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Q9FMW9

- ICMTA_ARATH

UniProt

Q9FMW9 - ICMTA_ARATH

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Protein

Protein-S-isoprenylcysteine O-methyltransferase A

Gene

ICMTA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues, resulting in the modulation of the function of prenylated proteins. Involved in negative regulation of abscisic acid signaling. Carboxyl methylation is a reversible and potentially regulated step in the post-translational modification of prenylated proteins.4 Publications

Catalytic activityi

S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.

Cofactori

Divalent cations. Probably zinc (By similarity).By similarity

Enzyme regulationi

Inhibited by farnesylthioacetic acid (FTAA) and N-acetyl-S-trans, trans-farnesyl-l-cysteine (AFC).

Kineticsi

  1. KM=22.7 µM for AFC as methyl acceptor1 Publication
  2. KM=13.7 µM for AGGC as methyl acceptor1 Publication

Vmax=5.0 pmol/min/mg enzyme toward AFC as methyl acceptor1 Publication

Vmax=3.7 pmol/min/mg enzyme toward AGGC as methyl acceptor1 Publication

GO - Molecular functioni

  1. carboxyl-O-methyltransferase activity Source: TAIR
  2. protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity Source: TAIR

GO - Biological processi

  1. C-terminal protein methylation Source: TAIR
  2. flower development Source: TAIR
  3. negative regulation of abscisic acid-activated signaling pathway Source: TAIR
  4. shoot system development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciARA:AT5G23320-MONOMER.
MetaCyc:AT5G23320-MONOMER.
SABIO-RKQ9FMW9.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-S-isoprenylcysteine O-methyltransferase A (EC:2.1.1.100)
Short name:
AtICMTA
Alternative name(s):
Isoprenylcysteine carboxylmethyltransferase A
Prenylated protein carboxyl methyltransferase A
Prenylcysteine carboxyl methyltransferase A
Short name:
AtPCM
Gene namesi
Name:ICMTA
Synonyms:PCM, STE14, STE14A
Ordered Locus Names:At5g23320
ORF Names:MKD15.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G23320.

Subcellular locationi

Endoplasmic reticulum membrane 2 Publications; Multi-pass membrane protein 2 Publications

GO - Cellular componenti

  1. endoplasmic reticulum Source: TAIR
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype; due to redundancy with ICMTB. Icmta and icmtb double mutants have altered phyllotaxis, fasciated stems and development of axillary flowers.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi111 – 1111N → R: No effect; when associated with R-112 and E-165. Activity increased to the level of that of ICMTB; when associated with R-112; E-165; Q-187 and R-188. 1 Publication
Mutagenesisi112 – 1121Y → R: No effect; when associated with R-111 and E-165. Activity increased to the level of that of ICMTB; when associated with R-111; E-165; Q-187 and R-188. 1 Publication
Mutagenesisi165 – 1651Q → E: No effect. No effect; when associated with R-111 and R-112. Activity increased to the level of that of ICMTB; when associated with R-111; R-112; Q-187 and R-188. 1 Publication
Mutagenesisi187 – 1871E → Q: Activity increased to the level of that of ICMTB; when associated with R-111; R-112; E-165 and R-188. 1 Publication
Mutagenesisi188 – 1881S → R: Activity increased to the level of that of ICMTB; when associated with R-111; R-112; E-165 and Q-187. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 197197Protein-S-isoprenylcysteine O-methyltransferase APRO_0000356249Add
BLAST

Expressioni

Tissue specificityi

Expressed primarily in flowers, stems, leaves and roots. Almost not expressed in siliques. Detected in root tips and vascular tissues of roots, cotyledons, petiols, hypocotyls, filaments, pollen grains and the distal and proximal portions of the gynoecium.2 Publications

Inductioni

Not induced by abscisic acid or auxin.1 Publication

Gene expression databases

GenevestigatoriQ9FMW9.

Structurei

3D structure databases

ProteinModelPortaliQ9FMW9.
SMRiQ9FMW9. Positions 101-185.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei16 – 3621HelicalSequence AnalysisAdd
BLAST
Transmembranei52 – 7221HelicalSequence AnalysisAdd
BLAST
Transmembranei81 – 10121HelicalSequence AnalysisAdd
BLAST
Transmembranei140 – 16021HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2020.
HOGENOMiHOG000213961.
InParanoidiQ9FMW9.
KOiK00587.
OMAiHGVYRLM.
PhylomeDBiQ9FMW9.

Family and domain databases

InterProiIPR007269. ICMT_MeTrfase.
IPR025770. PPMT_MeTrfase.
[Graphical view]
PfamiPF04140. ICMT. 1 hit.
[Graphical view]
PROSITEiPS51564. SAM_ICMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9FMW9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEIFSDTSI RQLSQMLLSL IFFHISEYIL AITIHGASNV TLSSLLITKH
60 70 80 90 100
YALAMLLSLL EYLTEIILFP GLKQHWWVSN FGLIMIIVGE IIRKAAIITA
110 120 130 140 150
GRSFTHLIKI NYEEHHGLVT HGVYRLMRHP SYCGFLIWSV GTQVMLCNPV
160 170 180 190
SAVAFAVVVW RFFAQRIPYE EYFLNQFFGV QYLEYAESVA SGVPFVN
Length:197
Mass (Da):22,525
Last modified:March 1, 2001 - v1
Checksum:i11ED13E628D3A47B
GO

Sequence cautioni

The sequence ABK28709.1 differs from that shown. Reason: Erroneous termination at position 198. Translated as stop.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB007648 Genomic DNA. Translation: BAB11187.1.
CP002688 Genomic DNA. Translation: AED93151.1.
DQ446977 mRNA. Translation: ABE66176.1.
DQ653301 mRNA. Translation: ABK28709.1. Sequence problems.
RefSeqiNP_197723.1. NM_122238.1.
UniGeneiAt.54958.

Genome annotation databases

EnsemblPlantsiAT5G23320.1; AT5G23320.1; AT5G23320.
GeneIDi832396.
KEGGiath:AT5G23320.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB007648 Genomic DNA. Translation: BAB11187.1 .
CP002688 Genomic DNA. Translation: AED93151.1 .
DQ446977 mRNA. Translation: ABE66176.1 .
DQ653301 mRNA. Translation: ABK28709.1 . Sequence problems.
RefSeqi NP_197723.1. NM_122238.1.
UniGenei At.54958.

3D structure databases

ProteinModelPortali Q9FMW9.
SMRi Q9FMW9. Positions 101-185.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G23320.1 ; AT5G23320.1 ; AT5G23320 .
GeneIDi 832396.
KEGGi ath:AT5G23320.

Organism-specific databases

TAIRi AT5G23320.

Phylogenomic databases

eggNOGi COG2020.
HOGENOMi HOG000213961.
InParanoidi Q9FMW9.
KOi K00587.
OMAi HGVYRLM.
PhylomeDBi Q9FMW9.

Enzyme and pathway databases

BioCyci ARA:AT5G23320-MONOMER.
MetaCyc:AT5G23320-MONOMER.
SABIO-RK Q9FMW9.

Gene expression databases

Genevestigatori Q9FMW9.

Family and domain databases

InterProi IPR007269. ICMT_MeTrfase.
IPR025770. PPMT_MeTrfase.
[Graphical view ]
Pfami PF04140. ICMT. 1 hit.
[Graphical view ]
PROSITEi PS51564. SAM_ICMT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Carboxyl-methylation of prenylated calmodulin CaM53 is required for efficient plasma membrane targeting of the protein."
    Rodriguez-Concepcion M., Toledo-Ortiz G., Yalovsky S., Caldelari D., Gruissem W.
    Plant J. 24:775-784(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
  2. "Identification and functional expression in yeast of a prenylcysteine alpha-carboxyl methyltransferase gene from Arabidopsis thaliana."
    Crowell D.N., Kennedy M.
    Plant Mol. Biol. 45:469-476(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
    Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations."
    Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.
    Plant Biotechnol. J. 4:317-324(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Prenylcysteine alpha-carboxyl methyltransferase expression and function in Arabidopsis thaliana."
    Narasimha Chary S., Bultema R.L., Packard C.E., Crowell D.N.
    Plant J. 32:735-747(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
  7. "Functional analysis of Arabidopsis postprenylation CaaX processing enzymes and their function in subcellular protein targeting."
    Bracha-Drori K., Shichrur K., Lubetzky T.C., Yalovsky S.
    Plant Physiol. 148:119-131(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF ASN-111; TYR-112; GLN-165; GLU-187 AND SER-188, DISRUPTION PHENOTYPE.
  8. "Isoprenylcysteine methylation and demethylation regulate abscisic acid signaling in Arabidopsis."
    Huizinga D.H., Omosegbon O., Omery B., Crowell D.N.
    Plant Cell 20:2714-2728(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.

Entry informationi

Entry nameiICMTA_ARATH
AccessioniPrimary (citable) accession number: Q9FMW9
Secondary accession number(s): A0MFH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

ICMTA is less widely expressed and has a lower catalytic activity than ICMTB.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3