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Q9FMU6 (MPCP3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial phosphate carrier protein 3, mitochondrial
Alternative name(s):
Mitochondrial phosphate transporter 3
Short name=MPT3
Phosphate transporter 3;1
Gene names
Name:MPT3
Synonyms:AT5, PHT3;1
Ordered Locus Names:At5g14040
ORF Names:MUA22_4
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length375 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transport of phosphate groups from the cytosol to the mitochondrial matrix. Mediates salt stress tolerance through an ATP-dependent pathway and via modulation of the gibberellin metabolism. Ref.6 Ref.11

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein Ref.7 Ref.8.

Tissue specificity

Expressed in stems, leaves and flowers. Strong expression in vascular tissues. Ref.11

Induction

By salt stress. Ref.11

Miscellaneous

Plants overexpressing MPT3/PHT3;1 display increased sensitivity to salt stress (Ref.11).

Sequence similarities

Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification]

Contains 3 Solcar repeats.

Sequence caution

The sequence BAD94852.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 375Mitochondrial phosphate carrier protein 3, mitochondrialPRO_0000421697

Regions

Topological domain? – 75Mitochondrial intermembrane Potential
Transmembrane76 – 9621Helical; Name=1; Potential
Topological domain97 – 13438Mitochondrial matrix Potential
Transmembrane135 – 15420Helical; Name=2; Potential
Topological domain155 – 17521Mitochondrial intermembrane Potential
Transmembrane176 – 19621Helical; Name=3; Potential
Topological domain197 – 23135Mitochondrial matrix Potential
Transmembrane232 – 25120Helical; Name=4; Potential
Topological domain252 – 27221Mitochondrial intermembrane Potential
Transmembrane273 – 29321Helical; Name=5; Potential
Topological domain294 – 33239Mitochondrial matrix Potential
Transmembrane333 – 35321Helical; Name=6; Potential
Topological domain354 – 37522Mitochondrial intermembrane Potential
Repeat76 – 16085Solcar 1
Repeat173 – 25785Solcar 2
Repeat274 – 35380Solcar 3

Amino acid modifications

Modified residue1041S-nitrosocysteine Ref.10

Sequences

Sequence LengthMass (Da)Tools
Q9FMU6 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: C58B3057954C9CF6

FASTA37540,090
        10         20         30         40         50         60 
MESPKNSLIP SFLYSSSSSP RSFLLDQVLN SNSNAAFEKS PSPAPRSSPT SMISRKNFLI 

        70         80         90        100        110        120 
ASPTEPGKGI EMYSPAFYAA CTFGGILSCG LTHMTVTPLD LVKCNMQIDP AKYKSISSGF 

       130        140        150        160        170        180 
GILLKEQGVK GFFRGWVPTL LGYSAQGACK FGFYEYFKKT YSDLAGPEYT AKYKTLIYLA 

       190        200        210        220        230        240 
GSASAEIIAD IALCPFEAVK VRVQTQPGFA RGMSDGFPKF IKSEGYGGLY KGLAPLWGRQ 

       250        260        270        280        290        300 
IPYTMMKFAS FETIVEMIYK YAIPNPKSEC SKGLQLGVSF AGGYVAGVFC AIVSHPADNL 

       310        320        330        340        350        360 
VSFLNNAKGA TVGDAVKKIG MVGLFTRGLP LRIVMIGTLT GAQWGLYDAF KVFVGLPTTG 

       370 
GVAPAPAIAA TEAKA 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones."
Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.
DNA Res. 4:401-414(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Isolation and characterization of cDNAs encoding mitochondrial phosphate transporters in soybean, maize, rice, and Arabidopis."
Takabatake R., Hata S., Taniguchi M., Kouchi H., Sugiyama T., Izui K.
Plant Mol. Biol. 40:479-486(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 88-375.
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 220-375.
Strain: cv. Columbia.
[6]"Redundancy in the function of mitochondrial phosphate transport in Saccharomyces cerevisiae and Arabidopsis thaliana."
Hamel P., Saint-Georges Y., de Pinto B., Lachacinski N., Altamura N., Dujardin G.
Mol. Microbiol. 51:307-317(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"The hydrophobic proteome of mitochondrial membranes from Arabidopsis cell suspensions."
Brugiere S., Kowalski S., Ferro M., Seigneurin-Berny D., Miras S., Salvi D., Ravanel S., d'Herin P., Garin J., Bourguignon J., Joyard J., Rolland N.
Phytochemistry 65:1693-1707(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"The growing family of mitochondrial carriers in Arabidopsis."
Picault N., Hodges M., Palmieri L., Palmieri F.
Trends Plant Sci. 9:138-146(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[10]"Proteomics investigation of endogenous S-nitrosylation in Arabidopsis."
Fares A., Rossignol M., Peltier J.B.
Biochem. Biophys. Res. Commun. 416:331-336(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: S-NITROSYLATION AT CYS-104.
[11]"The mitochondrial phosphate transporters modulate plant responses to salt stress via affecting ATP and gibberellin metabolism in Arabidopsis thaliana."
Zhu W., Miao Q., Sun D., Yang G., Wu C., Huang J., Zheng C.
PLoS ONE 7:E43530-E43530(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, TISSUE SPECIFICITY, INDUCTION BY SALT, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB007650 Genomic DNA. Translation: BAB08283.1.
CP002688 Genomic DNA. Translation: AED91980.1.
AY058848 mRNA. Translation: AAL24236.1.
AY143869 mRNA. Translation: AAN28808.1.
AB016066 mRNA. Translation: BAA31585.1.
AK222060 mRNA. Translation: BAD94852.1. Different initiation.
PIRT51595.
RefSeqNP_196908.1. NM_121407.2.
UniGeneAt.47569.
At.486.

3D structure databases

ProteinModelPortalQ9FMU6.
SMRQ9FMU6. Positions 83-355.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9FMU6. 1 interaction.
STRING3702.AT5G14040.1-P.

Protein family/group databases

TCDB2.A.29.4.6. the mitochondrial carrier (mc) family.

Proteomic databases

PRIDEQ9FMU6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G14040.1; AT5G14040.1; AT5G14040.
GeneID831252.
KEGGath:AT5G14040.

Organism-specific databases

GeneFarm3804. 380.
TAIRAT5G14040.

Phylogenomic databases

eggNOGNOG265500.
HOGENOMHOG000164438.
InParanoidQ9FMU6.
KOK15102.
OMAWKTSLYL.
PhylomeDBQ9FMU6.

Gene expression databases

GenevestigatorQ9FMU6.

Family and domain databases

Gene3D1.50.40.10. 1 hit.
InterProIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamPF00153. Mito_carr. 2 hits.
[Graphical view]
SUPFAMSSF103506. SSF103506. 1 hit.
PROSITEPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPCP3_ARATH
AccessionPrimary (citable) accession number: Q9FMU6
Secondary accession number(s): O80415, Q56WI0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: March 1, 2001
Last modified: June 11, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names