Reviewed,
UniProtKB/Swiss-Prot Q9FMT1 (LEU33_ARATH)
Last modified
November 4, 2008.
Version 52.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 3-isopropylmalate dehydrogenase 3, chloroplastic Short name=3-IPM-DH 3 Short name=IMDH 3 EC=1.1.1.85 Alternative name(s): Beta-IPM dehydrogenase 3 | ||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 409 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. |
| Catalytic activity | (2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH. |
| Cofactor | Binds 1 magnesium or manganese ion per subunit By similarity. |
| Pathway | Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. |
| Subunit structure | Homodimer By similarity. |
| Subcellular location | Plastid › chloroplastBy similarity. |
| Sequence similarities | Belongs to the isocitrate and isopropylmalate dehydrogenases family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis |
| Cellular component | Chloroplast Plastid |
| Coding sequence diversity | Alternative splicing |
| Domain | Transit peptide |
| Ligand | Magnesium Manganese Metal-binding NAD |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | chloroplast Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Alternative products
| This entry describes 1 isoform produced by alternative splicing. [Select] Notes: A number of isoforms are produced. According to EST sequences. | ||||||
| Isoform 1 (identifier: Q9FMT1-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 37 | 37 | Chloroplast Potential | ||||||
| Chain | 38 – 409 | 372 | 3-isopropylmalate dehydrogenase 3, chloroplastic | PRO_0000014455 | |||||
Regions | |||||||||
| Nucleotide binding | 120 – 133 | 14 | NAD By similarity | ||||||
| Nucleotide binding | 326 – 338 | 13 | NAD By similarity | ||||||
Sites | |||||||||
| Metal binding | 268 | 1 | Magnesium or manganese By similarity | ||||||
| Metal binding | 292 | 1 | Magnesium or manganese By similarity | ||||||
| Metal binding | 296 | 1 | Magnesium or manganese By similarity | ||||||
| Binding site | 140 | 1 | Substrate By similarity | ||||||
| Binding site | 150 | 1 | Substrate By similarity | ||||||
| Binding site | 178 | 1 | Substrate By similarity | ||||||
| Binding site | 268 | 1 | Substrate By similarity | ||||||
| Site | 185 | 1 | Important for catalysis By similarity | ||||||
| Site | 236 | 1 | Important for catalysis By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 251 | 1 | T → A in AAL67125. Ref.2 | ||||||
Sequences
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References
| [1] | "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones." Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:401-414(1997) [PubMed: 9501997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [2] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
Cross-references
Sequence databases | |
|---|---|
| AB007650 Genomic DNA. Translation: BAB08299.1. AY074587 mRNA. Translation: AAL67125.1. | |
| RefSeq | NP_196924.1. |
| UniGene | At.6515 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1A05 based on UniProtKB Q56268. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 831270. |
| GenomeReviews | Gene locus AT5G14200 in contig BA000015_GR. |
| NMPDR | fig|3702.1.peg.23549. |
Organism-specific databases | |
| TAIR | At5g14200. |
Family and domain databases | |
| InterPro | IPR004429. 3-isopropylmalate_DHase. IPR001804. IsoCit_IM_DHase. [Graphical view] |
| Gene3D | G3DSA:3.40.718.10. IDH_IMDH. 1 hit. |
| PANTHER | PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit. |
| Pfam | PF00180. Iso_dh. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00169. leuB. 1 hit. |
| PROSITE | PS00470. IDH_IMDH. 1 hit. [Graphical view] |
| BLOCKS | Search... |
| ProtoNet | Search... |
Entry information
| Entry name | LEU33_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q9FMT1 Secondary accession number(s): Q8VXU4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| UniProtKB secondary accession numbers Index of UniProtKB secondary accession numbers |
| SIMILARITY comments Index of protein domains and families |

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