Reviewed, UniProtKB/Swiss-Prot Q9FMY3 (STPKL_ARATH)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase-like protein At5g23170
    EC=2.7.11.-

Gene names

Ordered Locus Names:	At5g23170
ORF Names:	MKD15.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Tissue specificity	Ubiquitous. Higher expression in mature stamina and pollen. Ref.2
Miscellaneous	This gene is linked with a growth rate QTL (quantitative trait locus).
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAM1	P25854	1	EBI-1239013,EBI-1235664

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Serine/threonine-protein kinase-like protein At5g23170		PRO_0000271405
Regions
	Domain	16 – 298	283	Protein kinase
	Nucleotide binding	22 – 30	9	ATP By similarity
	Coiled coil	311 – 332	22	Potential
	Compositional bias	54 – 69	16	Ser-rich
	Compositional bias	248 – 266	19	Gly-rich
Sites
	Active site	153	1	Proton acceptor By similarity
	Binding site	51	1	ATP By similarity
Natural variations
	Natural variant	40	1	V → D in strain: cv. Bla-10, cv. Bl-0, cv. Can-0, cv. Cnt-1, cv. Cvi-0, cv. Di-G, cv. Ema-1, cv. Ka-0, cv. Kas-1, cv. Landsberg erecta, cv. Lip-0, cv. No-0, cv. Pa-1, cv. Petergof, cv. Sei-0, cv. Sorbo, cv. Tac-0 and cv. Tsu-0.
	Natural variant	45	1	Q → R in strain: cv. Bla-10, cv. Bl-0, cv. Can-0, cv. Cnt-1, cv. Cvi-0, cv. Di-G, cv. Ema-1, cv. Ka-0, cv. Kas-1, cv. Landsberg erecta, cv. Lip-0, cv. No-0, cv. Pa-1, cv. Petergof, cv. Sei-0, cv. Sorbo, cv. Tac-0 and cv. Tsu-0.
	Natural variant	54	1	S → SSL in strain: cv. Per-1, cv. Su-0 and cv. Yo-0.
	Natural variant	56	1	L → LSLSPS in strain: cv. Bla-10, cv. Bl-0, cv. Can-0, cv. Cnt-1, cv. Cvi-0, cv. Di-G, cv. Ema-1, cv. Ka-0, cv. Kas-1, cv. Landsberg erecta, cv. Lip-0, cv. No-0, cv. Pa-1, cv. Petergof, cv. Sei-0, cv. Sorbo, cv. Tac-0 and cv. Tsu-0.
	Natural variant	80	1	D → N in strain: cv. Bla-10, cv. Bl-0, cv. Can-0, cv. Cnt-1, cv. Cvi-0, cv. Di-G, cv. Ema-1, cv. Ka-0, cv. Kas-1, cv. Landsberg erecta, cv. Lip-0, cv. No-0, cv. Pa-1, cv. Petergof, cv. Sei-0, cv. Sorbo, cv. Tac-0 and cv. Tsu-0.
	Natural variant	104	1	V → I in strain: cv. Bla-10, cv. Bl-0, cv. Can-0, cv. Cnt-1, cv. Cvi-0, cv. Di-G, cv. Ema-1, cv. Ka-0, cv. Kas-1, cv. Landsberg erecta, cv. Lip-0, cv. No-0, cv. Pa-1, cv. Petergof, cv. Sei-0, cv. Sorbo, cv. Tac-0 and cv. Tsu-0.
	Natural variant	111	1	E → G in strain: cv. Bla-10, cv. Bl-0, cv. Can-0, cv. Cnt-1, cv. Cvi-0, cv. Di-G, cv. Ema-1, cv. Ka-0, cv. Kas-1, cv. Landsberg erecta, cv. Lip-0, cv. No-0, cv. Pa-1, cv. Petergof, cv. Sei-0, cv. Sorbo, cv. Tac-0 and cv. Tsu-0.
	Natural variant	114	1	Y → H in strain: cv. Bla-10, cv. Bl-0, cv. Cal-0, cv. Can-0, cv. Cnt-1, cv. Cvi-0, cv. Di-G, cv. Di-1, cv. Ema-1, cv. Ka-0, cv. Kas-1, cv. Landsberg erecta, cv. Lip-0, cv. No-0, cv. Pa-1, cv. Per-1, cv. Petergof, cv. Sei-0, cv. Sorbo, cv. Su-0, cv. Tac-0, cv. Tsu-0 and cv. Yo-0.
	Natural variant	126	1	T → A in strain: cv. Di-1.
	Natural variant	142	1	H → N in strain: cv. Bl-0, cv. Cnt-1, cv. Ema-1, cv. Ka-0, cv. Kas-1, cv. No-0, cv. Sei-0 and cv. Tac-0.
	Natural variant	147 – 149	3	HGI → QGV in strain: cv. Bla-10, cv. Bl-0, cv. Can-0, cv. Cnt-1, cv. Cvi-0, cv. Di-G, cv. Ema-1, cv. Ka-0, cv. Kas-1, cv. Landsberg erecta, cv. No-0, cv. Pa-1, cv. Petergof, cv. Sei-0, cv. Sorbo, cv. Tac-0 and cv. Tsu-0.
	Natural variant	177	1	D → E in strain: cv. Bla-10, cv. Bl-0, cv. Can-0, cv. Cnt-1, cv. Cvi-0, cv. Di-G, cv. Ema-1, cv. Ka-0, cv. Kas-1, cv. Landsberg erecta, cv. No-0, cv. Pa-1, cv. Petergof, cv. Sei-0, cv. Sorbo, cv. Tac-0 and cv. Tsu-0.
	Natural variant	237	1	I → M in strain: cv. Sorbo.
	Natural variant	255	1	Missing in strain: cv. Bla-10, cv. Bl-0, cv. Cnt-1, cv. Ema-1, cv. Ka-0, cv. Kas-1, cv. No-0, cv. Pa-1, cv. Sei-0 and cv. Tac-0.
	Natural variant	269	1	L → Q in strain: cv. Bla-10, cv. Bl-0, cv. Cnt-1, cv. Ema-1, cv. Ka-0, cv. Kas-1, cv. No-0, cv. Pa-1, cv. Sei-0 and cv. Tac-0.
	Natural variant	297	1	V → M in strain: cv. Can-0, cv. Cvi-0, cv. Di-G, cv. Landsberg erecta, cv. Lip-0, cv. Petergof, cv. Sorbo and cv. Tsu-0.
	Natural variant	301	1	A → E in strain: cv. Can-0, cv. Cvi-0, cv. Di-G, cv. Landsberg erecta, cv. Lip-0, cv. Petergof, cv. Sorbo and cv. Tsu-0.

Sequences

	Sequence		Length	Mass (Da)
	Q9FMY3-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 7B742E0392C45258	FASTA	341	38,003
	10 20 30 40 50 60 MKEFDYDKLV TAIDRFSPSK LIGKGSHGYV YKALLHHQDV DETRQRVVAI KTPSSLSPSS 70 80 90 100 110 120 PSSSSSSKSE QTKKLENEID VMSSLPYHPH VLSFLGHAEK KLMVVEYMPN ESLYQLLHVS 130 140 150 160 170 180 TDPLPTWLKR IEIALQIASA VHFLHEHGII HRDIKSENIL FDSNWEAKLA DFGLAVDFGG 190 200 210 220 230 240 DKKIRPAPAG TIGYLDPCYT LPENLSMKTD VYSYGVVLLE IVSCRKAIDV SRSPASIVDW 250 260 270 280 290 300 AVPLIKEGRI GEICGGGGGG SGVFRGMSLR LLRMAARCVS SDVESRPCFG EITAEIVACL 310 320 330 340 AEPLKSLPLW MSVLRRVVKL KRRKKRLRET LTWPGQTCRV W

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones." Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:401-414(1997) [PubMed: 9501997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Epistasis and balanced polymorphism influencing complex trait variation." Kroymann J., Mitchell-Olds T. Nature 435:95-98(2005) [PubMed: 15875023] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS, TISSUE SPECIFICITY. Strain: cv. Aa-0, cv. Ag-0, cv. Bl-0, cv. Bla-10, cv. Cal-0, cv. Can-0, cv. Cnt-1, cv. Columbia, cv. Cvi-0, cv. Di-0, cv. Di-1, cv. Di-G, cv. Ema-1, cv. Ka-0, cv. Kas-1, cv. Landsberg erecta, cv. Lip-0, cv. Ma-0, cv. Mt-0, cv. No-0, cv. Oy-0, cv. Pa-1, cv. Per-1, cv. Petergof, cv. Pi-0, cv. Sei-0, cv. Sorbo, cv. Su-0, cv. Tac-0, cv. Tsu-0 and cv. Yo-0.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB007648 Genomic DNA. Translation: BAB11172.1. AJ864997 Genomic DNA. Translation: CAI23745.1. AJ864994 Genomic DNA. Translation: CAI23739.1. AJ864992 Genomic DNA. Translation: CAI23737.1. AJ864991 Genomic DNA. Translation: CAI23735.1. AJ864983 Genomic DNA. Translation: CAI23717.1. AJ864976 Genomic DNA. Translation: CAI23703.1. AJ864969 Genomic DNA. Translation: CAI23691.1. AJ864968 Genomic DNA. Translation: CAI23689.1. AJ864980 Genomic DNA. Translation: CAI23711.1. AJ864984 Genomic DNA. Translation: CAI23720.1. AJ864973 Genomic DNA. Translation: CAI23697.1. AJ864996 Genomic DNA. Translation: CAI23743.1. AJ864972 Genomic DNA. Translation: CAI23695.1. AJ864990 Genomic DNA. Translation: CAI23733.1. AJ864971 Genomic DNA. Translation: CAI23693.1. AJ864989 Genomic DNA. Translation: CAI23731.1. AJ864985 Genomic DNA. Translation: CAI23723.1. AJ864982 Genomic DNA. Translation: CAI23715.1. AJ864981 Genomic DNA. Translation: CAI23713.1. AJ864970 Genomic DNA. Translation: CAI23749.1. AJ864987 Genomic DNA. Translation: CAI23727.1. AJ864995 Genomic DNA. Translation: CAI23741.1. AJ864979 Genomic DNA. Translation: CAI23709.1. AJ864975 Genomic DNA. Translation: CAI23701.1. AJ864998 Genomic DNA. Translation: CAI23747.1. AJ864993 Genomic DNA. Translation: CAI23751.1. AJ864988 Genomic DNA. Translation: CAI23729.1. AJ864986 Genomic DNA. Translation: CAI23725.1. AJ864978 Genomic DNA. Translation: CAI23707.1. AJ864977 Genomic DNA. Translation: CAI23705.1. AJ864974 Genomic DNA. Translation: CAI23699.1. BT005316 mRNA. Translation: AAO63380.1. AK117152 mRNA. Translation: BAC41830.1.
IPI	IPI00532007.
RefSeq	NP_197708.1.
UniGene	At.31022
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9FMY3. 1 interaction.
Genome annotation databases
GeneID	832381.
GenomeReviews	Gene locus AT5G23170 in contig BA000015_GR.
KEGG	ath:AT5G23170.
NMPDR	fig|3702.1.peg.24488.
Organism-specific databases
GeneFarm	2038. 161.
TAIR	At5g23170.
Phylogenomic databases
OMA	Q9FMY3. YLDPCYT.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

STPKL_ARATH

Accession

Primary (citable) accession number: Q9FMY3
Secondary accession number(s): Q50IB8 , Q50IC8, Q50ID4, Q50IE0, Q50IE8, Q50IF7, Q50IG2, Q50IG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)