090431.
InParanoidiQ9FMZ0.
KOiK14499.
OMAiFTISLQP.
OrthoDBiEOG09360P45.
PhylomeDBiQ9FMZ0.

Sequencei

Sequence statusi: Complete.

Q9FMZ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METNLQQVKN SSQTFSEKQN PKQEASPSPI SSTCSSPSHD FSFTISLQPL
60 70 80 90 100
SSSSKHISPT LRSPSKTTSS YQQTDPFAVD LSPADEIFFH GHLLPLHLLS
110 120 130 140 150
HLPVSPRTST GSYNDGFTLP VKDILPDQPT NNNNNTENAI TNISTEAKDD
160 170 180 190 200
NTEDKAEGEI RVKTKPIKSF SLFGLSKWRK GFESNEREQE QQQQKIKKPM
210 220 230 240 250
SLDLSHAVKK YIRMLFQKRG NGTQFWNRRQ TSSYSFSSSL MGPNGNSKTM
260 270 280 290 300
INGSYNKRDL IRGRRGELFS APASMRTSPT NSGHLRVSTA GLSSSSGSTS
310 320 330
SSSSDSTMEE LQAAIQAAIA HCKNSSAVDR DDKVKDS
Length:337
Mass (Da):37,104
Last modified:March 1, 2001 - v1
Checksum:i62625FE75B488490
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007647 Genomic DNA. Translation: BAB10631.1.
CP002688 Genomic DNA. Translation: AED94856.1.
AY087216 mRNA. Translation: AAM64772.1.
BT033090 mRNA. Translation: ACF04813.1.
RefSeqiNP_568610.1. NM_123639.2.
UniGeneiAt.30155.

Genome annotation databases

EnsemblPlantsiAT5G42750.1; AT5G42750.1; AT5G42750.
GeneIDi834284.
GrameneiAT5G42750.1; AT5G42750.1; AT5G42750.
KEGGiath:AT5G42750.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007647 Genomic DNA. Translation: BAB10631.1.
CP002688 Genomic DNA. Translation: AED94856.1.
AY087216 mRNA. Translation: AAM64772.1.
BT033090 mRNA. Translation: ACF04813.1.
RefSeqiNP_568610.1. NM_123639.2.
UniGeneiAt.30155.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4OH4X-ray2.25E/F306-325[»]
4Q5JX-ray2.77E/F306-325[»]
ProteinModelPortaliQ9FMZ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19534. 1 interaction.
IntActiQ9FMZ0. 1 interaction.
STRINGi3702.AT5G42750.1.

PTM databases

iPTMnetiQ9FMZ0.

Proteomic databases

PaxDbiQ9FMZ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G42750.1; AT5G42750.1; AT5G42750.
GeneIDi834284.
GrameneiAT5G42750.1; AT5G42750.1; AT5G42750.
KEGGiath:AT5G42750.

Organism-specific databases

TAIRiAT5G42750.

Phylogenomic databases

eggNOGiENOG410J3Y1. Eukaryota.
ENOG410YVQJ. LUCA.
HOGENOMiHOG000090431.
InParanoidiQ9FMZ0.
KOiK14499.
OMAiFTISLQP.
OrthoDBiEOG09360P45.
PhylomeDBiQ9FMZ0.

Miscellaneous databases

PROiQ9FMZ0.

Gene expression databases

GenevisibleiQ9FMZ0. AT.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiBKI1_ARATH
AccessioniPrimary (citable) accession number: Q9FMZ0
Secondary accession number(s): Q8LBG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

BRI1 is not required for BKI1 association with the plasma membrane, but the BRI1 kinase activity is necessary for the membrane release.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.